Letter to Editor
Investigation of antimicrobial resistance and extended spectrum beta- lactamase production inEscherichia coli isolates of equine feces origin
Abstract
In this study, it was aimed to investigate the existence of ESBL and determine the resistance against various antibiotics of E. coli strains isolated from feces examples obtained from racing and jumping horses. One hundred E. coli isolates (racing: 37, jumping: 63) were analysed with 16 antibiotics by using the disc diffusion method and ESBL existence by fenotypic confirmatory test. The antibiotic resistance prevalences were compared between racing and jumping groups of horses. The highest resistance was obtained against tetracycline in both groups with 81.1% (30) in racing horses and 20.6% (13) in jumping horses. ESBL production has been determined in only 6 isolates among 100 E. coli isolates and all 6 ESBL positive isolates were isolated from the racing horses. In conclusion, the resistance prevalences to various antibiotics in racing horses were higher than jumping horses and ESBL production in the isolates from jumping horses was determined. The contamination of these agents in ecosystem could cause a potential risk factor for public health
Keywords
References
- Abbott I, Cerqueira GM, Bhuiyan S, et al. (2013):
- Carbapenem resistance in Acinetobacter baumannii, laboratory challenges, mechanistic insights and therapeutic strategies. Expert Rev Anti Infect Ther, 11, 395-409.
- Ahmed OM, Clegg PD, Williams NJ, et al. (2010): Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England, Ann Clin Microbiol Antimicrob, 9:12 http://www.ann-clinmicrob.com/content/9/1/12 (1 Ocak 2012)
- Aksoy A, Göçmen JS, Kaçmaz B ve ark. (2005): İnsan ve sığırlardan izole edilen fekal Escherichia coli suşların- da antibiyotik direnci ve genişlemiş spektrumlu beta- laktamaz üretimi. ANKEM Derg, 19, 130-134.
- Arda M, Minbay A, Leloğlu N ve ark. (1999): Fakültatif anerobik gram negatif çomaklar, Enterobacteriaceae familyası. 45-47. In: Özel Mikrobiyoloji, 4.baskı Medisan Ltd. Şti. Ankara.
- Bauernfeind A, Horl G (1987): Novel R-factor-borne beta-laktamaz conferring resistance to cephalosporins. Infect. 15, 257-259.
- Bradford PA (2001): Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev, 14, 933-951.
- Bryan J, Leonard N, Fanning S, et al. (2010): Antimicrobial resistance in commensal faecals Escherichia coli of hospitalised horses. Irish Vet J, 63, 373-379.
- Bush K, Jacoby GA, Medeiros AA (1995): A functional classification scheme for betalactamases and its correlation with molecular structure. Antimicrob Agents Chemother, 39, 1211-1233.
- CLSI (2007): Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing. 17th informational supplement. approvedstandard, MS100-S17, Wayne, PA, USA.
- Dinç G, Ata Z, Temelli S (2012): Sığır mastitislerinden izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz aktivitesi ve antibiyotik dirençli- lik profilinin incelenmesi. Ankara Üniv Vet Fak Derg, 59, 85-88.
- Dolejska M, Jurcickova Z, Literak I, et al. (2011): IncN plasmids carrying blaCTX-M-1 in Escherichia coli isolates on a dairy Farm. Vet Microbiol, 149, 513-516.
- Dolejska M, Duskova E, Rybarikova J, et al. (2011): Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from an equine clinic and a hor- seback riding centre. J Antimicrob Chemother, 66, 757- 764.
- Dunoswska M, Morley PS, Traub-Dargatz JL, et al. (2006): Impact of hospitalizasyon and antimicrobial drug adminstreation on antimicrobial susceptibilitiy patterns of commensal Escherichia coli isolated from the feces of horses. JAVMA, 228, 1909-1917.
- Emery CL, Weymouth LA (1997): Detection and clinical signficance ofextended spectrum βeta-lactamases in a tertiary-care medical center. J Clini Microbiol, 35, 2061- 2067.
- Endtz HP, Dijk WC, Verbrugh HA, et al. (1997): Comparative in vitro activitiy of meropenem against selected pathogens from hospitalized patients in the Netherlans. MASTIN Study Group. J Antimic Chemother, 39, 149-56.
- Gür D (2000): Hastane infeksiyonları ve antimikrobiyal ajanlara çoğul dirençli gram negatif bakteriler. Hast İnfek Derg, 4, 218-25.
- Harada K ve Asai T (2010): Role of antimicrobial selective pressure and secondary factors on antimicrobial resistance prevalence in Escherichia coli from food- producing animals in japan hindawi publishing corpora- tion. doi:10.1155/2010/180682. Article ID 180682,
- Jacoby GA ve Munoz-Price LS (2005): The new beta-lactamases. N Engl J Med, 352, 380-391.
- Johns I, Verheyen K, Good L, et al. (2012): Antimicrobial resistance in faecal Escherichia coli isolates from horses treated with antimicrobials: A longitudinal study in hospitalised and non-hospitalised horses. Vet Mic, 12, 381-389.
- Livermore DM, Canton R, Gniadkowski, et al. (2007): CTX-M: Changing the face of ESBLs in Europe. J Antimic Chemother, 59, 165-74.
- Matsumoto Y, Ikeda F, Kamimura T, et al. (1988): Novel plasmid-mediated beta-lactamase from Escherichia coli that inactivates oxyimino-cephalosporins. Antimicrob Agents Chemother, 32, 1243-1246.
- Pitout JDD, Nordmann P, Laupland KB, et al. (2005): Emergence extended-spectrum β-lactamases (ESBLs) in the commu- nity. J Antimicrob Chemother, 56, 52-59. producing
- Quinn PJ, Markey BK, Leonard FC, et al. (2011): Antimicrobial Resistance In: Veterinary Microbiology and Microbial Disease, 157, Second Edition, Blackwell Science Ltd, Iowa, USA.
- Stürenberg E ve Mack D (2003): Extended-spectrum beta-lactamases: Implications for the clinical microbio- logy laboratory, therapy, and infection control. J Infect 47, 273-295.
- Weldhagen GF (2004): Integrons and beta-lactamases a novel perspective on resistance. Int J Antimicrob Agents, 23, 556-62.
- Yorgancıgil B (1999): Beta laktam antibiyotiklere karşı oluşan direnç mekanizmaları. Turgut Özal Tıp Merkezi Derg, 6, 176-82.
At fekal orijinli Escherichia coli izolatlarında antimikrobiyal direnç vegenişlemiş spektrumlu beta laktamaz üretiminin araştırılması
Abstract
Bu çalışma, düz yarış ve konkur atlarından alınan fekal örneklerden izole edilen Escherichia coli izolatlarının çeşitli antibiyotiklere direnç durumlarını belirlemek ve genişlemiş spektrumlu beta laktamaz (GSBL) varlığını araştırmak amacıyla yapıldı. Çalışmada izole edilen 100 E. coli (düz yarış: 37, konkur: 63) izolatının 16 antibiyotiğe karşı duyarlılıkları disk difüzyon yöntemiyle, GSBL varlığı ise fenotipik doğrulama testi ile belirlendi. Düz yarış ve konkur grupları arasında antibiyotik direnç oranları karşılaştırıldı. En yüksek antibiyotik direnci, düz yarış atlarında %81.1 (30/37) ve konkur atlarında %20.6 (13/63) oranlarında olmak üzere tetrasikline karşı belirlendi. Atlardan izole edilen 100 E. coli izolatının ise 6 (%6) tanesinde GSBL üretimi fenotipik olarak doğrulandı. GSBL pozitif izolatların tümü düz yarış atlarından izole edildi. Sonuç olarak, düz yarış atlarının fekal florasından elde edilen E. coli izolatlarında çeşitli antibiyotiklere direnç daha yüksek oldu ve bu izolatlarda GSBL üretimi belirlendi. Bu etkenlerin ekosisteme bulaşması halk sağlığı açısından potansiyel bir risk oluşturabilir
References
- Abbott I, Cerqueira GM, Bhuiyan S, et al. (2013):
- Carbapenem resistance in Acinetobacter baumannii, laboratory challenges, mechanistic insights and therapeutic strategies. Expert Rev Anti Infect Ther, 11, 395-409.
- Ahmed OM, Clegg PD, Williams NJ, et al. (2010): Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England, Ann Clin Microbiol Antimicrob, 9:12 http://www.ann-clinmicrob.com/content/9/1/12 (1 Ocak 2012)
- Aksoy A, Göçmen JS, Kaçmaz B ve ark. (2005): İnsan ve sığırlardan izole edilen fekal Escherichia coli suşların- da antibiyotik direnci ve genişlemiş spektrumlu beta- laktamaz üretimi. ANKEM Derg, 19, 130-134.
- Arda M, Minbay A, Leloğlu N ve ark. (1999): Fakültatif anerobik gram negatif çomaklar, Enterobacteriaceae familyası. 45-47. In: Özel Mikrobiyoloji, 4.baskı Medisan Ltd. Şti. Ankara.
- Bauernfeind A, Horl G (1987): Novel R-factor-borne beta-laktamaz conferring resistance to cephalosporins. Infect. 15, 257-259.
- Bradford PA (2001): Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev, 14, 933-951.
- Bryan J, Leonard N, Fanning S, et al. (2010): Antimicrobial resistance in commensal faecals Escherichia coli of hospitalised horses. Irish Vet J, 63, 373-379.
- Bush K, Jacoby GA, Medeiros AA (1995): A functional classification scheme for betalactamases and its correlation with molecular structure. Antimicrob Agents Chemother, 39, 1211-1233.
- CLSI (2007): Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing. 17th informational supplement. approvedstandard, MS100-S17, Wayne, PA, USA.
- Dinç G, Ata Z, Temelli S (2012): Sığır mastitislerinden izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz aktivitesi ve antibiyotik dirençli- lik profilinin incelenmesi. Ankara Üniv Vet Fak Derg, 59, 85-88.
- Dolejska M, Jurcickova Z, Literak I, et al. (2011): IncN plasmids carrying blaCTX-M-1 in Escherichia coli isolates on a dairy Farm. Vet Microbiol, 149, 513-516.
- Dolejska M, Duskova E, Rybarikova J, et al. (2011): Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from an equine clinic and a hor- seback riding centre. J Antimicrob Chemother, 66, 757- 764.
- Dunoswska M, Morley PS, Traub-Dargatz JL, et al. (2006): Impact of hospitalizasyon and antimicrobial drug adminstreation on antimicrobial susceptibilitiy patterns of commensal Escherichia coli isolated from the feces of horses. JAVMA, 228, 1909-1917.
- Emery CL, Weymouth LA (1997): Detection and clinical signficance ofextended spectrum βeta-lactamases in a tertiary-care medical center. J Clini Microbiol, 35, 2061- 2067.
- Endtz HP, Dijk WC, Verbrugh HA, et al. (1997): Comparative in vitro activitiy of meropenem against selected pathogens from hospitalized patients in the Netherlans. MASTIN Study Group. J Antimic Chemother, 39, 149-56.
- Gür D (2000): Hastane infeksiyonları ve antimikrobiyal ajanlara çoğul dirençli gram negatif bakteriler. Hast İnfek Derg, 4, 218-25.
- Harada K ve Asai T (2010): Role of antimicrobial selective pressure and secondary factors on antimicrobial resistance prevalence in Escherichia coli from food- producing animals in japan hindawi publishing corpora- tion. doi:10.1155/2010/180682. Article ID 180682,
- Jacoby GA ve Munoz-Price LS (2005): The new beta-lactamases. N Engl J Med, 352, 380-391.
- Johns I, Verheyen K, Good L, et al. (2012): Antimicrobial resistance in faecal Escherichia coli isolates from horses treated with antimicrobials: A longitudinal study in hospitalised and non-hospitalised horses. Vet Mic, 12, 381-389.
- Livermore DM, Canton R, Gniadkowski, et al. (2007): CTX-M: Changing the face of ESBLs in Europe. J Antimic Chemother, 59, 165-74.
- Matsumoto Y, Ikeda F, Kamimura T, et al. (1988): Novel plasmid-mediated beta-lactamase from Escherichia coli that inactivates oxyimino-cephalosporins. Antimicrob Agents Chemother, 32, 1243-1246.
- Pitout JDD, Nordmann P, Laupland KB, et al. (2005): Emergence extended-spectrum β-lactamases (ESBLs) in the commu- nity. J Antimicrob Chemother, 56, 52-59. producing
- Quinn PJ, Markey BK, Leonard FC, et al. (2011): Antimicrobial Resistance In: Veterinary Microbiology and Microbial Disease, 157, Second Edition, Blackwell Science Ltd, Iowa, USA.
- Stürenberg E ve Mack D (2003): Extended-spectrum beta-lactamases: Implications for the clinical microbio- logy laboratory, therapy, and infection control. J Infect 47, 273-295.
- Weldhagen GF (2004): Integrons and beta-lactamases a novel perspective on resistance. Int J Antimicrob Agents, 23, 556-62.
- Yorgancıgil B (1999): Beta laktam antibiyotiklere karşı oluşan direnç mekanizmaları. Turgut Özal Tıp Merkezi Derg, 6, 176-82.
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA29CF57KP |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2016 |
| Published in Issue | Year 2016Volume: 63 Issue: 4 |