Abstract
Bu çalışmanın amaçları köpeklerde ovariektomi (OVE) ve ovaryohisterektomi (OVH) ile ilişkili akut faz yanıtının şiddetinin değerlendirilmesi ve paraoksanaz-1 (PON-1) aktivitesinin araştırılmasıydı. Çalışmada toplam 21 adet sağlıklı dişi köpek kullanıldı. Köpekler rastgele OVH (n=11) ve OVE (n=10) yapılmak üzere iki gruba ayrıldı. Operasyon öncesinde ve operasyon sonrası 4. günde alınan periferal kan örneklerinde serum seruloplazmin düzeyi ve paraoksonaz-1 aktivitesi ölçüldü. Sonuçlar gruplar içerisinde ve gruplar arasında karşılaştırıldı. Paraoksanaz-1 ve seruloplazmin arasında muhtemel korelasyon değerlendirildi. Operasyon öncesi serum seruloplazmin seviyeleri OVE ve OVH gruplarında sırasıyla 0.80±0.17 and 1.65±0.35 U/ml olarak ölçüldü (P>0.05). Operasyon sonrası 4. günde serum seruloplazmin seviyesi OVH grubunda (1.95±0.47 U/ml) OVE grubuna kıyasla daha yüksek bulundu (0.69±0.10 U/ml; P<0.05). Operasyon öncesi ve sonrası serum seruloplazmin seviyesi çalışma grupları içerisinde farklılık göstermedi (P>0.05). Operasyon öncesi ve sonrasında serum PON-1 aktivitesi OVE ve OVH gruplarında sırasıyla 1001.56±44.60 U/ml ve 1119.10±87.99 U/ml olup (P>0.05), operasyon sonrası serum PON-1 aktivitesi OVH grubunda (1065.15±46.00 U/ml) OVE grubuna kıyasla daha yüksekti (941.22±32.57 U/ml; P<0.05). PON-1 aktivitesi ile serum seruloplazmin konsantrasyonu arasında bir korelasyon yoktu (P=.250; r=0.06). Sonuç olarak, OVE grubunda postoperatif akut faz yanıtının şiddeti OVH grubuna göre daha düşüktü. Buna ek olarak, köpeklerde OVE ve OVH operasyonu sonrası 4. günde PON-1 aktivitesinde değişiklik ve PON-1 aktivitesi ile serum seruloplazmin konsantrasyonları arasında bir ilişki bulunmadı
References
- Ceron JJ, Eckersall PD, Subiela SM (2005): Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol, 34, 85-99. 2. Ceron JJ, spectrophotometric ceruloplasmin in serum. Vet Res, 35, 671-679. for measuring method canine
- Conner JG, Eckersall PD, Ferguson J, Douglas TA (1988): Acute phase response in the dog following surgical trauma. Res Vet Sci, 45, 107-110.
- De Tora M, McCarty RJ (2011): Ovariohysterectomy versus ovariectomy for elective sterilization of female dogs and cats: is removal of the uterus necessarry?. JAVMA, 239, 1409-1412. 5. Eckersall PD (2008): Proteins, Proteomics and Dysproteinemias. 117-155. In: JJ Kaneko, JW Harvey, ML Bruss, Clinical Biochemistry of Domestic Animals. 6th Ed, Academic Press, San Diego.
- Goethem BV, Schaefers-Okkens A, Kirpensteijn J (2006): Making a rational choice between ovariectomy and ovariohysterectomy in the dog: a discussion of the benefits of either technique. Veterinary Surgery, 35, 136- 143.
- Gunay A, Gunes N, Gunay U (2011): Effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and biochemical parameters in bitches. Bull Vet Inst Pulawy, 55, 695-698.
- Kirpensteijn J (2008): Ovariectomy versus ovariohysterectomy. Is the eternal argument ended. Proceedings of the international SCIVAC Congress, Rimini, Italy, 290-293.
- Kum C, Voyvoda H, Sekkin S, Karademir U, Tarimcilar T (2013): Effects of carprofen and meloxicam on C-reactive protein, ceruloplasmin, and fibrinogen concentrations in dogs undergoing ovariohysterectomy. Am J Vet Res, 74, 1267-1273.
- Lee JY, Kim MC (2014): Comparison of oxidative stress status in dogs undergoing laparoscopic and open ovariectomy. J Vet Med Sci, 76, 273-276.
- Rossi G, Giordano A, Pezzia F, Kjelgaard-Hansen M, Paltrinieri S (2013): Serum paraoxonase 1 activity in dogs: preanalytical and analytical factors and correlation with C-reactive protein and alpha-2-globulin. Vet Clin Pathol, 42, 329-341.
- Serin G, Kiral F, Serin I (2008): Acute effect of ovariohysterectomy on lipid peroxidation and some antioxidant levels in dogs. Bull Vet Inst Pulawy, 52, 251- 253.
- Sunderman FW, Nomoto S (1970): Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem, 16, 903-910.
Abstract
The objectives of this study were to evaluate the severity of acute phase reaction and to investigate the paraoxanase-1 (PON-1) activity associated with ovariectomy (OVE) and ovariohysterectomy (OVH) in dogs. A total of 21 adult healthy dogs were included. The dogs were randomly divided into two groups and either ovariectomized (n=10) or ovariohysterectomized (n=11). Peripheral blood samples were collected preoperatively and on postoperative fourth day for serum ceruloplasmin (cp) and paraoxanase-1 activity measurements. Results were compared within and between groups. Potential correlation between paraoxanase-1 and ceruloplasmin was assessed. Preoperative mean serum ceruloplasmin levels in OVE and OVH groups were 0.80±0.17 and 1.65±0.35 U/ml, respectively (P>0.05). On postoperative 4th day, serum ceruloplasmin levels in OVH group (1.95±0.47 U/ml) was higher compared to OVE group (0.69±0.10 U/ml; P<0.05). Pre- and postoperative serum ceruloplasmin levels were not different within study groups (P>0.05). Preoperative serum paraoxanase-1 activity in OVE and OVH groups were 1001.56±44.60 U/ml and 1119.10±87.99 U/ml, respectively (P>0.05). Higher postoperative serum paraoxanase-1 activity was observed in OVH group (1065.15±46.00 U/ml) compared to OVE group (941.22±32.57 U/ml; P<0.05). Pre and postoperative serum paraoxanase-1 activity was not different within study groups (P>0.05). Paraoxanase-1 activity was not correlated with concentration of ceruloplasmin (P=.250; r=0.06). In conclusion, OVE is superior to OVH in terms of reducing the severity of postoperative acute phase response. In addition, OVE and OVH does not alter canine paraoxanase-1 activity on postoperative fourth day and paraoxanase-1 activity is not associated with serum concentration of ceruloplasmin in dogs
References
- Ceron JJ, Eckersall PD, Subiela SM (2005): Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol, 34, 85-99. 2. Ceron JJ, spectrophotometric ceruloplasmin in serum. Vet Res, 35, 671-679. for measuring method canine
- Conner JG, Eckersall PD, Ferguson J, Douglas TA (1988): Acute phase response in the dog following surgical trauma. Res Vet Sci, 45, 107-110.
- De Tora M, McCarty RJ (2011): Ovariohysterectomy versus ovariectomy for elective sterilization of female dogs and cats: is removal of the uterus necessarry?. JAVMA, 239, 1409-1412. 5. Eckersall PD (2008): Proteins, Proteomics and Dysproteinemias. 117-155. In: JJ Kaneko, JW Harvey, ML Bruss, Clinical Biochemistry of Domestic Animals. 6th Ed, Academic Press, San Diego.
- Goethem BV, Schaefers-Okkens A, Kirpensteijn J (2006): Making a rational choice between ovariectomy and ovariohysterectomy in the dog: a discussion of the benefits of either technique. Veterinary Surgery, 35, 136- 143.
- Gunay A, Gunes N, Gunay U (2011): Effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and biochemical parameters in bitches. Bull Vet Inst Pulawy, 55, 695-698.
- Kirpensteijn J (2008): Ovariectomy versus ovariohysterectomy. Is the eternal argument ended. Proceedings of the international SCIVAC Congress, Rimini, Italy, 290-293.
- Kum C, Voyvoda H, Sekkin S, Karademir U, Tarimcilar T (2013): Effects of carprofen and meloxicam on C-reactive protein, ceruloplasmin, and fibrinogen concentrations in dogs undergoing ovariohysterectomy. Am J Vet Res, 74, 1267-1273.
- Lee JY, Kim MC (2014): Comparison of oxidative stress status in dogs undergoing laparoscopic and open ovariectomy. J Vet Med Sci, 76, 273-276.
- Rossi G, Giordano A, Pezzia F, Kjelgaard-Hansen M, Paltrinieri S (2013): Serum paraoxonase 1 activity in dogs: preanalytical and analytical factors and correlation with C-reactive protein and alpha-2-globulin. Vet Clin Pathol, 42, 329-341.
- Serin G, Kiral F, Serin I (2008): Acute effect of ovariohysterectomy on lipid peroxidation and some antioxidant levels in dogs. Bull Vet Inst Pulawy, 52, 251- 253.
- Sunderman FW, Nomoto S (1970): Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem, 16, 903-910.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA73AV75TN |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2015 |
| Published in Issue | Year 2015 |