The effect of long term antibiotic treatment on bacteriological cure and somatic cell count atsubclinical mastitis due to Staphylococcus aureus in lactating dairy cows
Abstract
In this study, the effect of extended antibiotic treatment on bacteriological cure and SCC in lactating dairy cows suffering from subclinical mastitis due to S. aureus was investigated. As material, a total of 100 S. aureus isolated subclinically infected udder lobes were used. Before and after the treatment, milk samples were taken from subclinically infected udder lobes for bacteriological examination and somatic cell counting. Amoxicillin+clavulanic acid combination, determined as sensitive by the antibiogram, was administered both intramammarily and intramuscularly once a day during 7 days to udder lobes infected with S. aureus subclinically. Before treatment and also on 21th day after treatment milk samples were taken aseptically in order to determine bacteriological cure rate and somatic cell counts. At the end of the study, mean SCCs before and after treatment were determined as 300.500 cell/ml (min-max 1.000-992.000 cell/ml) and 183.000 cell/ml (min-max 2.000-6.574.000 cell/ml), respectively. Additionally, the bacteriological cure rate was %66. The difference with regard to mean SCC values between the two samplings was statistically significant (p<0.05). After the treatment, average SCC in S. aureus isolated and non-isolated milk samples were 251.500 cells/ml and 159.500 cell/ml and these differences were not statistically significant. In conclusion, it was determined that long term antibiotic treatment causes an increase at bacteriological cure rate but a decrease at SCC at subclinical mastitis cases due to S. aureus
References
- Barkema HW, Schukken YH, Zadoks RN (2006): Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci, 89, 1877-1895.
- Baştan A (2002): İneklerde Mastitis. Hatiboğlu Yayınevi, Ankara.
- Baştan A (2013): İneklerde Meme Sağlığı ve Sorunları. 2. Baskı. Kardelen Ofset, Ankara.
- Catracchia C, Pol M, Tirante L, Chaves J (2009): Efficacy of tylosin treatment and factors affecting cure rate of Staphylococcus aureus subclinical mastitis. National Mastitis 48th Annual Meeting Proceedings, Charlotte, North Carolina.
- Done SH, Jacobs DE, Ikede BO, Mckenzie RA, Colwell D, Osweiler G, Bildfell RJ (2007): Diseases of The Mammary Gland. 673-762. In: OM Radostits, CC Gay, KW Hinchcliff, PD Constable (Ed), Veterinary Medicine. 10th Ed, Saunders Elsevier, Philadelphia.
- Foster RA (2006): Female Reproductive System. In: MD McGavin, JF Zachary (Ed), Pathologic Basis of Veterinary Disease. 4th Ed, Academic Press, New-York.
- Gillespie BE, Moorehead H, Lunn P, Dowlen HH, Johnson DL, Lamar KC, Lewis MJ, Ivey SJ, Hallberg JW, Chester ST, Oliver SP (2002): Efficacy of extended pirlimycin hydrochloride therapy for treatment of envi- ronmental Streptococcus spp and Staphylococcus aureus intramammary infections in lactating dairy cows. Veterinary Therapeutics, 3, 373-380.
- Greene WA, Gano AM, Smith KL, Hogan JS, Todhunter DA (1991): Comparison of probiotic and antibiotic intramammary therapy of cattle with elevated somatic cell counts. J Dairy Sci, 74, 2976-2981.
- Gruet P, Maincent P, Berthelot X, Kaltsatos V (2001): Bovine mastitis and intramammary drug delivery: review and perspectives. Adv Drug Deliv Rev, 50, 245-259.
- Harmon RJ, Eberhart RJ, Langlois DE, Wilson RA (1990): Microbiological procedures for the diagnosis of udder infection. National Mastitis Council, Arlington, VA.
- Haveri M (2008): Staphylococcus aureus in bovine intramammary epidemiological characteristics. Academic Dissertation, Helsinki. molecular, clinical and
- Haveri M, Suominen S, Rantala L, Honkanen-Buzalski T, Pyorala S (2005): Comparison of phenotypic and genotypic detection of penicilin G resistance of Staphylococcus intramammary infection. Vet Microbiol, 106, 97-102.
- Mevius D, Sampimon O, Sol J (2007): Antimicrobial resistance in mastitis organisms as a public health threat. 102-108. In: H Hogeveen (Ed), Mastitis in Dairy Production. 2nd Ed, Wageningen Academic Publishers, The Netherlands.
- Moret-Stalder S, Fournier C, Miseraz R, Albini S, Doherr MG, Reist M, Schaeren W, Kirchhofer M, Graber HU, Steiner A, Kaufmann T (2009): Prevalence study of Staphylococcus aureus in quarter milk samples of dairy cows in the Canton of Bern, Switzerland. Prev Vet Med, 88, 72-76.
- Owens WE, Ray CH, Watts JL, Yancey RJ (1997): Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. J Dairy Sci, 80, 313-317.
- Owens WE, Watts JL, Boddie RL, Nickerson SC (1988): Antibiotic treatment of mastitis: Comparison of intramammary and intramammary plus intramuscular therapies. J Dairy Sci, 71, 3143-3147.
- Pinho L, Ferreira J, Cabral C, Lameira R, Meireles P, Vaz F, Carvalheira J, Thompson G (2008): Prevalence of mastitis pathogens in milk samples from Portuguese dairy cattle. 47-53. In: TJGM Lam (Ed), Mastitis Control From Science to Practice. 1st Ed, Wageningen Academic Publishers, The Netherlands.
- Roy JP, Coteaux L, Tremblay D, Beaudry F, Elsener J (2009): Efficacy of a 5-day extended therapy program during lactation with cephapirin sodium in dairy cows chronically infected with Staphylococcus aureus. Can Vet J, 50, 1257-1262.
- Sandgren CH, Waller KP, Emanuelson U (2008): The- rapeutic antimicrobial treatment of bovine subclinical mastitis ments with no treatment for bacteriological efficacy during lactation. Vet J, 175, 108-117. against bovine mastitis pathogens. J Dairy Sci, 82, 1664
- Sekkin S, Kum C, Kırkan Ş, Güleş Ö, Akar F (2010): Sütçü ineklerde S. aureus’un neden olduğu subklinik masti- tisin sağaltımında sefoperazon-danofloksasin kombinasyo- nunun etkinliği. Kafkas Univ Vet Fak Derg, 16, 97-104.
- Sol J, Sampimon OC, Snoep JJ, Schukken YH (1997): Factors associated with bacteriological cure during Germany. lactation after therapy for subclinical mastitis caused by Staphylococcus aureus. J Dairy Sci, 80, 2803-2808. Geliş tarihi: 02.05.2014/ Kabul tarihi: 06.11.2014
- Swinkels JM, Cox P, Schukken YH, Lam TJ (2013): Efficacy of extended cefquinome treatment of clinical Staphylococcus aureus mastitis. J Dairy Sci, 96, 4983- 4992.
- Wilson DJ, Gonzalez RN, Case KL, Garrison LL, Grohn YT (1999): Comparison of seven antibiotic treat- 1670.
- Zecconi A, Piccinini R (2002): Intramammary Infections: Epidemiology and Diagnosis. 346-359. In: M Kaske (Ed), Recent Developments and Perspectives in Bovine Medicine. XIIth World Buiatrics Congress, Hannover,
Laktasyon dönemindeki sütçü ineklerde Staphylococcus aureus’a bağlı subklinik mastitislerde uzun süreli antibiyotik tedavisinin bakteriyolojik iyileşme ve somatik hücre sayısı üzerine etkisi
Abstract
Bu çalışmada, laktasyon dönemindeki sütçü ineklerde S. aureus’un neden olduğu subklinik mastitislerin sağaltımında, uzun süreli antibiyotik kullanımının bakteriyolojik iyileşme ve somatik hücre sayısı (SHS) üzerine etkisi araştırıldı. Çalışmada materyal olarak, subklinik mastitisli ve S. aureus izole edilen toplam 100 adet meme lobu kullanıldı. Subklinik mastitis saptanan meme loblarından tedavi öncesi ve sonrası bakteriyolojik muayene ve somatik hücre sayımı için süt örnekleri alındı. Çalışmada subklinik enfekte ve S. aureus izole edilen meme loblarına antibiyogram sonucuna göre duyarlı olduğu belirlenen amoksisilin+klavulanik asit kombinasyonu meme içi ve intramuskuler (IM) yolla günde bir kez, 7 gün süreyle uygulandı. Tedaviden önce ve tedavi bitiminden 21 gün sonra, aseptik koşullarda ilgili meme loblarından süt örneği alınarak, bakteriyolojik iyileşme oranı ve somatik hücre sayıları belirlendi. Çalışma sonucunda tedavi öncesi ortalama SHS ortanca değeri 300.500 hücre/ml (min-max 1.000-992.000 hücre/ml), tedavi sonrası 183.000 hücre/ml (min-max 2.000-6.574.000 hücre/ml) ve bakteriyolojik iyileşme oranı %66 olarak tespit edildi. Tedavi öncesi ve sonrası ortalama SHS değerleri bakımından farkın istatistiki olarak anlamlı olduğu belirlendi (p<0.05). Tedavi sonrası S. aureus izole edilen ve edilmeyen sütlerin ortalama SHS 251.500 hücre/ml ve 159.500 hücre/ml idi ve bu farklılık istatistiki açıdan anlamlı değildi (p>0.05). Sonuç olarak, uzun süreli kombine antibiyotik tedavisinin S. aureus’a bağlı subklinik mastitislerde bakteriyolojik iyileşme oranında artışa, SHS’nda ise azalmaya yol açtığı tespit edildi
References
- Barkema HW, Schukken YH, Zadoks RN (2006): Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci, 89, 1877-1895.
- Baştan A (2002): İneklerde Mastitis. Hatiboğlu Yayınevi, Ankara.
- Baştan A (2013): İneklerde Meme Sağlığı ve Sorunları. 2. Baskı. Kardelen Ofset, Ankara.
- Catracchia C, Pol M, Tirante L, Chaves J (2009): Efficacy of tylosin treatment and factors affecting cure rate of Staphylococcus aureus subclinical mastitis. National Mastitis 48th Annual Meeting Proceedings, Charlotte, North Carolina.
- Done SH, Jacobs DE, Ikede BO, Mckenzie RA, Colwell D, Osweiler G, Bildfell RJ (2007): Diseases of The Mammary Gland. 673-762. In: OM Radostits, CC Gay, KW Hinchcliff, PD Constable (Ed), Veterinary Medicine. 10th Ed, Saunders Elsevier, Philadelphia.
- Foster RA (2006): Female Reproductive System. In: MD McGavin, JF Zachary (Ed), Pathologic Basis of Veterinary Disease. 4th Ed, Academic Press, New-York.
- Gillespie BE, Moorehead H, Lunn P, Dowlen HH, Johnson DL, Lamar KC, Lewis MJ, Ivey SJ, Hallberg JW, Chester ST, Oliver SP (2002): Efficacy of extended pirlimycin hydrochloride therapy for treatment of envi- ronmental Streptococcus spp and Staphylococcus aureus intramammary infections in lactating dairy cows. Veterinary Therapeutics, 3, 373-380.
- Greene WA, Gano AM, Smith KL, Hogan JS, Todhunter DA (1991): Comparison of probiotic and antibiotic intramammary therapy of cattle with elevated somatic cell counts. J Dairy Sci, 74, 2976-2981.
- Gruet P, Maincent P, Berthelot X, Kaltsatos V (2001): Bovine mastitis and intramammary drug delivery: review and perspectives. Adv Drug Deliv Rev, 50, 245-259.
- Harmon RJ, Eberhart RJ, Langlois DE, Wilson RA (1990): Microbiological procedures for the diagnosis of udder infection. National Mastitis Council, Arlington, VA.
- Haveri M (2008): Staphylococcus aureus in bovine intramammary epidemiological characteristics. Academic Dissertation, Helsinki. molecular, clinical and
- Haveri M, Suominen S, Rantala L, Honkanen-Buzalski T, Pyorala S (2005): Comparison of phenotypic and genotypic detection of penicilin G resistance of Staphylococcus intramammary infection. Vet Microbiol, 106, 97-102.
- Mevius D, Sampimon O, Sol J (2007): Antimicrobial resistance in mastitis organisms as a public health threat. 102-108. In: H Hogeveen (Ed), Mastitis in Dairy Production. 2nd Ed, Wageningen Academic Publishers, The Netherlands.
- Moret-Stalder S, Fournier C, Miseraz R, Albini S, Doherr MG, Reist M, Schaeren W, Kirchhofer M, Graber HU, Steiner A, Kaufmann T (2009): Prevalence study of Staphylococcus aureus in quarter milk samples of dairy cows in the Canton of Bern, Switzerland. Prev Vet Med, 88, 72-76.
- Owens WE, Ray CH, Watts JL, Yancey RJ (1997): Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. J Dairy Sci, 80, 313-317.
- Owens WE, Watts JL, Boddie RL, Nickerson SC (1988): Antibiotic treatment of mastitis: Comparison of intramammary and intramammary plus intramuscular therapies. J Dairy Sci, 71, 3143-3147.
- Pinho L, Ferreira J, Cabral C, Lameira R, Meireles P, Vaz F, Carvalheira J, Thompson G (2008): Prevalence of mastitis pathogens in milk samples from Portuguese dairy cattle. 47-53. In: TJGM Lam (Ed), Mastitis Control From Science to Practice. 1st Ed, Wageningen Academic Publishers, The Netherlands.
- Roy JP, Coteaux L, Tremblay D, Beaudry F, Elsener J (2009): Efficacy of a 5-day extended therapy program during lactation with cephapirin sodium in dairy cows chronically infected with Staphylococcus aureus. Can Vet J, 50, 1257-1262.
- Sandgren CH, Waller KP, Emanuelson U (2008): The- rapeutic antimicrobial treatment of bovine subclinical mastitis ments with no treatment for bacteriological efficacy during lactation. Vet J, 175, 108-117. against bovine mastitis pathogens. J Dairy Sci, 82, 1664
- Sekkin S, Kum C, Kırkan Ş, Güleş Ö, Akar F (2010): Sütçü ineklerde S. aureus’un neden olduğu subklinik masti- tisin sağaltımında sefoperazon-danofloksasin kombinasyo- nunun etkinliği. Kafkas Univ Vet Fak Derg, 16, 97-104.
- Sol J, Sampimon OC, Snoep JJ, Schukken YH (1997): Factors associated with bacteriological cure during Germany. lactation after therapy for subclinical mastitis caused by Staphylococcus aureus. J Dairy Sci, 80, 2803-2808. Geliş tarihi: 02.05.2014/ Kabul tarihi: 06.11.2014
- Swinkels JM, Cox P, Schukken YH, Lam TJ (2013): Efficacy of extended cefquinome treatment of clinical Staphylococcus aureus mastitis. J Dairy Sci, 96, 4983- 4992.
- Wilson DJ, Gonzalez RN, Case KL, Garrison LL, Grohn YT (1999): Comparison of seven antibiotic treat- 1670.
- Zecconi A, Piccinini R (2002): Intramammary Infections: Epidemiology and Diagnosis. 346-359. In: M Kaske (Ed), Recent Developments and Perspectives in Bovine Medicine. XIIth World Buiatrics Congress, Hannover,
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA94KB72MF |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2015 |
| Published in Issue | Year 2015 |