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Studies on vaccine development for ovine caseous lymphadenitis

Year 2010, , 161 - 165, 01.09.2010

Müjgan İzgür Mehmet Akan Ziya İlhan Nahit Yazıcıoğlu

https://doi.org/10.1501/Vetfak_0000002371
Cited By: 1

Abstract

Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years. In this study, it was aimed to identify major antigens of Corynebacterium pseudotuberculosis strains isolated in Turkey and to evaluate the efficiency of the vaccine prepared from local strains of C. pseudotuberculosis against bacterial challenge in lambs. C. pseudotuberculosis strains were isolated from six different flocks in Turkey and were classified according to their similarities by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE). SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses. Lambs were vaccinated with a bacterin + toxoid + Freund’s complete adjuvant(FCA) vaccine, and the vaccine efficiency against bacteriological challenge was evaluated. When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x108 cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep

Keywords

Caseous lymphadenitis Corynebacterium pseudotuberculosis sheep vaccine

References

  • Batey RG (1986): Frequency and consequence of caseous lymphadenitis in sheep and lambs slaughtered and a western Australian abattoir. Am J Vet Res, 47, 482-485.
  • Braithwaite CE, Smith EE, Songer JG, Reine AH (1993): Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis. Vet Microbiol, 38, 59-70.
  • Brogden KA, Chedid L, Cutlip RC, Lehmkuhl HD, Sacks J (1990): Effect of muramyl dipeptide on immnunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. Am J Vet Res, 51, 200-202.
  • Brogden KA, Cutlip RC, Lehmkuhl HD (1984): Comparison of protection induced in lambs by Corynebacterium pseudotuberculosis whole cell and cell wall vaccines. Am J Vet Res, 45, 2393-2395.
  • Brown CC, Olander HJ (1987): Caseous lymphadenitis of goats and sheep: A review. Vet Bull, 57, 1-11.
  • Brown CC, Olander HJ, Biberstein EL, Morse SM (1986): Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis. Am J Vet Res, 47, 1116-1119.
  • Burrell DH (1980): A haemolysis inhibition test for detection of antibody to Corynebacterium ovis exotoxin. Res Vet Sci, 28, 190-194.
  • Cummins CS (1962): Chemical composition and antigenic structure of cell walls of Corynebacterium, Mycobacterium, Actinomyces and Athrobacter. J Gen Microbiol, 28, 3505-3510.
  • Eggleton DG, Doidge CV, Middleton HD, Minty DW (1991): Immunisation against ovine caseous lymphadenitis: Efficacy of monocomponent Corynebacterium pseudotuberculosis toxoid vaccine and combined clostridial- corynebacterial vaccines. Aust Vet J, 68, 320-321.
  • Ellis JA, Hawk DA, Mills KW, Pratt DL (1991): Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate. Vet Immunol Immunopathol, 28, 303-316.
  • Izgür M, Akan M, Ilhan Z (1999): Koyunlarda kazeöz lenfadenitis olgularından izole edilen etkenler. Ankara Univ Vet Fak Derg, 46, 43-50.
  • Keskintepe H (1976): Stabilization of Corynebacterium ovis antigens for serum agglutination test. Fırat Üniv Vet Fak Derg, 3, 84-93.
  • LeaMaster BR, Shen DT, Gorham JR, Leathers VW, Wells HD (1987): Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunological protection of sheep against development of caseous lymphadenitis. Am J Vet Res, 48, 869-872.
  • Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Muckle CA, Gyles CL (1982): Characterization of strains of Corynebacterium pseudotuberculosis. Can J Comp Med, 46, 206-208.
  • Muckle CA, Menzies PI, Hwang TY, van Wesenbeeck M (1992): Analysis of the immunodominant antigens of Corynebacterium pseudotuberculosis. Vet Microbiol, 30, 47-58.
  • Paton MW, Walker SB, Rose SB, Watt GF (2003): Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks. Aust Vet J 81, 91- 95.
  • Piontkowski MD, Shivvers DW(1998): Evaluation of a commercially available vaccine against Corynebacterium pseudotuberculosis for use in sheep. J Am Vet Med Assoc, 212, 1765-1768.
  • Renshaw HW, Graff VP, Gates NL (1979): Visceral caseous lymphadenitis in thin ewe syndrome: Isolation of Corynebacterium, Staphylococcus, and Moraxella spp. from internal abscesses in emaciated eyes. Am J Vet Res, 40, 1110-1114.
  • Schreuder BEC, ter Laak EA, Dercksen DP (1994): Eradication of caseous lymphadenitis in sheep with the help of a newly developed ELISA technique. Vet Rec, 135, 174-176.
  • Simon MC, Alonso JL, Muzquiz JL, Girones O, Ortega C, Garcia J (1992) : Vaccination contre la maladie des abces du mouton etude de ıeficacite des quatre vaccines testes sur souris. Vet Med, 168, 35-41.
  • Songer JG, Libby SJ, Iandolo JJ, Cuevas WA (1988): Biochemical and genetic characterization of Corynebacterium pseudotuberculosis. Am J Vet Res, 49, 223-226.
  • Sutherland SS, Speijers EJ, Andres B (1989): Comparison of the exotoxins of four strains of Corynebacterium pseudotuberculosis. Res Vet Sci, 47, 190-194.
  • Thrusfield M (1986): Veterinary Epidemiology. Butterworth Co., London, pp. 175-186.
  • Vauterin L, Awings J, Kerters K (1993): Protein electrophoresis and classification. 251-272. In: Goodfellow M, O’Donnell AG (Eds), Handbook of New Bacterial Systematics. Academic Press, London.

Koyun kazeöz lenfadenitisine karşı aşı geliştirme çalışmaları

Year 2010, , 161 - 165, 01.09.2010

Müjgan İzgür Mehmet Akan Ziya İlhan Nahit Yazıcıoğlu

https://doi.org/10.1501/Vetfak_0000002371
Cited By: 1

Abstract

Koyun ve keçilerin önemli bir infeksiyonu olan kazeöz lenfadenitis (KLA), son zamanlarda Türkiye’de önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, Türkiye’den izole edilen farklı Corynebacterium pseudotuberculosis suşlarının major antijenlerinin belirlenmesi ve lokal suşlardan hazırlanan aşıların, toklularda eprüvasyona karşı koruyuculuğunun ortaya konulması amaçlandı. Altı farklı sürüden izole edilen C. pseudotuberculosis suşları, sodyum dodesil sulfat-poliakrilamid jel elektroforezis (SDS-PAGE) ile analiz edildiğinde, molekül ağırlıkları 15-194 kDa arasında değişen 21 bant tespit edildi. Aşı denemeleri toklularda gerçekleştirildi ve hayvanlar, C. pseudotuberculosis’in bakterin + toksoid + Freund’un komple adjuvantı (FKA)’nı içeren aşı ile aşılandı. Sonuç olarak, lokal C. pseudotuberculosis (Pl 18) suşunun, 1.5x108 cfu/ml bakterin + toxoid + 1/2 FKA’tı şeklinde formüle edilip, aşı olarak kullanılmasının, koyunları KLA’e karşı korumada başarılı olduğu tespit edildi

Keywords

Aşı Corynebacterium pseudotuberculosis kazeöz lenfadenitis koyun

References

  • Batey RG (1986): Frequency and consequence of caseous lymphadenitis in sheep and lambs slaughtered and a western Australian abattoir. Am J Vet Res, 47, 482-485.
  • Braithwaite CE, Smith EE, Songer JG, Reine AH (1993): Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis. Vet Microbiol, 38, 59-70.
  • Brogden KA, Chedid L, Cutlip RC, Lehmkuhl HD, Sacks J (1990): Effect of muramyl dipeptide on immnunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. Am J Vet Res, 51, 200-202.
  • Brogden KA, Cutlip RC, Lehmkuhl HD (1984): Comparison of protection induced in lambs by Corynebacterium pseudotuberculosis whole cell and cell wall vaccines. Am J Vet Res, 45, 2393-2395.
  • Brown CC, Olander HJ (1987): Caseous lymphadenitis of goats and sheep: A review. Vet Bull, 57, 1-11.
  • Brown CC, Olander HJ, Biberstein EL, Morse SM (1986): Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis. Am J Vet Res, 47, 1116-1119.
  • Burrell DH (1980): A haemolysis inhibition test for detection of antibody to Corynebacterium ovis exotoxin. Res Vet Sci, 28, 190-194.
  • Cummins CS (1962): Chemical composition and antigenic structure of cell walls of Corynebacterium, Mycobacterium, Actinomyces and Athrobacter. J Gen Microbiol, 28, 3505-3510.
  • Eggleton DG, Doidge CV, Middleton HD, Minty DW (1991): Immunisation against ovine caseous lymphadenitis: Efficacy of monocomponent Corynebacterium pseudotuberculosis toxoid vaccine and combined clostridial- corynebacterial vaccines. Aust Vet J, 68, 320-321.
  • Ellis JA, Hawk DA, Mills KW, Pratt DL (1991): Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate. Vet Immunol Immunopathol, 28, 303-316.
  • Izgür M, Akan M, Ilhan Z (1999): Koyunlarda kazeöz lenfadenitis olgularından izole edilen etkenler. Ankara Univ Vet Fak Derg, 46, 43-50.
  • Keskintepe H (1976): Stabilization of Corynebacterium ovis antigens for serum agglutination test. Fırat Üniv Vet Fak Derg, 3, 84-93.
  • LeaMaster BR, Shen DT, Gorham JR, Leathers VW, Wells HD (1987): Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunological protection of sheep against development of caseous lymphadenitis. Am J Vet Res, 48, 869-872.
  • Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Muckle CA, Gyles CL (1982): Characterization of strains of Corynebacterium pseudotuberculosis. Can J Comp Med, 46, 206-208.
  • Muckle CA, Menzies PI, Hwang TY, van Wesenbeeck M (1992): Analysis of the immunodominant antigens of Corynebacterium pseudotuberculosis. Vet Microbiol, 30, 47-58.
  • Paton MW, Walker SB, Rose SB, Watt GF (2003): Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks. Aust Vet J 81, 91- 95.
  • Piontkowski MD, Shivvers DW(1998): Evaluation of a commercially available vaccine against Corynebacterium pseudotuberculosis for use in sheep. J Am Vet Med Assoc, 212, 1765-1768.
  • Renshaw HW, Graff VP, Gates NL (1979): Visceral caseous lymphadenitis in thin ewe syndrome: Isolation of Corynebacterium, Staphylococcus, and Moraxella spp. from internal abscesses in emaciated eyes. Am J Vet Res, 40, 1110-1114.
  • Schreuder BEC, ter Laak EA, Dercksen DP (1994): Eradication of caseous lymphadenitis in sheep with the help of a newly developed ELISA technique. Vet Rec, 135, 174-176.
  • Simon MC, Alonso JL, Muzquiz JL, Girones O, Ortega C, Garcia J (1992) : Vaccination contre la maladie des abces du mouton etude de ıeficacite des quatre vaccines testes sur souris. Vet Med, 168, 35-41.
  • Songer JG, Libby SJ, Iandolo JJ, Cuevas WA (1988): Biochemical and genetic characterization of Corynebacterium pseudotuberculosis. Am J Vet Res, 49, 223-226.
  • Sutherland SS, Speijers EJ, Andres B (1989): Comparison of the exotoxins of four strains of Corynebacterium pseudotuberculosis. Res Vet Sci, 47, 190-194.
  • Thrusfield M (1986): Veterinary Epidemiology. Butterworth Co., London, pp. 175-186.
  • Vauterin L, Awings J, Kerters K (1993): Protein electrophoresis and classification. 251-272. In: Goodfellow M, O’Donnell AG (Eds), Handbook of New Bacterial Systematics. Academic Press, London.
There are 25 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA48MJ95PM
Journal Section Research Article
Authors

Müjgan İzgür

Mehmet Akan

Ziya İlhan

Nahit Yazıcıoğlu

Publication Date September 1, 2010
Published in Issue Year 2010

Cite

APA İzgür, M., Akan, M., İlhan, Z., Yazıcıoğlu, N. (2010). Studies on vaccine development for ovine caseous lymphadenitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57(3), 161-165. https://doi.org/10.1501/Vetfak_0000002371
AMA İzgür M, Akan M, İlhan Z, Yazıcıoğlu N. Studies on vaccine development for ovine caseous lymphadenitis. Ankara Univ Vet Fak Derg. September 2010;57(3):161-165. doi:10.1501/Vetfak_0000002371
Chicago İzgür, Müjgan, Mehmet Akan, Ziya İlhan, and Nahit Yazıcıoğlu. “Studies on Vaccine Development for Ovine Caseous Lymphadenitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57, no. 3 (September 2010): 161-65. https://doi.org/10.1501/Vetfak_0000002371.
EndNote İzgür M, Akan M, İlhan Z, Yazıcıoğlu N (September 1, 2010) Studies on vaccine development for ovine caseous lymphadenitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57 3 161–165.
IEEE M. İzgür, M. Akan, Z. İlhan, and N. Yazıcıoğlu, “Studies on vaccine development for ovine caseous lymphadenitis”, Ankara Univ Vet Fak Derg, vol. 57, no. 3, pp. 161–165, 2010, doi: 10.1501/Vetfak_0000002371.
ISNAD İzgür, Müjgan et al. “Studies on Vaccine Development for Ovine Caseous Lymphadenitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57/3 (September 2010), 161-165. https://doi.org/10.1501/Vetfak_0000002371.
JAMA İzgür M, Akan M, İlhan Z, Yazıcıoğlu N. Studies on vaccine development for ovine caseous lymphadenitis. Ankara Univ Vet Fak Derg. 2010;57:161–165.
MLA İzgür, Müjgan et al. “Studies on Vaccine Development for Ovine Caseous Lymphadenitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 57, no. 3, 2010, pp. 161-5, doi:10.1501/Vetfak_0000002371.
Vancouver İzgür M, Akan M, İlhan Z, Yazıcıoğlu N. Studies on vaccine development for ovine caseous lymphadenitis. Ankara Univ Vet Fak Derg. 2010;57(3):161-5.

Cited By

Keçilerde Kazeöz Lenfadenitis (KLA) Hastalığına Bağlı Yüzlek Apse Sıklığının Değişimi
Hayvansal Üretim
https://doi.org/10.29185/hayuretim.363903