Letter to Editor
First isolation of Vibrio furnissii (emerging Vibrio) from mussels (Mediterranean mussel and bearded mussel) in Turkey
Abstract
Vibrio furnissii is an emerging pathogen that can cause acute gastroenteritis in humans. In this work, 652 bivalve mollusc samples (wedge shell-Donax trunculus, L.1758, oyster-Ostrea edulis, L.1758, cockle-Venus verrucosa, L.1758, clam-Tapes decussatus, L.1758, bearded mussel-Modiolus barbatus, L.1758, Mediterranean mussel-Mytilus galloprovincialis L.1819, striped venus-Chamelea gallina L.1753) sent to the laboratory from stations in Ayvalık and Balıkesir (Northern Aegean Region of Turkey) between 2007-2010 were researched with conventional microbiological methods and six isolates were identified as Vibrio furnisii. Isolates were later confirmed with PCR using primers specific to toxR gene. Isolation rate of V. furnisii from bivalve molluscs per years were 2007 (2%), 2008 (0.43%), 2009 (1.29%), 2010 (0%) and per shell fish species were mediterranean mussel (3.33%), bearded mussel (3.27%), oyster (3.27%), clam (0%), cockle (0%), wedge shell (0%), striped venus (0 %). In this work, V. furnissi was isolated from mussels in Turkey for the first time and attention was drawn zoonotic importance of the agent
References
- Ahsan CR, Hoque MM, Rasul Z, et al. (1992): Enterotoxicity of Vibrio furnissii isolated from eels. World J Microbial Biotech, 8, 187-189.
- Amin MAM, Rizk EGW, Mohamed TS (2011): Occurrence of some zoonotic Vibrios in shellfish and diarrheic patients with regard to tdh gene in Vibrio parahaemolyticus. J Am Sci, 7, 449- 459.
- Austin B (2010): Vibrios as causal agents of zoonoses. Vet Microbiol, 140, 310-317. B, 4. Austin Austin DA representatives: Characteristics of the disease. In: Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. Springer-Praxis, Chichester 29-30, 108-115. Vibrionaceae
- Avsever ML (2016): First report of trh positive Vibrio alginolyticus isolates from bivalve molluscs in Turkey. Revue Méd Vét, 167, 65-70.
- Azevedo C, Villalba A (1991): Extracellular giant rickettsiae associated with bacteria in the gill of Crassostrea gigas (Mollusca, Bivalvia). J Invertebr Pathol, 58, 75-81.
- Baumann P, Baumann L, Woolkalis MJ, et al. (1983): Evolutionary relationships in vibrio and Photobacterium: A basis fora natural classification. Annu Rev Microbiol, 37, 369-398.
- Brenner DJ, Hickman-Brenner FW, Lee JV (1983): Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis), a new species isolated from human feces and the environment. J Clin Microbiol, 61, 437-467.
- Chakraborty S, Nair GB, Shinoda S (1997): Pathogenic vibrios in the natural aquatic environment. Rev Environ Health 12, 63-80.
- Dalsgaard A, Glerup P, Hİybye LL, et al. (1997): Vibrio furnissii isolated from humans in Peru: A possible human pathogen. Epidemiol Infect, 119, 143-149.
- Demir Y (2012): Türkiye’de ihraç edilen Bivalve türlerinden Vibrio türlerinin izolasyon ve identifikasyonu. Etlik Vet Mik Derg, 23, 1-8
- Espineira M, Atanassova M, Vieites JM, et al. (2010): Validation of a method for the detection of five species, serogroups, biotypes and virulence factors of Vibrio by multiplex PCR in fish and seafood. Food Microbiol, 27, 122- 131.
- Esteve C, Amaro C, Biosca EG, et al. (1995): Biochemical and toxigenic properties of Vibrio furnissii isolated from a European eel farm. Aquaculture, 132, 81-90.
- Furniss AL, Lee JV, Donovan TJ (1977): Group F, a new Vibrio? Lancet, 2, 565-566.
- Ghadersohi A, Owens L (1999): Isolation, characterisation and DNA analysis of Mycoplasma spp. from moribund prawns Penaeus monodon cultured in Australia. Dis Aquat Org, 35, 53-61.
- Hickman-Brenner FW, Brenner DJ, Steıgerwalt AG, et al. (1984): Vibrio fluvialis and Vibrio furnissii isolated from a stool sample of one patient. J Clin Microbiol, 20, 125-127.
- Igbinosa EO, Okoh AI (2008): Emerging Vibrio species: An unending threat to public health in developing countries. Res Microbiol, 159, 495-506.
- Krieg NR, Holt JG (1984): Bergey's manual of systematic bacteriology, vol. Williams & Wilkins, Baltimore. Md.
- Lee JV, Shread P, Furniss AL, et al. (1981): Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F Vibrios, group EF-6. J Appl Bacteriol, 50, 73-94.
- Lux TM, Lee R, Love J (2011): Complete genome sequence of a free-living Vibrio furnissii sp. nov. strain (NCTC 11218). J Bacteriol, 193, 1487-1488.
- Lux TM, Lee R, Love J (2014): Genome-wide phylogenetic analysis of the pathogenic potential of Vibrio furnissii. Front Microbiol, 5, 435.
- Magalhaes V, Castello Filho A, Magalhaes M, et al. (1993): Laboratory evaluation on pathogenic potentialities of Vibrio furnissii. Mem Inst Oswaldo, 88, 593-597.
- Mathur J, Waldor MK (2004): The Vibrio cholerae ToxR- regulated porin OmpU confers resistance to antimicrobial peptides. Infect Immun, 72, 3577-3583.
- Matte GR, Matte MH, Rivera ING, et al. (1994): Distribution of potentially pathogenic Vibrios in Oysters from a tropical region. J Food Prot, 3-1, 870-873.
- Myatt DC, Davis GHG (1989): Extracellular and surface- bound biological activities of Vibrio fluvialis, Vibrio furnissii and related species. Med Microbiol lmmun, 178, 279-287.
- Renault T, Cochennec N (1995): Chlamydia-like organisms in ctenidia and mantle cells of the Japanese oyster Crassostrea gigas from the French Atlantic coast. Dis Aquat Org, 23, 153-159.
- Schirmeister F, Dieckmann R, Bechlars S, et al. (2013): Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 400 isolated from German and Austrian patients. Eur J Clin Microbiol Infect Dis, 33, 767-78.
- Schirmeistera F, Wieczoreka A, Dieckmanna R, et al. (2014): Evaluation of molecular methods to discriminate the closely related species Vibrio fluvialis and Vibrio furnissii. Int J Med Microbiol, 304, 851-857.
- Sung HH, Hsu SF, Chen CK, et al. (2001): Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture, 192, 101-110.
- Terzi G, Büyüktanır Ö, Yurdusev N (2009): Detection of the tdh and trh genes in Vibrio parahaemolyticus isolates in fish and mussels from middle black sea coast of Turkey. Lett Appl Microbiol, 49, 757-763.
- Tubiash HS, Otto SV, Hugh R (1973): Cardiac edema associated with Vibrio anguillarum in the American oyster. Proc Natl Shellfish Assoc, 63, 39-42.
- Türk N, Avsever ML, Ün C (2011): Investigation of tdh and trh genes in Vibrio parahaemolyticus isoletes from isolated Bivalve molluscs. Bor Vet Bil Der, 33, 29-36.
- Wieczoreka A, Dieckmanna R, Taureck K, et al. (2014): Evaluation of molecular methods to discriminate the closely related species Vibrio fluvialis and Vibrio furnissii Falko Schirmeistera. Int J Med Microbiol, 304, 851-857.
- Wu TK, Wang YK, Chen YC, et al. (2007): Identification of a Vibrio furnissii oligopeptide permease and characterization fits in vitro hemolytic activity. J Bacteriol, 189, 8215-8223. Geliş tarihi: 28.12.2015 / Kabul tarihi: 22.06.2016 Address for correspondence: Dr. Meriç Lütfi Avsever Aksaray University, Eskil Vocation of High School, Laboratory and Veterinary Sciences, 68800, Eskil Aksaray,Turkey. e-mail: lutfiavsever@gmail.com
Türkiye’deki midyelerden (Kara midye ve kıllı midye) ilk Vibrio furnissii (emerging Vibrio) izolasyonu
Abstract
Vibrio furnissii insanlarda akut gastroenteritise sebep olan ve önemi gittikçe artan bir patojendir. Bu çalışmada, 20072010 yılları arasında, Ayvalık ve Balıkesir’deki (Türkiye’nin Kuzey Ege bölgesi) istasyonlardan laboratuvara gönderilmiş 652 takım çift kabuklu yumuşakça (kum şırlanı-Donax trunculus, L. 1758, yassı istiridye-Ostrea edulis, L. 1758, kidonya-Venus verrucosa, L. 1758, akivades-Tapes decussatus, L. 1758, kıllı midye -Modiolus barbatus, L. 1758, kara midye - Mytilus galloprovincialis L. 1819, kum midyesi, Chamelea gallina L.1753) konvansiyonel mikrobiyolojik yöntemlerle araştırıldı ve çalışmada izole edilen altı izolatın V. furnissi olduğu tespit edildi. İzolatlar daha sonra toxR genine spesifik primerler kullanılarak PCR ile konfirme edildi. Çalışmada kullanılan çift kabuklu yumuşakçalardan V. furnissii izolasyon oranı yıl ve tür bazında 2007 (% 2), 2008 (% 0.43), 2009 (% 1.29), 2010 (% 0); kara midye (% 3.33), kıllı midye (% 3.27), istiridye (% 3.27), akivades (% 0), kidonya (% 0), kum şırlanı (% 0), kum midyesi (% 0) olarak tespit edildi. Bu çalışmada Türkiye’deki midyelerden ilk kez V. furnissi izole edilmiş olup, etkenin zoonotik önemi vurgulanmıştır
References
- Ahsan CR, Hoque MM, Rasul Z, et al. (1992): Enterotoxicity of Vibrio furnissii isolated from eels. World J Microbial Biotech, 8, 187-189.
- Amin MAM, Rizk EGW, Mohamed TS (2011): Occurrence of some zoonotic Vibrios in shellfish and diarrheic patients with regard to tdh gene in Vibrio parahaemolyticus. J Am Sci, 7, 449- 459.
- Austin B (2010): Vibrios as causal agents of zoonoses. Vet Microbiol, 140, 310-317. B, 4. Austin Austin DA representatives: Characteristics of the disease. In: Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. Springer-Praxis, Chichester 29-30, 108-115. Vibrionaceae
- Avsever ML (2016): First report of trh positive Vibrio alginolyticus isolates from bivalve molluscs in Turkey. Revue Méd Vét, 167, 65-70.
- Azevedo C, Villalba A (1991): Extracellular giant rickettsiae associated with bacteria in the gill of Crassostrea gigas (Mollusca, Bivalvia). J Invertebr Pathol, 58, 75-81.
- Baumann P, Baumann L, Woolkalis MJ, et al. (1983): Evolutionary relationships in vibrio and Photobacterium: A basis fora natural classification. Annu Rev Microbiol, 37, 369-398.
- Brenner DJ, Hickman-Brenner FW, Lee JV (1983): Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis), a new species isolated from human feces and the environment. J Clin Microbiol, 61, 437-467.
- Chakraborty S, Nair GB, Shinoda S (1997): Pathogenic vibrios in the natural aquatic environment. Rev Environ Health 12, 63-80.
- Dalsgaard A, Glerup P, Hİybye LL, et al. (1997): Vibrio furnissii isolated from humans in Peru: A possible human pathogen. Epidemiol Infect, 119, 143-149.
- Demir Y (2012): Türkiye’de ihraç edilen Bivalve türlerinden Vibrio türlerinin izolasyon ve identifikasyonu. Etlik Vet Mik Derg, 23, 1-8
- Espineira M, Atanassova M, Vieites JM, et al. (2010): Validation of a method for the detection of five species, serogroups, biotypes and virulence factors of Vibrio by multiplex PCR in fish and seafood. Food Microbiol, 27, 122- 131.
- Esteve C, Amaro C, Biosca EG, et al. (1995): Biochemical and toxigenic properties of Vibrio furnissii isolated from a European eel farm. Aquaculture, 132, 81-90.
- Furniss AL, Lee JV, Donovan TJ (1977): Group F, a new Vibrio? Lancet, 2, 565-566.
- Ghadersohi A, Owens L (1999): Isolation, characterisation and DNA analysis of Mycoplasma spp. from moribund prawns Penaeus monodon cultured in Australia. Dis Aquat Org, 35, 53-61.
- Hickman-Brenner FW, Brenner DJ, Steıgerwalt AG, et al. (1984): Vibrio fluvialis and Vibrio furnissii isolated from a stool sample of one patient. J Clin Microbiol, 20, 125-127.
- Igbinosa EO, Okoh AI (2008): Emerging Vibrio species: An unending threat to public health in developing countries. Res Microbiol, 159, 495-506.
- Krieg NR, Holt JG (1984): Bergey's manual of systematic bacteriology, vol. Williams & Wilkins, Baltimore. Md.
- Lee JV, Shread P, Furniss AL, et al. (1981): Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F Vibrios, group EF-6. J Appl Bacteriol, 50, 73-94.
- Lux TM, Lee R, Love J (2011): Complete genome sequence of a free-living Vibrio furnissii sp. nov. strain (NCTC 11218). J Bacteriol, 193, 1487-1488.
- Lux TM, Lee R, Love J (2014): Genome-wide phylogenetic analysis of the pathogenic potential of Vibrio furnissii. Front Microbiol, 5, 435.
- Magalhaes V, Castello Filho A, Magalhaes M, et al. (1993): Laboratory evaluation on pathogenic potentialities of Vibrio furnissii. Mem Inst Oswaldo, 88, 593-597.
- Mathur J, Waldor MK (2004): The Vibrio cholerae ToxR- regulated porin OmpU confers resistance to antimicrobial peptides. Infect Immun, 72, 3577-3583.
- Matte GR, Matte MH, Rivera ING, et al. (1994): Distribution of potentially pathogenic Vibrios in Oysters from a tropical region. J Food Prot, 3-1, 870-873.
- Myatt DC, Davis GHG (1989): Extracellular and surface- bound biological activities of Vibrio fluvialis, Vibrio furnissii and related species. Med Microbiol lmmun, 178, 279-287.
- Renault T, Cochennec N (1995): Chlamydia-like organisms in ctenidia and mantle cells of the Japanese oyster Crassostrea gigas from the French Atlantic coast. Dis Aquat Org, 23, 153-159.
- Schirmeister F, Dieckmann R, Bechlars S, et al. (2013): Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 400 isolated from German and Austrian patients. Eur J Clin Microbiol Infect Dis, 33, 767-78.
- Schirmeistera F, Wieczoreka A, Dieckmanna R, et al. (2014): Evaluation of molecular methods to discriminate the closely related species Vibrio fluvialis and Vibrio furnissii. Int J Med Microbiol, 304, 851-857.
- Sung HH, Hsu SF, Chen CK, et al. (2001): Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture, 192, 101-110.
- Terzi G, Büyüktanır Ö, Yurdusev N (2009): Detection of the tdh and trh genes in Vibrio parahaemolyticus isolates in fish and mussels from middle black sea coast of Turkey. Lett Appl Microbiol, 49, 757-763.
- Tubiash HS, Otto SV, Hugh R (1973): Cardiac edema associated with Vibrio anguillarum in the American oyster. Proc Natl Shellfish Assoc, 63, 39-42.
- Türk N, Avsever ML, Ün C (2011): Investigation of tdh and trh genes in Vibrio parahaemolyticus isoletes from isolated Bivalve molluscs. Bor Vet Bil Der, 33, 29-36.
- Wieczoreka A, Dieckmanna R, Taureck K, et al. (2014): Evaluation of molecular methods to discriminate the closely related species Vibrio fluvialis and Vibrio furnissii Falko Schirmeistera. Int J Med Microbiol, 304, 851-857.
- Wu TK, Wang YK, Chen YC, et al. (2007): Identification of a Vibrio furnissii oligopeptide permease and characterization fits in vitro hemolytic activity. J Bacteriol, 189, 8215-8223. Geliş tarihi: 28.12.2015 / Kabul tarihi: 22.06.2016 Address for correspondence: Dr. Meriç Lütfi Avsever Aksaray University, Eskil Vocation of High School, Laboratory and Veterinary Sciences, 68800, Eskil Aksaray,Turkey. e-mail: lutfiavsever@gmail.com
There are 33 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA58MH34UM |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2017 |
| Published in Issue | Year 2017Volume: 64 Issue: 3 |