In vivo and in vitro assessment of ovarian echotexture through computer assisted real time ultrasonography in bitches
Abstract
In vivo and in vitro assessment of canine ovaries (n=72) were studied by computer assisted real time ultrasonography to analyse the correlation between the ovarian echotexture and serum progesterone (P4) concentration. Pre- and postoperative ovarian images were taken via B-mode ultrasonography, and analysed by an image analysis software package for total area of ovary (A), mean greyness value (MGV), heterogeneity (H), and minimum-maximum pixel value (PR). The ovaries were classified into follicular or luteal stage based upon the serum P4 concentration, vaginal cytology findings, and macroscopic inspection of ovaries postoperatively. The P4 was negatively (P<0.05) correlated with A, H and PR, whereas, it’s correlation with MGV was positive (P<0.05) in follicular stage. Also, parameters A, H and PR were higher (P<0.05) in follicular stage. In vitro parameters of MGV, H and PR were higher (P<0.05) than in vivo parameters in follicular stage. Meanwhile, in luteal stage ovaries, only in vitro MGV was higher than in vivo MGV. In this study, it was observed that the relationship between ovarian echotexture and oestrous stages could be presented numerically. Moreover, the differences between the in vivo and in vitro ovarian images were more prominent in bitches in the follicular stage
References
- Alacam E, Ay SS, Saban E (2009): The evaluation of various radioimmunoassay progesterone kits for different stage of reproductive period in the cow, sheep and bitch. Vet J Ankara Univ, 56, 37-41.
- Bergeron LH, Nykamph SG, Brisson BA, et al. (2013): B-mode ultrasonography for detecting periovulatory events in the bitch. Theriogenology, 79, 274-283. color Doppler
- Davies KL, Bartlewski PM, Pierson RA, et al. (2006): Computer assisted image analyses of corpora lutea in relation to peripheral concentrations of progesterone: A comparison between breeds of sheep with different ovulation rates. Anim Reprod Sci, 96, 165-175.
- Gastal EL, Gastal MO, Ginther OJ (1998): The suitability of echotexture characteristics of the follicular wall for identifying Theriogenology, 50, 1025-1038. breeding day in mare.
- Gastal EL, Gastal MO, Ginther OJ (2006): Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effect of human chorionic gonadotropin in mares. Reproduction, 131, 699- 709.
- Herzog K, Kiossis E, Bollwein H (2008): Examination of cyclic changes in bovine luteal echotexture using computer– assisted statistical pattern recognition techniques. Anim Reprod Sci, 106, 289-297.
- Kayacık V, Salmanoğlu MR, Polat B, et al. (2005): Evaluation of the corpus luteum size throughout the cycle by ultrasonography and progesterone assay in cows. Turk J Vet Anim Sci, 29, 1311-1316.
- Liu X, Hart EJ, Petrik JJ, et al. (2008): Relationships between histomorphology and proliferating cell nuclear antigen expression of bovine antral follicles and corpora lutea ex situ. Reprod Dom Anim, 43, 27-34. image attributes,
- Olson PN, Bowen RA, Behrendt MD, et al. (1982): Concentration of reproductive hormones in canine serum throughout late anestrus, proestrus and estrus. Biol Reprod, 27, 1196-1206.
- Pierson RA, Adams GP (1995): Computer-assisted image analysis, diagnostic ultrasonography and ovulation induction: Strange bedfellows. Theriogenology, 43, 105- 112.
- Rasband WS (1997): Image J. U.S. National Institutes of Health. Bethesda, Maryland, USA. http:rsb.info.nih.gov/ij., 1997.
- Singh J, Pierson RA, Adams GP (1997): Ultrasound image attributes of the bovine corpus luteum: Structural and functional correlates. J Reprod Fertil, 109, 35-44.
- Singh J, Pierson RA, Adams GP (1998): Ultrasound image attributes of bovine ovarian follicles and endocrine and functional correlates. J Reprod Fertil, 112, 19-29.
- Singh J, Pierson RA, Adams GP (2003): Promise of new imaging technologies for assessing ovarian function. Anim Reprod Sci, 78, 371-399.
- Siqueira LGB, Torres CAA, Amorim LS, et al. (2009): Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Anim Reprod Sci, 115, 18-28.
- Thijssen JM, Herzog K, Weijers G, et al. (2011): Ultrasound image analysis offers the opportunity to predict plasma progesterone concentrations in the estrous cycle in cows: A feasibility study. Anim Reprod Sci, 127, 7-15.
- Tom JW, Pierson RA, Adams GP (1998): Quantitative of echotexture Theriogenology, 49, 1345-1352. bovine corpora lutea.
- Wu D, Barret DMW, Rawlings NC, et al. (2009): Relationship of changes in ultrasonographic image attributes to ovulatory and steroidogenic capacity of large antral follicles in sheep. Anim Reprod Sci, 116, 73-84.
- Zagzebski JA (1996): Essential of Ultrasound Physics. Mosby-Year Book Inc, St. Louis, USA.
Köpeklerde ovaryum ekotekstürünün bilgisayar destekli B - mod ultrasonografi ile in vivo ve in vitro incelenmesi
Abstract
Köpeklerde ovaryum ekotekstürü ile serum progesteron (P4) değerleri arasındaki ilişkinin belirlenmesi için köpek ovaryumları (n=72) in vivo ve in vitro olarak bilgisayar destekli real time ultrasonografi ile incelendi. Total ovaryum alanı (A), ortalama grilik değeri (MGV), heterojenite (H) ve minimum-maksimum piksel değerinin (PR) görüntü analiz yazılım sistemi ile analiz edilmesi için preoperatif ve postoperatif ovaryum B-mod ultrasonografi görüntüleri alındı. Ovaryumlar, serum P4 değerleri, vajinal sitoloji bulguları ve postoperatif makroskobik görünümlerine göre folliküler ve luteal evre olarak sınıflandırıldı. Folliküler evre grubunda P4 değeri A, H ve PR ile negatif korelasyon gösterirken (P<0.05), MGV ile pozitif korelasyon gösterdi (P<0.05). Yine folliküler evrede A, H ve PR değerleri önemli ölçüde yüksekti (P<0.05). Folliküler evrede in vitro görüntülerindeki MGV, H ve PR değerleri in vivo görüntülerdekinden daha yüksek bulundu (P<0.05). Ancak, luteal evredeki ovaryumlarda, sadece MGV in vitro görüntülerde daha yüksekti. Bu çalışmada, ovaryum ekotekstürü ile östrus dönemleri arasında sayısal olarak sunulabilen bir ilişki olduğu ve folliküler evredeki köpeklerde in vivo ve in vitro görüntüler arasında daha belirgin bir fark bulunduğu görüldü
References
- Alacam E, Ay SS, Saban E (2009): The evaluation of various radioimmunoassay progesterone kits for different stage of reproductive period in the cow, sheep and bitch. Vet J Ankara Univ, 56, 37-41.
- Bergeron LH, Nykamph SG, Brisson BA, et al. (2013): B-mode ultrasonography for detecting periovulatory events in the bitch. Theriogenology, 79, 274-283. color Doppler
- Davies KL, Bartlewski PM, Pierson RA, et al. (2006): Computer assisted image analyses of corpora lutea in relation to peripheral concentrations of progesterone: A comparison between breeds of sheep with different ovulation rates. Anim Reprod Sci, 96, 165-175.
- Gastal EL, Gastal MO, Ginther OJ (1998): The suitability of echotexture characteristics of the follicular wall for identifying Theriogenology, 50, 1025-1038. breeding day in mare.
- Gastal EL, Gastal MO, Ginther OJ (2006): Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effect of human chorionic gonadotropin in mares. Reproduction, 131, 699- 709.
- Herzog K, Kiossis E, Bollwein H (2008): Examination of cyclic changes in bovine luteal echotexture using computer– assisted statistical pattern recognition techniques. Anim Reprod Sci, 106, 289-297.
- Kayacık V, Salmanoğlu MR, Polat B, et al. (2005): Evaluation of the corpus luteum size throughout the cycle by ultrasonography and progesterone assay in cows. Turk J Vet Anim Sci, 29, 1311-1316.
- Liu X, Hart EJ, Petrik JJ, et al. (2008): Relationships between histomorphology and proliferating cell nuclear antigen expression of bovine antral follicles and corpora lutea ex situ. Reprod Dom Anim, 43, 27-34. image attributes,
- Olson PN, Bowen RA, Behrendt MD, et al. (1982): Concentration of reproductive hormones in canine serum throughout late anestrus, proestrus and estrus. Biol Reprod, 27, 1196-1206.
- Pierson RA, Adams GP (1995): Computer-assisted image analysis, diagnostic ultrasonography and ovulation induction: Strange bedfellows. Theriogenology, 43, 105- 112.
- Rasband WS (1997): Image J. U.S. National Institutes of Health. Bethesda, Maryland, USA. http:rsb.info.nih.gov/ij., 1997.
- Singh J, Pierson RA, Adams GP (1997): Ultrasound image attributes of the bovine corpus luteum: Structural and functional correlates. J Reprod Fertil, 109, 35-44.
- Singh J, Pierson RA, Adams GP (1998): Ultrasound image attributes of bovine ovarian follicles and endocrine and functional correlates. J Reprod Fertil, 112, 19-29.
- Singh J, Pierson RA, Adams GP (2003): Promise of new imaging technologies for assessing ovarian function. Anim Reprod Sci, 78, 371-399.
- Siqueira LGB, Torres CAA, Amorim LS, et al. (2009): Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Anim Reprod Sci, 115, 18-28.
- Thijssen JM, Herzog K, Weijers G, et al. (2011): Ultrasound image analysis offers the opportunity to predict plasma progesterone concentrations in the estrous cycle in cows: A feasibility study. Anim Reprod Sci, 127, 7-15.
- Tom JW, Pierson RA, Adams GP (1998): Quantitative of echotexture Theriogenology, 49, 1345-1352. bovine corpora lutea.
- Wu D, Barret DMW, Rawlings NC, et al. (2009): Relationship of changes in ultrasonographic image attributes to ovulatory and steroidogenic capacity of large antral follicles in sheep. Anim Reprod Sci, 116, 73-84.
- Zagzebski JA (1996): Essential of Ultrasound Physics. Mosby-Year Book Inc, St. Louis, USA.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA38VS94RZ |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2017 |
| Published in Issue | Year 2017 |