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Florozisin koyunlarda bazı mineraller ve hormonlar üzerine etkilerinin araştırılması

Year 2016, Volume: 63 Issue: 3, 223 - 227, 01.09.2016

Bahat Comba Ali Çınar

Abstract

Bu çalışmada; florozisin koyunlarda plazma flor (F), serum kalsiyum (Ca), fosfor (P), magnezyum (Mg), kalsitonin (CT), intakt paratiroid hormon (iPTH) ve vitamin D3 (Vit D3) üzerine etkileri araştırıldı. Araştırmada 3-4 yaşlarında 50 tanesi Tendürek Dağı (yaklaşık 2000 m) bölgesinde kronik florozisli (Florozisli grup) ve 25 tanesi Van bölgesinden (1700 m) florozis belirtisi göstermeyen (Kontrol grubu) toplamda 75 Morkaraman Koyunu kullanıldı. Türkiye’ nin Doğu Anadolu bölgesindeki VanAğrı şehirlerinden kronik florozisli 50 koyundan toplanan veriler, Van bölgesinde florozis belirtisi göstermeyen 25 koyunla karşılaştırıldı. Plazma F seviyeleri spesifik flor elektrodu ile ölçüldü, serum Ca, P ve Mg seviyeleri fotometrik yolla, serum CT ve iPTH seviyeleri immünometrik kemilüminesans assay yöntemi ile belirlendi, serum Vit D3 seviyesi HPLC metodu ile analiz edildi. Florozisli gruptaki serum P ve Vit D3 seviyeleri düşük (p<0.001), serum CT (p<0.01) and iPTH (p<0.001) düzeyleri kontrol grubundan yüksekti. Ca ve Mg değerleri istatistiksel olarak önemsizdi. Elde edilen bulgular, uzun süreli ve yüksek miktarda flora maruz kalma sonucunda koyunlarda osteomalatik durumun oluşabileceğini desteklemektedir. Sonuç olarak, florozis şekillendikten sonra dişlerde ve iskelet sisteminde dönüşü olmayan patolojik bir durum söz konusu olduğundan, bu bölgede yaşayanlara temiz su kaynakları verilmeli. Aksi durumda florun dişler ve kemikler üzerindeki olumsuz etkisini önlemek için uygun dozlarda Ca, P, Mg ve Vit D3 ilavesi yapılmalıdır

Keywords

Florozis kalsiyum koyun paratiroid hormon Vitamin D

References

  • Altıntaş A, Fidancı UR, Sel T, et al. (2000): Serum proteins electrophoresis and kidney function in sheep with natural and industrial fluorosis. Vet J Ankara Univ, 2, 105-114.
  • Arslan S (2008): The relationship between thyroid hormones and fluoride levels in goats, with fluorosis, University of Yuzuncu Yil, Institute of Health Science Department of Internal Diseases, PhD Thesis, Van, Turkey.
  • Başaran B, Kaya N (2004): Deneysel kronik florozis oluşturulmuş tuj ırkı koyunlarda serum paratiroid hormon (PTH) ve kalsitonin (CT) tayini. XVIII. Ulusal Kimya Kongresi, Kars.
  • Blood DC, Radostits OM, Henderson JA (1983): Fluorine Poisoning. Veterinary Medicine, Sixth Edition, London.
  • Çınar A, Selçuk M (2005): Effects of chronic fluorosis on thyroxine, triiodothyronine, and proteın-bound iodıne in cows. Fluoride, 1, 65-68.
  • Doğan İ (2002): Investigation of antioxidant compounds of fluorotic sheep, Yuzuncu Yil University, Health Science Institute, Department of Biochemistry MSci. Thesis, Van, Turkey.
  • Dönmez N, Çınar A (2003): Effects of chronic fluorosis on electrocardiogram in sheep. Biol Trace Elem Res, 2, 115-122.
  • Duly EB, Luney SR, Trinick TR, et al. (1995): Validation of an ion selective electrode system for the analysis of serum fluoride ion. J Automat Chem, 6, 219-223.
  • Faccini JM, Teoita SPS (1974): Histopathological assessment of endemic skeletal fluorosis. Calcified Tissue Res, 1, 45-47.
  • Greenberg SR (1986): Response of the renal supporting tissues to chronic fluoride exposure as revealed by a special technique. Urol Int, 41, 91-94.
  • Gupta SK, Khan TI, Gupta RC (2001): Compensatory hyperparathyroidsm following high fluoride ingestion biochemical correlation. Indian Pediatr, 38,139-146.
  • Guyton AC (2007): Textbook of Medical Physiology. 11th ed. Philadelphia: W.B. Saunders Company.
  • Huang Z, Li K, Hou G, et al. (2002): Study on the correlation of the biochemical indexes in fluoride workers. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 3, 192-194.
  • Junxiang M, Mingfeng L, Yu'e S, et al. (2009): Serum osteocalcin and calcitonin in adult males with different fluoride exposures. Fluoride, 2, 133-136.
  • Khandare AL, Harikumar R, Sivakumar B (2005): Severe bone deformities in young children from vitamin d deficiency and fluorosis in Bihar-India. Calcif Tissue Int, 76, 412-418.
  • Kılıçalp D, Çınar A, Belge F (2004): Effects of chronic fluorosis on electrocardiogram in dogs. Fluoride, 2, 96- 100.
  • Kırvar E (1991): Serum levels of calcium, phosphorus, sodium, potassium, urea and uric acid in normal sheep and sheep showing signs of fluorosis in Eastern Anatolia. Ankara University Health Science Institute Department of Biochemistry PhD Thesis, Ankara, Turkey.
  • Kruse K (1995): Endocrine control of calcium and bone metabolism, 712-743. In: Clinical Paediatric Endocrinology, 3th ed, Backwell Science Ltd, Oxford.
  • Lantz O, Jouvin MH, De Vernejoul MC, et al. (1987): Fluoride-induced chronic renal failure. Am J Kidney Dis, 10, 136-139.
  • Maraşlı Ş, Maraşlı N, Yenigün A (1995): The preliminary report on hypocupraemia in sheep enzootic fluorosis. Kafkas Univ Vet Fak Derg, 1-2, 79-81.
  • Mittal RL, Sidhu SS, Khokhar SS (1987): Role of copper in skelatal changes in fluorosis, An experimental study in rabbits. Fluoride, 3, 104-108.
  • Mjor IA (2002): Pulp-dentin biology in restorative dentistry, China. Quintessence Publishing Co, Inc.
  • Morton S, Roberts DJ (1993): University of Bristol Unicam AAS Methods, Manual Issue, 2 (05/93), England.
  • Oto G (2002): The effect of seasonal changes on fluor levels in water and blood samples taken from sheep living in the region of Muradiye and Caldiran. University of Yuzuncu Yıl Health Sciences Institute, Ms. Sci. Thesis in Pharmacology and Toxicology, Van, Turkey.
  • Patra RC, Dwivedi SK, Bhardwaj B, et al. (2000): Industrial fluorosis in cattle and buffalo around Udaipur, India. Sci Total Environ, 253, 145-150.
  • Reynolds SL, Judd HJ (1984): Rapid procedure for the determination of vitamins a and d in fortified skimmed milk powder using high-performance liquid chromatography, Analyst, 109, 489-492.
  • Sharifian A, Momeni Vr, Shahriaran S, et al. (2008): Serum calcium and parathyroid hormone levels in alumınum potroom workers exposed to fluorıde emissions. Fluoride, 4, 314-316.
  • Shi SX, Zhu ZG, Suo R, et al. (2003): Rapid method for the determination of trace fluoride and activation of ion- selective. Electrode Analytical Sci, 19, 671-673.
  • Shills ME, Olson JA, Shike M, et al. (1999): Modern Nutrition in Health and Disease, 9th edition, Baltimore, Williams & Williams.
  • Singh PP, Barjatiya MK, Dhing S, et al. (2001): Evidence suggesting that high intake of fluoride provokes nephrolithiasis in tribal populations. Urol Res, 4, 238- 244.
  • Tokar VI, Voroshnin VV, Sherbakov SV (1989): Chronic effects of fluorides on the pituitary-thyroid system in industrial workers in Russian. Gig Tr Prof Zabol, 9, 19- 22.
  • Yaşar S (2003): Investigation of the vitamins and mineral levels in sheep with fluorosis. Yuzuncu Yil University, Health Science Institute, Department of Biochemistry MSci. Thesis, Van, Turkey. Geliş tarihi: 13.01.2015 / Kabul tarihi: 11.08.2015 Address for correspondence: Yrd. Doç. Dr. Bahat COMBA, Yuzuncu Yil University Faculty of Veterinary Medicine, Department of Phsiology, Postal code: 65080, Zeve Campus, Van, Turkey. e-mail: bahatcomba@hotmail.com.tr

Investigation of effects of fluorosis on some minerals and hormones in sheep

Year 2016, Volume: 63 Issue: 3, 223 - 227, 01.09.2016

Bahat Comba Ali Çınar

Abstract

This study was carried out to determine the effects of fluorosis on plasma fluor (F), serum calcium (Ca), phosphorus (P), magnesium (Mg), calcitonin (CT), intact parathyroid hormone (iPTH) and vitamin D3 (Vit D3) in sheep. A total of 75 Morkaraman sheep, 50 with chronic fluorosis (Fluorotic group) from Tendürek Mountain region (altitude about 2000 m) and 25 without any fluorosis (Control group) from Van (altitude 1700m) were used, aged 3-4 years. Data collected 50 sheeps with chronic fluorosis in the in East Anatolia, Van-Agri, Turkey, were compared with data from 25 without any fluorosis sheeps from the Van region. Plasma F levels were measured with spesific fluride electrode, serum Ca, P and Mg levels were photometrically determined; serum CT and iPTH levels were determined using chemiluminescent immunometric assay, serum Vit D3 level were analysed with HPLC method. Serum P and Vit D3 levels were lower in fluorotic group than in control group (p<0.001), while serum CT (p<0.01) and iPTH (p<0.001) levels were higher. The differences for Ca and Mg values were found statistically nonsignificant. The results obtained were after a long term exposure of sheep to high amounts of fluorine and supports occurrence of osteomalatic conditions. In conclusion, clean water should be given to animal and people who live in this region because of irreversible pathological situation occurs after fluorosis develops. Otherwise, Ca, P, Mg and Vit D3 supplementations has to be given at proper doses to prevent negative effects of fluoride on teeth and bones

Keywords

Calcium fluorosis parathyroid hormone sheep Vitamin D

References

  • Altıntaş A, Fidancı UR, Sel T, et al. (2000): Serum proteins electrophoresis and kidney function in sheep with natural and industrial fluorosis. Vet J Ankara Univ, 2, 105-114.
  • Arslan S (2008): The relationship between thyroid hormones and fluoride levels in goats, with fluorosis, University of Yuzuncu Yil, Institute of Health Science Department of Internal Diseases, PhD Thesis, Van, Turkey.
  • Başaran B, Kaya N (2004): Deneysel kronik florozis oluşturulmuş tuj ırkı koyunlarda serum paratiroid hormon (PTH) ve kalsitonin (CT) tayini. XVIII. Ulusal Kimya Kongresi, Kars.
  • Blood DC, Radostits OM, Henderson JA (1983): Fluorine Poisoning. Veterinary Medicine, Sixth Edition, London.
  • Çınar A, Selçuk M (2005): Effects of chronic fluorosis on thyroxine, triiodothyronine, and proteın-bound iodıne in cows. Fluoride, 1, 65-68.
  • Doğan İ (2002): Investigation of antioxidant compounds of fluorotic sheep, Yuzuncu Yil University, Health Science Institute, Department of Biochemistry MSci. Thesis, Van, Turkey.
  • Dönmez N, Çınar A (2003): Effects of chronic fluorosis on electrocardiogram in sheep. Biol Trace Elem Res, 2, 115-122.
  • Duly EB, Luney SR, Trinick TR, et al. (1995): Validation of an ion selective electrode system for the analysis of serum fluoride ion. J Automat Chem, 6, 219-223.
  • Faccini JM, Teoita SPS (1974): Histopathological assessment of endemic skeletal fluorosis. Calcified Tissue Res, 1, 45-47.
  • Greenberg SR (1986): Response of the renal supporting tissues to chronic fluoride exposure as revealed by a special technique. Urol Int, 41, 91-94.
  • Gupta SK, Khan TI, Gupta RC (2001): Compensatory hyperparathyroidsm following high fluoride ingestion biochemical correlation. Indian Pediatr, 38,139-146.
  • Guyton AC (2007): Textbook of Medical Physiology. 11th ed. Philadelphia: W.B. Saunders Company.
  • Huang Z, Li K, Hou G, et al. (2002): Study on the correlation of the biochemical indexes in fluoride workers. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 3, 192-194.
  • Junxiang M, Mingfeng L, Yu'e S, et al. (2009): Serum osteocalcin and calcitonin in adult males with different fluoride exposures. Fluoride, 2, 133-136.
  • Khandare AL, Harikumar R, Sivakumar B (2005): Severe bone deformities in young children from vitamin d deficiency and fluorosis in Bihar-India. Calcif Tissue Int, 76, 412-418.
  • Kılıçalp D, Çınar A, Belge F (2004): Effects of chronic fluorosis on electrocardiogram in dogs. Fluoride, 2, 96- 100.
  • Kırvar E (1991): Serum levels of calcium, phosphorus, sodium, potassium, urea and uric acid in normal sheep and sheep showing signs of fluorosis in Eastern Anatolia. Ankara University Health Science Institute Department of Biochemistry PhD Thesis, Ankara, Turkey.
  • Kruse K (1995): Endocrine control of calcium and bone metabolism, 712-743. In: Clinical Paediatric Endocrinology, 3th ed, Backwell Science Ltd, Oxford.
  • Lantz O, Jouvin MH, De Vernejoul MC, et al. (1987): Fluoride-induced chronic renal failure. Am J Kidney Dis, 10, 136-139.
  • Maraşlı Ş, Maraşlı N, Yenigün A (1995): The preliminary report on hypocupraemia in sheep enzootic fluorosis. Kafkas Univ Vet Fak Derg, 1-2, 79-81.
  • Mittal RL, Sidhu SS, Khokhar SS (1987): Role of copper in skelatal changes in fluorosis, An experimental study in rabbits. Fluoride, 3, 104-108.
  • Mjor IA (2002): Pulp-dentin biology in restorative dentistry, China. Quintessence Publishing Co, Inc.
  • Morton S, Roberts DJ (1993): University of Bristol Unicam AAS Methods, Manual Issue, 2 (05/93), England.
  • Oto G (2002): The effect of seasonal changes on fluor levels in water and blood samples taken from sheep living in the region of Muradiye and Caldiran. University of Yuzuncu Yıl Health Sciences Institute, Ms. Sci. Thesis in Pharmacology and Toxicology, Van, Turkey.
  • Patra RC, Dwivedi SK, Bhardwaj B, et al. (2000): Industrial fluorosis in cattle and buffalo around Udaipur, India. Sci Total Environ, 253, 145-150.
  • Reynolds SL, Judd HJ (1984): Rapid procedure for the determination of vitamins a and d in fortified skimmed milk powder using high-performance liquid chromatography, Analyst, 109, 489-492.
  • Sharifian A, Momeni Vr, Shahriaran S, et al. (2008): Serum calcium and parathyroid hormone levels in alumınum potroom workers exposed to fluorıde emissions. Fluoride, 4, 314-316.
  • Shi SX, Zhu ZG, Suo R, et al. (2003): Rapid method for the determination of trace fluoride and activation of ion- selective. Electrode Analytical Sci, 19, 671-673.
  • Shills ME, Olson JA, Shike M, et al. (1999): Modern Nutrition in Health and Disease, 9th edition, Baltimore, Williams & Williams.
  • Singh PP, Barjatiya MK, Dhing S, et al. (2001): Evidence suggesting that high intake of fluoride provokes nephrolithiasis in tribal populations. Urol Res, 4, 238- 244.
  • Tokar VI, Voroshnin VV, Sherbakov SV (1989): Chronic effects of fluorides on the pituitary-thyroid system in industrial workers in Russian. Gig Tr Prof Zabol, 9, 19- 22.
  • Yaşar S (2003): Investigation of the vitamins and mineral levels in sheep with fluorosis. Yuzuncu Yil University, Health Science Institute, Department of Biochemistry MSci. Thesis, Van, Turkey. Geliş tarihi: 13.01.2015 / Kabul tarihi: 11.08.2015 Address for correspondence: Yrd. Doç. Dr. Bahat COMBA, Yuzuncu Yil University Faculty of Veterinary Medicine, Department of Phsiology, Postal code: 65080, Zeve Campus, Van, Turkey. e-mail: bahatcomba@hotmail.com.tr
There are 32 citations in total.

Details

Other ID JA93UA24UF
Journal Section Research Article
Authors

Bahat Comba

Ali Çınar

Publication Date September 1, 2016
Published in Issue Year 2016Volume: 63 Issue: 3

Cite

APA Comba, B., & Çınar, A. (2016). Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 223-227.
AMA Comba B, Çınar A. Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Univ Vet Fak Derg. September 2016;63(3):223-227.
Chicago Comba, Bahat, and Ali Çınar. “Investigation of Effects of Fluorosis on Some Minerals and Hormones in Sheep”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 3 (September 2016): 223-27.
EndNote Comba B, Çınar A (September 1, 2016) Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 3 223–227.
IEEE B. Comba and A. Çınar, “Investigation of effects of fluorosis on some minerals and hormones in sheep”, Ankara Univ Vet Fak Derg, vol. 63, no. 3, pp. 223–227, 2016.
ISNAD Comba, Bahat - Çınar, Ali. “Investigation of Effects of Fluorosis on Some Minerals and Hormones in Sheep”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/3 (September 2016), 223-227.
JAMA Comba B, Çınar A. Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Univ Vet Fak Derg. 2016;63:223–227.
MLA Comba, Bahat and Ali Çınar. “Investigation of Effects of Fluorosis on Some Minerals and Hormones in Sheep”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 3, 2016, pp. 223-7.
Vancouver Comba B, Çınar A. Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Univ Vet Fak Derg. 2016;63(3):223-7.