Letter to Editor
Characterization of extended-spectrum beta-lactamase-producing fecal Escherichia coli isolates in laying hens
Abstract
The purposes of the present study were to determine whether healthy laying hens in Burdur province of Turkey carried extended-spectrum beta-lactamase (ESBL)- producing Escherichia coli in their intestinal flora and to further characterize the E. coli isolates and ESBL genes. A total of 200 fecal samples from three laying hen farms were cultured in Brilliance E. coli / coliform Selective Agar supplemented with cefotaxime or ceftazidime. ESBL-producing E. coli isolates determined by phenotypic tests were screened by Polymerase Chain Reaction (PCR) for ESBL genes, and ESBL genes were sequenced. Phylogenetic analysis of the E. coli isolates were performed by PCR. ESBL-producing E. coli were isolated from 12 (6.0%) laying hen fecal samples by phenotypic tests. According to PCR screening and sequence analysis, blaCTX-M group 1 (blaCTX-M-3 or blaCTX-M-15) genes were detected in the E. coli isolates. Phylogenetic analysis of the E. coli isolates showed that group D was the predominant group (58.3%) and none of the isolates belonged to group B2. Consequently, present study reports the first characterisation of CTX-M-3 and CTX-M-15 type ESBL-producing E. coli isolates on laying hen production from Turkey
References
- Arpin C, Dubois V, Coulange L, et al. (2003): Extended- spectrum beta-lactamase-producing Enterobacteriaceae in community and private health care centers. Antimicrob Agents Chemother, 47, 3506-3514.
- Azap OK, Arslan H, Serefhanoğlu K, et al. (2010): Risk factors for extended-spectrum beta-lactamase positivity in uropathogenic Escherichia coli isolated from community- acquired urinary tract infections. Clin Microbiol Infect, 16,147-151.
- Basaran Kahraman B, Diren Siğirci B, Celik B, et al. (2016): Detection of extended-spectrum beta-lactamase and AmpC beta-lactamase producing Escherichia coli isolates from chickens. Kafkas Univ Vet Fak Derg, 22, 591-596.
- Bortolaia V, Guardabassi L, Bisgaard M (2010): Escherichia coli producing CTX-M-1, -2, and -9 group beta-lactamases in organic chicken egg production. Antimicrob Agents Chemother, 54, 3527-3528.
- Clermont O, Bonacorsi S, Bingen E (2000): Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol, 66, 4555-4558.
- Clinical and Laboratory Standards Institute (CLSI) (2010): Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 3rd Ed. Approved standards M31-A3. Wayne, PA, USA.
- Clinical and Laboratory Standards Institute (CLSI) (2013): Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 2nd Informational supplement VET01-S2. Wayne, PA, USA.
- Clinical and Laboratory Standards Institute (CLSI) (2015): susceptibility testing. 24th Informational supplement M100- S25. Wayne, PA, USA. for antimicrobial
- Costa D, Poeta P, Saenz Y, et al. (2006): Detection of Escherichia coli harbouring extended-spectrum beta- lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal. J Antimicrob Chemother, 58, 1311-1312.
- Escobar-Páramo P, Le Menac'h A, Le Gall T, et al. (2006): Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Environ Microbiol, 8, 1975-1984.
- Frere JM (1995): Beta-lactamases and bacterial resistance to antibiotics. Mol Microbiol, 16, 385-395.
- Gonullu N, Aktas Z, Kayacan CB, et al. (2008): Dissemination of CTX-M-15 beta-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a University hospital in Istanbul, Turkey. J Clin Microbiol, 46, 1110-1112.
- Heffernan HM, Woodhouse RE, Pope CE, et al. (2009): Prevalence and types of extended-spectrum beta- lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand. Int J Antimicrob Agents, 34, 544-549.
- Higgins J, Hohn C, Hornor S, et al. (2007): Genotyping of Escherichia coli from environmental and animal samples. J Microbiol Methods, 70, 227-235.
- Jeong SH, Bae IK, Kwon SB, et al. (2005): Dissemination of transferable CTX-M-type extended-spectrum beta- lactamase-producing Escherichia coli in Korea. J Appl Microbiol, 98, 921-927.
- Kilani H, Abbassi MS,Ferjani S, et al. (2015): Occurrence of blaCTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. Front Cell Infect Microbiol, 5, 38.
- Li L, Jiang ZG, Xia LN, et al. (2010): Characterization of antimicrobial resistance and molecular determinants of beta-lactamase in Escherichia coli isolated from chickens in China during 1970-2007. Vet Microbiol, 144, 505-510.
- Livermore DM (2012): Current epidemiology and growing resistance of gram-negative pathogens. Korean J Intern Med, 27, 128-142.
- Pitout JD, Laupland KB (2008): Extended-spectrum beta- lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis, 8, 159-166.
- Rossolini GM, D’Andrea MM, Mugnaioli C (2008): The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect, 14 (Suppl. 1), 33-41.
- Saladin M, Cao VT, Lambert T, et al. (2002): Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett, 209, 161-168.
- Tenover FC, Rasheed JK (2004): Detection of antimicrobial resistance genes and mutations associated with antimicrobial resistance in microorganisms. 391-406. In: DH Persing, FC Tenover, J Versalovic, et al. (Ed), Molecular Microbiology: Diagnostic Principles and Practice. ASM Publishing, Washington DC, USA.
- Ünal N, Dilik Z, Aşkar Ş, et al. (2014): Prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli isolates in fecal samples of broilers. Proceedings of the 11th National Congress of Veterinary Microbiology. Antalya, Turkey.
- Wang G, Clark CG, Rodgers FG (2002): Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 shiga toxin family by multiplex PCR. J Clin Microbiol, 40, 3613-3619.
- Wasyl D, Hasman H, Cavaco LM, et al. (2012): Prevalence and characterization of cephalosporin resistance in nonpathogenic Escherichia coli from food-producing animals slaughtered in Poland. Microb Drug Resist, 18, 79-82.
- Winn W, Allen S, Janda W, et al. (2006): Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Lippincott Williams and Wilkins, Philadelphia, PA, USA.
Yumurtacı tavuklarda genişlemiş spektrumlu beta laktamaz üreten dışkı kökenli Escherichia coliizolatlarının karakterizasyonu
Abstract
Bu çalışmada, Burdur ilindeki yumurtacı tavukların bağırsak mikroflorasında genişlemiş spektrumlu beta laktamaz (GSBL) üreten Escherichia coli varlığının ortaya çıkarılması ve izole edilen E. coli izolatlarının ve taşıdıkları GSBL genlerinin karakterize edilmesi amaçlandı. Toplam üç tavuk çiftliğinden alınan 200 dışkı örneği, içerisine sefotaksim ve seftazidim ilave edilmiş Brilliance E. coli / koliform Selektif Agara ekildi. Fenotipik testlerle belirlenen GSBL üreten E. coli izolatları GSBL genleri yönünden Polimeraz Zincir Reaksiyonu (PZR) ile tarandı ve GSBL genlerinin sekans analizi yapıldı. İzolatların filogenetik analizi PZR ile gerçekleştirildi. Fenotipik testler ile GSBL üreten E. coli 12 (%6.0) adet dışkı örneğinde belirlendi. PZR ve sekans analizine göre, E. coli izolatlarında blaCTX-M grup 1 genleri (blaCTX-M-3 ve blaCTX-M-15) belirlendi. İzolatların filogenetik analizi grup D’nin en yaygın olduğunu (%58.3) ve izolatların hiç birinin B2 grubundan olmadığını gösterdi. Sonuç olarak, bu çalışma Türkiye’de yumurta tavuklarında CTX-M-3 and CTX-M-15 type GSBL üreten E. coli izolatlarını tanımlayan ilk rapordur
References
- Arpin C, Dubois V, Coulange L, et al. (2003): Extended- spectrum beta-lactamase-producing Enterobacteriaceae in community and private health care centers. Antimicrob Agents Chemother, 47, 3506-3514.
- Azap OK, Arslan H, Serefhanoğlu K, et al. (2010): Risk factors for extended-spectrum beta-lactamase positivity in uropathogenic Escherichia coli isolated from community- acquired urinary tract infections. Clin Microbiol Infect, 16,147-151.
- Basaran Kahraman B, Diren Siğirci B, Celik B, et al. (2016): Detection of extended-spectrum beta-lactamase and AmpC beta-lactamase producing Escherichia coli isolates from chickens. Kafkas Univ Vet Fak Derg, 22, 591-596.
- Bortolaia V, Guardabassi L, Bisgaard M (2010): Escherichia coli producing CTX-M-1, -2, and -9 group beta-lactamases in organic chicken egg production. Antimicrob Agents Chemother, 54, 3527-3528.
- Clermont O, Bonacorsi S, Bingen E (2000): Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol, 66, 4555-4558.
- Clinical and Laboratory Standards Institute (CLSI) (2010): Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 3rd Ed. Approved standards M31-A3. Wayne, PA, USA.
- Clinical and Laboratory Standards Institute (CLSI) (2013): Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 2nd Informational supplement VET01-S2. Wayne, PA, USA.
- Clinical and Laboratory Standards Institute (CLSI) (2015): susceptibility testing. 24th Informational supplement M100- S25. Wayne, PA, USA. for antimicrobial
- Costa D, Poeta P, Saenz Y, et al. (2006): Detection of Escherichia coli harbouring extended-spectrum beta- lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal. J Antimicrob Chemother, 58, 1311-1312.
- Escobar-Páramo P, Le Menac'h A, Le Gall T, et al. (2006): Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Environ Microbiol, 8, 1975-1984.
- Frere JM (1995): Beta-lactamases and bacterial resistance to antibiotics. Mol Microbiol, 16, 385-395.
- Gonullu N, Aktas Z, Kayacan CB, et al. (2008): Dissemination of CTX-M-15 beta-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a University hospital in Istanbul, Turkey. J Clin Microbiol, 46, 1110-1112.
- Heffernan HM, Woodhouse RE, Pope CE, et al. (2009): Prevalence and types of extended-spectrum beta- lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand. Int J Antimicrob Agents, 34, 544-549.
- Higgins J, Hohn C, Hornor S, et al. (2007): Genotyping of Escherichia coli from environmental and animal samples. J Microbiol Methods, 70, 227-235.
- Jeong SH, Bae IK, Kwon SB, et al. (2005): Dissemination of transferable CTX-M-type extended-spectrum beta- lactamase-producing Escherichia coli in Korea. J Appl Microbiol, 98, 921-927.
- Kilani H, Abbassi MS,Ferjani S, et al. (2015): Occurrence of blaCTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. Front Cell Infect Microbiol, 5, 38.
- Li L, Jiang ZG, Xia LN, et al. (2010): Characterization of antimicrobial resistance and molecular determinants of beta-lactamase in Escherichia coli isolated from chickens in China during 1970-2007. Vet Microbiol, 144, 505-510.
- Livermore DM (2012): Current epidemiology and growing resistance of gram-negative pathogens. Korean J Intern Med, 27, 128-142.
- Pitout JD, Laupland KB (2008): Extended-spectrum beta- lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis, 8, 159-166.
- Rossolini GM, D’Andrea MM, Mugnaioli C (2008): The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect, 14 (Suppl. 1), 33-41.
- Saladin M, Cao VT, Lambert T, et al. (2002): Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett, 209, 161-168.
- Tenover FC, Rasheed JK (2004): Detection of antimicrobial resistance genes and mutations associated with antimicrobial resistance in microorganisms. 391-406. In: DH Persing, FC Tenover, J Versalovic, et al. (Ed), Molecular Microbiology: Diagnostic Principles and Practice. ASM Publishing, Washington DC, USA.
- Ünal N, Dilik Z, Aşkar Ş, et al. (2014): Prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli isolates in fecal samples of broilers. Proceedings of the 11th National Congress of Veterinary Microbiology. Antalya, Turkey.
- Wang G, Clark CG, Rodgers FG (2002): Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 shiga toxin family by multiplex PCR. J Clin Microbiol, 40, 3613-3619.
- Wasyl D, Hasman H, Cavaco LM, et al. (2012): Prevalence and characterization of cephalosporin resistance in nonpathogenic Escherichia coli from food-producing animals slaughtered in Poland. Microb Drug Resist, 18, 79-82.
- Winn W, Allen S, Janda W, et al. (2006): Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Lippincott Williams and Wilkins, Philadelphia, PA, USA.
There are 26 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA57YF54KT |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2017 |
| Published in Issue | Year 2017Volume: 64 Issue: 4 |