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Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Year 2017, Volume: 64 Issue: 4, 337 - 343, 01.12.2017

Davut Bayram Korhan Arslan Bilal Akyüz Kaan Muhsin İşcan

https://doi.org/10.1501/Vetfak_0000002818
Cited By: 1

Abstract

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X2:40.75, p<0.001and X2:8.4, p<0.01)

Keywords

Holstein leptin pit-I polymorphism RFLP

References

  • Almeida S, Almeida E, Moraes J, et al. (2003): Molecular markers in the LEP gene and reproductive performance of beef cattle. J Anim Breed Genet, 120, 106-113
  • Aytekin I, Boztepe S (2013): Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J Anim Plant Sci, 23, 1281-1289.
  • Block S, Butler W, Bell A, et al. (2001): Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol, 171, 339-348.
  • Buchanan F, Van Kessel A, Waldner C, et al. (2003): Hot topic: An association between a leptin single nucleotide polymorphism and milk and protein yield. J Dairy Sci, 86, 3164-3166.
  • Clempson AM, Pollott GE, Brickell JS, et al. (2011): Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. J Dairy Sci, 94, 3618-3628.
  • Cohen LE, Wondisford FE, Radovick S (1997): Role of Pit-I in the gene expression of growth hormone, prolactin, and thyrotropin. Endocrinol Metab Clin N Am, 25, 23-540.
  • Cosier V, Vlaic A, Gaboreanu I (2007): HinfI polymorphism of k-casein and Pit1 genes in Romanian Simmental cattle. Sci P Anim Sci Bio, 40, 59-64.
  • Dario C, Selvaggi M, Carnicella D, et al. (2009): Bufano G: STAT5A/Aval polymorphism in Podolica bulls and its effect on growth performance traits. Livest Sci, 123, 83-87.
  • Doosti A, Arshi A, Bomeni B (2011): Molecular study of PIT1 gene polymorphism in Holstein and Iranian native cattle. Ajar, 6, 4467-4470.
  • Dybus A, Szatkowska I, Czerniawska-Piatkowska E, et al. (2004): PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black- and-White cattle. Arc Tierzucht, 47, 557-564.
  • Edriss MA, Edriss V, Rahmani HR (2009): Association of PIT-1 gene polymorphism with birth weight, milk and reproduction traits in Isfahan Holstein cows. Arch Tierzucht, 52, 445-447.
  • Heidari M, Azari M, Hasani S, et al. (2012): Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows. Russ J Genet, 48, 417-421.
  • Hua GH, Chen SL, Yu JN, et al (2009): Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci, 81, 391-395.
  • Javanmard A, Khaledi K, Asadzadeh N, et al. (2010): Detection of polymorphism in the bovine leptin (LEP) gene: Association of a single nucleotide polymorphism with breeding value of milk traits in Iranian Holstein cattle. J Mol Genet, 2, 10-14.
  • Javanmard A, Mohammadabadi MR, Zarrigabayi GE, et al. (2008): Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russ J Genet, 44, 495-497.
  • Jiang Z, Gibson JP (1999): Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm Genome, 10, 191-193.
  • Kaçar C, Arı UÇ (2007): Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Univ Vet Fak, 13, 209-213.
  • Kadokawa H, Blache D, Yamada Y, et al. (2000): Relationships concentrations of leptin before and after parturition and the timing of first post-partum ovulation in high-producing Holstein dairy cows. Reprod Fertil Dev, 12, 405-411.
  • Khatami SR, Lazebnyĭ OE, Maksimenko VF, et al. (2005): Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black and White cattle. Genetika, 41, 229- 236.
  • Kulig H, Kmiec M (2009): Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russ J Genet, 45, 738-741.
  • Liefers SC, te Pas MF, Veerkamp RF, et al. (2002): Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J Dairy Sci, 85, 633-1638.
  • Liefers SC, Veerkamp RF, te Pas MF, et al. (2005): Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim Genet, 36, 111-118.
  • Litwinczuk Z, Krol J (2002): Polymorphism of main milk proteins in beef cattle maintained in East-Central Poland. Anim Sci Pap Rep, 1, 33-40.
  • Mangalam HJ, Albert VR, Ingraham HA, et al. (1989): A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally. Gene Dev, 3, 946-958.
  • Mattos KK, Del Lama SN, Martinez ML, et al. (2004): Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq Agropec Bras, 39, 147-150.
  • Moody DE, Pomp D, Barendse W (1995): Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Anim Genet, 26, 45-47.
  • Moravčíková N, Trakovická A, Kasarda R (2012): Polymorphism within the intron region of the bovine leptin gene in Slovak Pinzgau cattle. J Anim Sci Biotechnol, 45, 211-214.
  • Moussavi A, Ahouei M, Nassiry M, et al. (2006): Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cows. Asian-Aust J Anim Sci, 19, 627-631.
  • Nei M (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
  • Oikonomou G, Angelopoulou K, Arsenos G, et al. (2008): The effects of polymorphisms in the DGAT1, leptin and growth hormone receptor gene loci on body energy, blood metabolic and reproductive traits of Holstein cows. Anim Genet, 40, 10-17.
  • Oshima S, Barreras A (2003): Relationships between DGAT1 and Pit-1 genes polymorphism and milk yield in Holstein cattle. J Anim Sci, 54, 1-3.
  • Özdemir M (2011): Genetic polymorphism of leptin gene in Holstein and native East Anatolian Red (EAR)cattle raised as genetic resource in Turkey. Afr J Agric Res, 6, 6008-6010.
  • Passos DT, Hepp D, Moraes JCF, et al. (2007): Effect of polymorphisms linked to LEP gene on its expression on adipose tissues in beef cattle. J Anim Breed Genet, 124, 157-162.
  • Pfaffle RW, Dimattia GE, Parks JS, et al. (1992): Mutation of the Pou-specific domain of Pit-1 and hypopituitarism without pituitary hypoplasia. Science, 257, 1118-1121.
  • Pomp D, Zou T, Clutter AC, Barendse W (1997): Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J Anim Sci, 75, 1427.
  • Renaville R, Gengler N, Vrech E, et al. (1997): Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. J Dairy Sci, 80, 3431-3438.
  • Sambrook J, Fritsch E, Maniatis T (1989): Molecular cloning: A laboratory manual, (2nd) ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Santos-Alvarez J, Goberna R, Sanchez-Maralet V (1999): Human activation of human circulating monocytes. Cell Immunol, 194, 6-11. proliferation and
  • Sharifzadeh A, Doosti A (2010): Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. J Microbiol Res, 4, 1343-1345.
  • Silva DB, Crispim BA, Silva LE, et al. (2014): Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle. Genet Mol Res, 13, 3002- 3012.
  • Unanian MM, Barreto CC, Cordeiro CMT, et al. (2002): Possible associations between bovine growth hormone gene polymorphism and reproductive traits. Braz Arch Biol Techn, 45, 293-299.
  • Viorica C (2006): RFLP/HinfI polymorphism between exon 5 and exon 6 of the PIT-1 gene in Romanian Simmental cattle. Usamv Journal-Cn, 63, 219-222.
  • Yan L, Fang X, Zhang R, et al. (2011): Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle. J Appl Anim Res, 39, 269-274.
  • Yang D, Chen H, Wang X, et al. (2007): Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics, 34, 400-405.
  • Yeh FC, Yang R, Boyle T (1999): POPGENE Version 1.31. Microsoft Window-based free software for Population Genetic Analysis, University of Alberta. Edmonton, AB, Canada.
  • Zhang CL, Liu B, Chen H, et al. (2009): Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle. Anim Biotechnol, 20, 71-74.
  • Zhang L, Liu J, Zhao F, et al. (2013): Genome-wide association studies for growth and meat production traits in sheep. PloS one, 8, e66569. Geliş tarihi: 05.01.2016 / Kabul tarihi: 14.10.2016 Address for correspondence: Assist. Prof. Dr. Davut BAYRAM Erciyes University, Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandary, Kayseri, Turkey. e-mail: vetdavut@gmail.com

Türkiye’de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü- 1 (PIT-1) veleptin (LEP) gen polimorfizmlerinin belirlenmesi

Year 2017, Volume: 64 Issue: 4, 337 - 343, 01.12.2017

Davut Bayram Korhan Arslan Bilal Akyüz Kaan Muhsin İşcan

https://doi.org/10.1501/Vetfak_0000002818
Cited By: 1

Abstract

Bu çalışmada Türkiye’de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç’lik ve LEP geni için 422 bç’lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X2:40.75, p<0.001ve X2:8.4, p<0.01)

Keywords

Holştayn leptin pit-1 polimorfizm RFLP

References

  • Almeida S, Almeida E, Moraes J, et al. (2003): Molecular markers in the LEP gene and reproductive performance of beef cattle. J Anim Breed Genet, 120, 106-113
  • Aytekin I, Boztepe S (2013): Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J Anim Plant Sci, 23, 1281-1289.
  • Block S, Butler W, Bell A, et al. (2001): Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol, 171, 339-348.
  • Buchanan F, Van Kessel A, Waldner C, et al. (2003): Hot topic: An association between a leptin single nucleotide polymorphism and milk and protein yield. J Dairy Sci, 86, 3164-3166.
  • Clempson AM, Pollott GE, Brickell JS, et al. (2011): Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. J Dairy Sci, 94, 3618-3628.
  • Cohen LE, Wondisford FE, Radovick S (1997): Role of Pit-I in the gene expression of growth hormone, prolactin, and thyrotropin. Endocrinol Metab Clin N Am, 25, 23-540.
  • Cosier V, Vlaic A, Gaboreanu I (2007): HinfI polymorphism of k-casein and Pit1 genes in Romanian Simmental cattle. Sci P Anim Sci Bio, 40, 59-64.
  • Dario C, Selvaggi M, Carnicella D, et al. (2009): Bufano G: STAT5A/Aval polymorphism in Podolica bulls and its effect on growth performance traits. Livest Sci, 123, 83-87.
  • Doosti A, Arshi A, Bomeni B (2011): Molecular study of PIT1 gene polymorphism in Holstein and Iranian native cattle. Ajar, 6, 4467-4470.
  • Dybus A, Szatkowska I, Czerniawska-Piatkowska E, et al. (2004): PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black- and-White cattle. Arc Tierzucht, 47, 557-564.
  • Edriss MA, Edriss V, Rahmani HR (2009): Association of PIT-1 gene polymorphism with birth weight, milk and reproduction traits in Isfahan Holstein cows. Arch Tierzucht, 52, 445-447.
  • Heidari M, Azari M, Hasani S, et al. (2012): Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows. Russ J Genet, 48, 417-421.
  • Hua GH, Chen SL, Yu JN, et al (2009): Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci, 81, 391-395.
  • Javanmard A, Khaledi K, Asadzadeh N, et al. (2010): Detection of polymorphism in the bovine leptin (LEP) gene: Association of a single nucleotide polymorphism with breeding value of milk traits in Iranian Holstein cattle. J Mol Genet, 2, 10-14.
  • Javanmard A, Mohammadabadi MR, Zarrigabayi GE, et al. (2008): Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russ J Genet, 44, 495-497.
  • Jiang Z, Gibson JP (1999): Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm Genome, 10, 191-193.
  • Kaçar C, Arı UÇ (2007): Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Univ Vet Fak, 13, 209-213.
  • Kadokawa H, Blache D, Yamada Y, et al. (2000): Relationships concentrations of leptin before and after parturition and the timing of first post-partum ovulation in high-producing Holstein dairy cows. Reprod Fertil Dev, 12, 405-411.
  • Khatami SR, Lazebnyĭ OE, Maksimenko VF, et al. (2005): Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black and White cattle. Genetika, 41, 229- 236.
  • Kulig H, Kmiec M (2009): Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russ J Genet, 45, 738-741.
  • Liefers SC, te Pas MF, Veerkamp RF, et al. (2002): Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J Dairy Sci, 85, 633-1638.
  • Liefers SC, Veerkamp RF, te Pas MF, et al. (2005): Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim Genet, 36, 111-118.
  • Litwinczuk Z, Krol J (2002): Polymorphism of main milk proteins in beef cattle maintained in East-Central Poland. Anim Sci Pap Rep, 1, 33-40.
  • Mangalam HJ, Albert VR, Ingraham HA, et al. (1989): A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally. Gene Dev, 3, 946-958.
  • Mattos KK, Del Lama SN, Martinez ML, et al. (2004): Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq Agropec Bras, 39, 147-150.
  • Moody DE, Pomp D, Barendse W (1995): Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Anim Genet, 26, 45-47.
  • Moravčíková N, Trakovická A, Kasarda R (2012): Polymorphism within the intron region of the bovine leptin gene in Slovak Pinzgau cattle. J Anim Sci Biotechnol, 45, 211-214.
  • Moussavi A, Ahouei M, Nassiry M, et al. (2006): Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cows. Asian-Aust J Anim Sci, 19, 627-631.
  • Nei M (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
  • Oikonomou G, Angelopoulou K, Arsenos G, et al. (2008): The effects of polymorphisms in the DGAT1, leptin and growth hormone receptor gene loci on body energy, blood metabolic and reproductive traits of Holstein cows. Anim Genet, 40, 10-17.
  • Oshima S, Barreras A (2003): Relationships between DGAT1 and Pit-1 genes polymorphism and milk yield in Holstein cattle. J Anim Sci, 54, 1-3.
  • Özdemir M (2011): Genetic polymorphism of leptin gene in Holstein and native East Anatolian Red (EAR)cattle raised as genetic resource in Turkey. Afr J Agric Res, 6, 6008-6010.
  • Passos DT, Hepp D, Moraes JCF, et al. (2007): Effect of polymorphisms linked to LEP gene on its expression on adipose tissues in beef cattle. J Anim Breed Genet, 124, 157-162.
  • Pfaffle RW, Dimattia GE, Parks JS, et al. (1992): Mutation of the Pou-specific domain of Pit-1 and hypopituitarism without pituitary hypoplasia. Science, 257, 1118-1121.
  • Pomp D, Zou T, Clutter AC, Barendse W (1997): Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J Anim Sci, 75, 1427.
  • Renaville R, Gengler N, Vrech E, et al. (1997): Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. J Dairy Sci, 80, 3431-3438.
  • Sambrook J, Fritsch E, Maniatis T (1989): Molecular cloning: A laboratory manual, (2nd) ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Santos-Alvarez J, Goberna R, Sanchez-Maralet V (1999): Human activation of human circulating monocytes. Cell Immunol, 194, 6-11. proliferation and
  • Sharifzadeh A, Doosti A (2010): Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. J Microbiol Res, 4, 1343-1345.
  • Silva DB, Crispim BA, Silva LE, et al. (2014): Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle. Genet Mol Res, 13, 3002- 3012.
  • Unanian MM, Barreto CC, Cordeiro CMT, et al. (2002): Possible associations between bovine growth hormone gene polymorphism and reproductive traits. Braz Arch Biol Techn, 45, 293-299.
  • Viorica C (2006): RFLP/HinfI polymorphism between exon 5 and exon 6 of the PIT-1 gene in Romanian Simmental cattle. Usamv Journal-Cn, 63, 219-222.
  • Yan L, Fang X, Zhang R, et al. (2011): Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle. J Appl Anim Res, 39, 269-274.
  • Yang D, Chen H, Wang X, et al. (2007): Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics, 34, 400-405.
  • Yeh FC, Yang R, Boyle T (1999): POPGENE Version 1.31. Microsoft Window-based free software for Population Genetic Analysis, University of Alberta. Edmonton, AB, Canada.
  • Zhang CL, Liu B, Chen H, et al. (2009): Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle. Anim Biotechnol, 20, 71-74.
  • Zhang L, Liu J, Zhao F, et al. (2013): Genome-wide association studies for growth and meat production traits in sheep. PloS one, 8, e66569. Geliş tarihi: 05.01.2016 / Kabul tarihi: 14.10.2016 Address for correspondence: Assist. Prof. Dr. Davut BAYRAM Erciyes University, Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandary, Kayseri, Turkey. e-mail: vetdavut@gmail.com
There are 47 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA59GT96VH
Journal Section Research Article
Authors

Davut Bayram

Korhan Arslan

Bilal Akyüz

Kaan Muhsin İşcan

Publication Date December 1, 2017
Published in Issue Year 2017Volume: 64 Issue: 4

Cite

APA Bayram, D., Arslan, K., Akyüz, B., İşcan, K. M. (2017). Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(4), 337-343. https://doi.org/10.1501/Vetfak_0000002818
AMA Bayram D, Arslan K, Akyüz B, İşcan KM. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Univ Vet Fak Derg. December 2017;64(4):337-343. doi:10.1501/Vetfak_0000002818
Chicago Bayram, Davut, Korhan Arslan, Bilal Akyüz, and Kaan Muhsin İşcan. “Identification of Pituitary-Specific Transcription Factor-1 (PIT-1) and Leptin Gene (LEP) Polymorphism of Holstein Cattle Reared in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64, no. 4 (December 2017): 337-43. https://doi.org/10.1501/Vetfak_0000002818.
EndNote Bayram D, Arslan K, Akyüz B, İşcan KM (December 1, 2017) Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 4 337–343.
IEEE D. Bayram, K. Arslan, B. Akyüz, and K. M. İşcan, “Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey”, Ankara Univ Vet Fak Derg, vol. 64, no. 4, pp. 337–343, 2017, doi: 10.1501/Vetfak_0000002818.
ISNAD Bayram, Davut et al. “Identification of Pituitary-Specific Transcription Factor-1 (PIT-1) and Leptin Gene (LEP) Polymorphism of Holstein Cattle Reared in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64/4 (December 2017), 337-343. https://doi.org/10.1501/Vetfak_0000002818.
JAMA Bayram D, Arslan K, Akyüz B, İşcan KM. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Univ Vet Fak Derg. 2017;64:337–343.
MLA Bayram, Davut et al. “Identification of Pituitary-Specific Transcription Factor-1 (PIT-1) and Leptin Gene (LEP) Polymorphism of Holstein Cattle Reared in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 64, no. 4, 2017, pp. 337-43, doi:10.1501/Vetfak_0000002818.
Vancouver Bayram D, Arslan K, Akyüz B, İşcan KM. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Univ Vet Fak Derg. 2017;64(4):337-43.

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