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RFLP typing of infectious bursal disease virus strains detected in Turkey

Year 2006, , 129 - 136, 01.06.2006

Barış Sareyyüpoğlu Müjgan İzgür

https://doi.org/10.1501/Vetfak_0000000080

Abstract

In this study, identification of IBDVs encountered in Turkey by agar gel immunodiffusion (AGID) and reverse transcription/polymerase chain reaction (RT/PCR) test and molecular typing of IBDV strains by restriction fragment length polymorphism (RFLP) assay were aimed. For this reason, a total of 122 bursa Fabricii from 24 flocks showing the symptoms of IBD, reared in the restricts of Adana, Bolu, Bursa, Çorum and Eskişehir were investigated for the existance of viral antigens and the viral RNAs by AGID and RT/PCR tests, respectively. Molecular typing of RT/PCR positive IBDV samples by RFLP analysis using BstNI, MboI and SspI restriction enzymes was performed. After AGID tests, 102 (83.6 %) of 122 bursal samples from 24 flocks were found to have IBDV antigens. After RT/PCR tests, 47 (38.5 %) out of 122 bursa samples were found to have IBDV RNA while 75 (61.5 %) were not. Following RFLP analysis of RT/PCR positive samples, two different molecular patterns were detected. Following the restriction endonuclease analysis performed with SspI enzyme, these were found to have similar molecular patterns with the European very virulent IBDVs (vvIBDVs). As a conclusion, AGID test was found to be an appropriate test for the routine identification of IBDV strains from bursa samples and RT/PCR-RFLP test could be used for the molecular typing of IBDV virus strains in Turkey

Keywords

AGID IBDV molecular typing RT/PCR-RFLP

References

  • Ası Y, İyisan AS (1991): İnfeksiyöz bursal disease’e (IBD, Gumboro) karşı aşılı etçi piliçlerde ELISA yöntemi ile an- tikor düzeyinin saptanması ve QAGP yöntemi ile karşılaş- tırılması. Pendik Hay Hast Merk Araşt Enst Derg, 22, 107- 121.
  • Bayliss CD, Spies U, Shaw K, Peters RW, Papageorgion A, Müller H, Boursnell MEG (1990): A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol, 71, 1303-1312.
  • Brown MD, Green P, Skinner MA (1994): VP2 sequences of recent European ‘very virulent’ isolates of infectious bursal disease virus are closely related to each other but are distinct from those of ‘classical strains’. J Gen Virol, 75, 675-680.
  • Çöven F (1995) : Broyler ve Yumurtacı Tavuklarda Gum- boro (İnfectious Bursal Disease) Hastalığının İnsidensi ve Virus İzolasyonu. Uludağ Üniversitesi Veteriner Fakültesi, Doktora Tezi. s. 66.
  • Çöven F, Çarlı T (1997) : Türkiye’de broiler ve yumurtacı tavuklardan infectious bursal disease virusunun izolasyon ve identifikasyonu. Pendik Vet Mikrobiyol Derg, 28, 141- 152.
  • Ergün A (1995): Klinik ve Subklinik Gumboro Vakala- rından Virus İzolasyonu ve Serotiplendirilmesi. Selçuk Ü- niversitesi Veteriner Fakültesi, Doktora Tezi. s. 72.
  • Eterradossi N, Arnauld C, Tekaia F, Toquin D, Le Coq H, Rivallan G, Guittet M, Domenech J, van den Berg TP, Skinner MA (1999): Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate. Avian Pathol, 28, 36-46.
  • Heine H, Haritou M, Failla P, Fahey K, Azad A (1991): Sequence analysis and expression of the host-protective immunogen VP2 of a variant strain of infectious bursal disease virus which can circumvent vaccination with stan- dart type I strains. J Gen Virol, 72, 1835-1843.
  • Hoque MM, Omar AR, Chong LK, Hair-Bejo M, Aini I (2001): Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region. Avian Pathol, 30, 369-380.
  • Jackwood DJ, Nielsen CK (1997): Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction endonuclease assay. Avian Dis, 41, 137-143.
  • Jackwood DJ, Sommer SE (1997): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses. Avian Dis, 41, 627-637.
  • Jackwood DJ, Sommer SE (1998): Genetic heterogeneity in the VP2 gene of infectious bursal disease viruses detected in commercially reared chickens. Avian Dis, 42, 321-339.
  • Jackwood DJ, Sommer SE (1999): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses from outside the United States. Avian Dis, 43, 310-314.
  • Jackwood DJ, Jackwood RJ, Sommer SE (1997): Identification and comparison of point mutations associated in classic and variant infectious bursal disease viruses. Virus Res, 49, 131-137.
  • Lim BL, Cao Y, Yu T, Mo CW (1999): Adaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2. J Virol, 73, 2854-2862.
  • Lin Z, Kato A, Otaki Y, Nakamura T, Sasmaz E, Ueda S (1993): Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan. Avian Dis, 37, 315-323.
  • Liu HJ, Giambrone JJ, Dormotorio T (1994): Detection of genetic variations in serotype I isolates of infectious bursal disease virus using polymerase chain reaction and restriction endonuclease analysis. J Virol Methods, 48, 281-291.
  • Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: Calnek BW, Barnes HJ, Beard CW, McDougald LR, Saif YM (Eds.): Diseases of Poultry. 10th ed. Iowa State University Press, Ames, Iowa, USA.
  • McFerran JB, McNulty MS, McCillop E, Corner TJ, McCracken RM, Collins DS, Allan G (1980): Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys and ducks, demonstration of a second serotype. Avian Pathol, 9, 395-404.
  • Meir R, Jackwood DJ, Weisman Y (2001): Molecular typing of infectious bursal disease virus of Israeli field and vaccine strains by the reverse transcription/polymerase chain reaction/restriction fragment length polymorphism assay. Avian Dis, 45, 223-228.
  • Office International Des Epizooties (OIE) (2000): Manual of Standards for Diagnostic Tests and Vaccines. 4th Ed. OIE, Paris.
  • Pala HH, Türe O (1996): Gumboro hastalığının immunperoksidaz ve immunofloresan teknikleri ile teşhisi. Bornova Vet Kontr Araşt Enst Md Derg, 21, 161-175.
  • Rosenberger JK (1989): Infectious bursal disease. 165- 166. In: HG Purchase, LH Arp, CH Dommermuth, JE Pearson (Eds.): A Laboratory Manual for the Isolation and Identification of Avian Pathogens 3rd Ed. Kennett Square, American Association of Avian Pathologists.
  • Takase K, Uchimura T, Katsuki N, Yamamoto M (1993): Agar gel precipitin line patterns and pathogenicity of infectious bursal disease viruses. J Vet Med Sci, 55, 137-139.
  • Tham KM, Young LW, Moon CD (1995): Detection of infectious bursal disease virus by reverse-transcription- polymerase chain reaction amplification of the virus segment A gene. J Virol Meth, 53, 201-212.
  • Türe O, Saif YM, Jackwood DJ (1998): Restriction length polymorphism analysis of highly virulent strains of infectious bursal disease viruses from Holland, Turkey, and Taiwan. Avian Dis, 42, 470-479.
  • Van den Berg TP (2000): Acute infectious bursal disease in poultry: a review. Avian Pathol, 29, 175-194.
  • Van den Berg TP, Eterradossi N, Toquin D, Meulemans G (2000): Infectious Bursal Disease (Gumboro Disease). Rev Sci Tech Off İnt Epiz, 19, 527- 543.
  • Zierenberg K, Nieper H, van den Berg TP, Ezeokoli CD, Vob M, Müller H (2000): The VP2 variable region of African and German isolates of infectious bursal disease virus: comparison with very virulent, “classical” virulent, and attenuated tissue culture-adapted strains. Arch Virol, 145, 113-125.

Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi

Year 2006, , 129 - 136, 01.06.2006

Barış Sareyyüpoğlu Müjgan İzgür

https://doi.org/10.1501/Vetfak_0000000080

Abstract

Bu çalışmada, Türkiye’de İnfeksiyöz Bursal Hastalığa (IBD) neden olan virusların agar jel immunodifuzyon testi (AGID) ve reverz transkripsiyon/polimeraz zincir reaksiyonu (RT/PCR) ile saptanması ve IBDV suşlarının RFLP (restriction fragment length polymorphism) analizi ile moleküler tiplendirilmesi amaçlandı. Bu amaçla, çeşitli bölgelerdeki 24 sürüye ait toplam 122 adet bursa Fabricius materyali IBDV antijenlerinin varlığı yönünden AGID testi, IBDV viral RNA’sının varlığı yönünden ise RT/PCR testi ile değerlendirildi. RT/PCR sonucunda pozitif olduğu belirlenen virus örneklerinin moleküler tiplendirilmesi, BstNI, MboI ve SspI enzimlerinin kullanıldığı RFLP tekniği ile yapıldı. AGID testleri sonucunda incelenen 24 sürüye ait toplam 122 adet örneğin 102 (% 83.6)’si IBDV yönünden pozitif bulundu. Bursa Fabricius örneklerinden izole edilen RNA’lara uygulanan RT/PCR testi sonucunda, 24 sürüye ait toplam 122 adet materyalin 47 (% 38.5)’sinde viral genetik materyal saptandı. RT/PCR testiyle saptanan IBDV suşlarının RFLP analizi sonucunda birbirinden farklı moleküler yapı gösteren iki farklı virus suşu belirlendi. Bu suşların SspI enzimi ile incelenmesi sonucunda bunların Avrupa’da gözlenen yüksek virulensteki suşlarla benzer moleküler yapıya sahip oldukları ortaya kondu. Sonuç olarak, AGID testinin IBDV suşlarının rutin identifikasyonu için uygun bir test olduğu, RT/PCRRFLP testinin ise IBDV viruslarının tiplendirilmesinde kullanılabileceği belirlendi

Keywords

AGID IBDV moleküler tiplendirme RT/PCR-RFLP

References

  • Ası Y, İyisan AS (1991): İnfeksiyöz bursal disease’e (IBD, Gumboro) karşı aşılı etçi piliçlerde ELISA yöntemi ile an- tikor düzeyinin saptanması ve QAGP yöntemi ile karşılaş- tırılması. Pendik Hay Hast Merk Araşt Enst Derg, 22, 107- 121.
  • Bayliss CD, Spies U, Shaw K, Peters RW, Papageorgion A, Müller H, Boursnell MEG (1990): A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol, 71, 1303-1312.
  • Brown MD, Green P, Skinner MA (1994): VP2 sequences of recent European ‘very virulent’ isolates of infectious bursal disease virus are closely related to each other but are distinct from those of ‘classical strains’. J Gen Virol, 75, 675-680.
  • Çöven F (1995) : Broyler ve Yumurtacı Tavuklarda Gum- boro (İnfectious Bursal Disease) Hastalığının İnsidensi ve Virus İzolasyonu. Uludağ Üniversitesi Veteriner Fakültesi, Doktora Tezi. s. 66.
  • Çöven F, Çarlı T (1997) : Türkiye’de broiler ve yumurtacı tavuklardan infectious bursal disease virusunun izolasyon ve identifikasyonu. Pendik Vet Mikrobiyol Derg, 28, 141- 152.
  • Ergün A (1995): Klinik ve Subklinik Gumboro Vakala- rından Virus İzolasyonu ve Serotiplendirilmesi. Selçuk Ü- niversitesi Veteriner Fakültesi, Doktora Tezi. s. 72.
  • Eterradossi N, Arnauld C, Tekaia F, Toquin D, Le Coq H, Rivallan G, Guittet M, Domenech J, van den Berg TP, Skinner MA (1999): Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate. Avian Pathol, 28, 36-46.
  • Heine H, Haritou M, Failla P, Fahey K, Azad A (1991): Sequence analysis and expression of the host-protective immunogen VP2 of a variant strain of infectious bursal disease virus which can circumvent vaccination with stan- dart type I strains. J Gen Virol, 72, 1835-1843.
  • Hoque MM, Omar AR, Chong LK, Hair-Bejo M, Aini I (2001): Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region. Avian Pathol, 30, 369-380.
  • Jackwood DJ, Nielsen CK (1997): Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction endonuclease assay. Avian Dis, 41, 137-143.
  • Jackwood DJ, Sommer SE (1997): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses. Avian Dis, 41, 627-637.
  • Jackwood DJ, Sommer SE (1998): Genetic heterogeneity in the VP2 gene of infectious bursal disease viruses detected in commercially reared chickens. Avian Dis, 42, 321-339.
  • Jackwood DJ, Sommer SE (1999): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses from outside the United States. Avian Dis, 43, 310-314.
  • Jackwood DJ, Jackwood RJ, Sommer SE (1997): Identification and comparison of point mutations associated in classic and variant infectious bursal disease viruses. Virus Res, 49, 131-137.
  • Lim BL, Cao Y, Yu T, Mo CW (1999): Adaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2. J Virol, 73, 2854-2862.
  • Lin Z, Kato A, Otaki Y, Nakamura T, Sasmaz E, Ueda S (1993): Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan. Avian Dis, 37, 315-323.
  • Liu HJ, Giambrone JJ, Dormotorio T (1994): Detection of genetic variations in serotype I isolates of infectious bursal disease virus using polymerase chain reaction and restriction endonuclease analysis. J Virol Methods, 48, 281-291.
  • Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: Calnek BW, Barnes HJ, Beard CW, McDougald LR, Saif YM (Eds.): Diseases of Poultry. 10th ed. Iowa State University Press, Ames, Iowa, USA.
  • McFerran JB, McNulty MS, McCillop E, Corner TJ, McCracken RM, Collins DS, Allan G (1980): Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys and ducks, demonstration of a second serotype. Avian Pathol, 9, 395-404.
  • Meir R, Jackwood DJ, Weisman Y (2001): Molecular typing of infectious bursal disease virus of Israeli field and vaccine strains by the reverse transcription/polymerase chain reaction/restriction fragment length polymorphism assay. Avian Dis, 45, 223-228.
  • Office International Des Epizooties (OIE) (2000): Manual of Standards for Diagnostic Tests and Vaccines. 4th Ed. OIE, Paris.
  • Pala HH, Türe O (1996): Gumboro hastalığının immunperoksidaz ve immunofloresan teknikleri ile teşhisi. Bornova Vet Kontr Araşt Enst Md Derg, 21, 161-175.
  • Rosenberger JK (1989): Infectious bursal disease. 165- 166. In: HG Purchase, LH Arp, CH Dommermuth, JE Pearson (Eds.): A Laboratory Manual for the Isolation and Identification of Avian Pathogens 3rd Ed. Kennett Square, American Association of Avian Pathologists.
  • Takase K, Uchimura T, Katsuki N, Yamamoto M (1993): Agar gel precipitin line patterns and pathogenicity of infectious bursal disease viruses. J Vet Med Sci, 55, 137-139.
  • Tham KM, Young LW, Moon CD (1995): Detection of infectious bursal disease virus by reverse-transcription- polymerase chain reaction amplification of the virus segment A gene. J Virol Meth, 53, 201-212.
  • Türe O, Saif YM, Jackwood DJ (1998): Restriction length polymorphism analysis of highly virulent strains of infectious bursal disease viruses from Holland, Turkey, and Taiwan. Avian Dis, 42, 470-479.
  • Van den Berg TP (2000): Acute infectious bursal disease in poultry: a review. Avian Pathol, 29, 175-194.
  • Van den Berg TP, Eterradossi N, Toquin D, Meulemans G (2000): Infectious Bursal Disease (Gumboro Disease). Rev Sci Tech Off İnt Epiz, 19, 527- 543.
  • Zierenberg K, Nieper H, van den Berg TP, Ezeokoli CD, Vob M, Müller H (2000): The VP2 variable region of African and German isolates of infectious bursal disease virus: comparison with very virulent, “classical” virulent, and attenuated tissue culture-adapted strains. Arch Virol, 145, 113-125.
There are 29 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Barış Sareyyüpoğlu

Müjgan İzgür

Publication Date June 1, 2006
Published in Issue Year 2006

Cite

APA Sareyyüpoğlu, B., & İzgür, M. (2006). Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 53(2), 129-136. https://doi.org/10.1501/Vetfak_0000000080
AMA Sareyyüpoğlu B, İzgür M. Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi. Ankara Univ Vet Fak Derg. June 2006;53(2):129-136. doi:10.1501/Vetfak_0000000080
Chicago Sareyyüpoğlu, Barış, and Müjgan İzgür. “Türkiye’de Saptanan infeksiyöz Bursal hastalık Virus suşlarının RFLP Ile Tiplendirilmesi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53, no. 2 (June 2006): 129-36. https://doi.org/10.1501/Vetfak_0000000080.
EndNote Sareyyüpoğlu B, İzgür M (June 1, 2006) Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53 2 129–136.
IEEE B. Sareyyüpoğlu and M. İzgür, “Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi”, Ankara Univ Vet Fak Derg, vol. 53, no. 2, pp. 129–136, 2006, doi: 10.1501/Vetfak_0000000080.
ISNAD Sareyyüpoğlu, Barış - İzgür, Müjgan. “Türkiye’de Saptanan infeksiyöz Bursal hastalık Virus suşlarının RFLP Ile Tiplendirilmesi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53/2 (June 2006), 129-136. https://doi.org/10.1501/Vetfak_0000000080.
JAMA Sareyyüpoğlu B, İzgür M. Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi. Ankara Univ Vet Fak Derg. 2006;53:129–136.
MLA Sareyyüpoğlu, Barış and Müjgan İzgür. “Türkiye’de Saptanan infeksiyöz Bursal hastalık Virus suşlarının RFLP Ile Tiplendirilmesi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 53, no. 2, 2006, pp. 129-36, doi:10.1501/Vetfak_0000000080.
Vancouver Sareyyüpoğlu B, İzgür M. Türkiye’de saptanan infeksiyöz bursal hastalık virus suşlarının RFLP ile tiplendirilmesi. Ankara Univ Vet Fak Derg. 2006;53(2):129-36.