Histochemical and immunohistochemical investigations on pyloric tonsil in Turkeys (Meleagris gallopavo)
Abstract
The structural patterns of pyloric tonsil in turkey (Meleagris gallopavo) were investigated in this study. Turkeys, less resistant to disease than chickens, are a crucial source of protein with low cholesterol levels and a high protein/calorie ratio. Pyloric tonsil, involved in the chicken defense barrier, is a component of GALT. To evaluate the specific region, adult turkeys were taken from slaughterhouses. Crossman's modified triple staining demonstrated the general histological structure, PAS revealed the density of goblet cells synthesizing mucus, and Safranin O showed the capsule around the follicles. The width of lymphoid follicles in the pyloric tonsil (204.0±22.33) differed significantly from that of the middle part of the duodenum (111.7±4.741). It was noticed that the specific area harbors T cells, B cells, and follicular dendritic cells. Interfollicular regions were infiltrated with CD3+T cells, but the CD268+B cells occupied primarily germinal centers. However, it was noticed that T cells were present in germinal centers and some B cells in the interfollicular region. Anti-Vimentin antibody revealed follicular dendritic cells in the pyloric zone. It was noted that the epithelial layer of the pyloric tonsil is devoid of CK18-positive cells. Findings of the semi-thin section indicated that some columnar epithelial cells were stained differently in the transition zone (lymphoepithelium). To sum up, a pyloric region in turkeys is structurally dissimilar to pyloric tonsils detected in chicken, chukar partridge, and duck. More research is needed to reveal the effect of pyloric tonsil on the alimentary tract in poultry in terms of defense mechanisms.
Keywords
Supporting Institution
This research was supported by Ankara University Scientific Research Grant No: 17L0239011.
Project Number
Grant number:17L0239011
Thanks
This study is produced from author’s PhD thesis and presented as an oral presentation at at 8th International Congress on Innovative Scientific Approaches, 23-24.11.2022, Samsun, Türkiye. For his contribution to obtaining and interpreting the findings of this study, I thank Prof. Dr. Hikmet ALTUNAY.
References
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- Bancroft JD, Gamble M (2008): Theory and Practice of Histological Techniques. 6th edn. Churchill Livingstone, Elsevier, China.
- Bucy RP, Chen CL, Cihak J, et al (1988): Avian T cells expressing γδ receptors localize in the splenic sinusoids and the intestinal epithelium. J Immunol, 141, 2200-2205.
- Casteleyn C, Doom M, Lambrechts E, et al (2010): Locations of gut-associated lymphoid tissue in the 3-monthold chicken: a review. Avian Pathol, 39, 143–150.
- Davison F, Kaspers B, Schat KA, et al (2011): Avian Immunology. 1st edn. MA: Elsevier/Academic Press, Boston.
- Decaestecker E, Gaba S, Raeymaekers JA, et al (2007): Host-parasite 'Red Queen' dynamics archived in pond sediment. Nature, 450, 870-873.
- Eurell JA, Frappier BL (2006): Textbook of Veterinary Histology, 6th edn. Blackwell Publishing Ltd, Oxford.
- Fagerland JA, Arp LH (1993): Structure and development of bronchus-associated lymphoid tissue in conventionally reared broiler chickens. Avian Dis, 37, 10-18.
- Gartner LP, Hiatt JL (1997): Digestive system II–Alimentary Canal. Color Textbook of Histology. 1st edn. PA: Saunders, Philadelphia.
- Gedam PM, Nandeshwar NC, Salankar AM, et al (2018): Histomorphological and histochemical studies on lymphoid tissue of pyloric region in khaki campbell breed of duck (Anas platyrhynchos). J Entomol Zool Stud, 6, 1222-1224.
- Guy-Grand D, Cerf-Bensussen N, Malissen B, et al (1991): Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation. J Exp Med, 173, 471-481.
- Hondo T, Kanaya T, Takakura I, et al (2011): Cytokeratin 18 is a specific marker of bovine intestinal M cell. Am J Physiol Gastrointest Liver Physiol, 300, G442-G453.
- Kido T, Ohwada S, Watanabe K, et al (2003): Differential characteristics of microfold cells on the dome epithelium of porcine ileum. Anim Sci J, 74, 375-382.
- Mason DY, Cordell J L, Brown MH, et al (1995): CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood, 86, 1453-1459.
- Mörbe UM, Jørgensen PB, Fenton TM, et al (2021): Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol, 14, 793-802.
- Nagy N, Oláh I (2007): Pyloric tonsil as a novel gut associated lymphoepithelial organ of the chicken. J Anat, 211, 407-411.
- Oláh I, Nagy N, Magyar A, et al (2003): Esophageal tonsil: a novel gut associated lymphoid organ. Poultry Sci, 82, 767–770.
- Oláh I, Nagy N, Vervelde L (2014): Structure of the avian lymphoid system. 11-44. In: Avian Immunology. F Davison (Ed), B Kaspers (Ed), KA Schat (Ed), P Kaiser (Ed). Elsevier, Philadelphia.
- Perry ME (1994): The specialised structure of crypt epithelium in the human palatine tonsil and its functional significance. J Anat, 185, 111-127.
- Reddy SK, Hu T, Gudivada R, et al (2008): The BAFF-Interacting receptors of chickens. Dev Comp Immunol, 32, 1076-1087.
- Samuelson DA (2007): Textbook of Veterinary Histology. Saunders– Elsevier, St. Louis, Missouri.
- Somayeh H JL (2021): Histological characterization of the gut-associated lymphoid tissue in three-month old guinea fowls (Numida meleagris). Philipp J Vet Med, 58, 96-100.
- van der Poel CE, Bajic G, Macaulay CW, et al (2019): Follicular dendritic cells modulate germinal center B cell diversity through FcγRIIB. Cell Rep, 29, 2745-2755.
- Vervelde L, Jeurissen SHM (1993): Postnatal development of intra-epithelial leukocytes in the chicken digestive tract: phenotypical characterisation in situ. Cell Tissue Res, 274, 295-301.
- Woolhouse ME, Webster JP, Domingo E, et al (2002): Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet, 32, 569-577.
Abstract
Keywords
Supporting Institution
Ankara University Scientific Research Grant
Project Number
Grant number:17L0239011
Thanks
Metin içerisinde belirtilmiştir.
References
- Arugh PA, Hamedi S (2019): A histomorphometric study on age-related changes in selected lymphoid structures of Chukar partridge (Alectoris chukar). Iran J Vet Res, 20, 186-191.
- Bancroft JD, Gamble M (2008): Theory and Practice of Histological Techniques. 6th edn. Churchill Livingstone, Elsevier, China.
- Bucy RP, Chen CL, Cihak J, et al (1988): Avian T cells expressing γδ receptors localize in the splenic sinusoids and the intestinal epithelium. J Immunol, 141, 2200-2205.
- Casteleyn C, Doom M, Lambrechts E, et al (2010): Locations of gut-associated lymphoid tissue in the 3-monthold chicken: a review. Avian Pathol, 39, 143–150.
- Davison F, Kaspers B, Schat KA, et al (2011): Avian Immunology. 1st edn. MA: Elsevier/Academic Press, Boston.
- Decaestecker E, Gaba S, Raeymaekers JA, et al (2007): Host-parasite 'Red Queen' dynamics archived in pond sediment. Nature, 450, 870-873.
- Eurell JA, Frappier BL (2006): Textbook of Veterinary Histology, 6th edn. Blackwell Publishing Ltd, Oxford.
- Fagerland JA, Arp LH (1993): Structure and development of bronchus-associated lymphoid tissue in conventionally reared broiler chickens. Avian Dis, 37, 10-18.
- Gartner LP, Hiatt JL (1997): Digestive system II–Alimentary Canal. Color Textbook of Histology. 1st edn. PA: Saunders, Philadelphia.
- Gedam PM, Nandeshwar NC, Salankar AM, et al (2018): Histomorphological and histochemical studies on lymphoid tissue of pyloric region in khaki campbell breed of duck (Anas platyrhynchos). J Entomol Zool Stud, 6, 1222-1224.
- Guy-Grand D, Cerf-Bensussen N, Malissen B, et al (1991): Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation. J Exp Med, 173, 471-481.
- Hondo T, Kanaya T, Takakura I, et al (2011): Cytokeratin 18 is a specific marker of bovine intestinal M cell. Am J Physiol Gastrointest Liver Physiol, 300, G442-G453.
- Kido T, Ohwada S, Watanabe K, et al (2003): Differential characteristics of microfold cells on the dome epithelium of porcine ileum. Anim Sci J, 74, 375-382.
- Mason DY, Cordell J L, Brown MH, et al (1995): CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood, 86, 1453-1459.
- Mörbe UM, Jørgensen PB, Fenton TM, et al (2021): Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol, 14, 793-802.
- Nagy N, Oláh I (2007): Pyloric tonsil as a novel gut associated lymphoepithelial organ of the chicken. J Anat, 211, 407-411.
- Oláh I, Nagy N, Magyar A, et al (2003): Esophageal tonsil: a novel gut associated lymphoid organ. Poultry Sci, 82, 767–770.
- Oláh I, Nagy N, Vervelde L (2014): Structure of the avian lymphoid system. 11-44. In: Avian Immunology. F Davison (Ed), B Kaspers (Ed), KA Schat (Ed), P Kaiser (Ed). Elsevier, Philadelphia.
- Perry ME (1994): The specialised structure of crypt epithelium in the human palatine tonsil and its functional significance. J Anat, 185, 111-127.
- Reddy SK, Hu T, Gudivada R, et al (2008): The BAFF-Interacting receptors of chickens. Dev Comp Immunol, 32, 1076-1087.
- Samuelson DA (2007): Textbook of Veterinary Histology. Saunders– Elsevier, St. Louis, Missouri.
- Somayeh H JL (2021): Histological characterization of the gut-associated lymphoid tissue in three-month old guinea fowls (Numida meleagris). Philipp J Vet Med, 58, 96-100.
- van der Poel CE, Bajic G, Macaulay CW, et al (2019): Follicular dendritic cells modulate germinal center B cell diversity through FcγRIIB. Cell Rep, 29, 2745-2755.
- Vervelde L, Jeurissen SHM (1993): Postnatal development of intra-epithelial leukocytes in the chicken digestive tract: phenotypical characterisation in situ. Cell Tissue Res, 274, 295-301.
- Woolhouse ME, Webster JP, Domingo E, et al (2002): Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet, 32, 569-577.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Histology and Embryology |
| Journal Section | Research Article |
| Authors | |
| Project Number | Grant number:17L0239011 |
| Early Pub Date | September 21, 2023 |
| Publication Date | July 10, 2024 |
| Published in Issue | Year 2024 |