Research Article
BibTex RIS Cite

Year 2021, , 223 - 228, 30.06.2021

Yusuf Padak Ayşe Karakuş

https://doi.org/10.33988/auvfd.727842
Cited By: 3

Abstract

References

  • Anderson GR, Barker SC (1998): Inference of phylogeny and taxonomy within the didymozoidae (Digenea) from the second internal transcribed spacer ITS2 of ribosomal DNA. Syst Parasitol, 41, 87-94.
  • Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 6, 117-30.
  • Coşkun ŞZ (1988): The discovery and spread of Paramphistomum species in ruminants.Turk J Vet Anim Sci, 12, 168-179.
  • Goswami LM, Prasad PK, Tandon V, et al (2009): Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis. Parasitol Res, 104, 1485-1490.
  • Güralp N (1981): The Helminthology. Ankara University Faculty of Veterinary Medicine. Ankara University Press, Ankara, Turkey.
  • Horak IG (1971): Paramphistomiasis of domestic ruminants. Adv Parasitol, 9, 33-72.
  • Ichikawa M, Kondoh D, Bawn S, et al (2013): Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. JVMS, 75, 309-314.
  • Itagaki T, Tsumagari N, Tsutsumi K, et al (2003): Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci, 65, 931-933.
  • Jones A (1990): Techniques for hand-sectioning thickbodied Platyhelminths. Syst Parasitol, 15, 211-218.
  • Khan UJ (2008): Epidemiology, Economic Importance and Therapy of Paramphistomosis in Cattle and Buffaloes. PhD Thesis Department of zoology, University of Punjap, Lahor, Pakistan.
  • Lotfy WM, Brant SV, Ashmawy KI, et al (2010): A molecular approach for identification of paramphistomes from Africa and Asia. Vet Parasitol, 174, 234-240.
  • Lotfy WM, Brant SV, DeJong RJ, et al (2008): Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). Am J Trop Med Hyg, 79, 248-255.
  • Luton K, Walker D, Blair D (1992): Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Piatyhelminthes: Trematoda: Digenea). Mol Biochem Parasit, 56, 323-328.
  • Martínez-Ibeas AM, González-Warleta M, Martínez-Valladares M, et al (2013): Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species. BMC Vet Res, 12, 143.
  • Özdal N, Gül A, İlhan F, et al (2010): Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey. Helminthologia, 47, 20-24.
  • Reinitz DM, Yoshino TP, Cole RA (2007): A Ribeiroia spp. (Class: Trematoda)-specific PCR-based diagnostic. J. Parasitol, 93, 1234-1238.
  • Rinaldi L, Perugini AG, Capuano F, et al (2005): Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy. Vet Parasitol, 131, 247-253.
  • Roy B, Tandon V (1993): Morphological and microtopographical train variations among Fasciolopsis buski originating from different geographical areas. Acta Parasitol, 38, 72-77.
  • Sanabria R, More G, Romero J (2011): Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae). Vet Parasitol, 177, 182-185.
  • Şenlik B (2013): Helminth Diseases Seen in Digestive System in Cattle. In: Ozcel MA (Ed). Parasitic Diseases in Veterinary Medicine. Meta Printing Printing Services. İzmir.
  • Sharma S, Lyngdoh D, Roy B, et al (2016): Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitol Res, 5, 4293-4298.
  • Sharma S, Lyngdoh D, Roy B, et al (2016): Molecular phylogeny of Cyclophyllidea (Cestoda:Eucestoda): An insilico analysis based on mtCOI gene. Parasitol Res, 115, 3329-3335.
  • Sindičić M, Martinkovic F, Striskovic T, et al (2017): Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus. J Helminthol, 91, 637-641.
  • Tandon V, Roy B (2002): SEM Pictorial Guide to Trematodes of Live Stock In India. Regency Publication. New Delhi, India.
  • Thompson RCA, Zarlenga DS, La Rosa G (2004): Molecular systematics and diagnosis. Vet Parasitol, 125, 69-92.
  • Tınar R, Umur Ş, Köroğlu E, et al (2006): The Helmintology. In: Tınar R (Ed). Nobel, Ankara, Turkey.
  • Titi A (2013): La paramphistomose gastro-duodenales des ruminants dans le nord-est algerien: investigations sur les bovins et le mollusque hote PhD thesis, Constantine University, Algeria.
  • Van Van K, Dalsgaard A, Blair D, et al (2009): Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adults and larvae. Exp Parasitol, 123, 146-151.

Molecular identification of Paramphistomidae obtained from ruminants in Van province

Year 2021, , 223 - 228, 30.06.2021

Yusuf Padak Ayşe Karakuş

https://doi.org/10.33988/auvfd.727842
Cited By: 3

Abstract

This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecular methods between March 2018 and September 2018 at Van municipality slaughterhouse. In the research, the rDNA ITS-2 gene region of adult Paramphistomums was amplified by PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result of bidirectional sequence analysis made from PCR amplicons, Paramphistomum leydeni and Calicophoron daubneyi species were identifyed. Amplicon sequences were compared by BLAST with reference sequences from Genbank. Phylogenetic tree was created with the Neighbor-Joining method by using the MEGA 7 program. Genotypes obtained from isolates were compared for exact or closest similarities. In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, it was identified for the first time by using molecular methods. Also in this study, P. leydeni was reported for the first time in Turkey.

Keywords

Molecular identification Paramphistomum spp., PCR Van

References

  • Anderson GR, Barker SC (1998): Inference of phylogeny and taxonomy within the didymozoidae (Digenea) from the second internal transcribed spacer ITS2 of ribosomal DNA. Syst Parasitol, 41, 87-94.
  • Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 6, 117-30.
  • Coşkun ŞZ (1988): The discovery and spread of Paramphistomum species in ruminants.Turk J Vet Anim Sci, 12, 168-179.
  • Goswami LM, Prasad PK, Tandon V, et al (2009): Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis. Parasitol Res, 104, 1485-1490.
  • Güralp N (1981): The Helminthology. Ankara University Faculty of Veterinary Medicine. Ankara University Press, Ankara, Turkey.
  • Horak IG (1971): Paramphistomiasis of domestic ruminants. Adv Parasitol, 9, 33-72.
  • Ichikawa M, Kondoh D, Bawn S, et al (2013): Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. JVMS, 75, 309-314.
  • Itagaki T, Tsumagari N, Tsutsumi K, et al (2003): Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci, 65, 931-933.
  • Jones A (1990): Techniques for hand-sectioning thickbodied Platyhelminths. Syst Parasitol, 15, 211-218.
  • Khan UJ (2008): Epidemiology, Economic Importance and Therapy of Paramphistomosis in Cattle and Buffaloes. PhD Thesis Department of zoology, University of Punjap, Lahor, Pakistan.
  • Lotfy WM, Brant SV, Ashmawy KI, et al (2010): A molecular approach for identification of paramphistomes from Africa and Asia. Vet Parasitol, 174, 234-240.
  • Lotfy WM, Brant SV, DeJong RJ, et al (2008): Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). Am J Trop Med Hyg, 79, 248-255.
  • Luton K, Walker D, Blair D (1992): Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Piatyhelminthes: Trematoda: Digenea). Mol Biochem Parasit, 56, 323-328.
  • Martínez-Ibeas AM, González-Warleta M, Martínez-Valladares M, et al (2013): Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species. BMC Vet Res, 12, 143.
  • Özdal N, Gül A, İlhan F, et al (2010): Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey. Helminthologia, 47, 20-24.
  • Reinitz DM, Yoshino TP, Cole RA (2007): A Ribeiroia spp. (Class: Trematoda)-specific PCR-based diagnostic. J. Parasitol, 93, 1234-1238.
  • Rinaldi L, Perugini AG, Capuano F, et al (2005): Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy. Vet Parasitol, 131, 247-253.
  • Roy B, Tandon V (1993): Morphological and microtopographical train variations among Fasciolopsis buski originating from different geographical areas. Acta Parasitol, 38, 72-77.
  • Sanabria R, More G, Romero J (2011): Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae). Vet Parasitol, 177, 182-185.
  • Şenlik B (2013): Helminth Diseases Seen in Digestive System in Cattle. In: Ozcel MA (Ed). Parasitic Diseases in Veterinary Medicine. Meta Printing Printing Services. İzmir.
  • Sharma S, Lyngdoh D, Roy B, et al (2016): Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitol Res, 5, 4293-4298.
  • Sharma S, Lyngdoh D, Roy B, et al (2016): Molecular phylogeny of Cyclophyllidea (Cestoda:Eucestoda): An insilico analysis based on mtCOI gene. Parasitol Res, 115, 3329-3335.
  • Sindičić M, Martinkovic F, Striskovic T, et al (2017): Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus. J Helminthol, 91, 637-641.
  • Tandon V, Roy B (2002): SEM Pictorial Guide to Trematodes of Live Stock In India. Regency Publication. New Delhi, India.
  • Thompson RCA, Zarlenga DS, La Rosa G (2004): Molecular systematics and diagnosis. Vet Parasitol, 125, 69-92.
  • Tınar R, Umur Ş, Köroğlu E, et al (2006): The Helmintology. In: Tınar R (Ed). Nobel, Ankara, Turkey.
  • Titi A (2013): La paramphistomose gastro-duodenales des ruminants dans le nord-est algerien: investigations sur les bovins et le mollusque hote PhD thesis, Constantine University, Algeria.
  • Van Van K, Dalsgaard A, Blair D, et al (2009): Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adults and larvae. Exp Parasitol, 123, 146-151.
There are 28 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Yusuf Padak 0000-0002-5785-9129

Ayşe Karakuş 0000-0002-7151-8777

Publication Date June 30, 2021
Published in Issue Year 2021

Cite

APA Padak, Y., & Karakuş, A. (2021). Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(3), 223-228. https://doi.org/10.33988/auvfd.727842
AMA Padak Y, Karakuş A. Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Univ Vet Fak Derg. June 2021;68(3):223-228. doi:10.33988/auvfd.727842
Chicago Padak, Yusuf, and Ayşe Karakuş. “Molecular Identification of Paramphistomidae Obtained from Ruminants in Van Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no. 3 (June 2021): 223-28. https://doi.org/10.33988/auvfd.727842.
EndNote Padak Y, Karakuş A (June 1, 2021) Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 3 223–228.
IEEE Y. Padak and A. Karakuş, “Molecular identification of Paramphistomidae obtained from ruminants in Van province”, Ankara Univ Vet Fak Derg, vol. 68, no. 3, pp. 223–228, 2021, doi: 10.33988/auvfd.727842.
ISNAD Padak, Yusuf - Karakuş, Ayşe. “Molecular Identification of Paramphistomidae Obtained from Ruminants in Van Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/3 (June 2021), 223-228. https://doi.org/10.33988/auvfd.727842.
JAMA Padak Y, Karakuş A. Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Univ Vet Fak Derg. 2021;68:223–228.
MLA Padak, Yusuf and Ayşe Karakuş. “Molecular Identification of Paramphistomidae Obtained from Ruminants in Van Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 68, no. 3, 2021, pp. 223-8, doi:10.33988/auvfd.727842.
Vancouver Padak Y, Karakuş A. Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Univ Vet Fak Derg. 2021;68(3):223-8.

Cited By

Morphological and molecular data confirm the occurrence of Paramphistomum leydeni (Trematoda: Paramphistomidae) in ruminants from Southern Brazil
Veterinary Parasitology: Regional Studies and Reports
https://doi.org/10.1016/j.vprsr.2024.101050
The winner takes it all: dominance ofCalicophoron daubneyi(Digenea: Paramphistomidae) among flukes in Central European beef cattle
Parasitology
https://doi.org/10.1017/S0031182021002158
Rumen Fluke in Cattle and Buffaloes in Asia: A Review
Pertanika Journal of Tropical Agricultural Science
https://doi.org/10.47836/pjtas.45.3.15