Research Article
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Year 2021, , 373 - 381, 27.09.2021
https://doi.org/10.33988/auvfd.793632

Abstract

References

  • Alvarez MV, Moreira MR, Ponce A (2012): Antiquorum Sensing and Antimicrobial Activity of Natural Agents with Potential Use in Food. J Food Saf, 32, 379-387.
  • Astani A, Zimmermann S, Hassan E, et al (2013): Antimicrobial activity of propolis special extract GH 2002 against multidrug-resistant clinical isolates. Die Pharmazie, 68, 695-701.
  • Banskota AH, Tezuka Y, Kadota S (2001): Recent progress in pharmacological research of propolis. Phytother Res, 15, 561-571.
  • Boonsai P, Phuwapraisirisan P, Chanchao C (2014): Antibacterial activity of a cardanol from Thai Apis mellifera propolis. Int J Med. Sci, 11, 327.
  • Bulman Z, Hudson A, Savka MA (2011). Propolis, a product of the bee hive, has an antagonistic affect on quorum-sensing regulated bioluminescence, gene transcription and motility. The FASEB Journal, 25, 948.
  • Bulman Z, Le P, Hudson AO, et al (2011): A novel property of propolis (bee glue): anti-pathogenic activity by inhibition of N-acyl-homoserine lactone mediated signaling in bacteria. J Ethnopharmacol, 138, 788-797.
  • De Castro S (2001): Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. Annu Rev Biomed, 3, 49-83.
  • De Marco S, Piccioni M, Pagiotti R, et al (2017): Antibiofilm and antioxidant activity of propolis and bud poplar resins versus Pseudomonas aeruginosa. eCAM, 2017.
  • Djais AA, Putri N, Jemmy J, et al (2019): Effect of Propolis on Streptococcus mutans Biofilm Formation. Pesqui Bras Odontopediatria Clin Integr, 19, 5221.
  • Dong Y-H, Zhang L-H (2005): Quorum sensing and quorum-quenching enzymes. J Microbiol , 43, 101-109.
  • Gemiarto AT, Ninyio NN, Lee SW, et al (2015): Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum. Anton Leeuw Int J G, 108, 491-504.
  • Gonsales G, Orsi R, Fernandes Júnior A, et al (2006): Antibacterial activity of propolis collected in different regions of Brazil. J Venom Anim Toxins Incl Trop Dis, 12, 276-284.
  • Kasote D, Ahmad A, Chen W, et al (2015): HPTLC-MS as an efficient hyphenated technique for the rapid identification of antimicrobial compounds from propolis. Phytochemistry Letters, 11, 326-331.
  • Koh KH, Tham F-Y (2011): Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. J Microbiol Immunol Infect, 44, 144-148.
  • Kubiliene L, Laugaliene V, Pavilonis A, et al (2015): Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complement Altern Med, 15, 156.
  • Lamberte LE, Cabrera EC, Rivera WL (2011): Activity of the Ethanolic Extract of Propolis (EEP) as a Potential Inhibitor of Quorum Sensing-Mediated Pigment Production in Chromobacterium violaceum and Virulence Factor Production in Pseudomonas aeruginosa. Philipp Agric Sci, 94, 14-22.
  • Lotfy M (2006): Biological activity of bee propolis in health and disease. Asian Pac J Cancer Prev, 7, 22-31.
  • Magaldi S, Mata-Essayag S, De Capriles CH, et al (2004): Well diffusion for antifungal susceptibility testing. Int J Infect Dis, 8, 39-45.
  • Majiene D, Trumbeckaite S, Pavilonis A, et al (2007): Antifungal and antibacterial activity of propolis. Curr Nutr Food Sci, 3, 304-308
  • McLean RJ, Pierson III LS, Fuqua C (2004): A simple screening protocol for the identification of quorum signal antagonists. J Microbiol Methods, 58, 351-360.
  • Noori A, Al-Ghamdi A, Ansari MJ, et al (2012): Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci, 9, 793.
  • Nyandwi R, Kılıç AS, Çelik M, et al (2019): Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi. East Afr J Sci, 1, 43-48.
  • Packiavathy IASV, Agilandeswari P, Musthafa KS, et al (2012): Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res Int, 45, 85-92.
  • Pobiega K, Kraśniewska K, Derewiaka D, et al (2019): Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods. J Food Sci Technol, 56, 5386-5395.
  • Przybyłek I, Karpiński TM (2019): Antibacterial properties of propolis. Molecules, 24, 2047.
  • Rasmussen TB, Givskov M (2006): Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol, 296, 149-161.
  • Santos HCD, Vieira DS, Yamamoto SM, et al (2019): Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis. Pesqui Vet Bras, 39, 954-960.
  • Sariyev R, Çayci M, Oruç HH (2019): Azerbaycan Propolislerinin Fenolik Madde İçerikleri ve Kalite Açısından Değerlendirilmesi. J Res Vet Med, 38, 1-6.
  • Savka MA, Dailey L, Popova M, et al (2015): Chemical composition and disruption of quorum sensing signaling in geographically diverse United States propolis. eCAM, 2015.
  • Sforcin JM, Bankova V (2011): Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133, 253-260.
  • Silva JC, Rodrigues S, Feás X, et al (2012): Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food ChemToxicol, 50, 1790-1795.
  • Song X, Xia Y-X, He Z-D, et al (2018): A review of natural products with anti-biofilm activity. Curr Org Chem, 22, 789-817.
  • Sorucu A, Oruç HH (2019): Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes. J Food Meas Charac, 13, 2461-2469.
  • Volpi N, Bergonzini G (2006): Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry. J Pharm Biomed Anal, 42, 354-361
  • Wayne P (2011): Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing.

Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis

Year 2021, , 373 - 381, 27.09.2021
https://doi.org/10.33988/auvfd.793632

Abstract

Quorum Sensing is a special system between bacteria to communicate with the signal molecules. Propolis is a resin collected from plants by honey bees. The present study examined anti-quorum sensing and antimicrobial activities of water and ethanol extracts of propolis. The antimicrobial activities of the propolis extracts were studied against Escherichia coli ATCC 25922 Gram (-), Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 Gram (+), Candida albicans ATCC 10239 (yeast) by the well diffusion method. Anti-quorum sensing activity, violacein inhibition activity, and anti-swarming activity were studied using Chromobacterium violaceum CV026 and C. violaceum ATCC 112472, and Pseudomonas aeruginosa (PA01) respectively. Both propolis extracts were detected antibacterial activity against Gram (+) bacteria and Candida albicans. However, antibacterial activity against Gram (-) bacteria were not observed. While the antimicrobial activity of water extract propolis was determined more effectively than ethanol extract, anti-quorum sensing and anti-swarming activities less effective. It was also detected that both extracts display violacein inhibition. It was revealed that the antimicrobial, anti-quorum sensing, anti-swarming activity depended on the dose of propolis extracts. Thirty-six phenolic compounds were analysed of propolis extract by HPLC-DAD. The amount and presence of phenolic compounds in the ethanol extract were more than the water extract apart from benzoic acid, cinnamic acid, syringic acid, (±) epigallocatechin, vitexin, rutin, and trans-chalcone. Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti- quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.

References

  • Alvarez MV, Moreira MR, Ponce A (2012): Antiquorum Sensing and Antimicrobial Activity of Natural Agents with Potential Use in Food. J Food Saf, 32, 379-387.
  • Astani A, Zimmermann S, Hassan E, et al (2013): Antimicrobial activity of propolis special extract GH 2002 against multidrug-resistant clinical isolates. Die Pharmazie, 68, 695-701.
  • Banskota AH, Tezuka Y, Kadota S (2001): Recent progress in pharmacological research of propolis. Phytother Res, 15, 561-571.
  • Boonsai P, Phuwapraisirisan P, Chanchao C (2014): Antibacterial activity of a cardanol from Thai Apis mellifera propolis. Int J Med. Sci, 11, 327.
  • Bulman Z, Hudson A, Savka MA (2011). Propolis, a product of the bee hive, has an antagonistic affect on quorum-sensing regulated bioluminescence, gene transcription and motility. The FASEB Journal, 25, 948.
  • Bulman Z, Le P, Hudson AO, et al (2011): A novel property of propolis (bee glue): anti-pathogenic activity by inhibition of N-acyl-homoserine lactone mediated signaling in bacteria. J Ethnopharmacol, 138, 788-797.
  • De Castro S (2001): Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. Annu Rev Biomed, 3, 49-83.
  • De Marco S, Piccioni M, Pagiotti R, et al (2017): Antibiofilm and antioxidant activity of propolis and bud poplar resins versus Pseudomonas aeruginosa. eCAM, 2017.
  • Djais AA, Putri N, Jemmy J, et al (2019): Effect of Propolis on Streptococcus mutans Biofilm Formation. Pesqui Bras Odontopediatria Clin Integr, 19, 5221.
  • Dong Y-H, Zhang L-H (2005): Quorum sensing and quorum-quenching enzymes. J Microbiol , 43, 101-109.
  • Gemiarto AT, Ninyio NN, Lee SW, et al (2015): Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum. Anton Leeuw Int J G, 108, 491-504.
  • Gonsales G, Orsi R, Fernandes Júnior A, et al (2006): Antibacterial activity of propolis collected in different regions of Brazil. J Venom Anim Toxins Incl Trop Dis, 12, 276-284.
  • Kasote D, Ahmad A, Chen W, et al (2015): HPTLC-MS as an efficient hyphenated technique for the rapid identification of antimicrobial compounds from propolis. Phytochemistry Letters, 11, 326-331.
  • Koh KH, Tham F-Y (2011): Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. J Microbiol Immunol Infect, 44, 144-148.
  • Kubiliene L, Laugaliene V, Pavilonis A, et al (2015): Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complement Altern Med, 15, 156.
  • Lamberte LE, Cabrera EC, Rivera WL (2011): Activity of the Ethanolic Extract of Propolis (EEP) as a Potential Inhibitor of Quorum Sensing-Mediated Pigment Production in Chromobacterium violaceum and Virulence Factor Production in Pseudomonas aeruginosa. Philipp Agric Sci, 94, 14-22.
  • Lotfy M (2006): Biological activity of bee propolis in health and disease. Asian Pac J Cancer Prev, 7, 22-31.
  • Magaldi S, Mata-Essayag S, De Capriles CH, et al (2004): Well diffusion for antifungal susceptibility testing. Int J Infect Dis, 8, 39-45.
  • Majiene D, Trumbeckaite S, Pavilonis A, et al (2007): Antifungal and antibacterial activity of propolis. Curr Nutr Food Sci, 3, 304-308
  • McLean RJ, Pierson III LS, Fuqua C (2004): A simple screening protocol for the identification of quorum signal antagonists. J Microbiol Methods, 58, 351-360.
  • Noori A, Al-Ghamdi A, Ansari MJ, et al (2012): Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci, 9, 793.
  • Nyandwi R, Kılıç AS, Çelik M, et al (2019): Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi. East Afr J Sci, 1, 43-48.
  • Packiavathy IASV, Agilandeswari P, Musthafa KS, et al (2012): Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res Int, 45, 85-92.
  • Pobiega K, Kraśniewska K, Derewiaka D, et al (2019): Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods. J Food Sci Technol, 56, 5386-5395.
  • Przybyłek I, Karpiński TM (2019): Antibacterial properties of propolis. Molecules, 24, 2047.
  • Rasmussen TB, Givskov M (2006): Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol, 296, 149-161.
  • Santos HCD, Vieira DS, Yamamoto SM, et al (2019): Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis. Pesqui Vet Bras, 39, 954-960.
  • Sariyev R, Çayci M, Oruç HH (2019): Azerbaycan Propolislerinin Fenolik Madde İçerikleri ve Kalite Açısından Değerlendirilmesi. J Res Vet Med, 38, 1-6.
  • Savka MA, Dailey L, Popova M, et al (2015): Chemical composition and disruption of quorum sensing signaling in geographically diverse United States propolis. eCAM, 2015.
  • Sforcin JM, Bankova V (2011): Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133, 253-260.
  • Silva JC, Rodrigues S, Feás X, et al (2012): Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food ChemToxicol, 50, 1790-1795.
  • Song X, Xia Y-X, He Z-D, et al (2018): A review of natural products with anti-biofilm activity. Curr Org Chem, 22, 789-817.
  • Sorucu A, Oruç HH (2019): Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes. J Food Meas Charac, 13, 2461-2469.
  • Volpi N, Bergonzini G (2006): Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry. J Pharm Biomed Anal, 42, 354-361
  • Wayne P (2011): Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing.
There are 35 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Ali Sorucu 0000-0002-0496-9498

Özgür Ceylan 0000-0002-1865-1093

Publication Date September 27, 2021
Published in Issue Year 2021

Cite

APA Sorucu, A., & Ceylan, Ö. (2021). Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(4), 373-381. https://doi.org/10.33988/auvfd.793632
AMA Sorucu A, Ceylan Ö. Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Univ Vet Fak Derg. September 2021;68(4):373-381. doi:10.33988/auvfd.793632
Chicago Sorucu, Ali, and Özgür Ceylan. “Determination of Antimicrobial and Anti-Quorum Sensing Activities of Water and Ethanol Extracts of Propolis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no. 4 (September 2021): 373-81. https://doi.org/10.33988/auvfd.793632.
EndNote Sorucu A, Ceylan Ö (September 1, 2021) Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 4 373–381.
IEEE A. Sorucu and Ö. Ceylan, “Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis”, Ankara Univ Vet Fak Derg, vol. 68, no. 4, pp. 373–381, 2021, doi: 10.33988/auvfd.793632.
ISNAD Sorucu, Ali - Ceylan, Özgür. “Determination of Antimicrobial and Anti-Quorum Sensing Activities of Water and Ethanol Extracts of Propolis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/4 (September 2021), 373-381. https://doi.org/10.33988/auvfd.793632.
JAMA Sorucu A, Ceylan Ö. Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Univ Vet Fak Derg. 2021;68:373–381.
MLA Sorucu, Ali and Özgür Ceylan. “Determination of Antimicrobial and Anti-Quorum Sensing Activities of Water and Ethanol Extracts of Propolis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 68, no. 4, 2021, pp. 373-81, doi:10.33988/auvfd.793632.
Vancouver Sorucu A, Ceylan Ö. Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Univ Vet Fak Derg. 2021;68(4):373-81.

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