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Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province

Year 2017, Volume: 64 Issue: 1, 51 - 54, 01.03.2017

Sami Gökpınar Rukiye Değirmenci Kader Yıldız

https://doi.org/10.1501/Vetfak_0000002773
Cited By: 2

Abstract

Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus. In this study, it was aimed to detection strain identification of E. granulosus obtained from liver and lungs of cattle slaughtered in Kırıkkale region. For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made. In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results

Keywords

Cattle Echinococcus granulosus genotyping hydatid cyst Kırıkkale

References

  • 1. Altintas N, Oztatlici M, Altintas N ve ark. (2013): Molecular analysis of cattle isolates of Echinococcus granulosus in Manisa Province of Turkey. Kafkas Univ Vet Fak Derg, 19, 455-459.
  • 2. Beyhan YE, Umur S (2011): Molecular characterization and prevalence of cystic echinococcosis in slaughtered water buffaloes in Turkey. Vet Parasitol, 181, 174-179.
  • 3. Bowles J, Blair D, McManus DP (1992): Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol, 54, 165-173.
  • 4. Bowles J, McManus DP (1993): NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol, 23, 969-972.
  • 5. Budak A, Yıldız K, Çakır Arıca Ş ve ark. (2010): Kırıkkale yöresinde koyunlardan elde edilen Echinococcus granulosus izolatlarının moleküler karakteri. Kafkas Üniv Vet Fak Derg, 16, 245-250.
  • 6. Cardona GA, Carmena D (2013): A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Vet Parasitol, 192, 10-32.
  • 7. Dinkel A, Njoroge EM, Zimmermann A ve ark. (2004): A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol, 34, 645-653.
  • 8. Eckert J, Gemmel MA, Meslin FX ve ark. (2001): WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Organisation of Animal Health, Paris, France.
  • 9. Eckert J, Thompson RCA (1997): Intraspesific variation of Echinococcus granulosus and related species with emphises on their infectivity to humans. Acta Trop, 64, 19- 34.
  • 10. Ergin S, Saribas S, Yuksel P ve ark. (2010): Genotypic characterisation of Echinococcus granulosus isolated from human in Turkey. Afr J Microbiol Res, 4, 551-555.
  • 11. Eryildiz C, Sakru N (2012): Molecular characterization of human and animal isolates of Echinococcus granulosus in the Thrace Region, Turkey. Balkan Med J, 29, 261-267.
  • 12. Gasser RB, Zhu X, McManus DP (1998): Dideoxy fingerprinting: Application to the genotyping of Echinococcus. Int J Parasitol, 28, 1775-1779.
  • 13. Guo ZH, Kubo M, Kudo M ve ark. (2011): Growth and genotypes of Echinococcus granulosus found in cattle imported from Australia and fattened in Japan. Parasitol Int, 60, 498-502.
  • 14. McManus DP (2013): Current status of the genetics and molecular taxonomy of Echinococcus species. Parasitol, 140, 1617-1623.
  • 15. Nakao M, Lavikainen A, Yanagida T ve ark. (2013): Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). Int J Parasitol, 43, 1017-1029.
  • 16. Rojas CAA, Romig T, Lightowlers MW (2014): Echinococcus granulosus sensu lato genotypes infecting humans – review of current knowledge. Int J Parasitol, 44, 9-18.
  • 17. Simsek S, Balkaya I, Koroglu E (2010): Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet Parasitol, 172, 347-349.
  • 18. Simsek S, Cevik A (2014): First detection and molecular characterization of Echinococcus equinus in a mule in Turkey. Acta Parasitol, 59, 773-777.
  • 19. Simsek S, Eroksuz Y (2009): Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop, 109, 167- 169.
  • 20. Simsek S, Koroglu E, Dumanli N ve ark. (2005): Seroprevalance of cattle hydatidosis in some districts in the east Anatolian region of Turkey. Turk J Vet Anim Sci, 29, 1305-1310.
  • 21. Simsek S, Ozcetin C, Balkaya I (2012): Detection of polymorphism in AgB1 gene from sheep, cattle and human isolates of echinococcus granulosus by SSCP. Vet Parasitol, 184, 352-355.
  • 22. Snabel V, Altintas N, D'Amelio S ve ark. (2009): Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res, 105, 145-154.
  • 23. Thompson RCA (1998): Species and strains in the genus Echinococcus epidemiological and evoluationary perspectives. Parasit Int, 47, 133-281.
  • 24. Thompson RCA, Lymbery AJ (1995): Echinococcus and Hydatid Disease. CAB Internationa, UK.
  • 25. Thompson RCA, McManus DP (2002): Towards a taxonomic revision of the genus Echinococcus. Trends Parasit, 18, 452-457.
  • 26. Utuk AE, Piskin FC, Dalkilic B (2012): Molecular characterization of sheep isolates of Echinococcus granulosus in Kilis Province. Kafkas Univ Vet Fak Derg, 18, 35-38.
  • 27. Utuk AE, Simsek S (2013): Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey. J Helminthol, 87, 305-308.
  • 28. Utuk AE, Simsek S, Koroglu E ve ark. (2008): Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop, 107, 192-194.
  • 29. Ütük AE, Pişkin FÇ (2010): Melez bir dağ keçisinde hydatidosis ve moleküler karakterizasyonu. Kafkas Üniv Vet Fak Derg, 16, 671-673.
  • 30. Vural G, Baca AU, Gauci CG ve ark. (2008): Variability in the Echinococcus granulosus cytochrome c oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol, 154, 347- 350.
  • 31. Yıldız K, Gürcan S (2003): Prevalence of hydatidosis and fertility of hydatid cysts in sheep in Kirikkale, Turkey. Acta Vet Hung, 51, 181-187.
  • 32. Yıldız K, Gürcan S (2009): Differantiation of Echinococcus granulosus strain using larval rostellar hook morphometry in Turkey. Türkiye Parazitol Derg, 33, 199-202.
  • 33. Yıldız K, Tunçer Ç (2005): Kırıkkale’de sığırlarda kist hidatik’in yayılışı. Türkiye Parazitol Derg, 29, 247-250

Kırıkkale’de kesilen sığırlarda Echinococcus granulosus’un moleküler olarak genotiplendirilmesi

Year 2017, Volume: 64 Issue: 1, 51 - 54, 01.03.2017

Sami Gökpınar Rukiye Değirmenci Kader Yıldız

https://doi.org/10.1501/Vetfak_0000002773
Cited By: 2

Abstract

Echinococcus granulosus türü içindeki suş farklılıkları etkenin konak spesifitesi, gelişim hızı, patojenitesi, antijenik özellikleri, kemoterapotiklere duyarlılığı, bulaşma dinamikleri ve epidemiyolojide farklılıklar şekillendirmektedir. Bu çalışmada; Kırıkkale Yöresi’nde kesilen sığırların karaciğer ve akciğerlerinden elde edilen E. granulosus izolatlarının suş identifikasyonu yapılması amaçlanmıştır. Bunun için parazitin 12S rRNA ve mitokondriyal cytochrome c oxidase subunit 1 (COI) geni spesifik primerlerle çoğaltılmıştır ve COI geni sekanslatılmıştır. COI sekans sonucuna göre, elde edilen izolatların G1-G3 kompleksinde olduğu belirlenmiştir

Keywords

Echinococcus granulosus genotiplendirme hidatid kist Kırıkkale sığır

References

  • 1. Altintas N, Oztatlici M, Altintas N ve ark. (2013): Molecular analysis of cattle isolates of Echinococcus granulosus in Manisa Province of Turkey. Kafkas Univ Vet Fak Derg, 19, 455-459.
  • 2. Beyhan YE, Umur S (2011): Molecular characterization and prevalence of cystic echinococcosis in slaughtered water buffaloes in Turkey. Vet Parasitol, 181, 174-179.
  • 3. Bowles J, Blair D, McManus DP (1992): Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol, 54, 165-173.
  • 4. Bowles J, McManus DP (1993): NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol, 23, 969-972.
  • 5. Budak A, Yıldız K, Çakır Arıca Ş ve ark. (2010): Kırıkkale yöresinde koyunlardan elde edilen Echinococcus granulosus izolatlarının moleküler karakteri. Kafkas Üniv Vet Fak Derg, 16, 245-250.
  • 6. Cardona GA, Carmena D (2013): A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Vet Parasitol, 192, 10-32.
  • 7. Dinkel A, Njoroge EM, Zimmermann A ve ark. (2004): A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol, 34, 645-653.
  • 8. Eckert J, Gemmel MA, Meslin FX ve ark. (2001): WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Organisation of Animal Health, Paris, France.
  • 9. Eckert J, Thompson RCA (1997): Intraspesific variation of Echinococcus granulosus and related species with emphises on their infectivity to humans. Acta Trop, 64, 19- 34.
  • 10. Ergin S, Saribas S, Yuksel P ve ark. (2010): Genotypic characterisation of Echinococcus granulosus isolated from human in Turkey. Afr J Microbiol Res, 4, 551-555.
  • 11. Eryildiz C, Sakru N (2012): Molecular characterization of human and animal isolates of Echinococcus granulosus in the Thrace Region, Turkey. Balkan Med J, 29, 261-267.
  • 12. Gasser RB, Zhu X, McManus DP (1998): Dideoxy fingerprinting: Application to the genotyping of Echinococcus. Int J Parasitol, 28, 1775-1779.
  • 13. Guo ZH, Kubo M, Kudo M ve ark. (2011): Growth and genotypes of Echinococcus granulosus found in cattle imported from Australia and fattened in Japan. Parasitol Int, 60, 498-502.
  • 14. McManus DP (2013): Current status of the genetics and molecular taxonomy of Echinococcus species. Parasitol, 140, 1617-1623.
  • 15. Nakao M, Lavikainen A, Yanagida T ve ark. (2013): Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). Int J Parasitol, 43, 1017-1029.
  • 16. Rojas CAA, Romig T, Lightowlers MW (2014): Echinococcus granulosus sensu lato genotypes infecting humans – review of current knowledge. Int J Parasitol, 44, 9-18.
  • 17. Simsek S, Balkaya I, Koroglu E (2010): Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet Parasitol, 172, 347-349.
  • 18. Simsek S, Cevik A (2014): First detection and molecular characterization of Echinococcus equinus in a mule in Turkey. Acta Parasitol, 59, 773-777.
  • 19. Simsek S, Eroksuz Y (2009): Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop, 109, 167- 169.
  • 20. Simsek S, Koroglu E, Dumanli N ve ark. (2005): Seroprevalance of cattle hydatidosis in some districts in the east Anatolian region of Turkey. Turk J Vet Anim Sci, 29, 1305-1310.
  • 21. Simsek S, Ozcetin C, Balkaya I (2012): Detection of polymorphism in AgB1 gene from sheep, cattle and human isolates of echinococcus granulosus by SSCP. Vet Parasitol, 184, 352-355.
  • 22. Snabel V, Altintas N, D'Amelio S ve ark. (2009): Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res, 105, 145-154.
  • 23. Thompson RCA (1998): Species and strains in the genus Echinococcus epidemiological and evoluationary perspectives. Parasit Int, 47, 133-281.
  • 24. Thompson RCA, Lymbery AJ (1995): Echinococcus and Hydatid Disease. CAB Internationa, UK.
  • 25. Thompson RCA, McManus DP (2002): Towards a taxonomic revision of the genus Echinococcus. Trends Parasit, 18, 452-457.
  • 26. Utuk AE, Piskin FC, Dalkilic B (2012): Molecular characterization of sheep isolates of Echinococcus granulosus in Kilis Province. Kafkas Univ Vet Fak Derg, 18, 35-38.
  • 27. Utuk AE, Simsek S (2013): Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey. J Helminthol, 87, 305-308.
  • 28. Utuk AE, Simsek S, Koroglu E ve ark. (2008): Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop, 107, 192-194.
  • 29. Ütük AE, Pişkin FÇ (2010): Melez bir dağ keçisinde hydatidosis ve moleküler karakterizasyonu. Kafkas Üniv Vet Fak Derg, 16, 671-673.
  • 30. Vural G, Baca AU, Gauci CG ve ark. (2008): Variability in the Echinococcus granulosus cytochrome c oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol, 154, 347- 350.
  • 31. Yıldız K, Gürcan S (2003): Prevalence of hydatidosis and fertility of hydatid cysts in sheep in Kirikkale, Turkey. Acta Vet Hung, 51, 181-187.
  • 32. Yıldız K, Gürcan S (2009): Differantiation of Echinococcus granulosus strain using larval rostellar hook morphometry in Turkey. Türkiye Parazitol Derg, 33, 199-202.
  • 33. Yıldız K, Tunçer Ç (2005): Kırıkkale’de sığırlarda kist hidatik’in yayılışı. Türkiye Parazitol Derg, 29, 247-250
There are 33 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA55FC25HG
Journal Section Research Article
Authors

Sami Gökpınar

Rukiye Değirmenci

Kader Yıldız

Publication Date March 1, 2017
Published in Issue Year 2017Volume: 64 Issue: 1

Cite

APA Gökpınar, S., Değirmenci, R., & Yıldız, K. (2017). Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(1), 51-54. https://doi.org/10.1501/Vetfak_0000002773
AMA Gökpınar S, Değirmenci R, Yıldız K. Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Univ Vet Fak Derg. March 2017;64(1):51-54. doi:10.1501/Vetfak_0000002773
Chicago Gökpınar, Sami, Rukiye Değirmenci, and Kader Yıldız. “Genotyping of Echinococcus Granulosus Obtained from Cattle Slaughtered in Kırıkkale Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64, no. 1 (March 2017): 51-54. https://doi.org/10.1501/Vetfak_0000002773.
EndNote Gökpınar S, Değirmenci R, Yıldız K (March 1, 2017) Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 1 51–54.
IEEE S. Gökpınar, R. Değirmenci, and K. Yıldız, “Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province”, Ankara Univ Vet Fak Derg, vol. 64, no. 1, pp. 51–54, 2017, doi: 10.1501/Vetfak_0000002773.
ISNAD Gökpınar, Sami et al. “Genotyping of Echinococcus Granulosus Obtained from Cattle Slaughtered in Kırıkkale Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64/1 (March 2017), 51-54. https://doi.org/10.1501/Vetfak_0000002773.
JAMA Gökpınar S, Değirmenci R, Yıldız K. Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Univ Vet Fak Derg. 2017;64:51–54.
MLA Gökpınar, Sami et al. “Genotyping of Echinococcus Granulosus Obtained from Cattle Slaughtered in Kırıkkale Province”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 64, no. 1, 2017, pp. 51-54, doi:10.1501/Vetfak_0000002773.
Vancouver Gökpınar S, Değirmenci R, Yıldız K. Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Univ Vet Fak Derg. 2017;64(1):51-4.

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