Research Article
Year 2021,
, 129 - 135, 31.03.2021
Abstract
This study was carried out between November-2017 and June-2019 to determine the distribution and molecular characterization of Gongylonema pulchrum in cattle and sheep slaughtered in local abattoirs and slaughtering areas during the feast of sacrifice. In this study, esophagi collected from 380 (358 young and 22 mature) cattle and 848 esophagi from sheep (816 young and 32 old) were examined. Esophagus taken after slaughtering was brought to the laboratory in plastic bag. In the laboratory, all the esophagi were cut open with scissors and all of the materials were examined using a loupe under a light source. Parasites were taken out using eye forceps and placed in 70% ethyl alcohol. Following the primary morphological examination, all the parasites cut into three parts, anterior and posterior parts were used for morphologic identification and mid part was used for molecular identification. Bp BpCoxI-F1 and BpCoxI-R1 primers were used for molecular identification. Only two cows (older than four years) were infected and the infection rate was 0.53%. A total of 27 parasites were identified, 18 were male and 9 were female. Parasites were not found in the examined sheep. All the samples collected from the infected cattle were identified as G. pulchrum after morphological examinations and molecular confirmation and positive bands were detected at 400bp.
Keywords
Supporting Institution
Scientific Project Office of Ondokuz Mayıs University
Project Number
PYO.VET.1904.16.009
References
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- Anwar M, Rak H, Gyorkos TW (1979): The incidence of Gongylonema pulchrum from cattle in Tehran, Iran. Vet Parasitol, 5, 271-274.
- Celep A (1987): Samsun yöresi kuzu ve toklularda paraziter fauna tesbiti ile kontrol ve tedavi gruplarında aylık ortalama ağırlık artışlarının belirlenmesine dair araştırmalar. Vet Hekim Dern Derg, 57, 69-79.
- Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 8, 46-57.
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- Değer S, Biçek K (2005): Van ve yöresinde koyunlarda endoparaziter fauna tespiti ve paraziter invazyonların kontrolü üzerine öneriler. YYÜ Vet Fak Derg, 16, 51-54.
- Doğanay A, Öge S (1997): Türkiye’de koyun ve keçilerde görülen helmintler. Kafkas Üniv Vet Fak Derg, 3, 97-114.
- Eslami A, Ashrafihelan J, Vahedi N (2010): Study on the prevalence and pathology of Gongylonema pulchrum (Gullet worm) of sheep from Iran. Global Vet, 5, 45-48.
- Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol, 52, 696-704.
- Gül A (2008): Prevalence of Gongylonema pulchrum in sheep. Indian Vet J, 85, 1241.
- Halajian A, Eslami A, Salehi N, et al (2010): Incidence and genetic characterization of Gongylonema pulchrum in cattle slaughtered in Mazandaran province, Northern Iran. Iranian J Parasitol, 5, 10-18.
- Hall T (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In Nucleic Acids Symposium Series, 41, 95-98.
- Huang Q, Wang J, Yang T, et al (2016): Multiple Gongylonema pulchrum worms in a human esophagus. Endoscopy, 48, E24–E25.
- Kheirandish R, Radfar MH, Sharifi H, et al (2013): Prevalence and pathology of Gongylonema pulchrum in cattle slaughtered in Rudsar, northern Iran. Sci Parasitol, 14, 37-42.
- Kinsella JM, Robles MR, Preisser WC (2016): A review of Gongylonema spp. (Nematoda: Gongylonematidae) in North American rodents with description of a new species from the cotton rat, Sigmodon hispidus (Mammalia: Cricetidae). Zootaxa, 4107, 277-284.
- Kumar S, Stecher G, Li M, et al (2018): Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol, 35, 1547-1549.
- Liu GH, Jia YQ, Wang YN, et al (2015): The complete mitochondrial genome of the gullet worm Gongylonema pulchrum: gene content, arrangement, composition and phylogenetic implications. Parasit Vectors, 8, 100.
- Makouloutou P, Rana HB, Adhikari B, et al (2013): A distinct genetic population of Gongylonema pulchrum from water buffaloes in Nepal. J Parasitol, 99, 669–676.
- Makouloutou P, Setsuda A, Yokoyama M, et al (2013): Genetic variation of Gongylonema pulchrum from wild animals and cattle in Japan based on ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. J Helminthol, 87, 326-335.
- Merdivenci A (1983): Son 30 yıl içinde Türkiye’de varlığını ilk kez bildirdiğimiz parazitler. Türk Mikrobiyol Cem Derg, 13, 23-27.
- Naem S, Gorgani T (2011): Gastrointestinal parasitic infection of slaughtered sheep (Zel breed) in Fereidoonkenar city, Iran. Vet Res Forum, 2, 238-241.
- Oğuz T (1970): Gongylonema pulchrum Molin, 1857’nin morfolojisi ile Ankara civarındaki arakonakcılarına ait araştırmalar. Ankara Univ Vet Fak Derg, 17, 136-155.
- Papini R, Cecchi V, Capocchi O, et al (2013): Small ruminant Gongylonema pulchrum infection in the South West Algerian desert: prevalence of a sporadic zoonosis. Med Weter, 69, 161-164.
- Posada D (2008): jModelTest: phylogenetic model averaging. Mol Biol Evol, 25, 1253-1256.
- Rozas J, Ferrer-Mata A, Sánchez-Delbarrio JC, et al (2017): DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Mol Biol Evol, 34, 3299-3302.
- Setsuda A, Da N, Hasegawa H, et al (2016): Intraspecific and interspecific genetic variation of Gongylonema pulchrum and two rodent Gongylonema spp. (G. aegypti and G. neoplasticum), with the proposal of G. nepalensis n. sp. for the isolate in water buffaloes from Nepal. Parasitol Res, 115, 787-795.
- Senlik B, Cirak VY, Girisgin O, et al (2011): Helminth infections of wild boars (Sus scrofa) in the Bursa province of Turkey. J Helminthol, 85, 404-408.
- Tamura K, Nei M, Kumar S (2004): Prospects for inferring very large phylogenies by using the neighbour-joining method. Proc Natl Acad Sci, 101, 11030-11035.
- Tüzdil AN (1939): Türkiye Kasaplık Hayvanlarında Gongylonema. Ankara. Yüksek Ziraat Enst. Ankara.
- Umur Ş, Köroğlu E, Güçlü F, et al (2011): Nematoda. 353-355. In. Tınar R. (Ed). Veteriner Helmintoloji. Dora Basın, Yayın, Dağıtım. Bursa.
- Varcasia A, Scala A, Zidda A, et al (2017): First record of Gongylonema nepalensis in domestic and wild ruminants in Europe. Vet Parasitol, 246, 11-18.
- Xiaodan L, Zhensheng W, Ying H, et al (2018): Gongylonema pulchrum infection in the human oral cavity: A case report and literature review. Oral Surg Oral Med Oral Pathol Oral Radiol, 12125, e49–e53.
Year 2021,
, 129 - 135, 31.03.2021
Abstract
Project Number
PYO.VET.1904.16.009
References
- Altschul SF, Madden TL, Schaffer AA, et al (1997): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res, 25, 3389-3402.
- Aldemir OS, Güçlü F, Akça A (2004): Kars yöresi koç ve koyunlarında Gongylonema pulchrum’un yayılışı. Türkiye Parazitol Derg, 28, 96-99.
- Anwar M, Rak H, Gyorkos TW (1979): The incidence of Gongylonema pulchrum from cattle in Tehran, Iran. Vet Parasitol, 5, 271-274.
- Celep A (1987): Samsun yöresi kuzu ve toklularda paraziter fauna tesbiti ile kontrol ve tedavi gruplarında aylık ortalama ağırlık artışlarının belirlenmesine dair araştırmalar. Vet Hekim Dern Derg, 57, 69-79.
- Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 8, 46-57.
- Cordeiro HC, Melo FTV, Giese EG, et al (2018): Gongylonema parasites of rodents: A key to species and new data on Gongylonema neoplasticum. J Parasitol, 104, 51-59.
- Değer S, Biçek K (2005): Van ve yöresinde koyunlarda endoparaziter fauna tespiti ve paraziter invazyonların kontrolü üzerine öneriler. YYÜ Vet Fak Derg, 16, 51-54.
- Doğanay A, Öge S (1997): Türkiye’de koyun ve keçilerde görülen helmintler. Kafkas Üniv Vet Fak Derg, 3, 97-114.
- Eslami A, Ashrafihelan J, Vahedi N (2010): Study on the prevalence and pathology of Gongylonema pulchrum (Gullet worm) of sheep from Iran. Global Vet, 5, 45-48.
- Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol, 52, 696-704.
- Gül A (2008): Prevalence of Gongylonema pulchrum in sheep. Indian Vet J, 85, 1241.
- Halajian A, Eslami A, Salehi N, et al (2010): Incidence and genetic characterization of Gongylonema pulchrum in cattle slaughtered in Mazandaran province, Northern Iran. Iranian J Parasitol, 5, 10-18.
- Hall T (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In Nucleic Acids Symposium Series, 41, 95-98.
- Huang Q, Wang J, Yang T, et al (2016): Multiple Gongylonema pulchrum worms in a human esophagus. Endoscopy, 48, E24–E25.
- Kheirandish R, Radfar MH, Sharifi H, et al (2013): Prevalence and pathology of Gongylonema pulchrum in cattle slaughtered in Rudsar, northern Iran. Sci Parasitol, 14, 37-42.
- Kinsella JM, Robles MR, Preisser WC (2016): A review of Gongylonema spp. (Nematoda: Gongylonematidae) in North American rodents with description of a new species from the cotton rat, Sigmodon hispidus (Mammalia: Cricetidae). Zootaxa, 4107, 277-284.
- Kumar S, Stecher G, Li M, et al (2018): Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol, 35, 1547-1549.
- Liu GH, Jia YQ, Wang YN, et al (2015): The complete mitochondrial genome of the gullet worm Gongylonema pulchrum: gene content, arrangement, composition and phylogenetic implications. Parasit Vectors, 8, 100.
- Makouloutou P, Rana HB, Adhikari B, et al (2013): A distinct genetic population of Gongylonema pulchrum from water buffaloes in Nepal. J Parasitol, 99, 669–676.
- Makouloutou P, Setsuda A, Yokoyama M, et al (2013): Genetic variation of Gongylonema pulchrum from wild animals and cattle in Japan based on ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. J Helminthol, 87, 326-335.
- Merdivenci A (1983): Son 30 yıl içinde Türkiye’de varlığını ilk kez bildirdiğimiz parazitler. Türk Mikrobiyol Cem Derg, 13, 23-27.
- Naem S, Gorgani T (2011): Gastrointestinal parasitic infection of slaughtered sheep (Zel breed) in Fereidoonkenar city, Iran. Vet Res Forum, 2, 238-241.
- Oğuz T (1970): Gongylonema pulchrum Molin, 1857’nin morfolojisi ile Ankara civarındaki arakonakcılarına ait araştırmalar. Ankara Univ Vet Fak Derg, 17, 136-155.
- Papini R, Cecchi V, Capocchi O, et al (2013): Small ruminant Gongylonema pulchrum infection in the South West Algerian desert: prevalence of a sporadic zoonosis. Med Weter, 69, 161-164.
- Posada D (2008): jModelTest: phylogenetic model averaging. Mol Biol Evol, 25, 1253-1256.
- Rozas J, Ferrer-Mata A, Sánchez-Delbarrio JC, et al (2017): DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Mol Biol Evol, 34, 3299-3302.
- Setsuda A, Da N, Hasegawa H, et al (2016): Intraspecific and interspecific genetic variation of Gongylonema pulchrum and two rodent Gongylonema spp. (G. aegypti and G. neoplasticum), with the proposal of G. nepalensis n. sp. for the isolate in water buffaloes from Nepal. Parasitol Res, 115, 787-795.
- Senlik B, Cirak VY, Girisgin O, et al (2011): Helminth infections of wild boars (Sus scrofa) in the Bursa province of Turkey. J Helminthol, 85, 404-408.
- Tamura K, Nei M, Kumar S (2004): Prospects for inferring very large phylogenies by using the neighbour-joining method. Proc Natl Acad Sci, 101, 11030-11035.
- Tüzdil AN (1939): Türkiye Kasaplık Hayvanlarında Gongylonema. Ankara. Yüksek Ziraat Enst. Ankara.
- Umur Ş, Köroğlu E, Güçlü F, et al (2011): Nematoda. 353-355. In. Tınar R. (Ed). Veteriner Helmintoloji. Dora Basın, Yayın, Dağıtım. Bursa.
- Varcasia A, Scala A, Zidda A, et al (2017): First record of Gongylonema nepalensis in domestic and wild ruminants in Europe. Vet Parasitol, 246, 11-18.
- Xiaodan L, Zhensheng W, Ying H, et al (2018): Gongylonema pulchrum infection in the human oral cavity: A case report and literature review. Oral Surg Oral Med Oral Pathol Oral Radiol, 12125, e49–e53.
There are 33 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | PYO.VET.1904.16.009 |
| Publication Date | March 31, 2021 |
| Published in Issue | Year 2021 |