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The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

Year 2017, Volume: 64 Issue: 4, 289 - 299, 01.12.2017

Nilgün Antepli Feyzullah Beyaz

https://doi.org/10.1501/Vetfak_0000002812

Abstract

This study was aimed at demonstrating the immunohistochemical expression of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis of the rabbit and at investigating the potential role of these molecules in testicular and epididymal physiology. The testes and epididymis of 15 male adult New Zealand rabbits constituted the material of the study. The streptavidinbiotin complex (ABC) immunohistochemical technique was used to determine the localization of VEGF and its receptors flt-1 and flk1 in the testes and epididymis. In the testes, immunoreactivity for VEGF and its receptors flt-1 and flk-1 was observed in the Sertoli cells and spermatogonia in the seminiferous tubules throughout all of the spermatogenic stages from I to VIII and in the Leydig cells of the interstitium. Furthermore, immunoreactivity was also observed in the acrosome of the spermatids in seminiferous tubules for VEGF at stages V, VI, VII and VIII and in the acrosome of the spermatids at stages VII and VIII and the blood vessels of the interstitium for flk-1. Positive immunoreactivity, ranging from strong to weak, was detected for VEGF, flt-1 and flk-1 in the cytoplasm of the principal, apical, basal and clear cells of the epithelium lining the epididymal initial segment and the caput, corpus and cauda epididymidis. The specific localization of the VEGF protein and its receptors flt-1 and flk-1 in the testes of the rabbit suggests that these molecules could have a functional role in several processes, including spermatogonial differentiation, spermiogenesis, acrosome development in spermatids, steroidogenesis in Leydig cells, epididymal sperm maturation, and the maintenance of the bloodepididymis barrier

Keywords

Epididymis rabbit testis VEGF VEGF receptors

References

  • Ai QY, Tian H, Zhag J, et al. (2008): Expression of VEGF and Flt-1 in the testis, epididymis and epididymal sperm of adolescent rats. Zhonghua Nan Ke Xue, 14, 871-875.
  • Bates DO, Hillman NJ, Williams B, et al. (2002): Regulation of microvascular permeability by vascular endothelial growth factors. J Anat, 200, 581-597.
  • Bott RC, Clopton DT, Cupp AS (2008): A proposed role for VEGF isoforms in sex-specific vasculature development in the gonad. Reprod Domestic Anim, 43, 310-316.
  • Breen EC (2007): VEGF in biological control. J Cellular Biochem, 102, 1358-1367.
  • Cornwall GA (2009): New insight into epididymal biology and function. Human Reprod Update, 15, 213-227.
  • Çakır A, Bozkurt EÜ, Düzler A (2001): Tavşan, kobay ve sıçanda testisin şekli, konumu ve testis epididymis ilişkisi. Ankara Üniv Vet Fak Derg, 48, 129-132.
  • Ergün A, Kiliç N, Fiedler W, et al. (1997): Vascular endothelial growth factor and its receptors in normal human testicular tissue. Mol Cell Endocrinol, 131, 9-20.
  • Ergün S, Luttmer W, Fiedler W, et al. (1998): Functional expression and localization of vascular endothelial growth factor and its receptors in the human epididymis. Biol Reprod, 58, 160-168.
  • Ferrara N, Gerber HP, Le Couter J (2003): The biology of VEGF and its receptors. Nat Med, 9, 669-676.
  • Maharaj ASR, D’Amore PA (2007): Roles for VEGF in the adult. Microvascular Res, 74, 100-113.
  • Maharaj ASR, Saint-Geniez, Maldonado AE, et al. (2006): Vascular endothelial growth factor localization in the adult. Am J Pathol, 168, 639-648.
  • Nalbandian A, Dettin L, Dym M, et al. (2003): Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse. Biol Reprod, 69, 985- 994.
  • Nieminen T, Toivanen PI, Rintanen N, et al. (2004): The impact of the receptors binding profiles of the vascular endothelial growth factors on their angiogenic features. Biochem Biophysica Acta, 1840, 454-463.
  • Obermair A, Obruca A, Pöhl M, et al. (1999): Vascular endothelial growth factor and its receptors in male fertility. Fertil Steril, 72, 269-275.
  • Otrock ZK, Makarem JA, Shamseddine AI (2007): Vascular endothelial growth factor family of ligands and receptors: Review. Blood Cell Mol Dis, 38, 258-268.
  • Petrova TV, Makinen T, Alitalo K (1999): Signalling via vascular endothelial growth factor receptors. Exp Cell Res, 253,117-130.
  • Reddy N, Kasukurthi KB, Mahla RS, et al. (2012): Expression of vascular endothelial growth factor (VEGF) transcript and protein in the testis of several vertebrates, including endangered species. Theriogenology, 77, 608- 614.
  • Roy H, Bhardwaj S, Yla-Herttuala H (2006): Biology of vascular endothelial growth factors. FEBS Letters, 2879- 2887.
  • Schön J, Blottner S, Gabler C, et al. (2010): Vascular endothelial growth factor A is a putative paracrine regulator in seasonally controlled spermatogenesis: Insights from a ruminant model, the roe deer. Growth Factors, 28, 202-210.
  • Swierstra EE, Foote RH (1963): Cytology and kinetics of spermatogenesis in the rabbit. J Reprod Fertil, 5, 309-322.
  • Qui Y, Hoareau-Aveilla C, Oltean S, et al. (2009): The angiogenic isoforms of VEGF in health and disease. Biochem Soc Trans, 37, 1207-1213.
  • Veikkola T, Alitalo K (1999): VEGFs, receptors and angiogenesis. Semin Cancer Biol, 9, 211-220.
  • Zhang QY, Qui SD, Ge L (2004): Studies on expression and location of VEGF protein in rat testis and epididymis. Shi Yan Sheng Wu Xue Bao, 37, 1-8.

Tavşan testis ve epididimislerinde vasküler endoteliyal büyüme faktörü ve reseptörlerininimmunohistokimyasal lokalizasyonu

Year 2017, Volume: 64 Issue: 4, 289 - 299, 01.12.2017

Nilgün Antepli Feyzullah Beyaz

https://doi.org/10.1501/Vetfak_0000002812

Abstract

Bu çalışmanın amacı, VEGF ve reseptörleri flt-1 ile flk-1’in tavşan testis ve epididimisindeki immunohistokimyasal ekspresyonlarını belirlemek ve bu moleküllerin testis ve epididimis fizyolojisindeki potansiyel rollerini araştırmaktır. Çalışmada materyal olarak, 15 adet sağlıklı, erkek Yeni Zellanda tavşanından elde edilen testis ve epididimisler kullanıldı. VEGF ve reseptörleri flt-1 ile flk-1’in testis ve epididimisdeki lokalizasyonlarının belirlenmesi amacıyla streptavidin biotin kompleks (ABC) immunohistokimyasal yöntemi kullanıldı. Testiste, I’den VIII’e kadarki tüm spermatojenik evrelerde seminifer tubüllerdeki Sertoli hücreleri ve spermatogonialarda ve intersitisyumda Leydig hücrelerinde VEGF ve reseptörleri flt-1 ile flk-1 için immunreaktivite gözlendi. Buna ilaveten, seminifer tubüllerde V., VI., VII. ve VIII. evrelerde VEGF için, VII. ve VIII. spermatojenik evrelerde ise flk1 için spermatidlerin akrozomlarında ve intersitisyumda kan damarlarında immunreaktivite belirlendi. Epididimiste, başlangıç segmenti, kaput, korpus ve kauda epididimidis epitelindeki prensipal, apikal ve bazal hücreleri ile clear hücrelerinin sitoplazmalarında zayıftan şiddetliye değişen derecelerde VEGF, flt-1 ve flk-1 için pozitif immunreaktivite tespit edildi. VEGF proteini ve reseptörleri flt-1 ve flk-1’in tavşan testislerindeki spesifik lokalizasyonları, bu moleküllerin spermatogonial farklılaşma, spermiyogenezis, spermatidlerde akrozom gelişimi ve Leydig hücrelerinde steroidiogeneziste, ayrıca epididimiste sperm olgunlaşması ve kan-epididimis bariyerine katkı sağlama gibi fonksiyonel görevleri olabileceğini göstermektedir

Keywords

Epididimis tavşan testis VEGF VEGF reseptörleri

References

  • Ai QY, Tian H, Zhag J, et al. (2008): Expression of VEGF and Flt-1 in the testis, epididymis and epididymal sperm of adolescent rats. Zhonghua Nan Ke Xue, 14, 871-875.
  • Bates DO, Hillman NJ, Williams B, et al. (2002): Regulation of microvascular permeability by vascular endothelial growth factors. J Anat, 200, 581-597.
  • Bott RC, Clopton DT, Cupp AS (2008): A proposed role for VEGF isoforms in sex-specific vasculature development in the gonad. Reprod Domestic Anim, 43, 310-316.
  • Breen EC (2007): VEGF in biological control. J Cellular Biochem, 102, 1358-1367.
  • Cornwall GA (2009): New insight into epididymal biology and function. Human Reprod Update, 15, 213-227.
  • Çakır A, Bozkurt EÜ, Düzler A (2001): Tavşan, kobay ve sıçanda testisin şekli, konumu ve testis epididymis ilişkisi. Ankara Üniv Vet Fak Derg, 48, 129-132.
  • Ergün A, Kiliç N, Fiedler W, et al. (1997): Vascular endothelial growth factor and its receptors in normal human testicular tissue. Mol Cell Endocrinol, 131, 9-20.
  • Ergün S, Luttmer W, Fiedler W, et al. (1998): Functional expression and localization of vascular endothelial growth factor and its receptors in the human epididymis. Biol Reprod, 58, 160-168.
  • Ferrara N, Gerber HP, Le Couter J (2003): The biology of VEGF and its receptors. Nat Med, 9, 669-676.
  • Maharaj ASR, D’Amore PA (2007): Roles for VEGF in the adult. Microvascular Res, 74, 100-113.
  • Maharaj ASR, Saint-Geniez, Maldonado AE, et al. (2006): Vascular endothelial growth factor localization in the adult. Am J Pathol, 168, 639-648.
  • Nalbandian A, Dettin L, Dym M, et al. (2003): Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse. Biol Reprod, 69, 985- 994.
  • Nieminen T, Toivanen PI, Rintanen N, et al. (2004): The impact of the receptors binding profiles of the vascular endothelial growth factors on their angiogenic features. Biochem Biophysica Acta, 1840, 454-463.
  • Obermair A, Obruca A, Pöhl M, et al. (1999): Vascular endothelial growth factor and its receptors in male fertility. Fertil Steril, 72, 269-275.
  • Otrock ZK, Makarem JA, Shamseddine AI (2007): Vascular endothelial growth factor family of ligands and receptors: Review. Blood Cell Mol Dis, 38, 258-268.
  • Petrova TV, Makinen T, Alitalo K (1999): Signalling via vascular endothelial growth factor receptors. Exp Cell Res, 253,117-130.
  • Reddy N, Kasukurthi KB, Mahla RS, et al. (2012): Expression of vascular endothelial growth factor (VEGF) transcript and protein in the testis of several vertebrates, including endangered species. Theriogenology, 77, 608- 614.
  • Roy H, Bhardwaj S, Yla-Herttuala H (2006): Biology of vascular endothelial growth factors. FEBS Letters, 2879- 2887.
  • Schön J, Blottner S, Gabler C, et al. (2010): Vascular endothelial growth factor A is a putative paracrine regulator in seasonally controlled spermatogenesis: Insights from a ruminant model, the roe deer. Growth Factors, 28, 202-210.
  • Swierstra EE, Foote RH (1963): Cytology and kinetics of spermatogenesis in the rabbit. J Reprod Fertil, 5, 309-322.
  • Qui Y, Hoareau-Aveilla C, Oltean S, et al. (2009): The angiogenic isoforms of VEGF in health and disease. Biochem Soc Trans, 37, 1207-1213.
  • Veikkola T, Alitalo K (1999): VEGFs, receptors and angiogenesis. Semin Cancer Biol, 9, 211-220.
  • Zhang QY, Qui SD, Ge L (2004): Studies on expression and location of VEGF protein in rat testis and epididymis. Shi Yan Sheng Wu Xue Bao, 37, 1-8.
There are 23 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA67AU36BH
Journal Section Research Article
Authors

Nilgün Antepli

Feyzullah Beyaz

Publication Date December 1, 2017
Published in Issue Year 2017Volume: 64 Issue: 4

Cite

APA Antepli, N., & Beyaz, F. (2017). The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(4), 289-299. https://doi.org/10.1501/Vetfak_0000002812
AMA Antepli N, Beyaz F. The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit. Ankara Univ Vet Fak Derg. December 2017;64(4):289-299. doi:10.1501/Vetfak_0000002812
Chicago Antepli, Nilgün, and Feyzullah Beyaz. “The Immunohistochemical Localization of the Vascular Endothelial Growth Factor and Its Receptors in the Testes and Epididymis of the Rabbit”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64, no. 4 (December 2017): 289-99. https://doi.org/10.1501/Vetfak_0000002812.
EndNote Antepli N, Beyaz F (December 1, 2017) The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 4 289–299.
IEEE N. Antepli and F. Beyaz, “The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit”, Ankara Univ Vet Fak Derg, vol. 64, no. 4, pp. 289–299, 2017, doi: 10.1501/Vetfak_0000002812.
ISNAD Antepli, Nilgün - Beyaz, Feyzullah. “The Immunohistochemical Localization of the Vascular Endothelial Growth Factor and Its Receptors in the Testes and Epididymis of the Rabbit”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64/4 (December 2017), 289-299. https://doi.org/10.1501/Vetfak_0000002812.
JAMA Antepli N, Beyaz F. The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit. Ankara Univ Vet Fak Derg. 2017;64:289–299.
MLA Antepli, Nilgün and Feyzullah Beyaz. “The Immunohistochemical Localization of the Vascular Endothelial Growth Factor and Its Receptors in the Testes and Epididymis of the Rabbit”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 64, no. 4, 2017, pp. 289-9, doi:10.1501/Vetfak_0000002812.
Vancouver Antepli N, Beyaz F. The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit. Ankara Univ Vet Fak Derg. 2017;64(4):289-9.