Letter to Editor
Year 2016,
, 371 - 376, 01.12.2016
Abstract
In this study, it was aimed to interpret the effects of hypothyroidism and hyperthyroidism on hematological parameters in rats. A total of 42 male Wistar rats were divided into 3 equally groups as euthyroid, hypothyroid, hyperthyroid. Hypothyroidism and hyperthyroidism were induced by 6-n-propyl-2-thiouracil (PTU; 0,05%, w/v) and L-thyroxine (L-T4; 0,0012%, w/v) received in drinking water, respectively. Blood samples were withdrawn from 7 rats of each group at the end of 3rd and 6th weeks for determination of the hematological parameters and serum hormone profiles. Among the 3rd week groups only percentage of monocyte was increased (p<0.05) in the hypothyroid group when compared to the euthyroid group. In the 6th week hypothyroid group, red blood cell (RBC) counts, hemoglobin (Hb) concentration, hematocrit (Hct) value and percentage (%) of red cell distribution width (RDW) were lower (p<0.05) than the 6th week euthyroid and hyperthyroid groups. However, plateletcrit (PCT) % was higher (p<0.05) in the 6th week hypothyroid group compared to the 6th week euthyroid group. In the 6th week hyperthyroid group, mean corpuscular hemoglobin concentration (MCHC) and platelet (PLT) counts were lower (p<0.05) than the 6th week hypothyroid group. In the 6th week hyperthyroid group percentage of lymphocyte was decreased (p<0.05) and percentage of granulocyte was increased (p<0.05) when compared to the 6th week euthyroid group. In conclusion, elongation of the treatment duration with PTU and L-T4 could lead to changes on some hematological parameters. However, these values remained within physiological ranges
References
- Abou-auda HS, Abou-shaaban RRA (2006): Effect of abouthiouline a novel drug with therapeutic potential as antithyroid, on some biochemical and hematologic parameters in mice and rats. Saudi Pharm J, 14, 34-41.
- Bianco AC, Anderson G, Forrest D, et al. (2014): American Thyroid Association Guide to investigating thyroid hormone economy and action in rodent and cell models. Thyroid, 24, 88-168.
- Chandel RS, Gangadhar C, Leela A (2015): Impact of subclinical hematological parameters. APALM, 2, 21-25. on iron status and
- Das K, Chainy GBN (2004): Thyroid hormone influences antioxidant defense system in adult rat brain. Neurochem Res, 29, 1755-1766.
- Davis PJ, Leonard JL, Davis FB (2008): Mechanisms of nongenomic Neuroendocrinol, 29, 211-218. thyroid hormone. Front 6. Dixon RM, Reid SW, Mooney CT (1999): Epidemiological, clinical, haematological and biochemical characteristics of canine hypothyroidism. Vet Rec, 145, 481-487.
- Dönmez N, Keskin E (2009): The effects of aflatoxin and glucomannan on coagulation parameters in rabbits. Veterinarski Arhiv, 79, 555-560.
- El-Bakry AM, El-Gareib AW, Ahmed RG (2010): Comparative study of the effects of experimentally induced hypothyroidism and hyperthyroidism in some brain regions in albino rats. Int J Dev Neurosci, 28, 371-389.
- Fandrey J, Pagel H, Frede S, et al. (1994): Thyroid hormones production in vitro. Exp Hematol, 22, 272-277. erythropoietin
- Ferreira E, Silva AE, Serakides R, et al. (2007): Model of induction of thyroid dysfunctions in adult female mice. Arq Bras Med Vet Zootec, 59, 1245-1249.
- Flamant F, Baxter JD, Forrest D, et al. (2006): International pharmacology and classification of the nuclear receptor superfamily: Thyroid hormone receptors. Pharmacol Rev, 58, 705-711. Pharmacology. LIX. The
- Giknis MLA, Clifford CB (2008): Clinical Laboratory Parameters for Crl:Wl (Han). http://www.criver.com/files/pdfs/rms/wistarhan/rm_rm_r_ wistar_han_clin_lab_parameters_08.aspx.(20.03.2015)
- Golde D W, Bersch N, Chopra IJ, et al. (1977): Thyroid hormones stimulate erythropoiesis in vitro. Br J Haematol, 37, 173-177.
- Guerrero A, Pamplona R, Portero-Otin M, et al. (1999): Effect of thyroid status on lipid composition and peroxidation in the mouse liver. Free Radic Biol Med, 26, 73-80.
- Guyton AC, Hall JE (2011): Thyroid Metabolic Hormones. 931-943. In: Guyton and Hall Textbook of Medical Physiology, Elsevier, United States of America,.
- Hulbert AJ (2000): Thyroid hormones and their effects: A new perspective. Biological Reviews, 75, 519-631.
- Jawad KA, Atyaf JHA-Z (2015): The therapeutic role of alcoholic extract of fenugreek seeds on hypothyroidism state induced by thiourea and some blood parameters in adult male rabbits. The Iraqi J Vet Med, 39, 1-7.
- Kampfmann I, Bauer N, Johannes S, et al. (2012): Differences in hematologic variables in rats of the same strain but different origin. Vet Clin Pathol, 41, 228-234.
- Kawa MP, Grymula K, Paczkowska E, et al. (2010): Clinical relevance of thyroid dysfunction in human haematopoiesis: biochemical and molecular studies. Eur J Endocrinol, 162, 295-305.
- Kendrick TS, Payne CJ, Epis MR, et al. (2008): Erythroid defects in TRalpha-/- mice. Blood, 111, 3245- 3248.
- Klecha AJ, Genaro AM, Gorelik G, et al. (2006): Integrative immune system interaction: Thyroid hormone-mediated modulation of lymphocyte activity through the protein kinase C signaling pathway. J Endocrinol, 189, 45-55.
- Malgor LA, Blanc CC, Klainer E et al. (1975): Direct effects of thyroid hormones on bone marrow erythroid cells of rats. Blood, 45, 671-679.
- Manna D, Roy G, Mugesh G (2013): Antithyroid drugs and their analogues: Synthesis, structure, and mechanism of action. Accounts of Chemical Research, 46, 2706-2715.
- Marongiu F, Barcellona D, Mameli A et al. (2011): Thyroid disorders and hypocoagulability. Semin Thromb Hemost, 37, 11-16.
- Messarah M, Saoudi M, Boumendjel A, et al. (2011): Oxidative stress induced by thyroid dysfunction in rat erythrocytes and heart. Environ Toxicol Pharmacol, 31, 33-41.
- Moriyama K, Tagami T, Usui T, et al. (2007): Antithyroid drugs inhibit thyroid hormone receptor- mediated transcription. J Clin Endocrinol Metab, 92, 1066-1072.
- Mostaghni K, Bashari Maafi A, Badiei K (2005): Study of the effects of experimental hypothyroidism on clinical, hematological and serum biochemical factors in pregnant ewes. Iran J Vet Res, 6, 1-5, 73.
- Nambiar PR, Palanisamy GS, Okerberg C, et al. (2014): Toxicities associated with 1-month treatment with propylthiouracil (PTU) and methimazole (MMI) in male rats. Toxicol Pathol, 42, 970-983.
- Nejar Bruno A, Pochmann D, Klein Ricachenevsky F, et al. (2005): 5′-nucleotidase activity is altered by hypo- and hyperthyroidism in platelets from adult rats. Platelets, 16, 25-30.
- Noyan A (2011): Tiroid Bezi ve Hormonu. 1007-1033. In: Yaşamda ve Hekimlikte Fizyoloji, Palme Yayınları, Ankara.
- Oetting A, Yen PM (2007): New insights into thyroid hormone action. Best Pract Res Clin Endocrinol Metab, 21, 193-208.
- Osonuga IO, Olowookorun MO, Akinola BO (2014): Effects of thyroxine on blood parameters and liver enzymes in adult male and female rats. Afr J Biomed Res, 17, 53-56.
- Serakides R, Ocarino NM, Cardoso TGS, et al. (2005): Response of parathyroid to the plasmatic of calcium and hosphorus hypogonadism in the rat. Arq Bras Med Vet Zootec,
- 57, 48-54. in hyperthyroidism and Sullivan PS, McDonald TP (1992): Thyroxine suppresses thrombocytopoiesis and stimulates erythropoiesis in mice. Proc Soc Exp Biol Med, 201, 271-277.
- Umezu M, Kagabu S, Jiang J, et al. (1998): Evaluation and characterization of congenital hypothyroidism in rdw dwarf rats. Lab Anim Sci, 48, 496-501.
- Vennström B, Liu H, Forrest D (2010): Thyroid hormone receptors. 183-201 In: Chris M. Bunce MJC (Ed) Nuclear Receptors Current Consepts and Future Challenges. Springer, Netherlands.
- Würtz M, Hvas AM, Kristensen SD, et al. (2012): Platelet aggregation is dependent on platelet count in patients with coronary artery disease. Thromb Res, 129, 56-61.
- Yen PM (2001): Physiological and molecular basis of thyroid hormone action. Physiol Rev, 81, 1097-1142.
- Yücel R, Özdemir S, Darıyerli N, et al. (2009): Erythrocyte osmotic fragility and lipid peroxidation in experimental hyperthyroidism. Endocr, 36, 498-502.
- Zahediasl S, Habibi G, Ghasemi A, et al. (2010): Hematological parameters and osmotic fragility of red blood cells in experimentally induced hyperthyroidism in rats. Int J Endocrinol Metab, 8, 74-78.
Year 2016,
, 371 - 376, 01.12.2016
Abstract
Bu çalışmada, hipotiroidizm ve hipertiroidizmin ratların hematolojik parametreleri üzerine etkilerini yorumlamak amaçlanmıştır. Toplam 42 adet erkek Wistar rat, ötiroid, hipotiroid ve hipertiroid olarak 3 eşit gruba ayrıldı. Hipotiroidizm ve hipertiroidizm sırasıyla 6-n-propil-2-tiourasil (PTU; % 0,05, w/v) ve L-tiroksin (T4; % 0,0012, w/v) içme sularına katılarak gerçekleştirildi. Hematolojik parametrelerin ve serum hormon profillerinin belirlenmesi için her grupta 7 rattan, 3. ve 6. haftanın sonunda kan örnekleri toplandı. Üçüncü hafta grupları arasında, yalnızca hipotiroid grubunun monosit yüzdesi ötiroid grubuna kıyasla arttı (p<0.05). Altıncı hafta hipotiroid grubunda, alyuvar (RBC) sayısı, hemoglobin (Hb) konsantrasyonu, hematokrit (Hct) yüzdesi ve alyuvar dağılım genişliği (RDW) yüzdesi, 6. hafta ötiroid ve hipertiroid grubuna nazaran düşüktü (p<0.05). Ancak, 6. hafta hipotiroid grubundaki plateletkrit (PCT) yüzdesi, 6. hafta ötiroid grubuna kıyasla yüksekti (p<0.05). Altıncı hafta hipertiroid grubunda, ortalama alyuvar hemoglobin konsantrasyonu (MCHC) ve platelet (PLT) sayısı 6. hafta hipotiroid grubuna nazaran düşüktü (p<0.05). Altıncı hafta hipertiroid grubunda, 6. hafta ötiroid grubuna kıyasla lenfosit yüzdesi düşük (p<0.05) ve granülosit yüzdesi yüksekti (p<0.05). Sonuç olarak, propiltiourasil (PTU) ve L-tiroksin (L-T4) uygulama sürelerinin uzaması bazı hematolojik parametrelerde değişimlere sebep olmaktadır. Buna rağmen, bu değerler fizyolojik sınırlar içerisinde kalmaktadır
References
- Abou-auda HS, Abou-shaaban RRA (2006): Effect of abouthiouline a novel drug with therapeutic potential as antithyroid, on some biochemical and hematologic parameters in mice and rats. Saudi Pharm J, 14, 34-41.
- Bianco AC, Anderson G, Forrest D, et al. (2014): American Thyroid Association Guide to investigating thyroid hormone economy and action in rodent and cell models. Thyroid, 24, 88-168.
- Chandel RS, Gangadhar C, Leela A (2015): Impact of subclinical hematological parameters. APALM, 2, 21-25. on iron status and
- Das K, Chainy GBN (2004): Thyroid hormone influences antioxidant defense system in adult rat brain. Neurochem Res, 29, 1755-1766.
- Davis PJ, Leonard JL, Davis FB (2008): Mechanisms of nongenomic Neuroendocrinol, 29, 211-218. thyroid hormone. Front 6. Dixon RM, Reid SW, Mooney CT (1999): Epidemiological, clinical, haematological and biochemical characteristics of canine hypothyroidism. Vet Rec, 145, 481-487.
- Dönmez N, Keskin E (2009): The effects of aflatoxin and glucomannan on coagulation parameters in rabbits. Veterinarski Arhiv, 79, 555-560.
- El-Bakry AM, El-Gareib AW, Ahmed RG (2010): Comparative study of the effects of experimentally induced hypothyroidism and hyperthyroidism in some brain regions in albino rats. Int J Dev Neurosci, 28, 371-389.
- Fandrey J, Pagel H, Frede S, et al. (1994): Thyroid hormones production in vitro. Exp Hematol, 22, 272-277. erythropoietin
- Ferreira E, Silva AE, Serakides R, et al. (2007): Model of induction of thyroid dysfunctions in adult female mice. Arq Bras Med Vet Zootec, 59, 1245-1249.
- Flamant F, Baxter JD, Forrest D, et al. (2006): International pharmacology and classification of the nuclear receptor superfamily: Thyroid hormone receptors. Pharmacol Rev, 58, 705-711. Pharmacology. LIX. The
- Giknis MLA, Clifford CB (2008): Clinical Laboratory Parameters for Crl:Wl (Han). http://www.criver.com/files/pdfs/rms/wistarhan/rm_rm_r_ wistar_han_clin_lab_parameters_08.aspx.(20.03.2015)
- Golde D W, Bersch N, Chopra IJ, et al. (1977): Thyroid hormones stimulate erythropoiesis in vitro. Br J Haematol, 37, 173-177.
- Guerrero A, Pamplona R, Portero-Otin M, et al. (1999): Effect of thyroid status on lipid composition and peroxidation in the mouse liver. Free Radic Biol Med, 26, 73-80.
- Guyton AC, Hall JE (2011): Thyroid Metabolic Hormones. 931-943. In: Guyton and Hall Textbook of Medical Physiology, Elsevier, United States of America,.
- Hulbert AJ (2000): Thyroid hormones and their effects: A new perspective. Biological Reviews, 75, 519-631.
- Jawad KA, Atyaf JHA-Z (2015): The therapeutic role of alcoholic extract of fenugreek seeds on hypothyroidism state induced by thiourea and some blood parameters in adult male rabbits. The Iraqi J Vet Med, 39, 1-7.
- Kampfmann I, Bauer N, Johannes S, et al. (2012): Differences in hematologic variables in rats of the same strain but different origin. Vet Clin Pathol, 41, 228-234.
- Kawa MP, Grymula K, Paczkowska E, et al. (2010): Clinical relevance of thyroid dysfunction in human haematopoiesis: biochemical and molecular studies. Eur J Endocrinol, 162, 295-305.
- Kendrick TS, Payne CJ, Epis MR, et al. (2008): Erythroid defects in TRalpha-/- mice. Blood, 111, 3245- 3248.
- Klecha AJ, Genaro AM, Gorelik G, et al. (2006): Integrative immune system interaction: Thyroid hormone-mediated modulation of lymphocyte activity through the protein kinase C signaling pathway. J Endocrinol, 189, 45-55.
- Malgor LA, Blanc CC, Klainer E et al. (1975): Direct effects of thyroid hormones on bone marrow erythroid cells of rats. Blood, 45, 671-679.
- Manna D, Roy G, Mugesh G (2013): Antithyroid drugs and their analogues: Synthesis, structure, and mechanism of action. Accounts of Chemical Research, 46, 2706-2715.
- Marongiu F, Barcellona D, Mameli A et al. (2011): Thyroid disorders and hypocoagulability. Semin Thromb Hemost, 37, 11-16.
- Messarah M, Saoudi M, Boumendjel A, et al. (2011): Oxidative stress induced by thyroid dysfunction in rat erythrocytes and heart. Environ Toxicol Pharmacol, 31, 33-41.
- Moriyama K, Tagami T, Usui T, et al. (2007): Antithyroid drugs inhibit thyroid hormone receptor- mediated transcription. J Clin Endocrinol Metab, 92, 1066-1072.
- Mostaghni K, Bashari Maafi A, Badiei K (2005): Study of the effects of experimental hypothyroidism on clinical, hematological and serum biochemical factors in pregnant ewes. Iran J Vet Res, 6, 1-5, 73.
- Nambiar PR, Palanisamy GS, Okerberg C, et al. (2014): Toxicities associated with 1-month treatment with propylthiouracil (PTU) and methimazole (MMI) in male rats. Toxicol Pathol, 42, 970-983.
- Nejar Bruno A, Pochmann D, Klein Ricachenevsky F, et al. (2005): 5′-nucleotidase activity is altered by hypo- and hyperthyroidism in platelets from adult rats. Platelets, 16, 25-30.
- Noyan A (2011): Tiroid Bezi ve Hormonu. 1007-1033. In: Yaşamda ve Hekimlikte Fizyoloji, Palme Yayınları, Ankara.
- Oetting A, Yen PM (2007): New insights into thyroid hormone action. Best Pract Res Clin Endocrinol Metab, 21, 193-208.
- Osonuga IO, Olowookorun MO, Akinola BO (2014): Effects of thyroxine on blood parameters and liver enzymes in adult male and female rats. Afr J Biomed Res, 17, 53-56.
- Serakides R, Ocarino NM, Cardoso TGS, et al. (2005): Response of parathyroid to the plasmatic of calcium and hosphorus hypogonadism in the rat. Arq Bras Med Vet Zootec,
- 57, 48-54. in hyperthyroidism and Sullivan PS, McDonald TP (1992): Thyroxine suppresses thrombocytopoiesis and stimulates erythropoiesis in mice. Proc Soc Exp Biol Med, 201, 271-277.
- Umezu M, Kagabu S, Jiang J, et al. (1998): Evaluation and characterization of congenital hypothyroidism in rdw dwarf rats. Lab Anim Sci, 48, 496-501.
- Vennström B, Liu H, Forrest D (2010): Thyroid hormone receptors. 183-201 In: Chris M. Bunce MJC (Ed) Nuclear Receptors Current Consepts and Future Challenges. Springer, Netherlands.
- Würtz M, Hvas AM, Kristensen SD, et al. (2012): Platelet aggregation is dependent on platelet count in patients with coronary artery disease. Thromb Res, 129, 56-61.
- Yen PM (2001): Physiological and molecular basis of thyroid hormone action. Physiol Rev, 81, 1097-1142.
- Yücel R, Özdemir S, Darıyerli N, et al. (2009): Erythrocyte osmotic fragility and lipid peroxidation in experimental hyperthyroidism. Endocr, 36, 498-502.
- Zahediasl S, Habibi G, Ghasemi A, et al. (2010): Hematological parameters and osmotic fragility of red blood cells in experimentally induced hyperthyroidism in rats. Int J Endocrinol Metab, 8, 74-78.
There are 39 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA87KV54EK |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2016 |
| Published in Issue | Year 2016 |