The effect of dietary supplementation of natural antioxidants and coated calcium butyrate on carcass traits, serum biochemical parameters, lipid peroxidation in meat and intestinal histomorphology in broilers
Abstract
The aim of this study was to investigate the effects of vitamin E, grape seed extract and green tea extract with or without supplementation of coated calcium butyrate in broilers based on carcass traits, some biochemical parameters, intestinal histomorphology, and lipid peroxidation in meat. Two hundred fifty-two one-day-old broiler chicks were divided into nine groups, one control and eight experimental. Control group fed only a basal diet (control). The experimental groups' diets contained feed additives as; coated calcium butyrate (CCB), vitamin E (VitE), grape seed extract (GSE), green tea extract (GTE), a combination of vitamin E with coated calcium butyrate (VitE+CCB), grape seed extract with coated calcium butyrate (GSE+CCB), green tea extract with coated calcium butyrate (GTE+CCB), and a combination of grape seed extract, green tea extract, and coated calcium butyrate (GSE+GTE+CCB) respectively. According to the study results; no significant differences were observed in gut histomorphology and serum biochemical parameters. The broilers fed with GSE and GTE with/without CCB had significantly higher hot carcass yield than control, VitE, and VitE+CCB. Furthermore, all groups showed significantly lesser lipid peroxidation in meat than control; however, the VitE group had the lowest malondialdehyde (MDA) levels. It can be concluded that the combination of CCB with natural antioxidants could be used to improve carcass traits and meat antioxidant capacity in broilers.
Keywords
Broiler Coated calcium butyrate Intestinal histomorphology Lipid peroxidation Natural antioxidants
Supporting Institution
This research was supported by Ankara University Research Fund.
Project Number
Project No: 16L0238002
Thanks
The authors wish to acknowledge Beypi A.Ş. for chicks and Vimar A.Ş. for grape seed extract, Vitamin E and Sanita Sağlık Ürünleri A.Ş. for coated calcium butyrate supply
References
- Abu Hafsa SH, Ibrahim SA (2018): Effect of dietary polyphenol-rich grape seed on growth performance, antioxidant capacity and ileal microflora in broiler chicks. J Anim Physiol Anim Nutr, 102, 268–275.
- Aghazadeh A, Taha Yazdi M (2012): Effect of butyric acid supplementation and whole wheat inclusion on the performance and carcass traits of broilers. S Afr J Anim Sci, 42, 241–248.
- Antongiovanni M, Buccioni A, Petacchi F, et al (2007): Butyric acid glycerides in the diet of broiler chickens: effects on gut histology and carcass composition. Ital J Anim Sci, 6, 19–25.
- Augustin K, Blank R, Boesch-Saadatmandi C, et al (2008): Dietary green tea polyphenols do not affect vitamin E status, antioxidant capacity and meat quality of growing pigs. J Anim Physiol Anim Nutr, 92, 705–711.
- Bancroft JD, Gamble M (2002): Histological Techniques, 5th edition. 182-189. Churchill & Livingstone, London, Philadelphia.
- Biswas AH, Wakita M (2001): Effect of dietary japanese green tea powder supplementation on feed utilization and carcass profiles in broilers. J Poult Sci, 38, 50–57.
- Brenes A, Viveros A, Goñi I, et al (2010): Effect of grape seed extract on growth performance, protein and polyphenol digestibilities, and antioxidant activity in chickens. Span J Agric Res, 8, 326–333.
- Calık A, Ceylan A, Ekim B, et al (2017): The effect of intra-amniotic and posthatch dietary synbiotic administration on the performance, intestinal histomorphology, cecal microbial population, and short-chain fatty acid composition of broiler chickens. Poult Sci, 96, 169–183.
- Carpenter KJ, Clegg KM (1956): The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. J Sci Food Agric, 7, 45-51.
- Chamba F, Puyalto M, Ortiz A, et al (2014): Effect of partially protected sodium butyrate on performance, digestive organs, intestinal villi and E. coli development in broilers chickens. Int J Poult Sci, 13, 390–396.
- Chamorro S, Viveros A, Centeno C, et al (2013): Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks. Animal, 7, 555–561.
- Dawson B, Trapp RG (2001): Basic & Clinical Biostatistics, 3rd Edition. Lange Medical Books, McGnaw-Hill Medical Publishing Division, New York.
- Ebrahimzadeh SK, Navidshad B, Farhoomand P, et al (2018): Effects of grape pomace and vitamin E on performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens. S Afr J Anim Sci, 48, 324-336.
- Farahat M, Abdallah F, Abdel-Hamid T, et al (2016): Effect of supplementing broiler chicken diets with green tea extract on the growth performance, lipid profile, antioxidant status and immune response. Br Poult Sci, 57, 714–722.
- Farahat MH, Abdallah FM, Ali HA, et al (2017): Effect of dietary supplementation of grape seed extract on the growth performance, lipid profile, antioxidant status and immune response of broiler chickens. Animal, 11, 771–777.
- Goñi I, Brenes A, Centeno C, et al (2007): Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens. Poult Sci, 86, 508–516.
- Hajati H, Hassanabadi A, Golian AG, et al (2015): The effect of grape seed extract and vitamin C feed supplements carcass characteristics, gut morphology and ileal microflora in broiler chickens exposed to chronic heat stress. Iran J Appl Anim Sci, 5, 155–165.
- Hassan FA, Mahrose KM, Basyony MM (2014): Influence of grape seeds powder as a natural antioxidant on growth performance, antioxidant status and carass characteristics of rabbits under hot conditions. 395–412. In: The 7th International Conference on Rabbit Production in Hot Climate, Egypt.
- Hassanpour H, Zamani Moghaddam AK, Yazdani A, et al (2010): Evaluation of intestinal morphology and nitric oxide metabolites in broiler chickens supplemented by green tea. Comp Clin Path, 19, 43–47.
- Jiang Y, Zhang WH, Gao F, et al (2015): Micro-encapsulated sodium butyrate attenuates oxidative stress induced by corticosterone exposure and modulates apoptosis in intestinal mucosa of broiler chickens. Anim Prod Sci, 55, 587–594.
- Leeson S, Namkung H, Antongiovanni M, et al (2005): Effect of butyric acid on the performance and carcass yield of broiler chickens. Poult Sci, 84, 1418–1422.
- Mallo JJ, Puyalto M, Rama Rao SV (2012): Evaluation of the effect of sodium butyrate addition to broilers diet on energy and protein digestibility, productive parameters and size of intestinal villi of animals. Feed Livest, 8, 26–30.
- National Research Council (1994): Nutrient Requirements of Poultry: 9th Revised Edition, 1994. National Academies Press, Washington.
- Panda AK, Rao SVR, Raju MVLN, et al (2009): Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australas J Anim Sci, 22, 1026–1031.
- Rietjens IMCM, Boersma MG, Haan L de, et al (2002): The pro-oxidant chemistry of the natural antioxidants vitamin C, vitamin E, carotenoids and flavonoids. Environ Toxicol Pharmacol, 11, 321–333.
- Rowghani E, Tabeidian SA, Abolfathi E (2016): The effects of green tea extract and vitamin E on the growth performance and immune response in broiler chicks. Res Opin Anim Vet Sci, 6, 200–205.
- Sarıca Ş, Ürkmez D (2016): The use of grape seed-, olive leaf- and pomegranate peel-extracts as alternative natural antimicrobial feed additives in broiler diets. Europ Poult Sci, 80, 1–13.
- Sayrafi R, Soltanalinejad F, Shahrooz R, et al (2011): Effects of butyric acid glycerides and antibiotic growth promoter on the performance and intestinal histomorphometry of broiler chickens. J Food Agric Environ, 9, 285–288.
- Smet K, Raes K, Huyghebaert G, et al (2005): Influence of feed enriched with natural antioxidants on the oxidative stability of broiler meat. 99–106. In: Proc. 17th European Symposium on the Quality of Poultry Meat, Doorwerth, Doorwerth, The Netherlands.
- Taherpour K, Moravej H, Shivazad M, et al (2009): Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. Afr J Biotechnol, 8, 2329–2334.
- Van Den Borne JJGC, Heetkamp MJW, Buyse J, et al (2015): Fat coating of Ca butyrate results in extended butyrate release in the gastrointestinal tract of broilers. Livest Sci, 175, 96–100.
- Villar-Patino G, Diaz-Cruz A, Avila-Gonzalez E, et al (2002): Effects of dietary supplementation with vitamin C or vitamin E on cardiac lipid peroxidation and growth performance in broilers at risk of developing ascites syndrome. Am J Vet Res, 63, 673–676.
- Viveros A, Chamorro S, Pizarro M, et al (2011): Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult Sci, 90, 566–578.
- Vlaicu PA, Saracila M, Panaite TD, et al (2017): Effect of the dietary grape seeds and rosehip oils given to broilers (14-42 days) reared at 32°C on broiler performance, relative weight of carcass cuts and internal organs and balance of gut microflora. Arch Zootech, 20, 77–88.
- Vossen E, Ntawubizi M, Raes K, et al (2011): Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers: Dietary antioxidants and oxidative status in broilers. J Anim Physiol Anim Nutr, 95, 198–205.
- Wu Y, Zhou Y, Lu C, et al (2016): Influence of butyrate loaded clinoptilolite dietary supplementation on growth performance, development of intestine and antioxidant capacity in broiler chickens. PLoS One, 11, e0154410.
- Yin F, Yu H, Lepp D, et al (2016): Transcriptome analysis reveals regulation of gene expression for lipid catabolism in young broilers by butyrate glycerides. PLoS One, 11, e0160751.
- Yoo J, Yi YJ, Koo B, et al (2016): Growth performance, intestinal morphology, and meat quality in relation to alpha-lipoic acid associated with vitamin C and E in broiler chickens under tropical conditions. RBZ, 45, 113-120.
- Zdanowska-Sąsiadek Ż, Michalczuk M, Damaziak K, et al (2016): Effect of vitamin E supplementation on growth performance and chicken meat quality. Europ Poult Sci, 80, 1–14.
- Zhang WH, Jiang Y, Zhu QF, et al (2011): Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br Poult Sci, 52, 292–301
Etçi piliç rasyonlarına doğal antioksidanlar ve kaplanmış kalsiyum bütirat ilavesinin karkas özellikleri, serum biyokimyasal parametreleri, ette lipid peroksidasyon ve bağırsak histomorfolojisi üzerine etkileri
Abstract
Bu çalışmanın amacı, vitamin E, üzüm çekirdeği ekstraktı ve yeşil çay ekstraktının tek başına veya kaplanmış kalsiyum bütirat ile birlikte kullanılmasının etçi piliçlerde karkas özellikleri, bazı biyokimyasal parametreleri, bağırsak morfolojisi ve ette lipid peroksidasyon düzeyi üzerindeki etkisini araştırmaktır. Bir günlük 252 civciv, bir kontrol ve sekiz deneme grubu olmak üzere dokuz gruba ayrılmıştır. Kontrol grubuna sadece temel rasyon verilmiştir (Kontrol). Deneme gruplarının yemleri sırasıyla kaplanmış kalsiyum bütirat (CCB), vitamin E (VitE), üzüm çekirdeği ekstraktı (GSE), yeşil çay ekstraktı (GTE), vitamin E ile kaplanmış kalsiyum bütirat (VitE+CCB), üzüm çekirdeği ekstraktı ile kaplanmış kalsiyum bütirat (GSE+CCB), yeşil çay ekstraktı ile kaplanmış kalsiyum bütirat (GTE+CCB) ve üzüm çekirdeği + kaplanmış kalsiyum bütirat yeşil çay ekstraktının (GSE+GTE+CCB) birlikte olduğu yem katkılarını içermektedir. Çalışma sonuçlarına göre; bağırsak histomorfolojisi ve serum biyokimyasal parametreler açısından gruplar arasında anlamlı bir fark tespit edilememiştir. GSE ve GTE tek başına veya CCB ile verildiği etçi piliçlerde, Kontrol, VitE ve VitE+CCB kombinasyonu verilen gruplara göre sıcak karkas randımanın anlamlı derecede yüksek olduğu gözlenmiştir. Ayrıca, bütün gruplar kontrol grubuna göre ette önemli düzeyde daha düşük lipid peroksidasyonu gösterdi, fakat VitE grubu en düşük MDA seviyesine sahipti. Sonuç olarak etçi piliçlerde CCB’nin doğal antioksidanlarla kombine olarak kullanılmasının sıcak karkas verimi ve et antioksidan kapasitesini iyileştirmek için kullanılabileceği sonucuna varılabilmektedir.
Keywords
Etçi piliç bağırsak histomorfolojisi doğal antioksidanlar kaplanmış kalsiyum bütirat lipid peroksidasyon
Project Number
Project No: 16L0238002
References
- Abu Hafsa SH, Ibrahim SA (2018): Effect of dietary polyphenol-rich grape seed on growth performance, antioxidant capacity and ileal microflora in broiler chicks. J Anim Physiol Anim Nutr, 102, 268–275.
- Aghazadeh A, Taha Yazdi M (2012): Effect of butyric acid supplementation and whole wheat inclusion on the performance and carcass traits of broilers. S Afr J Anim Sci, 42, 241–248.
- Antongiovanni M, Buccioni A, Petacchi F, et al (2007): Butyric acid glycerides in the diet of broiler chickens: effects on gut histology and carcass composition. Ital J Anim Sci, 6, 19–25.
- Augustin K, Blank R, Boesch-Saadatmandi C, et al (2008): Dietary green tea polyphenols do not affect vitamin E status, antioxidant capacity and meat quality of growing pigs. J Anim Physiol Anim Nutr, 92, 705–711.
- Bancroft JD, Gamble M (2002): Histological Techniques, 5th edition. 182-189. Churchill & Livingstone, London, Philadelphia.
- Biswas AH, Wakita M (2001): Effect of dietary japanese green tea powder supplementation on feed utilization and carcass profiles in broilers. J Poult Sci, 38, 50–57.
- Brenes A, Viveros A, Goñi I, et al (2010): Effect of grape seed extract on growth performance, protein and polyphenol digestibilities, and antioxidant activity in chickens. Span J Agric Res, 8, 326–333.
- Calık A, Ceylan A, Ekim B, et al (2017): The effect of intra-amniotic and posthatch dietary synbiotic administration on the performance, intestinal histomorphology, cecal microbial population, and short-chain fatty acid composition of broiler chickens. Poult Sci, 96, 169–183.
- Carpenter KJ, Clegg KM (1956): The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. J Sci Food Agric, 7, 45-51.
- Chamba F, Puyalto M, Ortiz A, et al (2014): Effect of partially protected sodium butyrate on performance, digestive organs, intestinal villi and E. coli development in broilers chickens. Int J Poult Sci, 13, 390–396.
- Chamorro S, Viveros A, Centeno C, et al (2013): Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks. Animal, 7, 555–561.
- Dawson B, Trapp RG (2001): Basic & Clinical Biostatistics, 3rd Edition. Lange Medical Books, McGnaw-Hill Medical Publishing Division, New York.
- Ebrahimzadeh SK, Navidshad B, Farhoomand P, et al (2018): Effects of grape pomace and vitamin E on performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens. S Afr J Anim Sci, 48, 324-336.
- Farahat M, Abdallah F, Abdel-Hamid T, et al (2016): Effect of supplementing broiler chicken diets with green tea extract on the growth performance, lipid profile, antioxidant status and immune response. Br Poult Sci, 57, 714–722.
- Farahat MH, Abdallah FM, Ali HA, et al (2017): Effect of dietary supplementation of grape seed extract on the growth performance, lipid profile, antioxidant status and immune response of broiler chickens. Animal, 11, 771–777.
- Goñi I, Brenes A, Centeno C, et al (2007): Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens. Poult Sci, 86, 508–516.
- Hajati H, Hassanabadi A, Golian AG, et al (2015): The effect of grape seed extract and vitamin C feed supplements carcass characteristics, gut morphology and ileal microflora in broiler chickens exposed to chronic heat stress. Iran J Appl Anim Sci, 5, 155–165.
- Hassan FA, Mahrose KM, Basyony MM (2014): Influence of grape seeds powder as a natural antioxidant on growth performance, antioxidant status and carass characteristics of rabbits under hot conditions. 395–412. In: The 7th International Conference on Rabbit Production in Hot Climate, Egypt.
- Hassanpour H, Zamani Moghaddam AK, Yazdani A, et al (2010): Evaluation of intestinal morphology and nitric oxide metabolites in broiler chickens supplemented by green tea. Comp Clin Path, 19, 43–47.
- Jiang Y, Zhang WH, Gao F, et al (2015): Micro-encapsulated sodium butyrate attenuates oxidative stress induced by corticosterone exposure and modulates apoptosis in intestinal mucosa of broiler chickens. Anim Prod Sci, 55, 587–594.
- Leeson S, Namkung H, Antongiovanni M, et al (2005): Effect of butyric acid on the performance and carcass yield of broiler chickens. Poult Sci, 84, 1418–1422.
- Mallo JJ, Puyalto M, Rama Rao SV (2012): Evaluation of the effect of sodium butyrate addition to broilers diet on energy and protein digestibility, productive parameters and size of intestinal villi of animals. Feed Livest, 8, 26–30.
- National Research Council (1994): Nutrient Requirements of Poultry: 9th Revised Edition, 1994. National Academies Press, Washington.
- Panda AK, Rao SVR, Raju MVLN, et al (2009): Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australas J Anim Sci, 22, 1026–1031.
- Rietjens IMCM, Boersma MG, Haan L de, et al (2002): The pro-oxidant chemistry of the natural antioxidants vitamin C, vitamin E, carotenoids and flavonoids. Environ Toxicol Pharmacol, 11, 321–333.
- Rowghani E, Tabeidian SA, Abolfathi E (2016): The effects of green tea extract and vitamin E on the growth performance and immune response in broiler chicks. Res Opin Anim Vet Sci, 6, 200–205.
- Sarıca Ş, Ürkmez D (2016): The use of grape seed-, olive leaf- and pomegranate peel-extracts as alternative natural antimicrobial feed additives in broiler diets. Europ Poult Sci, 80, 1–13.
- Sayrafi R, Soltanalinejad F, Shahrooz R, et al (2011): Effects of butyric acid glycerides and antibiotic growth promoter on the performance and intestinal histomorphometry of broiler chickens. J Food Agric Environ, 9, 285–288.
- Smet K, Raes K, Huyghebaert G, et al (2005): Influence of feed enriched with natural antioxidants on the oxidative stability of broiler meat. 99–106. In: Proc. 17th European Symposium on the Quality of Poultry Meat, Doorwerth, Doorwerth, The Netherlands.
- Taherpour K, Moravej H, Shivazad M, et al (2009): Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. Afr J Biotechnol, 8, 2329–2334.
- Van Den Borne JJGC, Heetkamp MJW, Buyse J, et al (2015): Fat coating of Ca butyrate results in extended butyrate release in the gastrointestinal tract of broilers. Livest Sci, 175, 96–100.
- Villar-Patino G, Diaz-Cruz A, Avila-Gonzalez E, et al (2002): Effects of dietary supplementation with vitamin C or vitamin E on cardiac lipid peroxidation and growth performance in broilers at risk of developing ascites syndrome. Am J Vet Res, 63, 673–676.
- Viveros A, Chamorro S, Pizarro M, et al (2011): Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult Sci, 90, 566–578.
- Vlaicu PA, Saracila M, Panaite TD, et al (2017): Effect of the dietary grape seeds and rosehip oils given to broilers (14-42 days) reared at 32°C on broiler performance, relative weight of carcass cuts and internal organs and balance of gut microflora. Arch Zootech, 20, 77–88.
- Vossen E, Ntawubizi M, Raes K, et al (2011): Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers: Dietary antioxidants and oxidative status in broilers. J Anim Physiol Anim Nutr, 95, 198–205.
- Wu Y, Zhou Y, Lu C, et al (2016): Influence of butyrate loaded clinoptilolite dietary supplementation on growth performance, development of intestine and antioxidant capacity in broiler chickens. PLoS One, 11, e0154410.
- Yin F, Yu H, Lepp D, et al (2016): Transcriptome analysis reveals regulation of gene expression for lipid catabolism in young broilers by butyrate glycerides. PLoS One, 11, e0160751.
- Yoo J, Yi YJ, Koo B, et al (2016): Growth performance, intestinal morphology, and meat quality in relation to alpha-lipoic acid associated with vitamin C and E in broiler chickens under tropical conditions. RBZ, 45, 113-120.
- Zdanowska-Sąsiadek Ż, Michalczuk M, Damaziak K, et al (2016): Effect of vitamin E supplementation on growth performance and chicken meat quality. Europ Poult Sci, 80, 1–14.
- Zhang WH, Jiang Y, Zhu QF, et al (2011): Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br Poult Sci, 52, 292–301
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | Project No: 16L0238002 |
| Publication Date | June 30, 2021 |
| Published in Issue | Year 2021 |