Investigation of the diagnostic value of serology, cytology and polymerase chain reaction in cat toxoplasmosis
Abstract
Toxoplasmosis is an infectious disease affecting most of the mammalian and aviary animals caused by Toxoplasma gondii. In this study, diagnostic values of bronchoalveolar lavage, biopsy and PCR and their practical implementation ways were questioned. The results of the each test were evaluated. Additionally, incidence of toxoplasmosis among seropositive cats were investigated by T. gondii and oocyst shedding screened in the stool samples, as well. The material of the study was constituted by blood, stool and bronchoalveoloar lavage samples taken from totally 102 cats. Bronchoalveolar lavage samples were evaluated for cytologic examination. In PCR analysis, T. gondii B1 primary sets were used and conventional-PCR procedure was applied. Antibodies to T. gondii were found in 49 (48,03%) of 102, with titers of 1/16 in 41 (40,19%), 1/64 in 8 (7,84%) cats. Only one cat exhibited systemic toxoplasmosis. In conclusion, although the seroprevalance was 48.03%, systemic toxoplasmosis rate was found 0.98% (1/49) with a lower percentage
Keywords
References
- Averill DR Jr, De Lahunta A (1971): Toxoplasmosis of the canine nervous system: Clinicopathologic findings in four cases. J Am Vet Med Assoc, 159, 1134-1141.
- Babür C, Aktaş M, Dumanlı N ve ark. (1998): Elazığ yöresinde kedilerde Sabin-Feldman boya testi ile anti- Toxoplasma gondii antikorlarının araştırılması. Vet Bil Derg, 14, 55-58.
- Babür C, Pişkin FÇ, Bıyıkoğlu G ve ark. (1999): Eskişehir Çifteler harası keçilerinde anti-Toxoplasma gondii antikorlarının Sabin-Feldman boya testi (SFDT) ile araştırılması. T Parazitol Derg, 23, 72-74.
- Can H, Doskaya M, Ajzenberg D ve ark. (2014): Genetic characterization of Toxoplasma gondii isolates and Toxoplasmosis seroprevalence in stray cats of ̇İzmir, Turkey. PLoS ONE 9 (8): e104930. doi:10.1371/journal. pone. 0104930.
- Cunningham AA, Buxton D, Thomson KM (1992): An epidemic of toxoplasmosis in a captive colony of squirrel monkeys (Saimiri sciureus). J Comp Pathol, 107, 207-219.
- Deeb BJ, Sufan MM, Digiacomo RF (1985): Toxoplasma gondii infection of cats in Beirut, Lebanon. J Trop Med Hyg, 88, 301-306.
- Dubey JP, Frenkel JK (1974): Immunity to feline toxoplasmosis: corticosteroids. Vet Pathol, 11, 350-379. by administration of
- Dubey JP (1986): Toxoplasmosis. JAVMA, 189,166-170.
- Dubey JP, Beattie CP (1988): Toxoplasmosis of animals and man. CRC Press, Inc, Boca Raton, Florida, p: 1-26.
- Dubey JP, Carpenter JL (1993): Histologically confirmed clinical toxoplasmosis in cats: 100 cases (1952-1990). J Am Vet Med Assoc, 203, 1556-66.
- Dubey JP (1995): Duration of immunity to shedding of Toxoplasma gondii oocysts by cats. J Parasitol, 81, 410-415.
- Dubey JP, Mattix ME, Lipscomb TP (1996): Lesions of neonatally induced toxoplasmosis in cats. Vet Pathol, 33, 290-295.
- Dubey JP (2004): Toxoplasmosis-a waterborne zoonosis. Vet Parasitol, 126, 57-72.
- Dubey JP (2010): Toxoplasmosis of animals and man. CRC Press, Boca Raton, Florida, p:1-220.
- Fostera SF, Martin P, Braddock JA ve ark. (2004): Retrospective analysis of feline bronchoalveolar lavage cytology and microbiology (1995-2000). J Feline Med Surg, 6, 189-198.
- Frenkel JK (1990): Transmission of toxoplasmosis and the role of immunity in limiting transmission and illness. JAVMA, 196, 233-240.
- Hazıroğlu R, Altınsaat MS, Atasever A ve ark. (1988): Kedilerde fatal toksoplazmozis. AÜ Vet Fak Derg, 35, 330- 340.
- Hazıroğlu R (1993): An ultrastructural study of Toxoplasma gondii developmental stages in the lungs of the cat. Isr J Vet Med, 48, 65-68.
- Hazıroglu R, Gülbahar MY, Altıntaş K (1994): Demonstration of Toxoplasma gondii antigen in naturally- infected cats using immunoperoxidase technique. Isr J Vet Med, 49, 28-30.
- Henriksen P, Dietz HH, Uttenthal A ve ark. (1994): Seroprevalence of Toxoplasma gondii in Danish farmed mink (Mustela vison S.). Vet Parasitol, 53, 1-5.
- Keagy HF (1949): Toxoplasma in the chinchilla. JAVMA, 114, 15.
- Kul O, Atmaca HT, Deniz A ve ark. (2011): Clinicopathologic diagnosis of cutaneous toxoplasmosis in an Angora cat. Berl Munch Tierarztl Wochenschr, 124, 386-9.
- Levine ND (1977): Taxonomy of Toxoplasma. J Protozool, 24, 36-41.
- Ocholi RA, Kalejaiye JO, Okewole PA (1989): Acute disseminated toxoplasmosis in two captive lions (Panthera leo) in Nigeria. Vet Rec, 124, 515-516.
- Olafson P, Monlux WS (1942): Toxoplasma infections in animals. Cornell Vet, 32, 16-190.
- Owen MR, Clarkson MJ, Trees AJ (1998): Diagnosis of toxoplasma abortion in ewes by polymerase chain reaction. Vet Rec, 142, 445-448.
- Özkan AT, Celebi B, Babür C ve ark. (2008): Investigation of anti-Toxoplasma gondii antibodies in cats of the Ankara region of Turkey. J Parasitol, 48, 236-9.
- Patton S, Legendre AM, McGavin MD ve ark. (1991): Concurrent infection with Toxoplasma gondii and feline leukemia virus. Antibody response and oocyst production. J Vet Intern Med, 5, 199-201.
- Tanyüksel M, Gün H, Erdal N ve ark. (1994): Toksoplazmosis karşılaştırılması. T Parazitol Derg, 18, 266-276. serolojik testlerin Geliş tarihi: 29.12.2015 / Kabul tarihi: 19.10.2016
Kedilerde toksoplazmoz tanısında seroloji, sitoloji ve polimeraz zincir reaksiyonunun tanısal değerlerinin araştırılması
Abstract
Toksoplazmozis, Toxoplasma gondii tarafından meydana getirilen, çok sayıda memeli ve kanatlı hayvanda görülen, protozoer bir enfeksiyondur. Bu çalışmada; klinik belirti gösteren ya da T. gondii seropozitif bulunan kedilerde uygulanabilecek bronkoalveolar lavaj ve PZR tanı yöntemlerinin geçerliliği sorgulanarak, tanısal önemleri araştırıldı. Ayrıca, seropozitif bulunan kediler arasında sistemik toksoplazmozis görülme sıklığı ile dışkıda ookist varlığı muayene edildi. Çalışma materyalini, 102 kediden alınan kan, uygun olgularda ise bronkoalveolar lavaj, deri ve karaciğer biyopsileri oluşturdu. Bu kapsamda, kan serumlarında Sabin-Feldman boya testi ile T. gondii seropozitif bulunan kediler seçilerek; anestezi altında endotrakeyal kateter yardımıyla bronkoalveolar lavaj örneğinde sitolojik muayene yapıldı. PZR analizinde ise T. gondii B1 geni spesifik primerler ile konvansiyonel-PZR prosedürleri uygulandı. Çalışmada incelenen kedilerde T. gondii seroprevalansı 49/102 (%48.03) olarak belirlendi. Anti-Toxoplasma gondii antikor titreleri açısından incelendiğinde; bunlardan %40.19’unun (41/102) 1/16, %7.84’ünün (8/102) ise 1/64 titrede olduğu ortaya konuldu. Sonuç olarak, seroprevalans %48.03 olmasına rağmen sistemik toksoplazmozis oranının (49 seropozitif kediden biri, %0.98) oldukça düşükgerçekleştiği belirlendi
Keywords
References
- Averill DR Jr, De Lahunta A (1971): Toxoplasmosis of the canine nervous system: Clinicopathologic findings in four cases. J Am Vet Med Assoc, 159, 1134-1141.
- Babür C, Aktaş M, Dumanlı N ve ark. (1998): Elazığ yöresinde kedilerde Sabin-Feldman boya testi ile anti- Toxoplasma gondii antikorlarının araştırılması. Vet Bil Derg, 14, 55-58.
- Babür C, Pişkin FÇ, Bıyıkoğlu G ve ark. (1999): Eskişehir Çifteler harası keçilerinde anti-Toxoplasma gondii antikorlarının Sabin-Feldman boya testi (SFDT) ile araştırılması. T Parazitol Derg, 23, 72-74.
- Can H, Doskaya M, Ajzenberg D ve ark. (2014): Genetic characterization of Toxoplasma gondii isolates and Toxoplasmosis seroprevalence in stray cats of ̇İzmir, Turkey. PLoS ONE 9 (8): e104930. doi:10.1371/journal. pone. 0104930.
- Cunningham AA, Buxton D, Thomson KM (1992): An epidemic of toxoplasmosis in a captive colony of squirrel monkeys (Saimiri sciureus). J Comp Pathol, 107, 207-219.
- Deeb BJ, Sufan MM, Digiacomo RF (1985): Toxoplasma gondii infection of cats in Beirut, Lebanon. J Trop Med Hyg, 88, 301-306.
- Dubey JP, Frenkel JK (1974): Immunity to feline toxoplasmosis: corticosteroids. Vet Pathol, 11, 350-379. by administration of
- Dubey JP (1986): Toxoplasmosis. JAVMA, 189,166-170.
- Dubey JP, Beattie CP (1988): Toxoplasmosis of animals and man. CRC Press, Inc, Boca Raton, Florida, p: 1-26.
- Dubey JP, Carpenter JL (1993): Histologically confirmed clinical toxoplasmosis in cats: 100 cases (1952-1990). J Am Vet Med Assoc, 203, 1556-66.
- Dubey JP (1995): Duration of immunity to shedding of Toxoplasma gondii oocysts by cats. J Parasitol, 81, 410-415.
- Dubey JP, Mattix ME, Lipscomb TP (1996): Lesions of neonatally induced toxoplasmosis in cats. Vet Pathol, 33, 290-295.
- Dubey JP (2004): Toxoplasmosis-a waterborne zoonosis. Vet Parasitol, 126, 57-72.
- Dubey JP (2010): Toxoplasmosis of animals and man. CRC Press, Boca Raton, Florida, p:1-220.
- Fostera SF, Martin P, Braddock JA ve ark. (2004): Retrospective analysis of feline bronchoalveolar lavage cytology and microbiology (1995-2000). J Feline Med Surg, 6, 189-198.
- Frenkel JK (1990): Transmission of toxoplasmosis and the role of immunity in limiting transmission and illness. JAVMA, 196, 233-240.
- Hazıroğlu R, Altınsaat MS, Atasever A ve ark. (1988): Kedilerde fatal toksoplazmozis. AÜ Vet Fak Derg, 35, 330- 340.
- Hazıroğlu R (1993): An ultrastructural study of Toxoplasma gondii developmental stages in the lungs of the cat. Isr J Vet Med, 48, 65-68.
- Hazıroglu R, Gülbahar MY, Altıntaş K (1994): Demonstration of Toxoplasma gondii antigen in naturally- infected cats using immunoperoxidase technique. Isr J Vet Med, 49, 28-30.
- Henriksen P, Dietz HH, Uttenthal A ve ark. (1994): Seroprevalence of Toxoplasma gondii in Danish farmed mink (Mustela vison S.). Vet Parasitol, 53, 1-5.
- Keagy HF (1949): Toxoplasma in the chinchilla. JAVMA, 114, 15.
- Kul O, Atmaca HT, Deniz A ve ark. (2011): Clinicopathologic diagnosis of cutaneous toxoplasmosis in an Angora cat. Berl Munch Tierarztl Wochenschr, 124, 386-9.
- Levine ND (1977): Taxonomy of Toxoplasma. J Protozool, 24, 36-41.
- Ocholi RA, Kalejaiye JO, Okewole PA (1989): Acute disseminated toxoplasmosis in two captive lions (Panthera leo) in Nigeria. Vet Rec, 124, 515-516.
- Olafson P, Monlux WS (1942): Toxoplasma infections in animals. Cornell Vet, 32, 16-190.
- Owen MR, Clarkson MJ, Trees AJ (1998): Diagnosis of toxoplasma abortion in ewes by polymerase chain reaction. Vet Rec, 142, 445-448.
- Özkan AT, Celebi B, Babür C ve ark. (2008): Investigation of anti-Toxoplasma gondii antibodies in cats of the Ankara region of Turkey. J Parasitol, 48, 236-9.
- Patton S, Legendre AM, McGavin MD ve ark. (1991): Concurrent infection with Toxoplasma gondii and feline leukemia virus. Antibody response and oocyst production. J Vet Intern Med, 5, 199-201.
- Tanyüksel M, Gün H, Erdal N ve ark. (1994): Toksoplazmosis karşılaştırılması. T Parazitol Derg, 18, 266-276. serolojik testlerin Geliş tarihi: 29.12.2015 / Kabul tarihi: 19.10.2016
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA92HT42TF |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2017 |
| Published in Issue | Year 2017Volume: 64 Issue: 3 |