Türkiye’deki rahvan yürüyüşlü atlarda doublesex and mab-3 related transcription factor 3 (DMRT3) mutant allel dağılımı
Abstract
Türkiye’deki rahvan yürüyüşlü ve değişik orijinlere sahip 265 atta DMRT3 mutant allel dağılımını incelemek amacıyla yapılan bu çalışmada, 182 Türkiye, 31 İran, 24 Afganistan ve 28 Bulgaristan orijinli at kullanılmıştır. Kan ve kıldan DNA izolasyonu yapılarak Single Nucleotide Polymorphism (SNP) genotiplendirmesi gerçekleştirilmiştir. SNP genotiplendirmesi 5’nükleaz yöntemine göre yapılmış, her örnek için bir yabanıl tip, bir de mutant tip Polymerase Chain Reaction (PCR) karışımı hazırlanmış ve genotiplendirmeler bu karışımlara göre okunmuştur. Homozigot mutant genotip (AA) oranları orijinlere göre sırasıyla, %85.71, 96.77, 91.67 ve 96.43; heterozigot mutant genotip (CA) oranları %9.89, 3.23, 8.33 ve 0.00 olurken; homozigot yabanıl genotip (CC) oranları %4.40, 0.00, 0.00 ve 3.57 olarak tespit edilmiştir. Mutant allel (A) frekansları ise aynı sıraya göre %90.70, 98.40, 95.80 ve 96.40 olarak hesaplanmıştır. Hardy-Weinberg eşitliğine uyum için yapılan ki-kare analizinde Türkiye ve Bulgaristan orijinli atların genetik dengede olmadığı saptanmıştır (P<0.001). İncelenen farklı orijinli atlarda mutant allel olan “A” allelinin frekansı çok yüksek düzeyde (0.907-0.984) bulunduğundan bu atların tamamı “alternatif yürüyüşlü atlar” olarak tanımlanmıştır. Gerek homozigot (CC) ve gerekse heterozigot (CA) yabanıl tip allele sahip bireylerden bazılarının da rahvan yürüyüşe sahip olması bu genotiplerde rahvan yürüyüş ile genotip ilişkisinin araştırılması gerekliliğine işaret etmektedir. Bu çalışmayla Türkiye’deki atlarda DMRT3 mutant allel varlığı belirlenmiş ve böylelikle DMRT3 genotiplerinin önceden belirlenebileceği, ayrıca kolay ve kısa zamanda pekiştirilmiş rahvan yürüyüş yapabilen atlar elde etmek amacıyla kullanılabileceği ortaya konmuştur
References
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- Petersen JL, Mickelson JR, Rendahl AK, Valberg SJ, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, Machado AC, Capomaccio S, Cappelli K, Cothran EG, Distl O, Fox-Clipsham L, Graves KT, Guérin G, Haase B, Hasegawa T, Hemmann K, Hill EW, Leeb T, Lindgren G, Lohi H, Lopes MS, McGivney BA, Mikko S, Orr N, Penedo MCT, Piercy RJ, Raekallio M, Rieder S, Rİed KH, Swinburne J, Tozaki T, Vaudin M, Wade CM, McCue ME (2013): Genome-wide analysis reveals selection for important traits in domestic horse breeds. PLOS Genetics. DOI: 10.1371/journal.pgen.1003211.
- Promerová M, Andersson LS, Juras R, Penedo MCT, Reissmann M, Tozaki T, Bellone R, Dunner S, Hořín P, Imsland F, Imsland P, Mikko S, Modrý D, Roed KH, Schwochow D,. Vega-Pla JL, Mehrabani-Yeganeh H, Yousefi-Mashouf N, Cothran EG, Lindgren G, Andersson L (2014): Worldwide frequency distribution of the ‘Gait keeper’ mutation in the DMRT3 gene. Animal Genetics, 45, 274–282. Geliş tarihi: 15.09.2014 / Kabul tarihi: 23.03.2015
Mutant doublesex and mab-3 related transcription factor (DMRT3) allele distribution of pacing horses in Turkey
Abstract
The study was carried out to investigate the DMRT3 mutant allele distribution of 265 pacing horses in Turkey from different origins (182 Turkish, 31 Iranian, 24 Afghan and 28 Bulgarian) were used in the study. Single Nucleotide Polymorphism (SNP) genotyping was determined according to the method of 5ʹ nuclease by DNA isolation from blood/hair samples. A wild type and also one mutant type Polymerase Chain Reaction (PCR) mix was prepared for each sample and the genotypes were read by using these mixtures. According to origins, the genotype percentages were determined as 85.71, 96.77, 91.67 and 96.43 % for homozygote mutant (AA); 9.89, 3.23, 8.33 and 0.00 % for heterozygote mutant (CA); 4.40, 0.00, 0.00 and 3.57 % for homozygote wild (CC), respectively. Mutant allele (A) frequencies were calculated as 90.70, 98.40, 95.80 and 96.40%, in the same order. Horses that Turkish and Bulgarian origins were not in the genetic equilibrium (P˂0.001). Mutant allele (A) frequencies were very high level (0.907-0.984) in examined different origin horses, so all these horses were defined as “alternative walking horses”. Some of the horses had homozygote (CC) or heterozygote (CA) wild type allele had showed pacing gait indicated that relationships of pacing and genotype should be investigated in these genotypes. In conclusion, the presence of DMRT3 mutant allele in pacing horses in Turkey was detected for the first time. Thus, DMRT3 genotypes might be predetermined and those genotypes could be used to get pacing or gated horses easily and as soon as possible
References
- Albertsdóttir E, Eriksson S, Sigurdsson Á, Árnason T (2011): Genetic analysis of ‘breeding field test status’ in Icelandic horses. Journal of Animal Breeding and Genetics, 128, 124-132.
- Andersson LS (2012): Equine Trait Mapping. From Dise- ase Loci to the Discovery of a Major Gene Controlling Vertebrate University of Agricultural Sciences, Uppsala. Doctoral Thesis, Swedish
- Andersson LS, Larhammar M, Memic F, Wootz H, Schwochow D, Rubin C-J, Patra K, Arnason T, Wellbring L, Hjälm G, Imsland F, Petersen JL, McCue ME, Mickelson JR, Cothran G, Ahituv N, Roepstorff L, Mikko S, Vallstedt A, Lindgren G, Andersson L, Kullander K (2012): Mutations in DMRT3 affect locomotion in horses and spinal circuit function in mice. Nature, 488, 642–646.
- Anonim (2014b): QIAamp DNA Mini Kit. Erişim Adresi: www.qiagen.com/products/catalog/sample- technologies/dna-sample-technologies/genomic- dna/qiaamp-dna-mini-kit Erişim Tarihi: 07.08.2014
- Antikas TG (2013): Native Greek horses: from man- and fish-eaters B.C. to DMRT3 gaiters in modern times. International Conference (Szürkék, rackák, mangalicák - Nemzetközi konferencia Matolcsi János tiszteletére), Budapest.
- Bateson W (1907): Trotting and pacing: Dominant and recessive? Science, 26, 908.
- Batu S (1962): Türk Atları ve At Yetiştirme Bilgisi. ss: 93, 95. Ankara.
- Çağlayan T, İnal S, Garip M, Coşkun B, İnal F, Günlü A, Güleç E (2010): The determination of situation and breed characteristics of Turkish Rahvan horse in Turkey. J Anim Vet Adv, 9, 674-680.
- Güleç E (1996): Türk Rahvan Atı ve Atçılığı. ISBN: 975- 95931-5-7, Ankara.
- Hendricks BL (1995): International Encyclopedia of Horse Breeds. University of Oklahoma Press, Norman.
- Higgins JA, Hubalek Z, Halouzka J, Elkins KL, Sjostedt A, Shipley M, Ibrahim MS (2000): Detection of Francisella tularensis in infected mammals and vectors using a probe-based polymerase chain reaction. The American Journal of Tropical Medicine and Hygiene, 62, 310–318.
- Nei M (1987): Molecular Evolutionary Genetics. Columbia University Press, New York.
- Özbeyaz C, Yıldız MA, Çamdeviren H (2001): Türki- ye’de yetiştirilen farklı Esmer sığır sürüleri arasındaki ge- netik ilişkiler. Turkish Journal of Veterinary and Animal Sciences, 25, 453-461.
- Özbeyaz C (2005): At Yetiştiriciliği Ders Notları. Ankara Üniversitesi Veteriner Fakültesi, Zootekni Anabilim Dalı, Ankara.
- Özbeyaz C, Akçapınar H, Yıldız MA, Yüceer B, Yaralı C (2013): Türkiye’de Rahvan Koşan Atlar Arasındaki Fe- notipik ve Genetik Çeşitlilik. TÜBİTAK-TOVAG, Proje No: 110O824, Sonuç Raporu, Ankara.
- Petersen JL, Mickelson JR, Rendahl AK, Valberg SJ, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, Machado AC, Capomaccio S, Cappelli K, Cothran EG, Distl O, Fox-Clipsham L, Graves KT, Guérin G, Haase B, Hasegawa T, Hemmann K, Hill EW, Leeb T, Lindgren G, Lohi H, Lopes MS, McGivney BA, Mikko S, Orr N, Penedo MCT, Piercy RJ, Raekallio M, Rieder S, Rİed KH, Swinburne J, Tozaki T, Vaudin M, Wade CM, McCue ME (2013): Genome-wide analysis reveals selection for important traits in domestic horse breeds. PLOS Genetics. DOI: 10.1371/journal.pgen.1003211.
- Promerová M, Andersson LS, Juras R, Penedo MCT, Reissmann M, Tozaki T, Bellone R, Dunner S, Hořín P, Imsland F, Imsland P, Mikko S, Modrý D, Roed KH, Schwochow D,. Vega-Pla JL, Mehrabani-Yeganeh H, Yousefi-Mashouf N, Cothran EG, Lindgren G, Andersson L (2014): Worldwide frequency distribution of the ‘Gait keeper’ mutation in the DMRT3 gene. Animal Genetics, 45, 274–282. Geliş tarihi: 15.09.2014 / Kabul tarihi: 23.03.2015
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA65ZC45VY |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2016 |
| Published in Issue | Year 2016 |