Research Article
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Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice

Year 2023, , 21 - 28, 30.12.2022

Ceren Aştı , Hatice Öge

https://doi.org/10.33988/auvfd.877478

Abstract

Visceral Larvae Migrans (VLM) is a syndrome in humans, caused by Toxocara canis larvae. A current and completely successful treatment protocol against such a common infection has yet to be established. In this study, the effect of combination of albendazole and N. sativa oil for the treatment of VLM was investigated. Five experimental groups were constituted and a total of 125 Swiss albino (male, 6-8 weeks old) mice were used. All mice in each group were infected with 750 T. canis eggs with infective larvae, except the negative control group. 100 mg/kg albendazole and 0.15 ml Nigella sativa oil were applied orally to groups ALB and NSO separately and given orally to group COM in combination. The efficacy of the treatment was investigated parasitologically, histo-pathologically, and hematologically on the 7th, 14th, 28th, 45th, and 60th days post-infection with necropsies. The larval recovery analyses revealed that, the highest treatment efficacy was obtained in group of combination. The treatment efficacy was 72.46%, 48.81%, 36.25% in the groups of COM, ALB, and NSO, respectively. The most severe pathological changes were developed in Group ALB, and the inflammatory reactions and pathological changes in Groups of COM and NSO were mild. We conclude that N. sativa oil enhances the larvicidal effect of albendazole by having an anti-inflammatory effect and increasing tissue defense and immunity.

Keywords

Albendazole mice Nigella sativa Toxocara canis Visceral Larvae Migrans

Thanks

This study belongs to first author’s PhD thesis titled "Evaluation of Effect of Albendazol and Nigella Sativa Combination in Visceral Larvae Migrans (Toxocara canis) in Mice" that it has been done in Ankara University Faculty of Veterinary Medicine, Department of Parasitology. It was also presented as an oral presentation at III. International Ankara Conference on Scientific Research, 02-04.10.2020, Ankara, Türkiye. For their contribution in obtaining and interpreting the pathology findings of this study, we thank to Prof. Dr. Osman Kutsal and Assist. Prof. A. Selin Tunç.

References

  • Abukhader MM (2013): Thymoquinone in the clinical treatment of cancer: fact or fiction? Pharmacogn Revn, 7, 117-120.
  • Ahmad A, Husain A, Mujeeb M, et al (2013): A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed, 3, 337-352.
  • Avila LFC, Conceicao FR, Telmo PL, et al (2012): Saccharomyces boulardii reduces infection intensisty of mice with toxocariasis. Vet Parasitol, 187, 337-340.
  • Barrera MG, Leonardi D, Balmaro RE, et al (2010): In vivo evaluation of albendazole microspheres for the treatment of Toxocara canis larva migrans. Eur J Pharm Biopharm, 75, 451-454.
  • Cypess RH (1982): Visceral larvae migrans. 205-212. In: JH Steele (Ed), Handbook Series in Zoonoses, Section C: Parasitic Zoonoses. CRC Press, Florida.
  • El-Dakhakhny M, Madi NJ, Lembert N, et al (2002): Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats. J Ethnopharmacol, 81, 161-164.
  • El Gazzar MA (2007): Thymoquinone suppresses in vitro production of IL-5 and IL-13 by mast cells in reponse to lipopolysaccharide stimulation. Inflamm Res, 56, 345-351.
  • El-Shenawy NS, Soliman MFM, Eyad SI (2008): The effect of antioxidant properties of aqueous garlic extract and Nigella sativa as anti-schiostomiasis agents in mice. Rev Inst Med Trop S Paulo, 50, 29-36.
  • Fillaux J, Magnaval JF (2013): Laboratory diagnosis of human toxocariasis. Vet Parasitol, 193, 327-336.
  • Ghosheh OA, Houdi AA, Crooks PA (1999): High performance liquid chromatographic analysis pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L.). J Pharmaceut Biomed, 19, 757-762.
  • Gillespie SH (1988): The epidemiology of Toxocara canis. Parasitology Today, 4, 180-182.
  • Hajhashemi V, Ghannadi A, Jafarabadi H (2004): Black cumin seed essential oil, as a potent analgesic and antiinflammatory drug. Phytother Res, 18, 195-199.
  • Haq A, Abdullatif M, Lobo PI, et al (1995): Nigella sativa: effect on human lymphocytes and polymorphonuclear leukocyte phagocytic activity. Int Immunopharmacol, 30, 147-155.
  • Horiuchi A, Satou T, Akao N, et al (2005): The effect of free and polyethylene glycol-liposome-entrapped albendazole on larval mobility and number in Toxocara canis infected mice. Vet Parasitol, 129, 83-87.
  • Islam SKN, Begum P, Ahsan T, et al (2004): Immunosuppressive and cytotoxic properties of Nigella sativa. Phytother Res, 18, 395-398.
  • Kaya S (2007): Antelmentikler, homeopati ve tıbbi bitkiler. 489-545. In: S Kaya (Ed), Veteriner Farmakoloji. Medisan Yayınevi, Ankara.
  • Khan A, Khan MS, Avais M, et al (2007): Prevalence, hematology, and treatment of balantidiasis among donkeys in and around Lahore, Pakistan. Vet Parasitol, 196, 203-205.
  • Konuk T (1975): Pratik Fizyoloji I, Ankara Üniversitesi Yayınları, Ankara.
  • Leonardi D, Echenique C, Lamas C, et al (2009): High efficacy of albendazole -PEG 6000 in the treatment of Toxocara canis larva migrans infection. J Antimicrob Chemoth, 64, 375-378.
  • Liu W, Liu J, Yin D, et al (2015): Influence of ecological factors on the production of active substances in the anti-cancer plant Sinopodophyllum hexandrum (Royle) T.S. Ying. Plos One, 1-22.
  • Luna LE (1968): Manual of Histological Staining Methods of the Armed Forces Institue of Pathology. McGraw-Hill Book Company, New York.
  • Mady RF, El-Hadidy W, Elachy S (2016): Effect of Nigella sativa oil on experimental toxoplasmosis. Parasitol Res, 115, 379-390.
  • Magnaval JF, Glickman LT (2006): Management and treatment options for human toxocariasis. 113-126. In: CV Hollan, HV Smith (Eds), Toxocara Enigmatic Parasite. CABI Publishing, Oxfordshire.
  • Mahmood MS, Gilani AH, Khwaja A, et al (2003): The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production. Phytother Res, 17, 921-924.
  • Mahmoud MR, Al-Abnar HS, Saleh S (2002): The effect of Nigella sativa oil against the liver demage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol, 79, 1-11.
  • Majdalawieh AF, Fayyad MW (2015): Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: a comprehensive review. Int Immunopharmaco, 28, 295-304.
  • Majdalawieh AF, Hmaidan R, Carr RI (2010): Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol, 131, 268-275.
  • Mohany M, El-Feki M, Refaat I, et al (2012): Thymoquinone ameliorantes the immunological and histological changes induced by exposure to imidacloprid insecticed. J Toxicol Sci, 37, 1-11.
  • Moreira GMSG, Telmo PL, Mendonça M, et al (2014): Human toxocariasis: current advances in diagnostics, treatment and interventions. Trends Parasitol, 30, 456-464.
  • Musa D, Senocak G, Borazan G, et al (2011): Effects of Nigella sativa and albendazole alone and in combination in Toxocara canis infected mice. J Pak Med Assoc, 61, 866-870.
  • Othman AA (2012): Therapeutic battle against larval toxocariasis: are we still far behind?Acta Trop, 124,171-178.
  • Reis M, Trinca A, Ferreira MJU, et al (2010): Toxocara canis: potential activity of natural products against second-stage larvae in vitro and in vivo. Exp Parasitol, 126, 191-197.
  • Riviere JE (2009): Absorption, distribution, metabolism, and elimination. In: JE Riviere, MG Papich, HR Adams (Eds), Veterinary Pharmacology And Therapeutics. Wiley Blackwell, USA.
  • Rogerio AP, Sa-Nunes A, Albuquerque DA, et al (2003): Lafoensia pacari extract inhibitis IL-5 production in toxocariasis. Parasite Immunol, 25, 393-400.
  • Satou T, Horiuchi A, Akao N, et al (2005): Toxocara canis: search for a potential drug amongst ß-carboline alkolids- in vitro and mouse studies. Exp Parasitol, 110, 134-139.
  • Schnieder-Stock R, Fakhoury IH, Zaki AM, et al (2014): Thymoquinone: fifty years of success in the battle against cancer models. Drug Discov Today, 19, 18-30.
  • Tutun H, Sevin S, Yurdakök B, et al (2013): Bazı albendazol preparatlarının etken madde miktarının belirlenmesi. 174. In: IV. Ulusal Veteriner Farmakoloji Ve Toksikoloji Kongresi. Elazığ, Türkiye.
  • Woodhall DM, Fiore AR (2013): Toxocariasis: a review for pediatricians. J Ped Infect Dis, 3, 154-159.
  • Yarsan E, Altınsaat Ç, Aycicek H, et al (2003): Effects of albendazole treatment on haematological and biochemical parameters in healthy and Toxocara canis infected mice. Turk J Vet Anim Sci, 27, 1057-1063.

Year 2023, , 21 - 28, 30.12.2022

Ceren Aştı , Hatice Öge

https://doi.org/10.33988/auvfd.877478

Abstract

References

  • Abukhader MM (2013): Thymoquinone in the clinical treatment of cancer: fact or fiction? Pharmacogn Revn, 7, 117-120.
  • Ahmad A, Husain A, Mujeeb M, et al (2013): A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed, 3, 337-352.
  • Avila LFC, Conceicao FR, Telmo PL, et al (2012): Saccharomyces boulardii reduces infection intensisty of mice with toxocariasis. Vet Parasitol, 187, 337-340.
  • Barrera MG, Leonardi D, Balmaro RE, et al (2010): In vivo evaluation of albendazole microspheres for the treatment of Toxocara canis larva migrans. Eur J Pharm Biopharm, 75, 451-454.
  • Cypess RH (1982): Visceral larvae migrans. 205-212. In: JH Steele (Ed), Handbook Series in Zoonoses, Section C: Parasitic Zoonoses. CRC Press, Florida.
  • El-Dakhakhny M, Madi NJ, Lembert N, et al (2002): Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats. J Ethnopharmacol, 81, 161-164.
  • El Gazzar MA (2007): Thymoquinone suppresses in vitro production of IL-5 and IL-13 by mast cells in reponse to lipopolysaccharide stimulation. Inflamm Res, 56, 345-351.
  • El-Shenawy NS, Soliman MFM, Eyad SI (2008): The effect of antioxidant properties of aqueous garlic extract and Nigella sativa as anti-schiostomiasis agents in mice. Rev Inst Med Trop S Paulo, 50, 29-36.
  • Fillaux J, Magnaval JF (2013): Laboratory diagnosis of human toxocariasis. Vet Parasitol, 193, 327-336.
  • Ghosheh OA, Houdi AA, Crooks PA (1999): High performance liquid chromatographic analysis pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L.). J Pharmaceut Biomed, 19, 757-762.
  • Gillespie SH (1988): The epidemiology of Toxocara canis. Parasitology Today, 4, 180-182.
  • Hajhashemi V, Ghannadi A, Jafarabadi H (2004): Black cumin seed essential oil, as a potent analgesic and antiinflammatory drug. Phytother Res, 18, 195-199.
  • Haq A, Abdullatif M, Lobo PI, et al (1995): Nigella sativa: effect on human lymphocytes and polymorphonuclear leukocyte phagocytic activity. Int Immunopharmacol, 30, 147-155.
  • Horiuchi A, Satou T, Akao N, et al (2005): The effect of free and polyethylene glycol-liposome-entrapped albendazole on larval mobility and number in Toxocara canis infected mice. Vet Parasitol, 129, 83-87.
  • Islam SKN, Begum P, Ahsan T, et al (2004): Immunosuppressive and cytotoxic properties of Nigella sativa. Phytother Res, 18, 395-398.
  • Kaya S (2007): Antelmentikler, homeopati ve tıbbi bitkiler. 489-545. In: S Kaya (Ed), Veteriner Farmakoloji. Medisan Yayınevi, Ankara.
  • Khan A, Khan MS, Avais M, et al (2007): Prevalence, hematology, and treatment of balantidiasis among donkeys in and around Lahore, Pakistan. Vet Parasitol, 196, 203-205.
  • Konuk T (1975): Pratik Fizyoloji I, Ankara Üniversitesi Yayınları, Ankara.
  • Leonardi D, Echenique C, Lamas C, et al (2009): High efficacy of albendazole -PEG 6000 in the treatment of Toxocara canis larva migrans infection. J Antimicrob Chemoth, 64, 375-378.
  • Liu W, Liu J, Yin D, et al (2015): Influence of ecological factors on the production of active substances in the anti-cancer plant Sinopodophyllum hexandrum (Royle) T.S. Ying. Plos One, 1-22.
  • Luna LE (1968): Manual of Histological Staining Methods of the Armed Forces Institue of Pathology. McGraw-Hill Book Company, New York.
  • Mady RF, El-Hadidy W, Elachy S (2016): Effect of Nigella sativa oil on experimental toxoplasmosis. Parasitol Res, 115, 379-390.
  • Magnaval JF, Glickman LT (2006): Management and treatment options for human toxocariasis. 113-126. In: CV Hollan, HV Smith (Eds), Toxocara Enigmatic Parasite. CABI Publishing, Oxfordshire.
  • Mahmood MS, Gilani AH, Khwaja A, et al (2003): The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production. Phytother Res, 17, 921-924.
  • Mahmoud MR, Al-Abnar HS, Saleh S (2002): The effect of Nigella sativa oil against the liver demage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol, 79, 1-11.
  • Majdalawieh AF, Fayyad MW (2015): Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: a comprehensive review. Int Immunopharmaco, 28, 295-304.
  • Majdalawieh AF, Hmaidan R, Carr RI (2010): Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol, 131, 268-275.
  • Mohany M, El-Feki M, Refaat I, et al (2012): Thymoquinone ameliorantes the immunological and histological changes induced by exposure to imidacloprid insecticed. J Toxicol Sci, 37, 1-11.
  • Moreira GMSG, Telmo PL, Mendonça M, et al (2014): Human toxocariasis: current advances in diagnostics, treatment and interventions. Trends Parasitol, 30, 456-464.
  • Musa D, Senocak G, Borazan G, et al (2011): Effects of Nigella sativa and albendazole alone and in combination in Toxocara canis infected mice. J Pak Med Assoc, 61, 866-870.
  • Othman AA (2012): Therapeutic battle against larval toxocariasis: are we still far behind?Acta Trop, 124,171-178.
  • Reis M, Trinca A, Ferreira MJU, et al (2010): Toxocara canis: potential activity of natural products against second-stage larvae in vitro and in vivo. Exp Parasitol, 126, 191-197.
  • Riviere JE (2009): Absorption, distribution, metabolism, and elimination. In: JE Riviere, MG Papich, HR Adams (Eds), Veterinary Pharmacology And Therapeutics. Wiley Blackwell, USA.
  • Rogerio AP, Sa-Nunes A, Albuquerque DA, et al (2003): Lafoensia pacari extract inhibitis IL-5 production in toxocariasis. Parasite Immunol, 25, 393-400.
  • Satou T, Horiuchi A, Akao N, et al (2005): Toxocara canis: search for a potential drug amongst ß-carboline alkolids- in vitro and mouse studies. Exp Parasitol, 110, 134-139.
  • Schnieder-Stock R, Fakhoury IH, Zaki AM, et al (2014): Thymoquinone: fifty years of success in the battle against cancer models. Drug Discov Today, 19, 18-30.
  • Tutun H, Sevin S, Yurdakök B, et al (2013): Bazı albendazol preparatlarının etken madde miktarının belirlenmesi. 174. In: IV. Ulusal Veteriner Farmakoloji Ve Toksikoloji Kongresi. Elazığ, Türkiye.
  • Woodhall DM, Fiore AR (2013): Toxocariasis: a review for pediatricians. J Ped Infect Dis, 3, 154-159.
  • Yarsan E, Altınsaat Ç, Aycicek H, et al (2003): Effects of albendazole treatment on haematological and biochemical parameters in healthy and Toxocara canis infected mice. Turk J Vet Anim Sci, 27, 1057-1063.
There are 39 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Ceren Aştı 0000-0002-8424-2343

Hatice Öge 0000-0002-4799-3455

Publication Date December 30, 2022
Published in Issue Year 2023

Cite

APA Aştı, C., & Öge, H. (2022). Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 70(1), 21-28. https://doi.org/10.33988/auvfd.877478
AMA Aştı C, Öge H. Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice. Ankara Univ Vet Fak Derg. December 2022;70(1):21-28. doi:10.33988/auvfd.877478
Chicago Aştı, Ceren, and Hatice Öge. “Evaluation of the Effect of Albendazole and Nigella Sativa Combination on Visceral Larvae Migrans (Toxocara Canis) in Mice”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70, no. 1 (December 2022): 21-28. https://doi.org/10.33988/auvfd.877478.
EndNote Aştı C, Öge H (December 1, 2022) Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 1 21–28.
IEEE C. Aştı and H. Öge, “Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice”, Ankara Univ Vet Fak Derg, vol. 70, no. 1, pp. 21–28, 2022, doi: 10.33988/auvfd.877478.
ISNAD Aştı, Ceren - Öge, Hatice. “Evaluation of the Effect of Albendazole and Nigella Sativa Combination on Visceral Larvae Migrans (Toxocara Canis) in Mice”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70/1 (December 2022), 21-28. https://doi.org/10.33988/auvfd.877478.
JAMA Aştı C, Öge H. Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice. Ankara Univ Vet Fak Derg. 2022;70:21–28.
MLA Aştı, Ceren and Hatice Öge. “Evaluation of the Effect of Albendazole and Nigella Sativa Combination on Visceral Larvae Migrans (Toxocara Canis) in Mice”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 70, no. 1, 2022, pp. 21-28, doi:10.33988/auvfd.877478.
Vancouver Aştı C, Öge H. Evaluation of the effect of albendazole and Nigella sativa combination on Visceral Larvae Migrans (Toxocara canis) in mice. Ankara Univ Vet Fak Derg. 2022;70(1):21-8.