Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey
Abstract
In this study, infectious bronchitis (IB)-suspect problems were observed in 2 broiler and 8 breeder chicken flocks vaccinated with H120 strain, and a total of 220 tracheal swabs were taken from 60 broiler chickens of 2 flocks (30 swabs/flock), and 160 breeder chickens of 8 flocks (20 swabs/flock) to detect infectious bronchitis virus (IBV) by a reverse transcriptase polymerase chain reaction (RT-PCR) and to isolate IBV from chickens showing respiratory sings and nephritis lesions. Detection of 8 Turkish IBVs from the tracheal swab samples directly by RT-PCR was confirmed by both IBV isolation from allantoic fluids of embryonated eggs and by the same RT-PCRs from these samples. Basic Local Alignment Search Tool (BLAST) results revealed that all 8 sequences from our Turkish IBV isolates were related to that of EU780077 (IS/1494/06 IBV strain) with a 99% identity. Phylogenetic and alignment analyses of the sequences of two representative isolates as TR_IS1494_06_B12011 from one broiler flock and TR_1494_06_7201 from one breeder flock also confirmed this close relation to IS/1494/06 IBV genotype. As a result, Turkish IBVs have been isolated for the first time from chickens, which were not protected from IB despite IBV H120 strain vaccination, and genotyped as IS/1494/06 in Turkey suggesting the transfer of this IBV from the countries of Middle East
References
- Bochkov YA, Batchenko GV, Shcherbakova LO, Borisov AV, Drygin VV (2006): Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathol, 35, 379–393.
- Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987): Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol, 68, 57–77.
- Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME, Brown TD (1986): Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res, 4, 133–143.
- Cavanagh D, Davis PJ, Cook J (1992): Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401–408.
- Cavanagh D (1997): Nidovirales: A new order comprising Coronoviridae and Arteriviridae. Arch Virol, 142,629– 633.
- Cook JKA, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
- De Wit JJ, Cook JKA, van der Heijden HMJF (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
- Gelb J, Weisman Y, Ladman BS, Meir R (2005): S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol, 34, 194– 203.
- Ignjatovic J, Sapats S (2000): Avian infectious bronchitis virus. The OIE Scientific and Technical Review, 19, 493– 508.
- Kingham BF, Keeler CL Jr, Nix WA, Ladman BS, Gelb J Jr (2000): Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis, 44,325–335.
- Kottier SA, Cavanagh D, Britton P (1995): Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology, 213, 569–580.
- Lee CW, Jackwood MW (2000): Evidence of genetic diversity generated by recombination among avian coronovirus IBV. Arch Virol, 145, 2135–2148.
- Mahmood ZH, Sleman RR Uthman AU (2011): Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol, 150, 21–27.
- OIE Terrestrial Manual 2008. Chapter 2.3.2. (2008): Avian Infectious Bronchitis. pp. 443–455.
- Pohuang T, Chansiripornchai N, Tawatsin A,
- Sasipreeyajan J (2009): Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 10, 219–223.
Türkiye’de damızlık ve broyler sürülerde IS/1494/06 genotipi-ilişkili infeksiyöz bronşit virusununvarlığı
Abstract
Bu çalışmada H120 suşu ile aşılanmış 2 broyler ve 8 damızlık sürüde infeksiyöz bronşit şüpheli problemlere rastlanmış 2 sürüye ait 60 broyler tavuktan (30 svap/sürü) ve 8 sürüye ait 160 damızlık tavuktan (20 svap/sürü) özellikle solunum bulguları ile nefrit lezyonları gösterenlerden toplam 220 trakeal svap infeksiyöz bronşit virusu (IBV)’nu reverse transcriptase polymerase chain reaction (RT-PCR) ile belirlemek ve IBV’yi izole etmek amacı ile alındı. IBV’nin trakeal svap örneklerinden direkt olarak RT-PCR ile deteksiyonu embryolu yumurtaların allantoik sıvılarından IBV izolasyonu ve bu örneklerden RT-PCR yapılması ile doğrulandı. Basic Local Alignment Search Tool (BLAST) sonuçları 8 Türk izolatının EU780077 (IS/1494/06 IBV suşu) ile %99 benzerlik gösterdiğini ortaya koydu. Broyler sürüden TR_IS1494_06_B12011 ve damızlık sürüden TR_1494_06_7201 olarak seçilen temsili iki izolatın dizilerinin filogenetik ve sıralama analizleri de IS/1494/06 IBV genotipine yakınlığı doğruladı. Sonuç olarak, Türkiye’de IBV H120 suşu ile aşılamaya rağmen IB’den korunamayan tavuklardan ilk defa izole edilmesi ve IS/1494/06 olarak genotiplendirilmesi bu IBV’nin Orta Doğu ülkelerinden taşınma olasılığını akla getirmektedir
References
- Bochkov YA, Batchenko GV, Shcherbakova LO, Borisov AV, Drygin VV (2006): Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathol, 35, 379–393.
- Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987): Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol, 68, 57–77.
- Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME, Brown TD (1986): Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res, 4, 133–143.
- Cavanagh D, Davis PJ, Cook J (1992): Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401–408.
- Cavanagh D (1997): Nidovirales: A new order comprising Coronoviridae and Arteriviridae. Arch Virol, 142,629– 633.
- Cook JKA, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
- De Wit JJ, Cook JKA, van der Heijden HMJF (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
- Gelb J, Weisman Y, Ladman BS, Meir R (2005): S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol, 34, 194– 203.
- Ignjatovic J, Sapats S (2000): Avian infectious bronchitis virus. The OIE Scientific and Technical Review, 19, 493– 508.
- Kingham BF, Keeler CL Jr, Nix WA, Ladman BS, Gelb J Jr (2000): Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis, 44,325–335.
- Kottier SA, Cavanagh D, Britton P (1995): Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology, 213, 569–580.
- Lee CW, Jackwood MW (2000): Evidence of genetic diversity generated by recombination among avian coronovirus IBV. Arch Virol, 145, 2135–2148.
- Mahmood ZH, Sleman RR Uthman AU (2011): Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol, 150, 21–27.
- OIE Terrestrial Manual 2008. Chapter 2.3.2. (2008): Avian Infectious Bronchitis. pp. 443–455.
- Pohuang T, Chansiripornchai N, Tawatsin A,
- Sasipreeyajan J (2009): Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 10, 219–223.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA24JG33SA |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2013 |
| Published in Issue | Year 2013 |