Abstract
Bu çalışmada, farklı dozlarda bor (sodyum pentaborat) içeren boğa sperması sulandırıcılarının çözüm sonu toplam motilite (TMOT), progresif motilite (PMOT), spermatozoa hareket parametreleri (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plazma membranı ve akrozom bütünlüğü (PMAI) ve DNA fragmentasyon indeksi (DFI) parametreleri üzerine etkisi değerlendirildi. Dört baş boğadan toplam 8 ejakülat incelendi. Her ejakülat 5 eşit parçaya bölündü ve kontrol grubu olarak standart Tris sulandırıcısıyla sulandırıldı. Deney grubu sulandırıcıları olarak 0,5 g glikoza ek olarak 0,4 g bor eklenmiş Tris sulandırıcısı (Grup 1) ve glikoz yerine 0.5; 0.7; 0.9 g bor eklenmiş Tris sulandırıcıları (Grup 2, Grup 3, Grup 4) kullanıldı. Spermalar çözdürüldükten sonra TMOT, PMOT, spermatozoa hareket parametreleri, PMAI ve DFI değerleri belirlendi. Toplam motilite ve progresif motilite verilerine göre (%66.04, %50.79) Grup 1 sulandırıcısının daha yüksek değerlerde olmasına rağmen gruplar arası farklılıklar istatistiki açıdan önemsiz bulundu (P>0.05). En yüksek VSL, LIN, STR ve WOB değerlerinin (28.15 µm/s, %42.98, %71.69, %59.71) Grup 1’de olduğu görüldü (P<0.05). Diğer hareket özelliklerinin de Grup 1’de diğer gruplara göre yüksek olduğu belirlenirken, gruplar arası farklılıklar istatistiki açıdan önemsiz bulundu (P>0.05). En yüksek PMAI değerinin Grup 1’de olduğu, DFI değerlerinin ise tüm gruplarda benzer olduğu sonucuna ulaşıldı (P>0.05). Sonuç olarak borun çözdürme işleminden sonra spermatozoanın kinetik ve yapısal özellikleri üzerinde yararlı etkileri olduğu ortaya konmuştur. Ek olarak DFI açısından zararlı bir etkisi olmadığı gözlemlenmiştir. Bu veriler değerlendirildiğinde borun (sodyum pentaborat) alternatif bir sperma sulandırıcısı bileşeni olarak kullanılabileceği sonucuna ulaşılmıştır
References
- Ahmad M, Ahmad N, Riaz A, et al. (2015): Sperm survival kinetics in different types of bull semen: Progressive acrosomal status and reactive oxygen species generation. Reprod Fert Develop, 27, 784-793. membrane integrity,
- Almadaly E, Farrag F, Shukry M, et al. (2014): Plasma membrane integrity and morphology of frozen-thawed bull spermatozoa supplemented with desalted and lyophilized seminal plasma. Glob Vet, 13, 753-766.
- Amirat L, Tainturier D, Jeanneau L, et al. (2004): Bull semen in vitro fertility after cryopreservation using egg yolk LDL: A comparison with Optidyl®, a commercial egg yolk extender. Theriogenology, 61, 895-907. 4. Anzar M, Kroetsch T, Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Anim Reprod Sci, 126, 23-31. L (2011):
- Bakırdere S, Örenay S, Korkmaz M (2010): Effect of boron on human health. TOMPJ, 3, 54-59.
- Başaran N, Duydu Y, Bolt MH (2012): Reproductive toxicity in boron exposed workers in Bandırma, Turkey. J Trace Elem Med BioI, 26, 165-167.
- Bochenek M, Smorag Z, Pilch J (2001): Sperm chromatin structure assay of bulls qualified for artificial insemination. Theriogenology, 56, 557-567.
- D’Occhio MJ, Hengstberger KJ, Johnston SD (2007): Biology of sperm chromatin structure and relationship to male fertility and embryonic survival. Anim Reprod Sci, 101, 1-17.
- Demirtaş A (2006): Bor bileşikleri ve tarımda kullanımı. Atatürk Univ J Agricultural Faculty, 37, 111-115.
- Donnelly ET, Lewis SE, McNally JA, et al. (1998): In vitro fertilization and pregnancy rates: The influence of sperm motility and morphology on IVF outcome. Fertil Steril, 70, 305-314.
- Duydu Y, Başaran N, Üstündağ A, et al. (2011): Reproductive toxicity parameters and biological monitoring in occupationally and environmentally boron-exposed persons in Bandırma, Turkey. Arch Toxicol, 85, 589-600.
- Eti Mine Works General Directorate (2016): World Boron http://etimaden.gov.tr/tr/page/uretim-dunya-bor-rezervleri. (Accessed Feb 09, 2016). Available at
- Evenson D, Jost L (2001): Sperm chromatin structure assay for fertility assessment. 1-7. In: JP Robinson (Ed), Current protocols in cytometry. John Wiley & Sons, Inc., New York.
- Fetterolf PM, Rogers BJ (1990): Prediction of human sperm penetrating ability using computerized motion parameters. Mol Reprod Dev, 27, 326-331.
- Fleischa A, Malamaa E, Witschib U, et al. (2017): Effects of an extension of the equilibration period up to 96 hours on the Theriogenology, 89, 255-262. cryopreserved bull semen.
- Holt WV (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci, 62, 3-22.
- Karabinus DS, Evenson DP, Jost LK, et al. (1990): Comparison of semen quality in young and mature Holstein bulls measured by light microscopy and flow cytometry. J Dairy Sci, 73, 2364-2371.
- Karoui S, Díaz C, González-Marín C, et al. (2012): Is sperm DNA fragmentation a good marker for field AI bull fertility? J Anim Sci, 90, 2437-2449.
- Korkmaz M, Saylı U, Saylı SB, et al. (2007): Estimation of human daily boron exposure in a boron-rich area. Brit J Nutr, 98, 571-575.
- Korkmaz M, Yenigün M, Bakırdere S, et al. (2011): Effects of chronic boron exposure on semen profile. Biol Trace Elem Res, 143, 738-750.
- Ku WW, Chapin RE, Wine RN, et al. (1993): Testicular toxicity of boric acid: relationship of dose to lesion development and lack of recovery in the F344 rat. Reprod Toxicol, 7, 305-319.
- Lemma A (2010): Effect of cryopreservation on sperm quality and fertility. 191-216. In: M Manafi (Ed), Artificial insemination in farm animals. INTECH Open Access Publisher.
- Liu DY, Clarke GN, Baker HG (1991): Relationship between sperm motility assessed with the Hamilton‐Thorn motility analyzer and fertilization rates in vitro. J Androl, 12, 231-239.
- Moallem U, Neta N, Zeron Y, et al. (2015): Dietary a- linolenic acid from flaxseed oil or eicosapentaenoic and docosahexaenoic acids from fish oil differentially alter fatty acid composition and characteristics of fresh and frozen- thawed bull semen. Theriogenology, 30, 1-11.
- Nagy S, Jansen J, Topper EK, et al. (2003): A triple-stain flow cytometric method to assess plasma-and acrosome- membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod, 68, 1828-1835.
- Saylı BS, Tüccar E, Elhan AH (1998): An assessment of fertility in boron-exposed Turkish subpopulations. Reprod Toxicol, 12, 297-304.
- Sukcharoen N, Keith J, Irvine DS, et al. (1996): Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: the effect of the delay between testing and IVF. Hum Reprod, 11, 1030- 1034.
- Sukcharoen N, Sithipravej T, Promviengchai S, et al. (1998): Sperm morphology evaluated by computer (IVOS) cannot predict the fertilization rate in vitro after intracytoplasmic sperm injection. Fertil Steril, 69, 564-568.
- Tırpan MB, Tekin N (2015): Effects of boron (sodium pentaborate components, on freezing and post-thaw quality of Angora buck semen. Vet J Ankara Univ, 62, 295-302. of Tris
- Treinen KA, Chapin RE (1991): Development of testicular lesions in f344 rats after treatment with boric acid. Toxicol Appl Pharm, 107, 325-335.
- Türkez H, Geyikoğlu F, Tatar A, et al. (2007): Effects of some boron compounds on peripheral human blood. Z Naturforsch, 62, 889-896.
- Watson PF (2000): The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci, 60, 481-492.
Abstract
In this research, post-thaw effects of bull semen extenders including different amounts of boron (sodium pentaborate) on total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI) were evaluated. Eight ejaculates were examined from four different bulls. Each ejaculate was split in to five aliquots and extended with standard Tris extender (Control) and 0.4 g boron with 0.5 g glucose (Group 1) 0.5; 0.7; 0.9 g boron without glucose (Group 2, Group 3 and Group 4 respectively) were used as experimental groups. After thawing TMOT, PMOT, kinetic traits of spermatozoa, PMAI and DFI were determined. According to motility and progressive motility (66.04%, 50.79%), Group 1 had higher values than the others, but differences between groups were not statistically significant (P>0.05). The highest values of VSL, LIN, STR and WOB were determined (28.15 µm/s, 42.98%, 71.69%, 59.71%) in Group 1 (P<0.05). While other kinetic traits of spermatozoa were higher numerically in Group 1 than the other groups, differences between groups were not statistically significant (P>0.05). The highest PMAI value was determined as 60.84% in Group 1 (P>0.05). The DFI rates were found similar in all control and experimental groups (P>0.05). In conclusion, it was observed that the boron (sodium pentaborate) has positive effects on post-thaw movement and structural traits. In addition, boron has no detrimental effect on DNA fragmentation index in semen freezing. Thus, boron could be used as an alternative semen extender compound
References
- Ahmad M, Ahmad N, Riaz A, et al. (2015): Sperm survival kinetics in different types of bull semen: Progressive acrosomal status and reactive oxygen species generation. Reprod Fert Develop, 27, 784-793. membrane integrity,
- Almadaly E, Farrag F, Shukry M, et al. (2014): Plasma membrane integrity and morphology of frozen-thawed bull spermatozoa supplemented with desalted and lyophilized seminal plasma. Glob Vet, 13, 753-766.
- Amirat L, Tainturier D, Jeanneau L, et al. (2004): Bull semen in vitro fertility after cryopreservation using egg yolk LDL: A comparison with Optidyl®, a commercial egg yolk extender. Theriogenology, 61, 895-907. 4. Anzar M, Kroetsch T, Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Anim Reprod Sci, 126, 23-31. L (2011):
- Bakırdere S, Örenay S, Korkmaz M (2010): Effect of boron on human health. TOMPJ, 3, 54-59.
- Başaran N, Duydu Y, Bolt MH (2012): Reproductive toxicity in boron exposed workers in Bandırma, Turkey. J Trace Elem Med BioI, 26, 165-167.
- Bochenek M, Smorag Z, Pilch J (2001): Sperm chromatin structure assay of bulls qualified for artificial insemination. Theriogenology, 56, 557-567.
- D’Occhio MJ, Hengstberger KJ, Johnston SD (2007): Biology of sperm chromatin structure and relationship to male fertility and embryonic survival. Anim Reprod Sci, 101, 1-17.
- Demirtaş A (2006): Bor bileşikleri ve tarımda kullanımı. Atatürk Univ J Agricultural Faculty, 37, 111-115.
- Donnelly ET, Lewis SE, McNally JA, et al. (1998): In vitro fertilization and pregnancy rates: The influence of sperm motility and morphology on IVF outcome. Fertil Steril, 70, 305-314.
- Duydu Y, Başaran N, Üstündağ A, et al. (2011): Reproductive toxicity parameters and biological monitoring in occupationally and environmentally boron-exposed persons in Bandırma, Turkey. Arch Toxicol, 85, 589-600.
- Eti Mine Works General Directorate (2016): World Boron http://etimaden.gov.tr/tr/page/uretim-dunya-bor-rezervleri. (Accessed Feb 09, 2016). Available at
- Evenson D, Jost L (2001): Sperm chromatin structure assay for fertility assessment. 1-7. In: JP Robinson (Ed), Current protocols in cytometry. John Wiley & Sons, Inc., New York.
- Fetterolf PM, Rogers BJ (1990): Prediction of human sperm penetrating ability using computerized motion parameters. Mol Reprod Dev, 27, 326-331.
- Fleischa A, Malamaa E, Witschib U, et al. (2017): Effects of an extension of the equilibration period up to 96 hours on the Theriogenology, 89, 255-262. cryopreserved bull semen.
- Holt WV (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci, 62, 3-22.
- Karabinus DS, Evenson DP, Jost LK, et al. (1990): Comparison of semen quality in young and mature Holstein bulls measured by light microscopy and flow cytometry. J Dairy Sci, 73, 2364-2371.
- Karoui S, Díaz C, González-Marín C, et al. (2012): Is sperm DNA fragmentation a good marker for field AI bull fertility? J Anim Sci, 90, 2437-2449.
- Korkmaz M, Saylı U, Saylı SB, et al. (2007): Estimation of human daily boron exposure in a boron-rich area. Brit J Nutr, 98, 571-575.
- Korkmaz M, Yenigün M, Bakırdere S, et al. (2011): Effects of chronic boron exposure on semen profile. Biol Trace Elem Res, 143, 738-750.
- Ku WW, Chapin RE, Wine RN, et al. (1993): Testicular toxicity of boric acid: relationship of dose to lesion development and lack of recovery in the F344 rat. Reprod Toxicol, 7, 305-319.
- Lemma A (2010): Effect of cryopreservation on sperm quality and fertility. 191-216. In: M Manafi (Ed), Artificial insemination in farm animals. INTECH Open Access Publisher.
- Liu DY, Clarke GN, Baker HG (1991): Relationship between sperm motility assessed with the Hamilton‐Thorn motility analyzer and fertilization rates in vitro. J Androl, 12, 231-239.
- Moallem U, Neta N, Zeron Y, et al. (2015): Dietary a- linolenic acid from flaxseed oil or eicosapentaenoic and docosahexaenoic acids from fish oil differentially alter fatty acid composition and characteristics of fresh and frozen- thawed bull semen. Theriogenology, 30, 1-11.
- Nagy S, Jansen J, Topper EK, et al. (2003): A triple-stain flow cytometric method to assess plasma-and acrosome- membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod, 68, 1828-1835.
- Saylı BS, Tüccar E, Elhan AH (1998): An assessment of fertility in boron-exposed Turkish subpopulations. Reprod Toxicol, 12, 297-304.
- Sukcharoen N, Keith J, Irvine DS, et al. (1996): Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: the effect of the delay between testing and IVF. Hum Reprod, 11, 1030- 1034.
- Sukcharoen N, Sithipravej T, Promviengchai S, et al. (1998): Sperm morphology evaluated by computer (IVOS) cannot predict the fertilization rate in vitro after intracytoplasmic sperm injection. Fertil Steril, 69, 564-568.
- Tırpan MB, Tekin N (2015): Effects of boron (sodium pentaborate components, on freezing and post-thaw quality of Angora buck semen. Vet J Ankara Univ, 62, 295-302. of Tris
- Treinen KA, Chapin RE (1991): Development of testicular lesions in f344 rats after treatment with boric acid. Toxicol Appl Pharm, 107, 325-335.
- Türkez H, Geyikoğlu F, Tatar A, et al. (2007): Effects of some boron compounds on peripheral human blood. Z Naturforsch, 62, 889-896.
- Watson PF (2000): The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci, 60, 481-492.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA72DY87BV |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2018 |
| Published in Issue | Year 2018 |