Evaluation of antithrombotic and anti-ischemic effects of morinda citrifolia in renal autotransplantation in rats
Abstract
Although it is not performed as common as in humans, kidney transplantation is an effective treatment option for end-stage renal failure in dogs and cats. Ischemia/reperfusion injury is an inevitable result of kidney transplantation and it may cause delayed graft function that leads to a reduction in the longevity of allograft. In this study we aimed to evaluate the effects of Morinda citrifolia derived fruit juice on post-autotransplant ischemia/reperfusion injury. Twenty four adult, male Spraque-Dawley rats weighing 300-350 g were used. The rats were randomly divided into three groups of eight each. Rats in the Control Group were orally administered with saline solution (0.5 ml/day), for 7 days. Rats in M1 Group were orally administered with morinda citrifolia fruit juice (Alnoni, Hanojou-Europe Ltd, Dinxperlo, Holland), 0.5 ml/day, for 7 days whereas rats in M2 Group were orally administered with the same morinda citrifolia fruit juice (1 ml/day), for 7 days. In the 8th day, rats were anesthetized and orthotopic unilateral renal autotransplantation was performed. Rats were euthanized after 24 hour reperfusion and ischemic kidneys were sent for histopathological examination. Formalin-fixed paraffin-embedded tissue sections were evaluated and scored according to the parameters of tubular brush border loss, mononuclear cell infiltration, degeneration, hyalin formation in tubul lumens, thrombosis and tubular necrosis. Mononuclear cell infiltration in M2 group was found to be significantly different (P<0.05) than those of Control and M1 groups. Thrombosis was not observed in any of the histopathological sections. As a result, a significant improving effect on the ischemia/reperfusion injury of Morinda citrifolia fruit juice was not determined in rats after renal autotransplantation
References
- Bernsteen L, Gregory CR, Pollard RE, Griffey SM, Menwrath V (1999): Comparison of two surgical techniques for renal transplantation in cats. Vet Surg, 28, 417-420.
- Bhavani R, Nandhini S, Rojalakshmi B, Shobana R, Rajeshkumar S (2014): Effect of noni (Morinda citrifolia) extract on the treatment of ethylene glycol and ammonium chloride induced kidney disease. IJPSR, 5, 249-256.
- Dicken DH, Fuggle K, Fuggle S (2000): Impact of ischemia/reperfusion injury and early inflammatory responses in kidney transplantation. Transplant Rev, 14, 210-224.
- Gilani AH, Mandukhail SR, Iqbal J, et al. (2009): Antispasmodic and vasodilatator activities of Morinda citrifolia root extract are mediated through blockade of voltage dependent calcium chanels. BMC Complemantary and Altern Med, 10, 1-9.
- Giullian JA, Helderman JH (2008): Do calcium channel blockers prevent delayed graft function after renal transplantation? Nat Clin Pract Nephrol, 4, 192-193.
- Guan X, Dei-Anane G, Bruns H, et al. (2009): Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation. Transpl Int, 22, 232- 241.
- Harada S, Hamabe W, Kamiya K, et al. (2009): Preventive effect of morinda citrifolia fruit juice on neuronal damage induced by focal ischemia. Biol Pharm Bull, 32, 405-409.
- Harada S, Hamabe WF, Kamiya K, et al. (2010): Morinda citrifolia fruit juice prevents ischemic neuronal damage through suppression of the development of post- ischemic glucose intolerance. J Nat Med, 64, 468-473.
- Jang HR, Gandolfo MT, Ko GJ, et al. (2009): The effect of murine anti-thymocyte globulin on experimental kidney warm ischemia-reperfusion injury in mice. Transpl Immunol, 22, 44-54.
- Iwai S, Endo K, Hakamata Y, Gregory CR, Kobayashi E (2006): Use of nonpenetrating vascular closure staples in feline renal transplantation. Microsurgery, 26, 13-16.
- Li Z, Nickkholgh A, Yi X, et al. (2009): Melatonin protects kidney grafts from ischemia/reperfusion injury through inhibition of NF-kB and apoptosis after experimental kidney transplantation. J Pineal Res, 46, 365- 372.
- Luna L.G. (1968): Manual of histologic staining methods of the armed forces institute of pathology (3rd Ed). Blakiston Division, McGraw-Hill Book Company, Newyork.
- Nayak S, Mengi S (2010): Immunostimulant activity of noni (morinda citrifolia) on T and B lymphocytes. Pharm Biol, 2010, 48, 724-731.
- Nayak BS, Marshall JR, Isitor G, et al. (2011): Hypoglycemic and hepatoprotective activity of morinda citrifolia (noni) in diabetic rats. Evid Based Complement Alternat Med, 2011, 1-5.
- Neri F, Puviani L, Tsivian M, et al. (2007): Protective effect of inhibitor of interleukine-8 (Meraxin) from ischemia and reperfusion injury in a rat model of kidney transplantation. Transplant Proc, 39, 1771-1772.
- Sener G, Sehirli AO, Keyer-Uysal M, et al. (2002): The protective effect of melatonin on renal ischemia- reperfusion injury in the rat. J Pineal Res, 32, 120-126.
- Siedlecki A, Irish W, Brennan DC (2011): Delayed graft function in kidney transplant. Am J Transplant, 11, 2279- 2296.
- Taskin EI, Akgün-Dar K, Kapucu A, et al. (2009): Apoptosis-inducing effects of morinda citrifolia l. and doxorubucin on the Ehrlich ascites tumor in Balb-c mice. Cell Biochem Funct, 27, 542-546.
- Verma NK, Patel SS, Saleem TSM, Christina AJM, Chidambaranathan N (2009): Modulatory effect of noni- herbal formulation against ethylene glycol-induced nephrolithiasis in albino rats. J Pharm Sci, 1, 83-89
- Wang MY, West BJ, Jensen CJ, et al. (2002): Morinda citrifolia (Noni): A literature review and recent advances in Noni research. Acta Pharmacol Sin, 23, 1127-1141.
- West BJ, Su CX, Jarakae Jensen J (2009): Hepatotoxicity and subchronic toxicity tests of Morinda citrifolia (noni) fruit. J Toxicol Sci, 34, 581-585.
- Williams P, Lopez H, Britt D, et al. (1997): Characterization of renal ischemia-reperfusion injury in rats. JPM, 37, 1-7.
- Yarlagadda SG, Coca SG, Formica RN, et al. (2009): Association between delayed graft function and allograft and patient survival: A systematic review and meta- analysis. Nephrol Dial Transplant, 24, 1039-1047.
Renal ototransplantasyon yapılan sıçanlarda morinda citrifolia ‘nın antitrombotik ve anti-iskemiketkilerinin değerlendirilmesi
Abstract
Böbrek transplantasyonu, son dönem böbrek yetmezliğinin tedavisi amacıyla, insanlardaki kadar yaygın olmamakla birlikte, kedi ve köpekler için de kullanılan bir renal replasman tedavisidir. İskemi/reperfüzyon hasarı ise böbrek nakillerinde kaçınılmaz olarak karşımıza çıkan ve gecikmiş gref fonksiyon bozukluğuna neden olarak nakledilen organın yaşam süresini kısaltan bir etken olarak varlığını sürdürmektedir. Bu çalışma ile tropikal bir meyveden elde edilen Morinda citrifolia meyve suyunun böbrek ototransplantasyonu sonrası oluşan iskemi/reperfüzyon hasarı üzerine etkilerini değerlendirmek amaçlanmıştır. Bu maksatla ağırlıkları 300-350 g arasında değişen, 24 adet Spraque-Dawley ırkı erkek sıçan, Kontrol, M1 ve M2 olmak üzere 3 gruba ayrıldı. Preoperatif olarak, Kontrol grubundaki sıçanlara % 0,9 NaCl solüsyonu (0,5 ml/gün), M1 grubuna Morinda citrifolia meyve suyu (0,5 ml/gün), M2 grubuna ise yine Morinda citrifolia meyve suyu (1 ml/gün) oral yolla 7 gün boyunca uygulandı. Sıçanlar 8. gün anesteziye alınarak, ortotopik unilateral böbrek ototransplantasyonu gerçekleştirildi. Sıçanlara, 24 saatlik reperfüzyon süresi sonunda ötanazi işlemi uygulandı ve iskemik böbrekler alınarak histopatolojik incelemeye gönderildi. Kesitler, histopatolojik muayene sonuçlarına göre, tubül fırçamsı kenar kaybı, mononükleer hücre infiltrasyonu, dejenerasyon, tubul lumenleri içinde hyalin oluşumu, tromboz ve tubüler nekroz parametreleri açısından değerlendirilerek skorlandı. Mononükleer hücre infiltrasyonu açısından M2 grubu ile M1 ve Kontrol grubu arasındaki fark ise önemli bulundu (P<0,05). Histopatolojik kesitlerin hiçbirinde tromboz varlığına rastlanmadı. Sonuç olarak sıçanlarda, Morinda citrifolia meyve suyunun böbrek ototransplantasyonları sonrası oluşan, iskemi/reperfüzyon hasarı üzerine önemli bir iyileştirici etkisi olmadığı sonucuna varıldı
References
- Bernsteen L, Gregory CR, Pollard RE, Griffey SM, Menwrath V (1999): Comparison of two surgical techniques for renal transplantation in cats. Vet Surg, 28, 417-420.
- Bhavani R, Nandhini S, Rojalakshmi B, Shobana R, Rajeshkumar S (2014): Effect of noni (Morinda citrifolia) extract on the treatment of ethylene glycol and ammonium chloride induced kidney disease. IJPSR, 5, 249-256.
- Dicken DH, Fuggle K, Fuggle S (2000): Impact of ischemia/reperfusion injury and early inflammatory responses in kidney transplantation. Transplant Rev, 14, 210-224.
- Gilani AH, Mandukhail SR, Iqbal J, et al. (2009): Antispasmodic and vasodilatator activities of Morinda citrifolia root extract are mediated through blockade of voltage dependent calcium chanels. BMC Complemantary and Altern Med, 10, 1-9.
- Giullian JA, Helderman JH (2008): Do calcium channel blockers prevent delayed graft function after renal transplantation? Nat Clin Pract Nephrol, 4, 192-193.
- Guan X, Dei-Anane G, Bruns H, et al. (2009): Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation. Transpl Int, 22, 232- 241.
- Harada S, Hamabe W, Kamiya K, et al. (2009): Preventive effect of morinda citrifolia fruit juice on neuronal damage induced by focal ischemia. Biol Pharm Bull, 32, 405-409.
- Harada S, Hamabe WF, Kamiya K, et al. (2010): Morinda citrifolia fruit juice prevents ischemic neuronal damage through suppression of the development of post- ischemic glucose intolerance. J Nat Med, 64, 468-473.
- Jang HR, Gandolfo MT, Ko GJ, et al. (2009): The effect of murine anti-thymocyte globulin on experimental kidney warm ischemia-reperfusion injury in mice. Transpl Immunol, 22, 44-54.
- Iwai S, Endo K, Hakamata Y, Gregory CR, Kobayashi E (2006): Use of nonpenetrating vascular closure staples in feline renal transplantation. Microsurgery, 26, 13-16.
- Li Z, Nickkholgh A, Yi X, et al. (2009): Melatonin protects kidney grafts from ischemia/reperfusion injury through inhibition of NF-kB and apoptosis after experimental kidney transplantation. J Pineal Res, 46, 365- 372.
- Luna L.G. (1968): Manual of histologic staining methods of the armed forces institute of pathology (3rd Ed). Blakiston Division, McGraw-Hill Book Company, Newyork.
- Nayak S, Mengi S (2010): Immunostimulant activity of noni (morinda citrifolia) on T and B lymphocytes. Pharm Biol, 2010, 48, 724-731.
- Nayak BS, Marshall JR, Isitor G, et al. (2011): Hypoglycemic and hepatoprotective activity of morinda citrifolia (noni) in diabetic rats. Evid Based Complement Alternat Med, 2011, 1-5.
- Neri F, Puviani L, Tsivian M, et al. (2007): Protective effect of inhibitor of interleukine-8 (Meraxin) from ischemia and reperfusion injury in a rat model of kidney transplantation. Transplant Proc, 39, 1771-1772.
- Sener G, Sehirli AO, Keyer-Uysal M, et al. (2002): The protective effect of melatonin on renal ischemia- reperfusion injury in the rat. J Pineal Res, 32, 120-126.
- Siedlecki A, Irish W, Brennan DC (2011): Delayed graft function in kidney transplant. Am J Transplant, 11, 2279- 2296.
- Taskin EI, Akgün-Dar K, Kapucu A, et al. (2009): Apoptosis-inducing effects of morinda citrifolia l. and doxorubucin on the Ehrlich ascites tumor in Balb-c mice. Cell Biochem Funct, 27, 542-546.
- Verma NK, Patel SS, Saleem TSM, Christina AJM, Chidambaranathan N (2009): Modulatory effect of noni- herbal formulation against ethylene glycol-induced nephrolithiasis in albino rats. J Pharm Sci, 1, 83-89
- Wang MY, West BJ, Jensen CJ, et al. (2002): Morinda citrifolia (Noni): A literature review and recent advances in Noni research. Acta Pharmacol Sin, 23, 1127-1141.
- West BJ, Su CX, Jarakae Jensen J (2009): Hepatotoxicity and subchronic toxicity tests of Morinda citrifolia (noni) fruit. J Toxicol Sci, 34, 581-585.
- Williams P, Lopez H, Britt D, et al. (1997): Characterization of renal ischemia-reperfusion injury in rats. JPM, 37, 1-7.
- Yarlagadda SG, Coca SG, Formica RN, et al. (2009): Association between delayed graft function and allograft and patient survival: A systematic review and meta- analysis. Nephrol Dial Transplant, 24, 1039-1047.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA32HF98YV |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2016 |
| Published in Issue | Year 2016 |