Letter to Editor
Localization of insulin immunpositive cells and histochemical structure of the pancreas in falcons (Falco Anaumanni)
Abstract
The present study was conducted to determine the regional distribution of insulin immunpositive cells and histochemical structure of pancreas in falcon (Falco Anaumanni). Falcon pancreas was composed of three different parts as dorsal, ventral and splenic lobes. Those lobes contained acinar cells, pancreatic ducts, alpha, beta and mixed islets. Alpha islets were determined 3-4 unit as large islets in centre of the splenic lobe, but a few as small islets in dorsal and ventral lobes. Beta islets were widespread as small clusters in all lobes which were especially 7-8 units in periphery of the splenic lobe. Furthermore, mixed islets were found abundantly as small or large islets in all lobes. In the streptavidin biotin peroxidase staining of the pancreatic lobes, insulin immunpositive cells were numerous in the centre of beta islets, on the other hand these cells were detected as only one cell or 3-8 immunpositive cell groups in both alpha islets and exocrine tissue. In conclusion, the morphology and insulin immunpositive cells of pancreas in falcons are similar to duck, whereas are different from other avian species such as chicken, goose, and quail
References
- Bancroft JD, Cook HC (1984): Manual of Histological Techniques. 42-45, 71-73, 164-165. Churchill Livingstone Medical Division Longman Group Limited, UK.
- Bertelli E, Bendayan M (1997): Intermediate endocrine- acinar pancreatic cells in duct ligation conditions. Am J Physiol Cell Physiol, 273, 1641 – 1649.
- Böck P. (1989): Romeis Microskopische Tecknik. 17. Aufl. Urban und Scwarzenberg. München.
- Böck P, Moneim M, Egerbacher M (1997): Development of pancreas. Microsc Res Techn, 37, 374 – 383.
- Cowap J (1985): The first appearence of endocrine cells in the splenic lobe of the embriyonic chick pancreas. Gen Comp Endocrin, 60, 131 – 137.
- Culling CFA, Allison RT, Barr WT (1985): Cellular Pathology Technique, pp.: 439. 4th Ed., Butterworth & Co. Ltd. London.
- Duncan DB (1955): Multiple range and multiple F test. Biometrics, 11, 1– 2.
- Egerbacher M, Böck P (1997): Morphology of the pancreatic duct system in mammals. Microsc Res Tech, 37, 407 – 17.
- Gülmez N (2003): Are glands present in goose pancreatic ducts? A light microscope study. J Pancreas, 4, 125 – 128.
- Gülmez N, Kocamış H, Aslan Ş, Nazlı M (2004): Immunohistochemical distribution of cells containing insulin, glucagon and somatostatin in the goose (Anser anser) pancreas. Turk J Vet Anim Sci, 28, 403 – 407.
- Hodges RD (1974): The Histology of the Fowl. 101 – 109. Academic Press London, New York, San Francisco.
- Ku SK, Lee JH, Lee HS (2000): An immunohistochemical study of the insulin-, glucagon- and somatostatin- immunoreactive cells in the developing pancreas of the chicken embryo. Tissue Cell, 32, 58 – 65.
- Liu JW, Evans H, Larsen P, Pan D, Xu SZ, Dong HC, Deng XB, Wan B, Gi T (1998): Gross anatomy of the pancreatic lobes and ducts in six breeds of domestic ducks and six species of wild ducks in china. Anat Histol Embryol, 27, 413 – 417.
- Lucini C, Castaldo L, Lai O (1996): An immunohistochemical study of the endocrine pancreas of ducks. Eur J Histochem, 40, 45 – 52.
- Mikami SI, Mutoh K (1977): Light- and electron- microscopic studies of the pancreatic islet cells in the chicken under normal and experimental conditions. Cell Tissue Res, 180, 453 – 465.
- Motta MP, Macchiarelli G, Nottola SA, Correr S (1997): Histology of the exocrine pancreas. Microsc Res Tech, 37, 384 – 398.
- Nagasao J, Sugiyama D, Yoshioka K, Amasaki H, An T, Yue Z, Mutoh K (2003): Morphological relationship between intercalated duct and pancreatic islet in streptozotocin and/or camostat mesilate administrations in the chicken. Anat Histol Embryol, 32, 89 – 93.
- Polak JM, Pearse AGE, Adams C, Garaud JC (1974): Immunohistochemical and ultrastructural studies on the endocrine polypeptide (APUD) cells of the avian gastrointestinal tract. CMLS, 30, 564 – 567.
- Rawdon BB (1998): Morphogenesis and differentiation of the avian endocrine pancreas, with particular reference to experimental studies on the chick embryo. Microsc Res Tech, 43, 292 – 305.
- Rawdon BB, Larsson LI (2000): Development of hormonal peptides and processing enzymes in the embryonic avian pancreas with special reference to co- localisation. Histochem Cell Biol, 114,105 –112
- Salvi E, Renda T (1986): Immunohistochemical studies on the ontogenesis of some endocrine cells in the chicken antrum and duodenum. Basic Appl Histochem, 30, 307–316.
- Simsek N, Alabay B (2008): Light and electron microscopic examinations of the pancreas in quails (Coturnix coturnix japonica). Revue Méd. Vét, 159, 198-206.
- Smith PH (1974): Pancreatic islets of the coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat Rec, 178, 567 – 585.
- Şimşek N, Özudoğru Z, Alabay B (2008): Immunohistochemical studies on the splenic lobe of the pancreas in young japanese quails (Coturnix c. japonica). Dtsch Tierärztl Wschr, 115, 189 – 193.
- Tomita T, Doull V, Pollock HG, Kimmell JR (1985): Regional distribution of pancreatic polypeptide and other hormones in chicken pancreas: reciprocal relationship between pancreatic polypeptide and glucagon. Gen Comp Endoc, 58, 303 – 310.
- Watanabe T, Chikazawa H, Yamada J (1984): Catecholamine containing pancreatic islet cells of the domestic fowl. Light, fluorescence and electron microscopy, and immunohistochemistry. Cell Tissue Res, 237, 239 – 244.
- Yukawa M, Takeuchi T, Watanabe T, Kitamura S (1999): Proportions of various endocrine cells in the pancreatic islets of wood mice (Apodemus speciosus). Anat Histol Embryol, 28, 13 – 16.
Şahinlerde (Falco Anaumanni) pankreasın histokimyasal yapısı ve insülin immunpozitif hücrelerinlokalizasyonu
Abstract
Bu çalışma, şahin (Falco Anaumanni) pankreasının histokimyasal yapısı ve insülin immunpozitif hücrelerin bölgesel dağılımını belirlemek için yapıldı. Şahin pankreası dorsal, ventral ve dalak lobu olarak 3 farklı lobdan oluşuyordu. Bu loplar asiner hücreler, akıtıcı kanallar, alfa, beta ve mix adacıkları içermekteydi. Alfa adacıkları dalak lobunun merkezinde 3-4 adet büyük adacık halinde gözlenirken, dorsal ve ventral lopta az sayıda küçük adacıklar halinde belirlendi. Beta adacıkları özellikle dalak lobunun periferinde 7-8 adet küçük adacıklar halinde bütün loplarda çok sayıda tespit edildi. Ayrıca, miks adacıklarının küçük ya da büyük adacıklar halinde pankreasın bütün loplarında çok yaygın bir şekilde bulunduğu saptandı. Pankreatik lobların streptavidin-biotin peroksidaz boyamalarında, insulin immunpozitif hücreler beta adacıkları merkezinde çok sayıdaydı diğer yandan bu hücreler hem alfa adacıklarında hem de ekzokrin dokuda yalnız tek bir hücre ya da 3-8 hücrelik gruplar halinde görüldü. Sonuç olarak şahin pankreasının morfolojik ve insulin pozitif hücreler açısından ördek pankreası ile benzer olduğu, tavuk, kaz ve bıldırcın gibi diğer kanatlı türlerinin pankreaslarından farklı olduğu tespit edilmiştir
References
- Bancroft JD, Cook HC (1984): Manual of Histological Techniques. 42-45, 71-73, 164-165. Churchill Livingstone Medical Division Longman Group Limited, UK.
- Bertelli E, Bendayan M (1997): Intermediate endocrine- acinar pancreatic cells in duct ligation conditions. Am J Physiol Cell Physiol, 273, 1641 – 1649.
- Böck P. (1989): Romeis Microskopische Tecknik. 17. Aufl. Urban und Scwarzenberg. München.
- Böck P, Moneim M, Egerbacher M (1997): Development of pancreas. Microsc Res Techn, 37, 374 – 383.
- Cowap J (1985): The first appearence of endocrine cells in the splenic lobe of the embriyonic chick pancreas. Gen Comp Endocrin, 60, 131 – 137.
- Culling CFA, Allison RT, Barr WT (1985): Cellular Pathology Technique, pp.: 439. 4th Ed., Butterworth & Co. Ltd. London.
- Duncan DB (1955): Multiple range and multiple F test. Biometrics, 11, 1– 2.
- Egerbacher M, Böck P (1997): Morphology of the pancreatic duct system in mammals. Microsc Res Tech, 37, 407 – 17.
- Gülmez N (2003): Are glands present in goose pancreatic ducts? A light microscope study. J Pancreas, 4, 125 – 128.
- Gülmez N, Kocamış H, Aslan Ş, Nazlı M (2004): Immunohistochemical distribution of cells containing insulin, glucagon and somatostatin in the goose (Anser anser) pancreas. Turk J Vet Anim Sci, 28, 403 – 407.
- Hodges RD (1974): The Histology of the Fowl. 101 – 109. Academic Press London, New York, San Francisco.
- Ku SK, Lee JH, Lee HS (2000): An immunohistochemical study of the insulin-, glucagon- and somatostatin- immunoreactive cells in the developing pancreas of the chicken embryo. Tissue Cell, 32, 58 – 65.
- Liu JW, Evans H, Larsen P, Pan D, Xu SZ, Dong HC, Deng XB, Wan B, Gi T (1998): Gross anatomy of the pancreatic lobes and ducts in six breeds of domestic ducks and six species of wild ducks in china. Anat Histol Embryol, 27, 413 – 417.
- Lucini C, Castaldo L, Lai O (1996): An immunohistochemical study of the endocrine pancreas of ducks. Eur J Histochem, 40, 45 – 52.
- Mikami SI, Mutoh K (1977): Light- and electron- microscopic studies of the pancreatic islet cells in the chicken under normal and experimental conditions. Cell Tissue Res, 180, 453 – 465.
- Motta MP, Macchiarelli G, Nottola SA, Correr S (1997): Histology of the exocrine pancreas. Microsc Res Tech, 37, 384 – 398.
- Nagasao J, Sugiyama D, Yoshioka K, Amasaki H, An T, Yue Z, Mutoh K (2003): Morphological relationship between intercalated duct and pancreatic islet in streptozotocin and/or camostat mesilate administrations in the chicken. Anat Histol Embryol, 32, 89 – 93.
- Polak JM, Pearse AGE, Adams C, Garaud JC (1974): Immunohistochemical and ultrastructural studies on the endocrine polypeptide (APUD) cells of the avian gastrointestinal tract. CMLS, 30, 564 – 567.
- Rawdon BB (1998): Morphogenesis and differentiation of the avian endocrine pancreas, with particular reference to experimental studies on the chick embryo. Microsc Res Tech, 43, 292 – 305.
- Rawdon BB, Larsson LI (2000): Development of hormonal peptides and processing enzymes in the embryonic avian pancreas with special reference to co- localisation. Histochem Cell Biol, 114,105 –112
- Salvi E, Renda T (1986): Immunohistochemical studies on the ontogenesis of some endocrine cells in the chicken antrum and duodenum. Basic Appl Histochem, 30, 307–316.
- Simsek N, Alabay B (2008): Light and electron microscopic examinations of the pancreas in quails (Coturnix coturnix japonica). Revue Méd. Vét, 159, 198-206.
- Smith PH (1974): Pancreatic islets of the coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat Rec, 178, 567 – 585.
- Şimşek N, Özudoğru Z, Alabay B (2008): Immunohistochemical studies on the splenic lobe of the pancreas in young japanese quails (Coturnix c. japonica). Dtsch Tierärztl Wschr, 115, 189 – 193.
- Tomita T, Doull V, Pollock HG, Kimmell JR (1985): Regional distribution of pancreatic polypeptide and other hormones in chicken pancreas: reciprocal relationship between pancreatic polypeptide and glucagon. Gen Comp Endoc, 58, 303 – 310.
- Watanabe T, Chikazawa H, Yamada J (1984): Catecholamine containing pancreatic islet cells of the domestic fowl. Light, fluorescence and electron microscopy, and immunohistochemistry. Cell Tissue Res, 237, 239 – 244.
- Yukawa M, Takeuchi T, Watanabe T, Kitamura S (1999): Proportions of various endocrine cells in the pancreatic islets of wood mice (Apodemus speciosus). Anat Histol Embryol, 28, 13 – 16.
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA56ZR43BB |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2009 |
| Published in Issue | Year 2009 |