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Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits

Year 2011, , 11 - 16, 01.03.2011

Basaran Karademır

https://doi.org/10.1501/Vetfak_0000002442

Abstract

The oral zinc (Zn) applications may be required in the case of Zn losses in the body. Lots of Zn forms and compounds are used for this aim. Sulfate form of Zn is used in a widespread manner with in distillated water or other diluent solutions. The purpose of this study is to reveal the effect of some acidifier agents as diluent solutions (therapeutic dose of ascorbic acid in distillated water and pure vinegar of grapes) versus distillated water, well known and commonly used. Thirty New Zealand rabbits in three equal groups were used in this study. Zinc sulfate solutions with different diluents as above were given to groups orally. Before and after (2,5 hours later) oral applications, blood samples were collected via cardiac puncture under general diethyl ether anesthesia. Atomic absorption spectrometer equipped with flame system was employed. Significant differences (p≤0.001) were found between first and last serum Zn levels in all groups. There were also significant differences among last samples of groups (minimum p<0.05). Throughout the experiment time significant differences were found between control group and acidifier groups (ascorbic acid and vinegar of grapes) (p<0.05), but not between two acidifier groups (p>0.05). Significant correlation was detected between pH and serum Zn levels of oral solutions (r = -0.838, p<0.001). It was also observed that the oral solutions pH of ZnSO4influenced the serum Zn level significantly (r2: 70.1%, p<0.001). Consequently, the acidity of oral ZnSO4 fortification solution affected the serum Zn level. While the pH level of oral ZnSO4solutions was decreasing, serum Zn level increased. The therapeutic dose of ascorbic acid in distillated water was more effective than pure vinegar of grapes

Keywords

Ascorbic Acid serum Zn level rabbit vinegar of grapes Zn fortification

References

  • Ahmed A, Anjum FM, Ur Rehman S, Randhawa MA, Farooq U (2008): Bioavailability of calcium, iron and zinc fortified whole wheat flour chapatti. Plant Foods Hum nutr, 63, 7-13.
  • Andermann G, Dietz M (1982): The bioavailability and pharmacokinetics of three zinc salts: zinc pantothenate, zinc sulfate and zinc orotate. Eur J Drug Metab Pharmacokinet, 7, 233-239.
  • Atakişi O, Özcan A, Atakişi E, Öğün M, Kaya N (2007): Gebelik süresince çinko verilen tuj ırkı koyunlarda serum lösin aminopeptidaz aktivitesinin belirlenmesi. Kafkas Univ Vet Fak Derg, 13, 17-20.
  • Dunicz-Sokolowska A, Graczyk A, Radomska K, Dlugaszek E, Surkont G (2006): Content of bioelements and toxic metals in a Polish potulation determined by hair analysis. Part 2. Young persons aged 10-20 years. Magnes Res, 19, 167-179.
  • Ergün G, Saktaş S (2009): ANOVA modellerinde kareler toplamı yöntemlerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 15, 481-484.
  • Grace ND, Lee J (1992): Influence of high zinc intakes, season and staple site on the elemental composition of wool and fleece quality in grazing sheep. New Zealand J Agri Res, 35, 367-377.
  • Hampton L, Miller WJ, Neathery MW, Kincaid RL, Blackmon DM, Gentry RP (1976): Absorption of Zinc from Small and Large Intestine of Calves. J Dairy Sci, 59, 1963-1966.
  • Handerson LM, Brewer GJ, Dressman JB, Swidan SZ, Duross DJ, Adair CH, Barnett JL, Berardi RR (1995): Effect of intragastric pH on the absorption of oral zinc acetate and zinc oxide in young healthy volunteers. JPEN J Parenter Enteral Nutr, 19, 393-397.
  • Harvey LJ, Dainty JR, Hollands WJ, Bull VJ, Hoogewerff JA, Foxall RJ, Mcanena L, Strain JJ, Fairweather-Tait SJ (2007): Effect of high-dose iron supplements on fractional zinc absorption and status in pregnant women. Am J Clin Nutr, 85, 131-136.
  • Hotz C, Dehaene J, Woodhouse LR, Villalpando S, Rivera JA, King JC (2005): Zinc absorption from zinc oxide, zinc sulfate, zinc oxide + EDTA, or sodium-zinc EDTA does not differ when added as fortificants to maize tortillas. J Nutr, 135, 1102-1105.
  • Hotz C, Lowe NM, Araya M, Brown KH (2003): Assesment of the trace element status of individuals and populations: the example of zinc and copper. Am Soc Nutri Sci, 133, 1563S-1568S.
  • Karademir B (2007): Comparisons of some sample preparation methods for blood-serum copper and zinc at atomic absorption spectrometer (Article in Tukish). Kafkas Univ Vet Fak Derg, 13, 61-66.
  • Karademir B (2007): Effect of stress induced by vaccination on blood plasma copper, zinc, potassium and magnesium (Article in Tukish). Kafkas Univ Vet Fak Derg, 13, 49-54.
  • Karademir B (2009): The effects of oral levothyroxine sodium application on serum copper concentration in rabbits. Kafkas Univ Vet Fak Derg, 15, 937-942.
  • Karademir B, Eseceli H, Kart A (2010): The effect of oral levothyroxine sodium on serum Zn, Fe, Ca and Mg levels during acute copper sulfate toxication in rabbits. J Anim Vet Adv, 9, 240-247.
  • Karademir B, Karademir G, Tarhane S, Çiftci Ü, Koç E, Ersan Y, Bozukluhan K (2009): The effect of oral ampicillin aplicaitons on liver mineral status. J Anim Vet Adv, 8, 1846-1850.
  • Keyzer JJ, Oosting E, Wolthers BG, Muskiet EAJ (1983): Zinc absorption after oral administration of zinc sulfate. Pharm Weekbl Sci, 5, 252.
  • Leblondel G, Bouil AL, Allain P (1992): Influence of thyroparathyroidectomy and thyroxine replacement on Cu and Zn cellular distribution and on the metallothonein level and induction in rats. Biol Trace Elem Res, 32, 281- 288.
  • Liu P, Yao YN, Wu SD, Dong HJ, Feng GC, Yuan XY (2005): The efficacy of deferiprone on tissues aluminum removal and copper, zinc, manganese level in rabbits. J Inorg Biochem, 99, 1733-1737.
  • McDowell LR (1992): Minerals in Animal and Human Nutrition. Academic PresS Inc, London.
  • Mocchegian E, Muzzioil M (2000): Therapeutic application of zinc in human immunodeficiency virus against opportunistic infections. J Nutr, 130, 1424S- 1431S.
  • O’Brien OK, Zavaleta N, Caulfield LE, Wen J, Abrams SA (2000): Prenatal iron supplements impair zinc absorption in pregnant Peruvian women. J Nutr, 130, 2251-2255.
  • O’Dell BL (1989): Mineral interactions relevant to nutrient requirements. J Nutr, 119 (12 Suppl), 1832-1838.
  • Ommaty R (2005): Modern İlac Rehberi. Matsa Basımevi, Ankara.
  • Özpinar H, Abas I, Bilal T, Demirel G (2001): Investigation of excretion and absorption of different zinc salts in puppies. Lab Anim, 35, 282-287.
  • Phillips RW (1988): Water soluble vitamins. 698-702. In: NH Booth and LE McDonald (Ed), Veterinary Pharmacology and Therapeutics. 6th edition. Iowa State University Pres. Ames, Iowa, USA.
  • Riedel-de Haen (2005): Laboratory Chemicals and Analytical Reagent. Sigma-Aldrich, Germany.
  • Romaña DL, Lonnerdal B, Brown KH (2003): Absorption of zinc from wheat products fortified with iron and either zinc sulfate or zinc oxide. Am J Clin Nutr, 78, 279-283.
  • Sandstead HH, Prasad AS, Penland JG, Beck FW, Kaplan J, Egger NG, Alcock NW, Carroll RM, Ramanujam VM, Dayal HH, Rocco CD, Plotkin RA, Zavaleta AN (2008): Zinc deficiency in Mexican American children: influence of zinc and other micronutrients on T cells, cytokines, and antiinflammatory plasma proteins. Am J Clin Nutr, 88, 1067-1073.
  • Şanlı Y, Kaya S (1991): Veteriner Farmakoloji ve İlaçla Sağaltım Seçenekleri. Feryal Mat. San. Tic. Ltd.Şti, Ankara.
  • Şanlı Y, Kaya S (1993): Veteriner İlaç Rehberi ve Uygulamalı Bilgiler El Kitabı. Medisan, Ankara.
  • Shay NF, Mangian HF (2000): Neurobiology of zinc- influenced eating behavior. J Nutr, 130, 1493S-1499S.
  • SPSS (1999): SPSS Reference Manual (Release 10.0.1) for Windows. SPSS Inc, USA.
  • Swick RA, Cheeke PR, Patton NM, Buhler DR (1982): Absorption and excretion of pyrrolizidine (Senecio) alkaloids and their effects on mineral metabolism in rabbits. J Anim Sci, 55, 1417-1424.
  • Tran C, Miller LV, Krebs NF, Lei S, Hambidge KM (2004): Zinc absorption as a function of the dose of zinc sulfate in aqueous solution. Am J Clin Nutr, 80, 1570- 1573.
  • Turnbull AJ, Wood RJ, Russell RM (1992): Hypochlorhydria does not inhibit zinc absorption in the rat. Nutr Res, 12, 999-1008.
  • Yamaguchi M, Yoshiharu T, Okada S (1980): Effect of zinc on acidity of gastric secretion in rats. Toxicol Appl Pharmacol, 54, 526-530.
  • Yousef MI, El Hendy HA, El-Demerdash FM, Elagamy EI (2002): Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicol, 175, 223-234.

Tavşanlarda farklı pH’da (askorbik asit, üzüm sirkesi ve saf suya) oral çinko sülfat uygulamalarınınserum çinko düzeyleri üzerine etkileri

Year 2011, , 11 - 16, 01.03.2011

Basaran Karademır

https://doi.org/10.1501/Vetfak_0000002442

Abstract

Vücutta çinko (Zn) kaybının olması durumunda oral Zn uygulamalarına gerek duyulabilir. Bu amaçla çok sayıda Zn formu veya bileşiği kullanılmaktadır. Zn’nin sülfat formu geniş çapta safsu veya diğer sulandırma solüsyonları ile birlikte yaygın bir şekilde kullanılmaktadır. Bu çalışmanın amacı, sulandırma solüsyonları halinde bazı asitleştirici ajanların (saf su içinde tedavi dozunda askorbik asit ve saf üzüm sirkesi) iyi bilinen ve yaygın bir şekilde kullanılan saf suya karşı etkisinin ortaya konmasıdır. Bu çalışmada üç eşit grup halinde 30 Yeni Zelanda ırkı tavşan kullanıldı. Yukarıda bahsedildiği gibi farklı sulandırmalar ile ZnSO4gruplara oral yolla verildi. Oral uygulama öncesi ve 2,5 saat sonrası dietil eter genel anestezisi altında kalpten kan örnekleri alındı. Alev sistemli atomik absorbsiyon spektrofotometre kullanıldı. Tüm gruplarda ilk ve son serum Zn düzeyleri arasındaki fark önemli bulundu (p≤0.001). Grupların son örneklerinin arasında da önemli farklılıklar vardı (en az p<0.05). Deney süresi boyunca kontrol grubu ve asit grupları (askorbik asit ve üzüm sirkesi) arasında önemli farklılık bulundu (p<0.05), fakat iki asit grubu arasında fark gözlenmedi (p>0.05). Oral solüsyon pH’ı ve serum Zn düzeyi arasında önemli korelasyon tespit edildi (r = -0.838, p<0.001). Aynı zamanda ZnSO4 oral solüsyon pH’ının serum Zn düzeyini önemli ölçüde etkilediği gözlendi (r2: 70.1%, p<0.001). Sonuç olarak, oral ZnSO4 takviyesinin asiditesi serum Zn düzeyini etkiledi. Oral ZnSO4 solüsyonunun pH’ı azalırken, serum Zn düzeyi arttı. Distile su içinde askorbik asitin terapotik dozu saf üzüm sirkesinden daha etkili bulundu

Keywords

Askorbik asit serum Zn düzeyi tavşan üzüm sirkesi Zn takviyesi

References

  • Ahmed A, Anjum FM, Ur Rehman S, Randhawa MA, Farooq U (2008): Bioavailability of calcium, iron and zinc fortified whole wheat flour chapatti. Plant Foods Hum nutr, 63, 7-13.
  • Andermann G, Dietz M (1982): The bioavailability and pharmacokinetics of three zinc salts: zinc pantothenate, zinc sulfate and zinc orotate. Eur J Drug Metab Pharmacokinet, 7, 233-239.
  • Atakişi O, Özcan A, Atakişi E, Öğün M, Kaya N (2007): Gebelik süresince çinko verilen tuj ırkı koyunlarda serum lösin aminopeptidaz aktivitesinin belirlenmesi. Kafkas Univ Vet Fak Derg, 13, 17-20.
  • Dunicz-Sokolowska A, Graczyk A, Radomska K, Dlugaszek E, Surkont G (2006): Content of bioelements and toxic metals in a Polish potulation determined by hair analysis. Part 2. Young persons aged 10-20 years. Magnes Res, 19, 167-179.
  • Ergün G, Saktaş S (2009): ANOVA modellerinde kareler toplamı yöntemlerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 15, 481-484.
  • Grace ND, Lee J (1992): Influence of high zinc intakes, season and staple site on the elemental composition of wool and fleece quality in grazing sheep. New Zealand J Agri Res, 35, 367-377.
  • Hampton L, Miller WJ, Neathery MW, Kincaid RL, Blackmon DM, Gentry RP (1976): Absorption of Zinc from Small and Large Intestine of Calves. J Dairy Sci, 59, 1963-1966.
  • Handerson LM, Brewer GJ, Dressman JB, Swidan SZ, Duross DJ, Adair CH, Barnett JL, Berardi RR (1995): Effect of intragastric pH on the absorption of oral zinc acetate and zinc oxide in young healthy volunteers. JPEN J Parenter Enteral Nutr, 19, 393-397.
  • Harvey LJ, Dainty JR, Hollands WJ, Bull VJ, Hoogewerff JA, Foxall RJ, Mcanena L, Strain JJ, Fairweather-Tait SJ (2007): Effect of high-dose iron supplements on fractional zinc absorption and status in pregnant women. Am J Clin Nutr, 85, 131-136.
  • Hotz C, Dehaene J, Woodhouse LR, Villalpando S, Rivera JA, King JC (2005): Zinc absorption from zinc oxide, zinc sulfate, zinc oxide + EDTA, or sodium-zinc EDTA does not differ when added as fortificants to maize tortillas. J Nutr, 135, 1102-1105.
  • Hotz C, Lowe NM, Araya M, Brown KH (2003): Assesment of the trace element status of individuals and populations: the example of zinc and copper. Am Soc Nutri Sci, 133, 1563S-1568S.
  • Karademir B (2007): Comparisons of some sample preparation methods for blood-serum copper and zinc at atomic absorption spectrometer (Article in Tukish). Kafkas Univ Vet Fak Derg, 13, 61-66.
  • Karademir B (2007): Effect of stress induced by vaccination on blood plasma copper, zinc, potassium and magnesium (Article in Tukish). Kafkas Univ Vet Fak Derg, 13, 49-54.
  • Karademir B (2009): The effects of oral levothyroxine sodium application on serum copper concentration in rabbits. Kafkas Univ Vet Fak Derg, 15, 937-942.
  • Karademir B, Eseceli H, Kart A (2010): The effect of oral levothyroxine sodium on serum Zn, Fe, Ca and Mg levels during acute copper sulfate toxication in rabbits. J Anim Vet Adv, 9, 240-247.
  • Karademir B, Karademir G, Tarhane S, Çiftci Ü, Koç E, Ersan Y, Bozukluhan K (2009): The effect of oral ampicillin aplicaitons on liver mineral status. J Anim Vet Adv, 8, 1846-1850.
  • Keyzer JJ, Oosting E, Wolthers BG, Muskiet EAJ (1983): Zinc absorption after oral administration of zinc sulfate. Pharm Weekbl Sci, 5, 252.
  • Leblondel G, Bouil AL, Allain P (1992): Influence of thyroparathyroidectomy and thyroxine replacement on Cu and Zn cellular distribution and on the metallothonein level and induction in rats. Biol Trace Elem Res, 32, 281- 288.
  • Liu P, Yao YN, Wu SD, Dong HJ, Feng GC, Yuan XY (2005): The efficacy of deferiprone on tissues aluminum removal and copper, zinc, manganese level in rabbits. J Inorg Biochem, 99, 1733-1737.
  • McDowell LR (1992): Minerals in Animal and Human Nutrition. Academic PresS Inc, London.
  • Mocchegian E, Muzzioil M (2000): Therapeutic application of zinc in human immunodeficiency virus against opportunistic infections. J Nutr, 130, 1424S- 1431S.
  • O’Brien OK, Zavaleta N, Caulfield LE, Wen J, Abrams SA (2000): Prenatal iron supplements impair zinc absorption in pregnant Peruvian women. J Nutr, 130, 2251-2255.
  • O’Dell BL (1989): Mineral interactions relevant to nutrient requirements. J Nutr, 119 (12 Suppl), 1832-1838.
  • Ommaty R (2005): Modern İlac Rehberi. Matsa Basımevi, Ankara.
  • Özpinar H, Abas I, Bilal T, Demirel G (2001): Investigation of excretion and absorption of different zinc salts in puppies. Lab Anim, 35, 282-287.
  • Phillips RW (1988): Water soluble vitamins. 698-702. In: NH Booth and LE McDonald (Ed), Veterinary Pharmacology and Therapeutics. 6th edition. Iowa State University Pres. Ames, Iowa, USA.
  • Riedel-de Haen (2005): Laboratory Chemicals and Analytical Reagent. Sigma-Aldrich, Germany.
  • Romaña DL, Lonnerdal B, Brown KH (2003): Absorption of zinc from wheat products fortified with iron and either zinc sulfate or zinc oxide. Am J Clin Nutr, 78, 279-283.
  • Sandstead HH, Prasad AS, Penland JG, Beck FW, Kaplan J, Egger NG, Alcock NW, Carroll RM, Ramanujam VM, Dayal HH, Rocco CD, Plotkin RA, Zavaleta AN (2008): Zinc deficiency in Mexican American children: influence of zinc and other micronutrients on T cells, cytokines, and antiinflammatory plasma proteins. Am J Clin Nutr, 88, 1067-1073.
  • Şanlı Y, Kaya S (1991): Veteriner Farmakoloji ve İlaçla Sağaltım Seçenekleri. Feryal Mat. San. Tic. Ltd.Şti, Ankara.
  • Şanlı Y, Kaya S (1993): Veteriner İlaç Rehberi ve Uygulamalı Bilgiler El Kitabı. Medisan, Ankara.
  • Shay NF, Mangian HF (2000): Neurobiology of zinc- influenced eating behavior. J Nutr, 130, 1493S-1499S.
  • SPSS (1999): SPSS Reference Manual (Release 10.0.1) for Windows. SPSS Inc, USA.
  • Swick RA, Cheeke PR, Patton NM, Buhler DR (1982): Absorption and excretion of pyrrolizidine (Senecio) alkaloids and their effects on mineral metabolism in rabbits. J Anim Sci, 55, 1417-1424.
  • Tran C, Miller LV, Krebs NF, Lei S, Hambidge KM (2004): Zinc absorption as a function of the dose of zinc sulfate in aqueous solution. Am J Clin Nutr, 80, 1570- 1573.
  • Turnbull AJ, Wood RJ, Russell RM (1992): Hypochlorhydria does not inhibit zinc absorption in the rat. Nutr Res, 12, 999-1008.
  • Yamaguchi M, Yoshiharu T, Okada S (1980): Effect of zinc on acidity of gastric secretion in rats. Toxicol Appl Pharmacol, 54, 526-530.
  • Yousef MI, El Hendy HA, El-Demerdash FM, Elagamy EI (2002): Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicol, 175, 223-234.
There are 38 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA45KU55NU
Journal Section Research Article
Authors

Basaran Karademır

Publication Date March 1, 2011
Published in Issue Year 2011

Cite

APA Karademır, B. (2011). Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 58(1), 11-16. https://doi.org/10.1501/Vetfak_0000002442
AMA Karademır B. Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits. Ankara Univ Vet Fak Derg. March 2011;58(1):11-16. doi:10.1501/Vetfak_0000002442
Chicago Karademır, Basaran. “Effects of Oral Zinc Sulfate Applications at Different PH (ascorbic Acid, Vinegar of Grapes and Distillated Water) on Serum Zinc Levels in Rabbits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58, no. 1 (March 2011): 11-16. https://doi.org/10.1501/Vetfak_0000002442.
EndNote Karademır B (March 1, 2011) Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58 1 11–16.
IEEE B. Karademır, “Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits”, Ankara Univ Vet Fak Derg, vol. 58, no. 1, pp. 11–16, 2011, doi: 10.1501/Vetfak_0000002442.
ISNAD Karademır, Basaran. “Effects of Oral Zinc Sulfate Applications at Different PH (ascorbic Acid, Vinegar of Grapes and Distillated Water) on Serum Zinc Levels in Rabbits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58/1 (March 2011), 11-16. https://doi.org/10.1501/Vetfak_0000002442.
JAMA Karademır B. Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits. Ankara Univ Vet Fak Derg. 2011;58:11–16.
MLA Karademır, Basaran. “Effects of Oral Zinc Sulfate Applications at Different PH (ascorbic Acid, Vinegar of Grapes and Distillated Water) on Serum Zinc Levels in Rabbits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 58, no. 1, 2011, pp. 11-16, doi:10.1501/Vetfak_0000002442.
Vancouver Karademır B. Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits. Ankara Univ Vet Fak Derg. 2011;58(1):11-6.