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Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey

Year 2013, , 27 - 31, 01.03.2013
https://doi.org/10.1501/Vetfak_0000002549

Abstract

In this study, infectious bronchitis (IB)-suspect problems were observed in 2 broiler and 8 breeder chicken flocks vaccinated with H120 strain, and a total of 220 tracheal swabs were taken from 60 broiler chickens of 2 flocks (30 swabs/flock), and 160 breeder chickens of 8 flocks (20 swabs/flock) to detect infectious bronchitis virus (IBV) by a reverse transcriptase polymerase chain reaction (RT-PCR) and to isolate IBV from chickens showing respiratory sings and nephritis lesions. Detection of 8 Turkish IBVs from the tracheal swab samples directly by RT-PCR was confirmed by both IBV isolation from allantoic fluids of embryonated eggs and by the same RT-PCRs from these samples. Basic Local Alignment Search Tool (BLAST) results revealed that all 8 sequences from our Turkish IBV isolates were related to that of EU780077 (IS/1494/06 IBV strain) with a 99% identity. Phylogenetic and alignment analyses of the sequences of two representative isolates as TR_IS1494_06_B12011 from one broiler flock and TR_1494_06_7201 from one breeder flock also confirmed this close relation to IS/1494/06 IBV genotype. As a result, Turkish IBVs have been isolated for the first time from chickens, which were not protected from IB despite IBV H120 strain vaccination, and genotyped as IS/1494/06 in Turkey suggesting the transfer of this IBV from the countries of Middle East

References

  • Bochkov YA, Batchenko GV, Shcherbakova LO, Borisov AV, Drygin VV (2006): Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathol, 35, 379–393.
  • Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987): Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol, 68, 57–77.
  • Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME, Brown TD (1986): Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res, 4, 133–143.
  • Cavanagh D, Davis PJ, Cook J (1992): Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401–408.
  • Cavanagh D (1997): Nidovirales: A new order comprising Coronoviridae and Arteriviridae. Arch Virol, 142,629– 633.
  • Cook JKA, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
  • De Wit JJ, Cook JKA, van der Heijden HMJF (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
  • Gelb J, Weisman Y, Ladman BS, Meir R (2005): S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol, 34, 194– 203.
  • Ignjatovic J, Sapats S (2000): Avian infectious bronchitis virus. The OIE Scientific and Technical Review, 19, 493– 508.
  • Kingham BF, Keeler CL Jr, Nix WA, Ladman BS, Gelb J Jr (2000): Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis, 44,325–335.
  • Kottier SA, Cavanagh D, Britton P (1995): Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology, 213, 569–580.
  • Lee CW, Jackwood MW (2000): Evidence of genetic diversity generated by recombination among avian coronovirus IBV. Arch Virol, 145, 2135–2148.
  • Mahmood ZH, Sleman RR Uthman AU (2011): Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol, 150, 21–27.
  • OIE Terrestrial Manual 2008. Chapter 2.3.2. (2008): Avian Infectious Bronchitis. pp. 443–455.
  • Pohuang T, Chansiripornchai N, Tawatsin A,
  • Sasipreeyajan J (2009): Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 10, 219–223.

Türkiye’de damızlık ve broyler sürülerde IS/1494/06 genotipi-ilişkili infeksiyöz bronşit virusununvarlığı

Year 2013, , 27 - 31, 01.03.2013
https://doi.org/10.1501/Vetfak_0000002549

Abstract

Bu çalışmada H120 suşu ile aşılanmış 2 broyler ve 8 damızlık sürüde infeksiyöz bronşit şüpheli problemlere rastlanmış 2 sürüye ait 60 broyler tavuktan (30 svap/sürü) ve 8 sürüye ait 160 damızlık tavuktan (20 svap/sürü) özellikle solunum bulguları ile nefrit lezyonları gösterenlerden toplam 220 trakeal svap infeksiyöz bronşit virusu (IBV)’nu reverse transcriptase polymerase chain reaction (RT-PCR) ile belirlemek ve IBV’yi izole etmek amacı ile alındı. IBV’nin trakeal svap örneklerinden direkt olarak RT-PCR ile deteksiyonu embryolu yumurtaların allantoik sıvılarından IBV izolasyonu ve bu örneklerden RT-PCR yapılması ile doğrulandı. Basic Local Alignment Search Tool (BLAST) sonuçları 8 Türk izolatının EU780077 (IS/1494/06 IBV suşu) ile %99 benzerlik gösterdiğini ortaya koydu. Broyler sürüden TR_IS1494_06_B12011 ve damızlık sürüden TR_1494_06_7201 olarak seçilen temsili iki izolatın dizilerinin filogenetik ve sıralama analizleri de IS/1494/06 IBV genotipine yakınlığı doğruladı. Sonuç olarak, Türkiye’de IBV H120 suşu ile aşılamaya rağmen IB’den korunamayan tavuklardan ilk defa izole edilmesi ve IS/1494/06 olarak genotiplendirilmesi bu IBV’nin Orta Doğu ülkelerinden taşınma olasılığını akla getirmektedir

References

  • Bochkov YA, Batchenko GV, Shcherbakova LO, Borisov AV, Drygin VV (2006): Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathol, 35, 379–393.
  • Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987): Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol, 68, 57–77.
  • Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME, Brown TD (1986): Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res, 4, 133–143.
  • Cavanagh D, Davis PJ, Cook J (1992): Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401–408.
  • Cavanagh D (1997): Nidovirales: A new order comprising Coronoviridae and Arteriviridae. Arch Virol, 142,629– 633.
  • Cook JKA, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
  • De Wit JJ, Cook JKA, van der Heijden HMJF (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
  • Gelb J, Weisman Y, Ladman BS, Meir R (2005): S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol, 34, 194– 203.
  • Ignjatovic J, Sapats S (2000): Avian infectious bronchitis virus. The OIE Scientific and Technical Review, 19, 493– 508.
  • Kingham BF, Keeler CL Jr, Nix WA, Ladman BS, Gelb J Jr (2000): Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis, 44,325–335.
  • Kottier SA, Cavanagh D, Britton P (1995): Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology, 213, 569–580.
  • Lee CW, Jackwood MW (2000): Evidence of genetic diversity generated by recombination among avian coronovirus IBV. Arch Virol, 145, 2135–2148.
  • Mahmood ZH, Sleman RR Uthman AU (2011): Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol, 150, 21–27.
  • OIE Terrestrial Manual 2008. Chapter 2.3.2. (2008): Avian Infectious Bronchitis. pp. 443–455.
  • Pohuang T, Chansiripornchai N, Tawatsin A,
  • Sasipreeyajan J (2009): Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 10, 219–223.
There are 16 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA24JG33SA
Journal Section Research Article
Authors

Serpil Kahya

Fethiye Coven

Seran Temellı

Aysegul Eyıgor

Kamil Tayfun Carlı

Publication Date March 1, 2013
Published in Issue Year 2013

Cite

APA Kahya, S., Coven, F., Temellı, S., Eyıgor, A., et al. (2013). Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60(1), 27-31. https://doi.org/10.1501/Vetfak_0000002549
AMA Kahya S, Coven F, Temellı S, Eyıgor A, Carlı KT. Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg. March 2013;60(1):27-31. doi:10.1501/Vetfak_0000002549
Chicago Kahya, Serpil, Fethiye Coven, Seran Temellı, Aysegul Eyıgor, and Kamil Tayfun Carlı. “Presence of IS/1494/06 Genotype-Related Infectious Bronchitis Virus in Breeder and Broiler Flocks in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60, no. 1 (March 2013): 27-31. https://doi.org/10.1501/Vetfak_0000002549.
EndNote Kahya S, Coven F, Temellı S, Eyıgor A, Carlı KT (March 1, 2013) Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 1 27–31.
IEEE S. Kahya, F. Coven, S. Temellı, A. Eyıgor, and K. T. Carlı, “Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey”, Ankara Univ Vet Fak Derg, vol. 60, no. 1, pp. 27–31, 2013, doi: 10.1501/Vetfak_0000002549.
ISNAD Kahya, Serpil et al. “Presence of IS/1494/06 Genotype-Related Infectious Bronchitis Virus in Breeder and Broiler Flocks in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60/1 (March 2013), 27-31. https://doi.org/10.1501/Vetfak_0000002549.
JAMA Kahya S, Coven F, Temellı S, Eyıgor A, Carlı KT. Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg. 2013;60:27–31.
MLA Kahya, Serpil et al. “Presence of IS/1494/06 Genotype-Related Infectious Bronchitis Virus in Breeder and Broiler Flocks in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 60, no. 1, 2013, pp. 27-31, doi:10.1501/Vetfak_0000002549.
Vancouver Kahya S, Coven F, Temellı S, Eyıgor A, Carlı KT. Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg. 2013;60(1):27-31.

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