Antimicrobial susceptibility and serotype distribution of Listeriamonocytogenes isolates obtained from raw milk cheese samples sold inNigde
Abstract
This study was designed to evaluate public health risks in respect to listeriosis due to consumption of local raw milk cheese sold in villages and public bazaars of Nigde. In addition, this study aims to contribute to the treatment process of listeriosis by determining serotype distribution and antimicrobial susceptibility profiles of the isolates. In the study, two of cheese (1 %) samples of total 200 raw milk cheese samples (100 white cheeses and 100 tulum cheeses) were found contamined with L. monocytogenes. Serotype distribution of the isolates were determined as 1/2a (white cheese isolate) and 4a (tulum cheese isolate). Finally, antimicrobial resistance profiles of the isolates against 11 antibiotics (tetracycline, ciprofloxacin, gentamicin, ampicillin, erythromycin, trimethoprim, vancomycin, chloramphenicol, nalidixic acid, penicillin G and sulphanilamide) were determined and the MIC values were specified by a microdilution method. Both of the isolates were found to be resistant to nalidixic acid but sensitive to the others analysed. As a result, although low L. monocytogenes prevalance rates were detected in this study, it is a public health concern to isolate serotype 1/2a which is secondly incriminated serotype to cause human listeriosis. In addition, it is important in terms of public health to continue surveillance of emerging antimicrobial resistance among L. monocytogenes isolated especially from foods of animal origin though incidence of antibiotic resistance in L. monocytogenes isolates is rare
Keywords
Antimicrobial susceptibility cheese Listeria monocytogenes microdilution serotype distribution
References
- Arslan S, Ozdemir F (2008). Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control 19: 360– 363.
- Bagcı C, Cinar M (2005). Isolation and idenlifiealion of Listeria monocytogenes from white pickled cheese consumed in Niğde region. Vet Bil Derg 21: 69- 74.
- Border P, Howard J, Plastow G, Siggens K (1990). Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Lett Appl Microbiol 11: 158- 162.
- Buyukoruk S, Goksoy EO (2011). Investigation the presence of Listeria spp. from village cheese in Aydın province. Uludag Univ J Fac Vet Med 30: 9- 12.
- CLSI (2008). Development of In vitro Susceptibility Testing Criteria and Quality Control Parameters for Veterinary Antimicrobial Agents—Third Edition: Approved Guideline M37-A3., 28, 8. and Informational Supplement M31- A2, 2004; 24, 17. Clinical and Laboratory Standards Institute.
- Filiousis G, Johansson A, Frey J, Perreten V (2009). Prevalence, genetic diversity and antimicrobial susceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control 20: 314– 317.
- Gyles CL (2008). Antimicrobial resistance in selected bacteria from poultry. Anim Health Res Rev 9: 149- 158.
- ISO (2004). Horizontal Method for Detection of Listeria monocytogenes International Standardization Organization ISO NORM, 11290-1-/A1.
- Jajali M, Abedi D (2008). Prevalence of Listeria species in food products in Isfahan, Iran. Int J Food Microbiol 122: 336–340.
- Korsak D, Borek A, Daniluk S, Grabowska A, Pappelbaum K (2012). Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland. Int J Food Microbiol 158: 203– 208.
- Kum E, Yildirim Y, Ertas N (2011). Detection of Listeria monocytogenes in cheese samples retailed in Kayseri by classical cultural technique. J Fac Vet Med Univ Erciyes 8: 105 -109.
- Liu D, Lawrence M, Wiedmann M, Gorski L, Mandrell RE, Ainsworth AJ, Austin FW (2006) Listeria monocytogenes subgroups IIIA, IIIB, and IIIC delineate genetically distinct populations with varied pathogenic potential. J Clin Microbiol 44: 4229– 4233.
- Manfreda G, De Cesare A, Stella S, Cozzi M, Cantoni C (2005). Occurrence and ribotypes of Listeria monocytogenes in Gorgonzola cheeses. Int J Food Microbiol 102: 287– 293.
- McLauchlin J, Mitchell RT, Smerdon WJ, Jewell K (2004). Listeria monocytogenes and listeriosis: a review of hazard characterisation for use in microbiological risk assessment of foods. Int J Food Microbiol 92: 15– 33.
- Navratilova P, Schlegelova J, Sustackova A (2004). Prevalence of Listeria monocytogenes in milk, meat and food stuff of animal origin and the phenotype of antibiotic resistance of isolated strains. Vet Med Czech 49: 243–252.
- Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M (2004). Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol 70: 4458– 4467.
- O’Connor L, O’Leary M, Leonard N, Godinho M, O'Reilly C, Coffey L, Egan J, O'Mahony R (2010). The characterization of Listeria spp. isolated from food products and the food-processing environment. Lett Appl Microbiol 51: 490– 498.
- Orsi RH, Bakker CH, Wiedmann M (2011). Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol 301: 79- 96.
- Pourjafar M, Badiei K, Oryan A, Tabatabei M, Ghane M, Ahmadi N (2010). Clinico-pathological, bacteriological and PCR findings of Ovine Listeriosis: an emerging disease in southern Iran. Global Vet 5: 226- 232.
- Poyart-Salmeron C, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P (1990). Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet 335: 1422–1426.
- Rahimi E, Ameri M, Momtaz H (2010). Prevalence and antimicrobial resistance of Listeria species isolated from milk and dairy products in Iran. Food Control 21: 1448- 1452.
- Ruiz-Bolivar Z, Neuque-Rico MC, Poutou-Pinales RA, Carrascal-Camaco AK, Mattar S (2011). Antimicrobial susceptibility of Listeria monocytogenes isolated from different cities in Colombia. Foodborne Pathog Dis 8: 913- 919.
- Sleator RD, Francis GA, O’Beirne D, Gahan CGM, Hill C (2003). Betaine and carnitine uptake systems in Listeria monocytogenes affect growth and survival in foods and during infection. J Appl Microbiol 95: 839- 846.
- Troxler R, von Gravenitz A, Funke G, Wiedemann B, Stock I (2000). Natural antibiotic susceptibility of Listeria species: L. grayi, L. innocua, L. ivanovii, L. monocytogenes, L. seeligerii and L. welshimerii strains. Clin Microbiol Infec 6: 525– 535.
- Ward TJ, Gorski L, Borucki MK, Mandrell RE, Hutchins J, Pupedis K (2004). Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes. J Bacteriol 186: 4994–5002.
- Yan H, Neogi SB, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L, Zhong N (2010). Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005–2007. Int J Food Microbiol 144: 310- 316.
- Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA, Meng J (2007). Characterization of Listeria monocytogenes isolated from retail foods. Int J Food Microbiol 113: 47- 53.
- Zhang D, Zhang L, Wang D, Suo B, Shi X (2010). A PCR method for the detection of Listeria monocytogenes based on a novel target sequence identified by comparative genomic analysis. J Food Safety 30: 832–847.
- Zeng H, Zhang X, Sun Z, Fang W (2006). Multiplex PCR identification of Listeria monocytogenes isolates from milk and milk-processing environments. J Sci Food Agric 86: 367– 371.
Niğde’de satışa sunulan çiğ sütten yapılmış peynir örneklerinden elde edilen Listeria monocytogenesizolatlarının serotip dağılımının ve antimikrobiyel duyarlılık profilinin belirlenmesi
Abstract
Bu çalışma, Niğde ili köy ve pazarlarında tüketime sunulan çiğ sütten yapılmış peynirlerin listeriozis açısından taşıdığı halk sağlığı risklerini değerlendirmek ve peynirlerden elde edilen izolatların serotip dağılımını ve antimikrobiyel duyarlılık profillerini belirleyerek tedavi sürecine katkıda bulunmak amacıyla planlanmıştır. Çalışma sonucunda 200 çiğ sütten yapılmış peynir örneğinden (100 beyaz peynir ve 100 tulum peyniri) ikisinin (% 1) L. monocytogenes ile kontamine olduğu bulunmuştur. L. monocytogenes izolatlarının serotip dağılımı 1/2a (beyaz peynir izolatı) ve 4a (tulum peyniri izolatı) olarak belirlenmiştir. Son olarak izolatların 11 antibiyotiğe (tetrasiklin, siprofloksasin, gentamisin, ampisilin, eritromisin, trimetoprim, vankomisin, kloramfenikol, nalidiksik asit, penisilin G ve sülfonilamid) karşı direnç profilleri belirlenmiş ve MIC değerleri mikrodilüsyon metodu ile ortaya konmuştur. İzolatların her ikisinin de nalidiksik asite dirençli, diğer antibiyotiklere duyarlı olduğu tespit edilmiştir. Sonuç olarak incelenen örneklerde L. monocytogenes prevalansının çok düşük düzeylerde bulunmasına karşın insan listeriozisinden ikinci sırada sorumlu tutulan 1/2a serotipinin belirlenmiş olması halk sağlığı açısından risk unsuru olarak değerlendirilmiştir. Ayrıca, L. monocytogenes izolatları arasındaki antimikrobiyel dirençlilik nadir olsa da özellikle hayvansal gıdalardan elde edilen L. monocytogenes izolatlarının antimikrobiyel direnç profillerinin sürekli gözetim altında tutulması halk sağlığı açısından önem arzetmektedir
References
- Arslan S, Ozdemir F (2008). Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control 19: 360– 363.
- Bagcı C, Cinar M (2005). Isolation and idenlifiealion of Listeria monocytogenes from white pickled cheese consumed in Niğde region. Vet Bil Derg 21: 69- 74.
- Border P, Howard J, Plastow G, Siggens K (1990). Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Lett Appl Microbiol 11: 158- 162.
- Buyukoruk S, Goksoy EO (2011). Investigation the presence of Listeria spp. from village cheese in Aydın province. Uludag Univ J Fac Vet Med 30: 9- 12.
- CLSI (2008). Development of In vitro Susceptibility Testing Criteria and Quality Control Parameters for Veterinary Antimicrobial Agents—Third Edition: Approved Guideline M37-A3., 28, 8. and Informational Supplement M31- A2, 2004; 24, 17. Clinical and Laboratory Standards Institute.
- Filiousis G, Johansson A, Frey J, Perreten V (2009). Prevalence, genetic diversity and antimicrobial susceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control 20: 314– 317.
- Gyles CL (2008). Antimicrobial resistance in selected bacteria from poultry. Anim Health Res Rev 9: 149- 158.
- ISO (2004). Horizontal Method for Detection of Listeria monocytogenes International Standardization Organization ISO NORM, 11290-1-/A1.
- Jajali M, Abedi D (2008). Prevalence of Listeria species in food products in Isfahan, Iran. Int J Food Microbiol 122: 336–340.
- Korsak D, Borek A, Daniluk S, Grabowska A, Pappelbaum K (2012). Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland. Int J Food Microbiol 158: 203– 208.
- Kum E, Yildirim Y, Ertas N (2011). Detection of Listeria monocytogenes in cheese samples retailed in Kayseri by classical cultural technique. J Fac Vet Med Univ Erciyes 8: 105 -109.
- Liu D, Lawrence M, Wiedmann M, Gorski L, Mandrell RE, Ainsworth AJ, Austin FW (2006) Listeria monocytogenes subgroups IIIA, IIIB, and IIIC delineate genetically distinct populations with varied pathogenic potential. J Clin Microbiol 44: 4229– 4233.
- Manfreda G, De Cesare A, Stella S, Cozzi M, Cantoni C (2005). Occurrence and ribotypes of Listeria monocytogenes in Gorgonzola cheeses. Int J Food Microbiol 102: 287– 293.
- McLauchlin J, Mitchell RT, Smerdon WJ, Jewell K (2004). Listeria monocytogenes and listeriosis: a review of hazard characterisation for use in microbiological risk assessment of foods. Int J Food Microbiol 92: 15– 33.
- Navratilova P, Schlegelova J, Sustackova A (2004). Prevalence of Listeria monocytogenes in milk, meat and food stuff of animal origin and the phenotype of antibiotic resistance of isolated strains. Vet Med Czech 49: 243–252.
- Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M (2004). Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol 70: 4458– 4467.
- O’Connor L, O’Leary M, Leonard N, Godinho M, O'Reilly C, Coffey L, Egan J, O'Mahony R (2010). The characterization of Listeria spp. isolated from food products and the food-processing environment. Lett Appl Microbiol 51: 490– 498.
- Orsi RH, Bakker CH, Wiedmann M (2011). Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol 301: 79- 96.
- Pourjafar M, Badiei K, Oryan A, Tabatabei M, Ghane M, Ahmadi N (2010). Clinico-pathological, bacteriological and PCR findings of Ovine Listeriosis: an emerging disease in southern Iran. Global Vet 5: 226- 232.
- Poyart-Salmeron C, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P (1990). Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet 335: 1422–1426.
- Rahimi E, Ameri M, Momtaz H (2010). Prevalence and antimicrobial resistance of Listeria species isolated from milk and dairy products in Iran. Food Control 21: 1448- 1452.
- Ruiz-Bolivar Z, Neuque-Rico MC, Poutou-Pinales RA, Carrascal-Camaco AK, Mattar S (2011). Antimicrobial susceptibility of Listeria monocytogenes isolated from different cities in Colombia. Foodborne Pathog Dis 8: 913- 919.
- Sleator RD, Francis GA, O’Beirne D, Gahan CGM, Hill C (2003). Betaine and carnitine uptake systems in Listeria monocytogenes affect growth and survival in foods and during infection. J Appl Microbiol 95: 839- 846.
- Troxler R, von Gravenitz A, Funke G, Wiedemann B, Stock I (2000). Natural antibiotic susceptibility of Listeria species: L. grayi, L. innocua, L. ivanovii, L. monocytogenes, L. seeligerii and L. welshimerii strains. Clin Microbiol Infec 6: 525– 535.
- Ward TJ, Gorski L, Borucki MK, Mandrell RE, Hutchins J, Pupedis K (2004). Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes. J Bacteriol 186: 4994–5002.
- Yan H, Neogi SB, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L, Zhong N (2010). Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005–2007. Int J Food Microbiol 144: 310- 316.
- Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA, Meng J (2007). Characterization of Listeria monocytogenes isolated from retail foods. Int J Food Microbiol 113: 47- 53.
- Zhang D, Zhang L, Wang D, Suo B, Shi X (2010). A PCR method for the detection of Listeria monocytogenes based on a novel target sequence identified by comparative genomic analysis. J Food Safety 30: 832–847.
- Zeng H, Zhang X, Sun Z, Fang W (2006). Multiplex PCR identification of Listeria monocytogenes isolates from milk and milk-processing environments. J Sci Food Agric 86: 367– 371.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA26PM54PG |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2014 |
| Published in Issue | Year 2014 |