Letter to Editor
Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks
Abstract
In this study, we have investigated the seroprevalence of alphaherpesvirus (BoHV-1 and CpHV-1) infections in selected goat flocks using glycoprotein B (gB)/glycoprotein E (gE) ELISA combination and virus neutralisation test (VNT). For this purpose, we collected blood serum samples of 546 Saanen goats from Bolu province and tested them by ELISA and VNT. Using ELISA, 32.05% (175/546) of the samples were found to be gB(+)/gE(-) for CpHV-1, whereas only 0.73% (4/546) of the samples were noted to be gB(+)/gE(+) for BoHV-1 releated infection. By performing VNT, we found 31.86% (174/546) and 3.29% (18/546) positivity for CpHV-1 and BoHV-1, respectively. In conclusion, prevalence of CpHV-1 infection was found to be higher than BoHV1 infection in sampled goat flocks. For diagnosis of alphaherpesviruses in the goats, use of gB blocking ELISA test was found to be favorable as an alternative method. On the other hand, to distinguish between the CpHV-1/BoHV-1, gE blocking ELISA in combination by VNT was found to be incompatible with the statistical analysis (p<0.001). Considering antigenic cross-reactions between these viruses, because of the incompatibility of these tests, using a more sensitive/specific method to determine CpHV-1 and BoHV-1 antibodies can be suggested
References
- Alpay G, Tuncer P, Yesilbag K (2014): Serological distribution of some viral infections in cattle, sheep and goats in an isolated islandecosystem. Vet J Ankara Univ, 61, 43-48.
- Ataseven VS, Basaran Z, Yılmaz V, et al. (2010): Seroprevalence of parainfluenza virus-3 (PIV-3) and bovine herpesvirus type 1 (BHV-1) infections in goats of Van region. YYÜ Veteriner Fak Derg, 21, 7-9.
- Berrios PE, Mckercher DG (1975): Characterization caprine herpesvirus. Am J Vet Res, 36, 1755-1762.
- Bertolotti L, Rosamilia A, Profiti M, et al. (2013): Characterization of caprine herpesvirus 1 (CpHV1) glycoprotein E ve glycoprotein I ectodomains expressed in mammalian cells. Vet Microbiol, 164, 222-228.
- El-Azhary MA, Roy RS, Frechette JL (1979): Serological evidence of IBR and BVD infection in Caribou (Rangifer tarandus). Vet Rec, 105, 336.
- Frey HR, Liess B (1971): Vermehrungskinetik und Verwendbarkeit einer stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der mikrotiter-Methode. Zentbl Vet Med, 18, 61-71.
- Fulton RW, Briggs RE, Payton ME, et al. (2004): Maternally derived humoral immunity to bovine viral diarrhea virus (BVDV) 1a, BVDV1b, BVDV2, bovine herpesvirus-1, parainfluenza-3 virus bovine respiratory syncytial virus, Mannheimia haemolytica and Pasteurella multocida in beef calves, antibody decline by half-life studies and effect on response to vaccination. Vaccine, 22, 643-649.
- Guercio A, Greco G, Lanizzoto G, et al. (1998): Valutazione della diffusione di anticorpi anti Herpes Virus della capra in allevamenti caprini della Sicilia. Atti. SIPAOC, 12, 138-142.
- Keuser V, Espejo-Serrano J, Schynts F, et al. (2004): Isolation of caprine herpesvirus type 1 in Spain. Vet Rec, 154, 395-399.
- Koptopoulos G, Papanastasopoulou M, Papadopoulos O, et al. (1988): The epizootology of caprine herpesvirus (BHV-6) infections in goat populations in Greece. Comp Immunol Microb, 11, 199-205.
- Kramps JA, Banks M, Beer M, et al. (2004): Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe. Vet Microbiol, 102, 169-181.
- Lyaku JR, Nettleton PF, Mardsen H (1992): A comparison of serological relationships among five ruminant alphaherpesviruses by ELISA. Arch Virol, 124, 333-341.
- Mahmoud MA, Ahmed SA (2009): Prevalence of bovine herpesvirus-1 in sheep and goats in Egypt. Lobal Veterinaria Giza, 3, 472-479.
- Marinaro M, Bellacicco AL, Tarsitano E, et al. (2010): Detection of CpHV-1 spesific antibodies in goat sera using an ELISA and SN Test. J Vet Diagn Invest, 22, 245-248.
- Martin WB, Castrucci G, Frigeri F, et al. (1990): A serological comparison of some animal herpesviruses. Comp Immunol Microbiol Infect Dis, 13, 75-84.
- Nixon P, Edwards S, White H (1988): Serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. Vet Res Commun, 12, 355-362.
- Ros C, Belak S (2002): Characterization of the glycoprotein B gene from ruminant alphaherpesviruses. Virus Genes, 24, 99-105.
- Saito JK, Grıbble DH, Berrios PE, et al. (1974): A new herpesvirus isolate from goats: Preliminary report. Am J Vet Res, 35, 847-848.
- Silva MLCR, Pituco EM, Nogueira AHC, et al. (2013): Serological evidence and risk factors associated cith caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil. J Vet Diagn Invest, 25, 125.
- Taylor J. Herbert (1977): Increase in DNA replication sites in cells held at the beginning of S phase. Chromosoma, 62, 291-300.
- Tempesta M, Cavalli A, Voigt V, et al. (1994): Presenza di anticorpi per caprine herpesvirus 1 (CapHV.1) in allevamenti caprini dell’Italia meridionale. Atti. SIPAOC, 11, 121-122.
- Thiry J, Keuser V, Muylkens B, et al. (2006a): Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res, 37, 169-190.
- Thiry J, Tempesta M, Camero M, et al. (2007): Clinical protection against caprine herpesvirus 1 genital infection by intranasal administration of a live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine. BMC Vet Res, 3, 33.
- Thiry J, Tempesta M, Camero M, et al. (2006b): A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats. Vet Microbiol, 113, 303- 308.
- Thiry J, Saegerman C, Chartier C, et al. (2008): Serological evidence of caprine herpesvirus 1 infection in Mediterranean France. Vet Microbiol, 128, 261-268.
- Yeşilbağ K, Bilge Dağalp S, Okur Gümüşova S, et al. (2003): Studies on Herpesvirus Infections of Goats in Turkey: Prevalence of Antibodies to Bovine herpesvirus 1. Rev Med Vet, 154, 772-774.
- Yonguç AD, Akçora A, Vural B (1990): Serological and etiological studies on caprine herpesvirus infection. Project No: Tubitak, VHAG-756. Geliş tarihi: 11.05.2016 / Kabul tarihi: 22.10.2016 Adress for correspondence: Merve Özgür Baydın, Veterinary Control Central Research Institute, Ankara, Turkey. e-mail: merveozgur87@gmail.com
Seçilen keçi sürülerinde gB/gE ELISA kombinasyonu ve VNT kullanılarak BoHV-1 ve CpHV-1enfeksiyonlarının seroprevalansının araştırılması
Abstract
Bu çalışmada, seçilmiş keçi sürülerinde BoHV-1 ve CpHV-1 enfeksiyonlarının seroprevalansı glikoprotein B (gB)/ glikoprotein E (gE) ELISA kombinasyonu ve virus nötralizasyon testi (VNT) kullanılarak araştırılmıştır. Bu amaçla, Bolu ilinden 546 Saanen keçisinden alınan kan serumu örnekleri ELISA ve VNT kullanılarak test edilmiştir. Her iki ELISA sistemi ile test edilen örneklerin %32.05’i (175/546) CpHV-1 antikor pozitifliğini yansıtan gB(+)/gE(-) olarak bulunurken, % 0.73’ü (4/546) BoHV-1 antikorları yönünden pozitifliği yansıtan gB(+)/gE(+) olarak belirlenmiştir. VNT ile serumların %31.86’ sı (174/546) CpHV-1, %3.29’u (18/546) BoHV-1 antikorları yönünden pozitif olarak değerlendirilmiştir. Sonuç olarak, örneklenen keçi sürülerinde CpHV1 enfeksiyonu prevalansı, BoHV-1 enfeksiyonundan yüksek bulunmuştur. Keçilerde alphaherpesvirus enfeksiyonlarının tanısında gB blocking ELISA alternatif test olarak kullanılabillir bulunmuş, ancak CpHV-1 ve BoHV-1 ayrımında gE blocking ELISA ve VNT kombinasyonunun kullanılması istatisitiksel (p<0.001) olarak uygun bulunmamıştır. Bu viruslar arasındaki antijenik çaprazreaksiyonları göz önünde bulundurarak, bu testlerdeki uyumsuzluklardan dolayı, CpHV-1 ve BoHV-1 antikorlarının belirlenmesi amacıyla daha hassas ve spesifik metotların geliştirilmesi önerilmektedir
References
- Alpay G, Tuncer P, Yesilbag K (2014): Serological distribution of some viral infections in cattle, sheep and goats in an isolated islandecosystem. Vet J Ankara Univ, 61, 43-48.
- Ataseven VS, Basaran Z, Yılmaz V, et al. (2010): Seroprevalence of parainfluenza virus-3 (PIV-3) and bovine herpesvirus type 1 (BHV-1) infections in goats of Van region. YYÜ Veteriner Fak Derg, 21, 7-9.
- Berrios PE, Mckercher DG (1975): Characterization caprine herpesvirus. Am J Vet Res, 36, 1755-1762.
- Bertolotti L, Rosamilia A, Profiti M, et al. (2013): Characterization of caprine herpesvirus 1 (CpHV1) glycoprotein E ve glycoprotein I ectodomains expressed in mammalian cells. Vet Microbiol, 164, 222-228.
- El-Azhary MA, Roy RS, Frechette JL (1979): Serological evidence of IBR and BVD infection in Caribou (Rangifer tarandus). Vet Rec, 105, 336.
- Frey HR, Liess B (1971): Vermehrungskinetik und Verwendbarkeit einer stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der mikrotiter-Methode. Zentbl Vet Med, 18, 61-71.
- Fulton RW, Briggs RE, Payton ME, et al. (2004): Maternally derived humoral immunity to bovine viral diarrhea virus (BVDV) 1a, BVDV1b, BVDV2, bovine herpesvirus-1, parainfluenza-3 virus bovine respiratory syncytial virus, Mannheimia haemolytica and Pasteurella multocida in beef calves, antibody decline by half-life studies and effect on response to vaccination. Vaccine, 22, 643-649.
- Guercio A, Greco G, Lanizzoto G, et al. (1998): Valutazione della diffusione di anticorpi anti Herpes Virus della capra in allevamenti caprini della Sicilia. Atti. SIPAOC, 12, 138-142.
- Keuser V, Espejo-Serrano J, Schynts F, et al. (2004): Isolation of caprine herpesvirus type 1 in Spain. Vet Rec, 154, 395-399.
- Koptopoulos G, Papanastasopoulou M, Papadopoulos O, et al. (1988): The epizootology of caprine herpesvirus (BHV-6) infections in goat populations in Greece. Comp Immunol Microb, 11, 199-205.
- Kramps JA, Banks M, Beer M, et al. (2004): Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe. Vet Microbiol, 102, 169-181.
- Lyaku JR, Nettleton PF, Mardsen H (1992): A comparison of serological relationships among five ruminant alphaherpesviruses by ELISA. Arch Virol, 124, 333-341.
- Mahmoud MA, Ahmed SA (2009): Prevalence of bovine herpesvirus-1 in sheep and goats in Egypt. Lobal Veterinaria Giza, 3, 472-479.
- Marinaro M, Bellacicco AL, Tarsitano E, et al. (2010): Detection of CpHV-1 spesific antibodies in goat sera using an ELISA and SN Test. J Vet Diagn Invest, 22, 245-248.
- Martin WB, Castrucci G, Frigeri F, et al. (1990): A serological comparison of some animal herpesviruses. Comp Immunol Microbiol Infect Dis, 13, 75-84.
- Nixon P, Edwards S, White H (1988): Serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. Vet Res Commun, 12, 355-362.
- Ros C, Belak S (2002): Characterization of the glycoprotein B gene from ruminant alphaherpesviruses. Virus Genes, 24, 99-105.
- Saito JK, Grıbble DH, Berrios PE, et al. (1974): A new herpesvirus isolate from goats: Preliminary report. Am J Vet Res, 35, 847-848.
- Silva MLCR, Pituco EM, Nogueira AHC, et al. (2013): Serological evidence and risk factors associated cith caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil. J Vet Diagn Invest, 25, 125.
- Taylor J. Herbert (1977): Increase in DNA replication sites in cells held at the beginning of S phase. Chromosoma, 62, 291-300.
- Tempesta M, Cavalli A, Voigt V, et al. (1994): Presenza di anticorpi per caprine herpesvirus 1 (CapHV.1) in allevamenti caprini dell’Italia meridionale. Atti. SIPAOC, 11, 121-122.
- Thiry J, Keuser V, Muylkens B, et al. (2006a): Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res, 37, 169-190.
- Thiry J, Tempesta M, Camero M, et al. (2007): Clinical protection against caprine herpesvirus 1 genital infection by intranasal administration of a live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine. BMC Vet Res, 3, 33.
- Thiry J, Tempesta M, Camero M, et al. (2006b): A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats. Vet Microbiol, 113, 303- 308.
- Thiry J, Saegerman C, Chartier C, et al. (2008): Serological evidence of caprine herpesvirus 1 infection in Mediterranean France. Vet Microbiol, 128, 261-268.
- Yeşilbağ K, Bilge Dağalp S, Okur Gümüşova S, et al. (2003): Studies on Herpesvirus Infections of Goats in Turkey: Prevalence of Antibodies to Bovine herpesvirus 1. Rev Med Vet, 154, 772-774.
- Yonguç AD, Akçora A, Vural B (1990): Serological and etiological studies on caprine herpesvirus infection. Project No: Tubitak, VHAG-756. Geliş tarihi: 11.05.2016 / Kabul tarihi: 22.10.2016 Adress for correspondence: Merve Özgür Baydın, Veterinary Control Central Research Institute, Ankara, Turkey. e-mail: merveozgur87@gmail.com
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA47DT27NT |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2017 |
| Published in Issue | Year 2017 |