Research Article
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Year 2019, , 373 - 378, 09.09.2019

Zerrin Erginkaya , Hatice Uludağ Emel Ünal Turhan

https://doi.org/10.33988/auvfd.543426

Abstract

References

  • 1. Achemchem F, Bueno MM, Abrini J et al (2005): Enterococcus faecium F58, a bacteriocinogenic strain naturally occurring in Jben, a soft, farm house goat’s cheese made in Morocco. J Appl Microbiol, 99, 141-15.
  • 2. Anandani JH, Khan ZH (2014): Isolation, partial purification and biochemical characterization of enterocin producing enterococci. Indian J Appl Res, 4, 18-20.
  • 3. Aroujo TF, Ferreira LLF (2013): The genus Enterococcus as probiotic: Safety concerns. Braz Arch Biol Techn, 56, 457-466.
  • 4. Belicová A, Mikulášová M, Krajčovič J et al (2011): Antibacterial activity and enterocin genes in enterococci isolated from Bryndza cheese. J Food Nutr Res, 50, 21–32.
  • 5. Belguesmia Y, Choiset Y, Pr´evost H et al (2010): Partial purification and characterization of the mode of action of enterocin s37:a bacteriocin produced by Enterococcus faecalis S37 isolated from poultry feces. J Environ Pub Health, 986460, 1-8.
  • 6. Cisneros YMA, Espuñes TRS, Wacher C et al (2011): Enterocins: Bacteriocins with Applications in the Food Industry. 1330-1341. In: A. Méndez-Vilas (Ed), Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, Microbiology Series (No 3). Formatex, Badajoz, Spain.
  • 7. De Vuyst L, Morenoa MRF Revets H (2003): Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int J Food Microbiol, 84, 299-318.
  • 8. Franz CMAP, Toit MD, Holy A et al (1997): Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. J Basic Microbiol, 37, 187-196.
  • 9. Ghrairi T, Free J, Berjeaud JM et al (2008): Purification and characterization of bacteriocin produced by Enterococcus faecium from Tunisian Rigouta Cheese. Food Control, 19, 162-169.
  • 10. Gómez NC, Abriouel H, Grande MJ et al (2012): Effect of enterocin AS-8 in combination with biocides on planktonic and sessile Listeria monocytogenes. Food Microbiol, 30, 51-58.
  • 11. Isleroglu H, Yildirim Z, Tokatli M (2012): Partial characterization of enterocin KP produced by Enterococcus faecalis KP, a cheese isolate. Int J Dairy Techn, 65, 90-97.
  • 12. Javed A, Masud T, Ain Q et al (2011): Enterocins of Enterococcus faecium, emerging natural food preservatives. Ann Microbiol, 61, 699-708.
  • 13. Javed I, Ali MI, Ahmed B, et al (2011): Optimization and partial purification of bacteriocins from Enterococcus spp. indigenous to Pakistan. Food Biotechn, 25, 130-139.
  • 14. Khalkhali S, Mojgani N (2017): Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk. Iranian J Microbiol, 9, 224-233.
  • 15. Marekova M, Laukova A, De Vuyst L, et al (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J Appl Microbiol, 94, 523-530.
  • 16. Moreno MRF, Callewaert R, Devreese B, et al (2003): Isolation and biochemical characterization of enterocins produced by enterococci from different sources. J Applied Microbiol, 94, 214-229.
  • 17. Nascimento MS, Moreno I, Kuaye AY (2010): Antimicrobial activity of Enterococcus faecium fair-E 198 against Gram-Positive pathogens. Braz J Microbiol, 41, 74-81.
  • 18. Ogaki MB, Rocha KR, Terra MR, et al (2016): Screening of the enterocin-encoding genes and antimicrobial activity in Enterococcus species. J Microbiol Biotechn., 26, 1026-1034.
  • 19. Özdemir GB, Oryaşın E, Bıyık HH, et al (2011): Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian J Microbiol, 51, 182-187.
  • 20. Perumal V, Repally A, Dasari A, et al (2016): Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes. Prep Biochem Biotech, 46, 686-694.
  • 21. Savadago A, Ouattara CAT, Bassole IHN (2004): Antimicrobial activities of lactic acid bacteria strains isolated from Burkinofaso fermented milk. Pak J Nutr, 3, 174-179.
  • 22. Sfaxi IH, Ghaish SE, Ahmadova A, et al (2011): Antimicrobial activity and safety of use of Enterococcus faecium PC4.1 isolated from Mongol yogurt. Food Control, 22, 2020-2027.
  • 23. Sivakumar N, Saif R, Saif AB (2010): Partial characterization of bacteriocins produced by Lactobacillus acidophilus and Pediococcus acidilactici. Braz Arch Biol Techn, 53, 1177-1184.
  • 24. Sparo MD, Castro MS, Andino PJ, et al (2006): Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis. J Appl Microbiol, 100, 123-134.
  • 25. Theppangna W, Murase T, Tokumaru N, et al (2007): Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci, 69, 1235-1239.
  • 26. Toit MD, Franz CMAP, Dicks LMT, et al (2000): Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces. J Appl Microbiol, 88, 482-494.
  • 27. Tulini FL., Gomes BC, Martinis ECP (2011): Partial purifcation and characterization of a bacteriocin produced by Enterococcus faecium 130 isolated from mozzarella cheese. Ciênc Tecnol Aliment, 31, 155-159.
  • 28. Vimont A, Fernandez B, Hammami R, et al (2017): Bacteriocin-producing Enterococcus faecium lcw44: a high potential probiotic candidate from raw camel milk. Front Microbiol, 8, 1-8.
  • 29. Vijayakumar PP, Muriana PM (2017): Inhibition of Listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. Foods, 6, 1-13.
  • 30. Yildirim Z, İlk Y, Yildirim M, et al (2014): Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk. Turk J Biol, 38, 412-419.

Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria

Year 2019, , 373 - 378, 09.09.2019

Zerrin Erginkaya , Hatice Uludağ Emel Ünal Turhan

https://doi.org/10.33988/auvfd.543426

Abstract

The purpose of the
present research was to obtain enterocins from bacteriocinogenic enterococci (Enterococcus faecalis and Enterococcus faecium) in clinical and
food sources, and to determine antibacterial activity of these enterocins
against pathogenic bacteria including Escherichia
coli, Staphylococcus aureus, Bacillus cereus and Salmonella Enteritidis. Enterocins were partially purified with
ammonium sulfate precipitation from E.
faecium and E. faecalis. After
purification, the antimicrobial activity of enterocin was tested on Mueller
Hinton Agar by disk diffusion assay. The 13, 8, 4 and 1 of 20 bacteriocins
obtained by Enterococcus strain
showed antimicrobial effect against S. Enteritidis,
B. cereus, E. coli and S. aureus,
respectively. One of food origin Enterococcus
(E. faecium) exhibited the
antimicrobial effect on all of the pathogen microorganisms used in our study.
Enterocins from food and clinical isolates were very effective against Salmonella Enteritidis. The most active enterocins were
produced by enterococci isolates from Hatay cow cheese due to their
antibacterial spectrum on pathogenic bacteria used in this study. This study
concluded the importance of investigating clinical enterococci besides
foodborne enterococci to benefit from antibacterial properties.

Keywords

Antibacterial bacteriocin enterocin enterococci

References

  • 1. Achemchem F, Bueno MM, Abrini J et al (2005): Enterococcus faecium F58, a bacteriocinogenic strain naturally occurring in Jben, a soft, farm house goat’s cheese made in Morocco. J Appl Microbiol, 99, 141-15.
  • 2. Anandani JH, Khan ZH (2014): Isolation, partial purification and biochemical characterization of enterocin producing enterococci. Indian J Appl Res, 4, 18-20.
  • 3. Aroujo TF, Ferreira LLF (2013): The genus Enterococcus as probiotic: Safety concerns. Braz Arch Biol Techn, 56, 457-466.
  • 4. Belicová A, Mikulášová M, Krajčovič J et al (2011): Antibacterial activity and enterocin genes in enterococci isolated from Bryndza cheese. J Food Nutr Res, 50, 21–32.
  • 5. Belguesmia Y, Choiset Y, Pr´evost H et al (2010): Partial purification and characterization of the mode of action of enterocin s37:a bacteriocin produced by Enterococcus faecalis S37 isolated from poultry feces. J Environ Pub Health, 986460, 1-8.
  • 6. Cisneros YMA, Espuñes TRS, Wacher C et al (2011): Enterocins: Bacteriocins with Applications in the Food Industry. 1330-1341. In: A. Méndez-Vilas (Ed), Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, Microbiology Series (No 3). Formatex, Badajoz, Spain.
  • 7. De Vuyst L, Morenoa MRF Revets H (2003): Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int J Food Microbiol, 84, 299-318.
  • 8. Franz CMAP, Toit MD, Holy A et al (1997): Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. J Basic Microbiol, 37, 187-196.
  • 9. Ghrairi T, Free J, Berjeaud JM et al (2008): Purification and characterization of bacteriocin produced by Enterococcus faecium from Tunisian Rigouta Cheese. Food Control, 19, 162-169.
  • 10. Gómez NC, Abriouel H, Grande MJ et al (2012): Effect of enterocin AS-8 in combination with biocides on planktonic and sessile Listeria monocytogenes. Food Microbiol, 30, 51-58.
  • 11. Isleroglu H, Yildirim Z, Tokatli M (2012): Partial characterization of enterocin KP produced by Enterococcus faecalis KP, a cheese isolate. Int J Dairy Techn, 65, 90-97.
  • 12. Javed A, Masud T, Ain Q et al (2011): Enterocins of Enterococcus faecium, emerging natural food preservatives. Ann Microbiol, 61, 699-708.
  • 13. Javed I, Ali MI, Ahmed B, et al (2011): Optimization and partial purification of bacteriocins from Enterococcus spp. indigenous to Pakistan. Food Biotechn, 25, 130-139.
  • 14. Khalkhali S, Mojgani N (2017): Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk. Iranian J Microbiol, 9, 224-233.
  • 15. Marekova M, Laukova A, De Vuyst L, et al (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J Appl Microbiol, 94, 523-530.
  • 16. Moreno MRF, Callewaert R, Devreese B, et al (2003): Isolation and biochemical characterization of enterocins produced by enterococci from different sources. J Applied Microbiol, 94, 214-229.
  • 17. Nascimento MS, Moreno I, Kuaye AY (2010): Antimicrobial activity of Enterococcus faecium fair-E 198 against Gram-Positive pathogens. Braz J Microbiol, 41, 74-81.
  • 18. Ogaki MB, Rocha KR, Terra MR, et al (2016): Screening of the enterocin-encoding genes and antimicrobial activity in Enterococcus species. J Microbiol Biotechn., 26, 1026-1034.
  • 19. Özdemir GB, Oryaşın E, Bıyık HH, et al (2011): Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian J Microbiol, 51, 182-187.
  • 20. Perumal V, Repally A, Dasari A, et al (2016): Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes. Prep Biochem Biotech, 46, 686-694.
  • 21. Savadago A, Ouattara CAT, Bassole IHN (2004): Antimicrobial activities of lactic acid bacteria strains isolated from Burkinofaso fermented milk. Pak J Nutr, 3, 174-179.
  • 22. Sfaxi IH, Ghaish SE, Ahmadova A, et al (2011): Antimicrobial activity and safety of use of Enterococcus faecium PC4.1 isolated from Mongol yogurt. Food Control, 22, 2020-2027.
  • 23. Sivakumar N, Saif R, Saif AB (2010): Partial characterization of bacteriocins produced by Lactobacillus acidophilus and Pediococcus acidilactici. Braz Arch Biol Techn, 53, 1177-1184.
  • 24. Sparo MD, Castro MS, Andino PJ, et al (2006): Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis. J Appl Microbiol, 100, 123-134.
  • 25. Theppangna W, Murase T, Tokumaru N, et al (2007): Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci, 69, 1235-1239.
  • 26. Toit MD, Franz CMAP, Dicks LMT, et al (2000): Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces. J Appl Microbiol, 88, 482-494.
  • 27. Tulini FL., Gomes BC, Martinis ECP (2011): Partial purifcation and characterization of a bacteriocin produced by Enterococcus faecium 130 isolated from mozzarella cheese. Ciênc Tecnol Aliment, 31, 155-159.
  • 28. Vimont A, Fernandez B, Hammami R, et al (2017): Bacteriocin-producing Enterococcus faecium lcw44: a high potential probiotic candidate from raw camel milk. Front Microbiol, 8, 1-8.
  • 29. Vijayakumar PP, Muriana PM (2017): Inhibition of Listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. Foods, 6, 1-13.
  • 30. Yildirim Z, İlk Y, Yildirim M, et al (2014): Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk. Turk J Biol, 38, 412-419.
There are 30 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Articles
Authors

Zerrin Erginkaya 0000-0001-6208-2927

Hatice Uludağ 0000-0003-2085-8656

Emel Ünal Turhan 0000-0002-0284-574X

Publication Date September 9, 2019
Published in Issue Year 2019

Cite

APA Erginkaya, Z., Uludağ, H., & Ünal Turhan, E. (2019). Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(4), 373-378. https://doi.org/10.33988/auvfd.543426
AMA Erginkaya Z, Uludağ H, Ünal Turhan E. Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Univ Vet Fak Derg. September 2019;66(4):373-378. doi:10.33988/auvfd.543426
Chicago Erginkaya, Zerrin, Hatice Uludağ, and Emel Ünal Turhan. “Antibacterial Activity of Partially Purified Enterocins from Foodborne and Clinical Enterococci Against Some Pathogenic Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66, no. 4 (September 2019): 373-78. https://doi.org/10.33988/auvfd.543426.
EndNote Erginkaya Z, Uludağ H, Ünal Turhan E (September 1, 2019) Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 4 373–378.
IEEE Z. Erginkaya, H. Uludağ, and E. Ünal Turhan, “Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria”, Ankara Univ Vet Fak Derg, vol. 66, no. 4, pp. 373–378, 2019, doi: 10.33988/auvfd.543426.
ISNAD Erginkaya, Zerrin et al. “Antibacterial Activity of Partially Purified Enterocins from Foodborne and Clinical Enterococci Against Some Pathogenic Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66/4 (September 2019), 373-378. https://doi.org/10.33988/auvfd.543426.
JAMA Erginkaya Z, Uludağ H, Ünal Turhan E. Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Univ Vet Fak Derg. 2019;66:373–378.
MLA Erginkaya, Zerrin et al. “Antibacterial Activity of Partially Purified Enterocins from Foodborne and Clinical Enterococci Against Some Pathogenic Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 66, no. 4, 2019, pp. 373-8, doi:10.33988/auvfd.543426.
Vancouver Erginkaya Z, Uludağ H, Ünal Turhan E. Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Univ Vet Fak Derg. 2019;66(4):373-8.