Research Article
Molecular diagnosis of neglected infectious agents of heep and attle abortions: the prevalences of Coxiella burnetii, Francisella tularensis and Chlamydophila abortus at a glance
Abstract
Bacterial abortive agents such as Brucella spp., Salmonella spp., Campylobacter spp., Listeria spp. cause serious infections that lead to significant economic losses in cattle and sheep breeding. These bacteria can be easily cultured under in vitro laboratory conditions. Abortions caused by intracellular bacteria such as Coxiella burnetii (C. burnetii), Chlamydophila abortus (C. abortus) and Francisella tularensis (F. tularensis) are less prevalent in abortive cases and the diagnosis of these bacteria, many of which need to cell culture for cultivation and biosafety level-3 laboratory facilities for safe working, can be made by PCR. In this study, it was aimed to determine the molecular prevalence of C. burnetii, C. abortus and F. tularensis agents, which were neglected during the diagnosis of abortions, in cattle and sheep collected from different regions of Türkiye. A total of 395 clinical materials were analyzed via agent-specific commercial Real-Time PCR. As a result, the molecular prevalence of F. tularensis, C. burnetii and C. abortus was determined as 14%, 2.9% and 2.28%, respectively. F. tularensis in cattle clinical samples and C. burnetii in sheep clinical samples were not found. This is the first report that F. tularensis was found as an agent in an abortive material in Türkiye. Although it differs in terms of prevalence, it has been determined that these microorganisms, which are neglected in routine diagnosis, can be spread with aborted materials, especially vaginal discharge, which may pose a risk of transmission.
Supporting Institution
This study which supported by the Ministry of Agriculture and Forestry (Türkiye).
References
- Akça D, Şahin M (2011): Investigation of Listeria species isolated from milk and vaginal swab samples of cows in the province of Kars, Turkey. Kafkas Univ Vet Fak Derg, 17, 987-993.
- Allam S, Hafez AA, Tolba S, et al (2017): Diagnosis of Chlamydophila abortus by species-specific ELISA technique with Real Time PCR confirmation in camels and small ruminants in the West-Northern Desert of Egypt. Int J Inov and App Res, 5, 17-25.
- Angelakis E, Raoult D (2010): Q Fever. Vet Microbiol, 140, 297-309.
- Aras Z, Sayın Z, Gölen SG (2017): Investigation of Chlamydophila abortus in abortion of cattle by PCR. Eurasian J Vet Sci, 33, 77-80.
- Bricker D (1931): Tularemia infection during pregnancy. Am J Nurs, 31, 979-982.
- Buse HY, Morris BJ, Rice EW (2020): Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR. BMC Microbiol, 20, 66.
- Büyük F, Özgen EK, Karakurt E, et al (2020): Accomplished management of Chlamydophila abortus-induced enzootic sheep Abortions: The case of Şavşat (Turkey). Kafkas Univ Vet Fak Derg, 26, 777-785.
- Cantas H, Muwonge A, Sareyyupoglu B, et al (2011): Q fever abortions in ruminants and associated on-farm risk factors in Northern Cyprus. BMC Vet Res, 7, 13.
- Cross AR, Baldwin VM, Roy S, et al (2019): Zoonoses under our noses. Microbes Infect, 21, 10-19.
- Dudley PB, Don CW (1936): Tularemia and pregnancy: Report of a case. J Am Med Assoc, 107, 577-578.
- Erdenliğ GS, Keskin O, Yiğin A, et al (2018): Investigation of Coxiella burnetii by Real-Time PCR in ruminant abortus cases. Harran Üniv Vet Fak Derg, 7, 79-83.
- Everett KDE (2000): Chlamydia and Chlamydiales: More than meets the eye. Vet Microbiol, 75, 109-126.
- Gojam A, Tulu D (2020): Infectious causes of abortion and its associated risk factor in sheep and goat in Ethiopia. Int J Vet Sci Technol, 4, 7-12.
- Guatteo R, Seegers H, Taurel AF, et al (2011): Prevalence of Coxiella burnetii infection in domestic ruminants: A critical review. Vet Microbiol, 149, 1-16.
- Gülmez Sağlam A, Şahin M (2016): Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey. Vet Med, 61, 17-22.
- İnci A, Doğanay M, Özdarendeli A, et al (2018): Overview of zoonotic diseases in Turkey: The one health concept and future threats. Turkiye Parazitol Derg, 42, 39-80.
- Junhui Z, Ruifu Y, Jianchun L, et al (1996): Detection of Francisella tularensis by the polymerase chain reaction. J Med Microbiol, 45, 477-482.
- Karataş Yeni D, Büyük F, Ashraf A, et al (2021): Tularemia: A re-emerging tick-borne infectious disease. Folia Microbiol (Praha), 66, 1-14.
- Karataş Yeni D, İzgür M (2015): Investigation on tularemia in potential reservoirs’ of Anatolia. Ankara Univ Vet Fak Derg, 62, 93-98.
- Karataş Yeni D, İzgür M (2015): Determination of the cross reaction of Tularemia titer and Brucella on sheep at specific disease focus in Turkey. Etlik Vet Mikrobiyol Derg, 26, 7-10.
- Kılıç A, Kalender H, Muz A (2010): The detection of Chlamydophila abortus from aborted bovine fetuses using PCR and microbiological culture. FÜ Sağ Bil Vet Derg, 24, 129-132.
- Kırkan Ş, Kaya O, Tekbıyık S, et al (2008): Detection of Coxiella burnetii in cattle by PCR. Turk J Vet Anim Sci, 32, 215-220.
- Knap N, Žele D, Glinšek Biškup U, et al (2019): The prevalence of Coxiella burnetii in ticks and animals in Slovenia. BMC Veterinary Research, 15, 368.
- Livingstone M, Wheelhouse N, Maley SW, et al (2009): Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing. Vet Microbiol, 135, 134-141.
- Longbottom D (2004): Chlamydial infections of domestic ruminants and swine: New nomenclature and new knowledge. Vet J, 168, 9-11.
- Maurin M, Raoult D (1999): Q fever. Clin Microbiol, 12, 518-553.
- O’Toole D, Williams ES, Woods LW, et al (2008): Tularemia in range sheep: An overlooked syndrome? J Vet Diagn Invest, 20, 508-513.
- Reichel MP, Wahl LC, Hill FI (2018): Review of diagnostic procedures and approaches to infectious causes of reproductive failures of cattle in Australia and New Zealand. Front Vet Sci, 5, 222.
- Schneeberger PM, Hermans MH, Van Hannen EJ (2010): Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever. Clin Vaccine Immunol, 17, 286-290.
- Selim A (2018): Molecular and serological data supporting the role of Q fever in abortions of sheep and 1 goats in northern. Microb Path, 125, 272-275.
- Sjostedt A, Eriksson U, Berglund L, et al (1997): Detection of Francisella tularensis in ulcers of patients with tularemia by PCR. J Clin Microbiol, 35, 1045-1048.
- Spickler AR (2017): Tularemia. Available at: http://www.cfsph.iastate.edu/DiseaseInfo/ factsheets.php. (Accessed March 16, 2019).
- Vidal S, Kegler K, Greub G, et al (2017): Neglected zoonotic agents in cattle abortion: Tackling the difficult to grow bacteria. BMC Vet Res, 13, 373.
- Weiss C (1943): Bacteriologic observations on infections of the eye. Arch Ophthalmol, 30, 110-137.
- Yilmaz GU, Gurcan Ş, Ozkan B, et al (2014): Investigation of the presence of Francisella tularensis by culture, serology and molecular methods in mice of Thrace Region, Turkey. Mikrobiyol Bul, 48, 213-222.
Year 2022,
, 425 - 430, 30.09.2022
Abstract
References
- Akça D, Şahin M (2011): Investigation of Listeria species isolated from milk and vaginal swab samples of cows in the province of Kars, Turkey. Kafkas Univ Vet Fak Derg, 17, 987-993.
- Allam S, Hafez AA, Tolba S, et al (2017): Diagnosis of Chlamydophila abortus by species-specific ELISA technique with Real Time PCR confirmation in camels and small ruminants in the West-Northern Desert of Egypt. Int J Inov and App Res, 5, 17-25.
- Angelakis E, Raoult D (2010): Q Fever. Vet Microbiol, 140, 297-309.
- Aras Z, Sayın Z, Gölen SG (2017): Investigation of Chlamydophila abortus in abortion of cattle by PCR. Eurasian J Vet Sci, 33, 77-80.
- Bricker D (1931): Tularemia infection during pregnancy. Am J Nurs, 31, 979-982.
- Buse HY, Morris BJ, Rice EW (2020): Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR. BMC Microbiol, 20, 66.
- Büyük F, Özgen EK, Karakurt E, et al (2020): Accomplished management of Chlamydophila abortus-induced enzootic sheep Abortions: The case of Şavşat (Turkey). Kafkas Univ Vet Fak Derg, 26, 777-785.
- Cantas H, Muwonge A, Sareyyupoglu B, et al (2011): Q fever abortions in ruminants and associated on-farm risk factors in Northern Cyprus. BMC Vet Res, 7, 13.
- Cross AR, Baldwin VM, Roy S, et al (2019): Zoonoses under our noses. Microbes Infect, 21, 10-19.
- Dudley PB, Don CW (1936): Tularemia and pregnancy: Report of a case. J Am Med Assoc, 107, 577-578.
- Erdenliğ GS, Keskin O, Yiğin A, et al (2018): Investigation of Coxiella burnetii by Real-Time PCR in ruminant abortus cases. Harran Üniv Vet Fak Derg, 7, 79-83.
- Everett KDE (2000): Chlamydia and Chlamydiales: More than meets the eye. Vet Microbiol, 75, 109-126.
- Gojam A, Tulu D (2020): Infectious causes of abortion and its associated risk factor in sheep and goat in Ethiopia. Int J Vet Sci Technol, 4, 7-12.
- Guatteo R, Seegers H, Taurel AF, et al (2011): Prevalence of Coxiella burnetii infection in domestic ruminants: A critical review. Vet Microbiol, 149, 1-16.
- Gülmez Sağlam A, Şahin M (2016): Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey. Vet Med, 61, 17-22.
- İnci A, Doğanay M, Özdarendeli A, et al (2018): Overview of zoonotic diseases in Turkey: The one health concept and future threats. Turkiye Parazitol Derg, 42, 39-80.
- Junhui Z, Ruifu Y, Jianchun L, et al (1996): Detection of Francisella tularensis by the polymerase chain reaction. J Med Microbiol, 45, 477-482.
- Karataş Yeni D, Büyük F, Ashraf A, et al (2021): Tularemia: A re-emerging tick-borne infectious disease. Folia Microbiol (Praha), 66, 1-14.
- Karataş Yeni D, İzgür M (2015): Investigation on tularemia in potential reservoirs’ of Anatolia. Ankara Univ Vet Fak Derg, 62, 93-98.
- Karataş Yeni D, İzgür M (2015): Determination of the cross reaction of Tularemia titer and Brucella on sheep at specific disease focus in Turkey. Etlik Vet Mikrobiyol Derg, 26, 7-10.
- Kılıç A, Kalender H, Muz A (2010): The detection of Chlamydophila abortus from aborted bovine fetuses using PCR and microbiological culture. FÜ Sağ Bil Vet Derg, 24, 129-132.
- Kırkan Ş, Kaya O, Tekbıyık S, et al (2008): Detection of Coxiella burnetii in cattle by PCR. Turk J Vet Anim Sci, 32, 215-220.
- Knap N, Žele D, Glinšek Biškup U, et al (2019): The prevalence of Coxiella burnetii in ticks and animals in Slovenia. BMC Veterinary Research, 15, 368.
- Livingstone M, Wheelhouse N, Maley SW, et al (2009): Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing. Vet Microbiol, 135, 134-141.
- Longbottom D (2004): Chlamydial infections of domestic ruminants and swine: New nomenclature and new knowledge. Vet J, 168, 9-11.
- Maurin M, Raoult D (1999): Q fever. Clin Microbiol, 12, 518-553.
- O’Toole D, Williams ES, Woods LW, et al (2008): Tularemia in range sheep: An overlooked syndrome? J Vet Diagn Invest, 20, 508-513.
- Reichel MP, Wahl LC, Hill FI (2018): Review of diagnostic procedures and approaches to infectious causes of reproductive failures of cattle in Australia and New Zealand. Front Vet Sci, 5, 222.
- Schneeberger PM, Hermans MH, Van Hannen EJ (2010): Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever. Clin Vaccine Immunol, 17, 286-290.
- Selim A (2018): Molecular and serological data supporting the role of Q fever in abortions of sheep and 1 goats in northern. Microb Path, 125, 272-275.
- Sjostedt A, Eriksson U, Berglund L, et al (1997): Detection of Francisella tularensis in ulcers of patients with tularemia by PCR. J Clin Microbiol, 35, 1045-1048.
- Spickler AR (2017): Tularemia. Available at: http://www.cfsph.iastate.edu/DiseaseInfo/ factsheets.php. (Accessed March 16, 2019).
- Vidal S, Kegler K, Greub G, et al (2017): Neglected zoonotic agents in cattle abortion: Tackling the difficult to grow bacteria. BMC Vet Res, 13, 373.
- Weiss C (1943): Bacteriologic observations on infections of the eye. Arch Ophthalmol, 30, 110-137.
- Yilmaz GU, Gurcan Ş, Ozkan B, et al (2014): Investigation of the presence of Francisella tularensis by culture, serology and molecular methods in mice of Thrace Region, Turkey. Mikrobiyol Bul, 48, 213-222.
There are 35 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 30, 2022 |
| Published in Issue | Year 2022 |