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Determination of some acute phase proteins level in cattle with brucellosis

Year 2016, , 13 - 16, 01.03.2016

Kadir Bozukluhan Oğuz Merhan Fatih Büyük Özgür Çelebi Gürbüz Gökçe

https://doi.org/10.1501/Vetfak_0000002703

Abstract

The aim of the present study was to determine concentrations of some acute phase proteins (APP) and to investigate their diagnostic importance in cattle with brucellosis. For this purpose 20 cattle with brucellosis as working group and 10 healthy cattle as control group were used. Blood samples were collected from Jugular vein in tubes without anticoagulant. Blood samples were then centrifuged and the acquired serum samples were stored at -20 oC until future use. Samples were used for determine of concentration of haptoglobin (Hp), ceruloplasmin and albumin. Result of the present study indicated that the concentrations of positive APP including Hp, ceruloplasmin were significantly increased, and albumin concentration was significantly decreased in cattle with brusellosis when compared with healthy ones. In conclusion; in this study in animals infected with brucella occurs acute phase response and as a result Hp and ceruloplasmin synthesis increased while albumin synthesis is determined decreased

Keywords

Acute phase proteins brucellosis cattle

References

  • Alton GG, Jones ML, Angus RD, Verger JM (1988): Techniques for the brucellosis laboratory. INRA Publications, Paris, France.
  • Aydın N, İzgür M, Diker KS, Yardımcı H, Esendal ÖM, Parcıkoğlu J, Akan M (2006): Veteriner Mikrobiyo- loji. İlke Emek Yayınevi Yayınları, Ankara.
  • Baldwin, CL, Winter, AJ (1994): Macrophages and Brucella. Immunol Ser, 60, 363-380.
  • Bayraktar M, Bayraktar N, Bayındır Y, Durmaz R (2005): Brusellozlu hastalarda serum C reaktif protein, demir ve ferritin düzeylerinin tanı ve izlemdeki değeri. ANKEM Derg, 19, 61-63.
  • Bozukluhan K, Gökce HI (2007): Retikuloperitonitis travmatika ve retikuloperikarditis travmatika’lı sığırlarda bazı akut faz proteinlerin araştırılması. Erciyes Üniv Vet Fak Derg, 4, 107-113.
  • Bullen JJ (1981): The significance of iron in infection. Rev Infect Dis, 3, 1127-1138.
  • Campero CM, Ladds PW, Hoffmann D, Duffield B, Watson D, Fordyce G (1990): Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls. Vet Immunol Immunopathol, 24, 235- 246.
  • Colombo JP, Richterich R (1964): Zur bestimmung des caeruloplasmin im plasma (on the determination of ceruloplasmin in plasma). Schweiz Med Wochenschr, 94, 715-720.
  • Conner JG, Eckersall PD, Wiseman A, Bain RK, Douglas TA (1989): Acute phase response in calves following infection with Pasteurella haemolytica, Ostertagia ostertagia and endotoxin administration. Res Vet Sci, 47, 203-207.
  • Çoşkun A, Şen İ (2011): Sığırlarda akut faz proteinleri ve klinik kullanım alanları. Sağlık Bilimleri Dergisi, 20, 240- 246.
  • Eckersall PD, Bell R (2010): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27.
  • Eckersall PD, Conner JG (1988): Bovine and canine acute phase proteins. Vet Res Commun, 12, 169-178.
  • Fagliari JJ, Mcclenahan D, Evanson OA, Weiss DJ (1998): Changes in plasma protein concentrations in ponies with experimentally induced alimentary laminitis. Am J Vet Res, 59, 1234-1237.
  • Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP (2003): The acute phase response in calves experimentally infected with bovine viral diarrhoea virus and/or Manheimia haemolytica. J Vet Med Ser B, 50, 183– 190.
  • Gruys E, Obwolo MJ, Toussaint M (1994): Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry: a rewiev. Vet Bull, 64, 1009- 1018.
  • Gruys E, Toussaint MJM, Niewald TA (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci, 11, 1045-1056.
  • Isaac KQ (2008): Haptoglobin, inflammation and disease. Trans R Soc Trop Med Hyg, 102, 735-742.
  • Kuyucuoğlu Y (2011): Brusella hastalığı. Kocatepe Vete- riner Dergisi, 4, 57-64.
  • Murata H, Shimada N, Yoshioka M (2004): Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J, 168, 28-40.
  • Neta AVC, Mol JPS, Xavier MN, Paixao TA, Lage AP, Santos RL (2010): Pathogenesis of bovine brucellosis. Vet J, 184, 146-155.
  • Nicoletti P (2010): Brusellosis: past, present and future. Prilozi, 31, 21-32.
  • Nisbet C, Yarım GF, Çiftci A, Çenesiz S, Çiftci G (2007): Investigation of serum nitric oxide and malonaldialdehyde levels in cattle infected with Brucella abortus. Ankara Üniv Vet Fak Derg, 54, 159-163.
  • Petersen HH, Nielsen JP, Heegard PMH (2004): Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35, 163-187.
  • Pineiro M, Andres M, Iturralde M, Carmona S, Hirvonen J, Pyörala, S, Heegard PMH, Tjorneoj K, Pineiro A (2004): ITIH4 (Inter AlphaTrypsin Inhibitor Heavy Chain 4) is a new acute phase protein isolated from cattle during experimental infection. Infec Immun, 72, 3777-3782.
  • Poester FP, Samartino LE, Santos RL (2013): Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech Off Int Epiz, 32, 105-115.
  • Roussel AJ, Whitney MS, Cole D (1997): Interpreting a bovine serum chemistry profile: Part: 1. Vet Med, 92, 551- 558.
  • Sharifiyazdia H, Nazifi S, Nikseresht K, Shahriari R (2012): Evalution of serum amyloid A and haptoglobin in dairy cows naturally infected with brucellosis. J Bacteriol Parasitol, 3, 1-4.
  • Sheldon IM, Noakes DE, Bayliss M, Dobson H (2003): The effect of oestradiol on postpartum uterine involution in sheep. Anim Reprod Sci, 78, 57-70.
  • Sheldon IM, Noakes DE, Rycroft A, Dobson H (2001): Acute phase protein responses to uterine bacterial contamination in cattle after calving. Vet Rec, 148, 172- 175.
  • Skinner JG, Brown RA, Roberts L (1991): Bovine hap- toglobin response in clinically defined field conditions. Vet Rec, 128, 147-149.
  • SPSS (2008): SPSS for windows release 16.0.2. SPSS Inc., Chicago.
  • Tabak F, Hakko E, Mete B, Ozaras R, Mert A, Ozturk R (2008): Is family screening necessary in brucellosis? Infection, 36, 575-577.
  • Talaro, K, Talaro, A (1996): Foundations in Microbiology. Wm. C. Brown Publishers, USA.
  • Toussaint MJM, van Ederen AM, Gruys E (1995): Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Internat, 5, 149-157.
  • Trujillo IZ, Zavala AN, Caceres JG, Miranda CQ (1994): Brucellosis infection. Infect Dis Clin North Am, 8, 225-241.
  • Uluğ M, Uluğ NC, Selek Ş (2010): Akut brusellozlu has- talarda akut faz reaktanların düzeyi. Klinik Dergisi, 23, 48-50.
  • Ulutaş PA, Voyvoda H, Ulutaş B, Aypak S (2008): Miks enfeksiyonlu keçilerde haptoglobin, serum amiloid A, ve seruloplazmin konsantrasyonları. Türkiye Parazitol Derg, 32, 229-233.
  • Xavier MN, Paixao TA, Hartigh AB Den, Tsolis RM, Santos RL (2010): Pathogenesis of Brucella spp. Open Vet Sci J, 4, 109-118.
  • Yucayurt, R (2008): Brucellalı Sığırlarda Serum 5’Nükleotidaz (5’NT), Lösinaminopeptidaz (LAP), γ- Glutamiltransferaz (GGT), Alaninaminotransferaz (ALT) ve Aspartat aminotransferaz (AST) Aktivitelerinin Araştı- rılması, (Doktora Tezi). KAÜ Sağlık Bilimleri Enstitüsü, Kars.

Brusellozisli sığırlarda bazı akut faz proteinlerin düzeyinin saptanması

Year 2016, , 13 - 16, 01.03.2016

Kadir Bozukluhan Oğuz Merhan Fatih Büyük Özgür Çelebi Gürbüz Gökçe

https://doi.org/10.1501/Vetfak_0000002703

Abstract

Bu çalışmanın amacı brusella ile enfekte sığırlarda, bazı akut faz protein düzeylerinin belirlenmesi ve diagnostik önemlerinin araştırılmasıdır. Bu amaçla çalışmada 20 adet brusella ile enfekte ve 10 adet sağlıklı hayvan kullanıldı. Hayvanların V. jugularis’inden antikoagulansız tüplere kan örnekleri toplandı. Kan örnekleri -20 oC de saklandı. Hazırlanan serum örnekleri haptoglobin (Hp), albümin ve seruloplazmin ölçümlerinde kullanıldı. Yapılan analiz sonucunda brusella ile enfekte sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında Hp ve seruloplazmin değerlerinin kontrol grubuna göre konsantrasyonunun yükseldiği, albümin değerinin ise kontrol grubuna göre konsantrasyonunun düştüğü belirlendi. Sonuç olarak; yapılan bu çalışmada brusella ile enfekte hayvanlarda akut faz yanıtın oluştuğu ve bunun sonucu olarak Hp ve seruloplazmin sentezinin arttığı, albümin sentezinin ise düştüğü belirlenmiştir

Keywords

Akut faz proteinler brusellozis sığır

References

  • Alton GG, Jones ML, Angus RD, Verger JM (1988): Techniques for the brucellosis laboratory. INRA Publications, Paris, France.
  • Aydın N, İzgür M, Diker KS, Yardımcı H, Esendal ÖM, Parcıkoğlu J, Akan M (2006): Veteriner Mikrobiyo- loji. İlke Emek Yayınevi Yayınları, Ankara.
  • Baldwin, CL, Winter, AJ (1994): Macrophages and Brucella. Immunol Ser, 60, 363-380.
  • Bayraktar M, Bayraktar N, Bayındır Y, Durmaz R (2005): Brusellozlu hastalarda serum C reaktif protein, demir ve ferritin düzeylerinin tanı ve izlemdeki değeri. ANKEM Derg, 19, 61-63.
  • Bozukluhan K, Gökce HI (2007): Retikuloperitonitis travmatika ve retikuloperikarditis travmatika’lı sığırlarda bazı akut faz proteinlerin araştırılması. Erciyes Üniv Vet Fak Derg, 4, 107-113.
  • Bullen JJ (1981): The significance of iron in infection. Rev Infect Dis, 3, 1127-1138.
  • Campero CM, Ladds PW, Hoffmann D, Duffield B, Watson D, Fordyce G (1990): Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls. Vet Immunol Immunopathol, 24, 235- 246.
  • Colombo JP, Richterich R (1964): Zur bestimmung des caeruloplasmin im plasma (on the determination of ceruloplasmin in plasma). Schweiz Med Wochenschr, 94, 715-720.
  • Conner JG, Eckersall PD, Wiseman A, Bain RK, Douglas TA (1989): Acute phase response in calves following infection with Pasteurella haemolytica, Ostertagia ostertagia and endotoxin administration. Res Vet Sci, 47, 203-207.
  • Çoşkun A, Şen İ (2011): Sığırlarda akut faz proteinleri ve klinik kullanım alanları. Sağlık Bilimleri Dergisi, 20, 240- 246.
  • Eckersall PD, Bell R (2010): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27.
  • Eckersall PD, Conner JG (1988): Bovine and canine acute phase proteins. Vet Res Commun, 12, 169-178.
  • Fagliari JJ, Mcclenahan D, Evanson OA, Weiss DJ (1998): Changes in plasma protein concentrations in ponies with experimentally induced alimentary laminitis. Am J Vet Res, 59, 1234-1237.
  • Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP (2003): The acute phase response in calves experimentally infected with bovine viral diarrhoea virus and/or Manheimia haemolytica. J Vet Med Ser B, 50, 183– 190.
  • Gruys E, Obwolo MJ, Toussaint M (1994): Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry: a rewiev. Vet Bull, 64, 1009- 1018.
  • Gruys E, Toussaint MJM, Niewald TA (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci, 11, 1045-1056.
  • Isaac KQ (2008): Haptoglobin, inflammation and disease. Trans R Soc Trop Med Hyg, 102, 735-742.
  • Kuyucuoğlu Y (2011): Brusella hastalığı. Kocatepe Vete- riner Dergisi, 4, 57-64.
  • Murata H, Shimada N, Yoshioka M (2004): Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J, 168, 28-40.
  • Neta AVC, Mol JPS, Xavier MN, Paixao TA, Lage AP, Santos RL (2010): Pathogenesis of bovine brucellosis. Vet J, 184, 146-155.
  • Nicoletti P (2010): Brusellosis: past, present and future. Prilozi, 31, 21-32.
  • Nisbet C, Yarım GF, Çiftci A, Çenesiz S, Çiftci G (2007): Investigation of serum nitric oxide and malonaldialdehyde levels in cattle infected with Brucella abortus. Ankara Üniv Vet Fak Derg, 54, 159-163.
  • Petersen HH, Nielsen JP, Heegard PMH (2004): Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35, 163-187.
  • Pineiro M, Andres M, Iturralde M, Carmona S, Hirvonen J, Pyörala, S, Heegard PMH, Tjorneoj K, Pineiro A (2004): ITIH4 (Inter AlphaTrypsin Inhibitor Heavy Chain 4) is a new acute phase protein isolated from cattle during experimental infection. Infec Immun, 72, 3777-3782.
  • Poester FP, Samartino LE, Santos RL (2013): Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech Off Int Epiz, 32, 105-115.
  • Roussel AJ, Whitney MS, Cole D (1997): Interpreting a bovine serum chemistry profile: Part: 1. Vet Med, 92, 551- 558.
  • Sharifiyazdia H, Nazifi S, Nikseresht K, Shahriari R (2012): Evalution of serum amyloid A and haptoglobin in dairy cows naturally infected with brucellosis. J Bacteriol Parasitol, 3, 1-4.
  • Sheldon IM, Noakes DE, Bayliss M, Dobson H (2003): The effect of oestradiol on postpartum uterine involution in sheep. Anim Reprod Sci, 78, 57-70.
  • Sheldon IM, Noakes DE, Rycroft A, Dobson H (2001): Acute phase protein responses to uterine bacterial contamination in cattle after calving. Vet Rec, 148, 172- 175.
  • Skinner JG, Brown RA, Roberts L (1991): Bovine hap- toglobin response in clinically defined field conditions. Vet Rec, 128, 147-149.
  • SPSS (2008): SPSS for windows release 16.0.2. SPSS Inc., Chicago.
  • Tabak F, Hakko E, Mete B, Ozaras R, Mert A, Ozturk R (2008): Is family screening necessary in brucellosis? Infection, 36, 575-577.
  • Talaro, K, Talaro, A (1996): Foundations in Microbiology. Wm. C. Brown Publishers, USA.
  • Toussaint MJM, van Ederen AM, Gruys E (1995): Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Internat, 5, 149-157.
  • Trujillo IZ, Zavala AN, Caceres JG, Miranda CQ (1994): Brucellosis infection. Infect Dis Clin North Am, 8, 225-241.
  • Uluğ M, Uluğ NC, Selek Ş (2010): Akut brusellozlu has- talarda akut faz reaktanların düzeyi. Klinik Dergisi, 23, 48-50.
  • Ulutaş PA, Voyvoda H, Ulutaş B, Aypak S (2008): Miks enfeksiyonlu keçilerde haptoglobin, serum amiloid A, ve seruloplazmin konsantrasyonları. Türkiye Parazitol Derg, 32, 229-233.
  • Xavier MN, Paixao TA, Hartigh AB Den, Tsolis RM, Santos RL (2010): Pathogenesis of Brucella spp. Open Vet Sci J, 4, 109-118.
  • Yucayurt, R (2008): Brucellalı Sığırlarda Serum 5’Nükleotidaz (5’NT), Lösinaminopeptidaz (LAP), γ- Glutamiltransferaz (GGT), Alaninaminotransferaz (ALT) ve Aspartat aminotransferaz (AST) Aktivitelerinin Araştı- rılması, (Doktora Tezi). KAÜ Sağlık Bilimleri Enstitüsü, Kars.
There are 39 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA58YZ94YA
Journal Section Research Article
Authors

Kadir Bozukluhan

Oğuz Merhan

Fatih Büyük

Özgür Çelebi

Gürbüz Gökçe

Publication Date March 1, 2016
Published in Issue Year 2016

Cite

APA Bozukluhan, K., Merhan, O., Büyük, F., Çelebi, Ö., et al. (2016). Determination of some acute phase proteins level in cattle with brucellosis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(1), 13-16. https://doi.org/10.1501/Vetfak_0000002703
AMA Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G. Determination of some acute phase proteins level in cattle with brucellosis. Ankara Univ Vet Fak Derg. March 2016;63(1):13-16. doi:10.1501/Vetfak_0000002703
Chicago Bozukluhan, Kadir, Oğuz Merhan, Fatih Büyük, Özgür Çelebi, and Gürbüz Gökçe. “Determination of Some Acute Phase Proteins Level in Cattle With Brucellosis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 1 (March 2016): 13-16. https://doi.org/10.1501/Vetfak_0000002703.
EndNote Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G (March 1, 2016) Determination of some acute phase proteins level in cattle with brucellosis. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 1 13–16.
IEEE K. Bozukluhan, O. Merhan, F. Büyük, Ö. Çelebi, and G. Gökçe, “Determination of some acute phase proteins level in cattle with brucellosis”, Ankara Univ Vet Fak Derg, vol. 63, no. 1, pp. 13–16, 2016, doi: 10.1501/Vetfak_0000002703.
ISNAD Bozukluhan, Kadir et al. “Determination of Some Acute Phase Proteins Level in Cattle With Brucellosis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/1 (March 2016), 13-16. https://doi.org/10.1501/Vetfak_0000002703.
JAMA Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G. Determination of some acute phase proteins level in cattle with brucellosis. Ankara Univ Vet Fak Derg. 2016;63:13–16.
MLA Bozukluhan, Kadir et al. “Determination of Some Acute Phase Proteins Level in Cattle With Brucellosis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 1, 2016, pp. 13-16, doi:10.1501/Vetfak_0000002703.
Vancouver Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G. Determination of some acute phase proteins level in cattle with brucellosis. Ankara Univ Vet Fak Derg. 2016;63(1):13-6.