Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggygria extracts

Esra Bozkaya; Mustafa Türk; Hüsamettin Ekici; Siyami Karahan

doi:10.33988/auvfd.1217177

Research Article

Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggygria extracts

Year 2024, , 269 - 280, 10.07.2024

Esra Bozkaya , Mustafa Türk , Hüsamettin Ekici , Siyami Karahan

https://doi.org/10.33988/auvfd.1217177

Abstract

Cotinus coggygria is widely recognized its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this, aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities, and in vivo wound healing effects of C. coggygria, a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the methanol extract of C. coggygria leaves contained flavonoids and phenolic compounds such as gallic acid (18.5 mg/kg), catechin (4.6 mg/kg), protocatesic acid (0.6 mg/kg), vanillic acid (8.4 mg/kg), ellagic acid (0.1 mg/kg), rosmarinic acid (0.1 mg/kg), quercetin (15 ppb) and C. coggygria stems contained such as gallic acid (24.6 mg/kg), catechin (155.1 mg/kg), chlorogenic acid (1.9 mg/kg), 4-hydroxybenzoic acid (383.3 mg/kg), rutin (2.5 mg/kg), ellagic acid (15.1 mg/kg), apigenin 7-glycoside (10.5 mg/kg), rosmarinic acid (0,4 mg/kg), quercetin (15.2 mg/kg), naringenin (279.1 mg/kg). Consequently, C. coggyria has a positive effect on wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, or hemolytic effects at test concentrations. In the in vivo burn model, wounds treated with leaf and stem extracts healed faster than the control group. Thus, C. coggygria is an effective plant for wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, and hemolytic effects.

Keywords

cytotoxicity, Cotinus coggyria, genotoxicity, hemolysis, In vivo wound healing

Ethical Statement

The study was approved by Kırıkkale University Animal Experiments Local Ethics Committee with the decision dated 16.12.2013, numbered 2013/15, meeting numbered 13/02.

Supporting Institution

This study was financially supported by the Ministry of Science, Industry and Technology SANTEZ Project No. 0352.STZ.2013-2 of the Ministry of Science, Industry and Technology.

Project Number

0352.STZ.2013-2

Thanks

This study constitutes a part of Esra Arat’s (Bozkaya) doctoral dissertation studies entitled “Development of Easily Applicable Herbal Products to Promote Tissue Regeneration and Ecm Synthesis” at Kırıkkale University, Insitute of Science, Biology doctorate program. We would like to thank Kırıkkale University Scientific and Technological Research Application and Research Center for their support.

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