Sıçanlarda ACTH uygulamasının böbrek fonksiyon testleri, elektrolitler ve hematolojik parametreler üzerine etkileri
Abstract
Bu çalışmada, sıçanlarda ACTH uygulamasının böbrek fonksiyon testlerine, elektrolitlere ve hematolojik parametreler üzerine etkilerini araştırmak amaçlandı. Araştırmada kontrol ve stres olmak üzere iki grup oluşturuldu. Her grupta 6’şar adet yaklaşık 13 haftalık, 200-250 g ağırlığında, Sprague Dawley ırkı erkek sıçan kullanıldı. Kontrol grubuna 0.5 ml serum izotonik/birey, stres grubuna ise 4 mg/kg dozunda ACTH intraperitoneal (i.p.) olarak bir kez uygulandı. Bu uygulamadan 3 saat sonra kan örnekleri alındı. Serumda glukoz, amilaz, kreatinin, kreatin kinaz (CK), kreatin kinaz MB (CK-MB), üre, ürik asit, kan üre azotu (BUN), laktat dehidrojenaz (LDH), kalsiyum (Ca), fosfor (P), magnezyum (Mg), sodyum (Na), klor (Cl), potasyum (K) analizleri, tam kanda ise total lökosit (WBC), eritrosit (RBC), hemoglobin (HGB), hematokrit (HCT), trombosit (PLT) ölçümleri yapıldı. Stres grubunda glukoz, WBC, % monosit ve % granülosit düzeyleri P≤0.001 önemde, CK, CK-MB, üre, BUN değerleri P≤0.05 önemde kontrollere göre yüksek bulunurken; % lenfosit (P≤0.001), Na, Cl, ve K (P≤0.05) seviyeleri ise düşük bulundu. Bu sonuçlar, ACTH ile oluşturulan stresin böbrek fonksiyon testleri, elektrolit dengesi ve hematolojik parametreler üzerine etkilerinin dikkate değer olduğunu gösterdi
Keywords
ACTH böbrek elektrolit glikoz hematolojik parametreler stres
References
- Agrawal S, Gupta D (2013): Assessment of liver damage in male Albino rats after repetitive heat stress of moderate level. Natl J Physiol Pharm Pharmacol, 3, 147-152.
- Anonim (2014): http://www.wikipedia.org/wiki/Dosya:Urea.png. Erişim Tarihi: Mayıs 2014.
- Aşcıoğlu YT (2005): Sıçanlardaki Kronik Alkolik Karaci- ğer Hasarına Likopenin Etkisi. Uzmanlık Tezi, İstanbul.
- Belge F, Çınar A, Selçuk M (2003): Effects of stress produced by adrenocorticotropin (ACTH) on lipid peroxidation and some antioxidants in vitamin C treated and nontreated chickens. S African J Anim Sci, 33, 201- 205.
- Cannon, WB (1935): Stresses and strains of homeostasis. Am J Med Sci, 189, 1-14.
- Cengiz F (2001): Hayvanlarda zorlanım (stres) oluşturan etkenler. J Fac Vet Med, 20, 147-153.
- Çınar A, Belge F, Dönmez N, et al. (2006): Effects of stress produced by adrenocorticotropin (ACTH) on ECG and some blood parameters in vitamin C treated and non-treated chickens. Veterinarski Arhiv, 76, 227- 235.
- Dönmez HH, Yörük M, Çınar A, Dönmez N (2007): Effects of vitamin C on ANAE positivity and blood cells in ACTH induced stress in chicken. Ind Vet J, 84, 1135- 1138.
- Dönmez N, Atalay B (2007): Sıcaklık stresi oluşturulan broylerlerde antibakteriyal etkili bitki ekstraktının (herbromix ®) bazı hematolojik parametreler üzerine etki- si. Ata Üniv Vet Bil Derg, 2, 82-86.
- Emre B, Sulu N, Hatipoğlu Ş, Çınar A (1994): C vitamini uygulanan ve uygulanmayan tavuklarda ACTH' nın glikoz ve insülin düzeylerine etkisi. Ankara Üniv Vet Fak Derg, 41, 10-17.
- Erdem E, Özdemir A, Kaya C, et al. (2012): Kronik böbrek yetmezliğinde serum ürik asit düzeyleri. Fırat Tıp Derg, 17, 23-27.
- Freeman BM (1975): Physiological basis of stres. Proc Roy Soc Med, 68, 427-429.
- Gölbaşı Z, Aydoğdu S, Sakallı M, et al. (1999): Kreatin kinaz-MB total kreatin kinazdan yüksek olabilir mi? Bir Olgu Sunusu. Türk Kardiol Dern Arş, 27, 515-517.
- Gray HG, Paradis TJ, Chang PW (1989): Research note: Physiological effects of adrenocorticotropic hormone and hydrocortisone in laying hens. Int J Poult Sci, 68, 1710-1713.
- Hall SJG, Broom DM, Goode JA, et al. (1999): Physiological responses of sheep during long road journeys involving ferry crossings. Anim Sci, 69, 19-27.
- Hill JA (1983): Indicators of stress in poultry. World’s Poult Sci J, 39, 24-32.
- Khan MR, Badar I, Siddiquah A (2011): Prevention of hepatorenal toxicity with Sonchusasper in gentamicin treated rats. BMC Complement Altern Med, 11,113.
- Matwichuk CL, Taylor SM, Shmon CL, et al. (1999): Changes in rectal temparature and hematologic, biochemical, blood gas, and acid-base values in healty Labrador Retrievers before and after strenuous exercise. Am J Vet Res, 1, 88-92.
- Mowbray BM, Bishop DM (1953): A.C.T.H. in diagnosis of adrenal insufficiency (Thorn Test). British Med J, 3, 17- 21.
- Nanda AS, Dobson H, Ward WR (1990): Relationship between an increase in plasma cortisol during transport- induced stress and failure of oestradiol to induce a luteinising hormone surge in dairy cows. Res Vet Sci, 49, 25-28.
- Öner AF, Arslan Ş, Özer R, et al. (1998): Steroid tedavi- si verilen hastalarda bazı biyokimyasal parametrelerin değerlendirilmesi. Van Tıp Derg, 5, 149-151.
- Pacak K, Palkovits M (2001): Stressor specificity of central neuroendocrine responses: implications for stress- related disorders. Endoc Rev, 22, 502-548.
- Polidori MC, Savino K, Alunni G, et al. (2002): Plasma lipophilic antioxidants and malondialdehyde in congestive heart failure patients: relationship to disease severity. Free Radic Biol Med, 15, 148-152.
- Puvadolpirod S, Thaxton JP (2000): Model of physiological stress in chickens 2. Dosimetry of adrenocorticotropi. Poult Sci, 79, 370-376.
- Reid IR, Grey AB (1993): Corticosteroid osteoporosis. Baillieres Clin Rheumatol 7, 573-587.
- Sapolsky RM, Romero LM, Munck, AU (2000): How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endoc Rev, 21, 55-89.
- Schneider PL, Beede DK, Wilcox CJ (1988): Nycterohemeral patterns of acid-base status, mineral concentrations and digestive function of lactating cows in natural or chamber heat stress environments. J Anim Sci, 66, 112-125.
- Siegel HS (1971): Adrenals, stress, and the environment. Worlds Poult Sci J, 27, 327-349.
- Simon J (1984): Effects of daily corticosterone injection upon plasma glicose, insulin, uric acid and electrolytes and food intake pattern in the chicken. Diabetes and Metabolism, 10, 211-217.
- Soliman KM, Abdul-Hamid M, Othman AI (2007): Effect of carnosine on gentamicin-induced nephrotoxicity. Med Sci Monit, 13, 73-83.
- Stevenson JR, Taylor R (1988): Effects of glucocrticoid and antiglucocorticoid hormones on leukocytes numbers and function. Int J Immunopharmacol, 10, 1-6.
- Topçu B (2009): Tavşanlarda ACTH ve Dekzametazon’un Oluşturduğu Kardiyovasküler Değişikliklere Karşı L- Karnitin’in Koruyucu Etkilerinin Araştırılması, Kafkas Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi, Kars.
- Ünal Ç (2010): Ratlarda Soğuk Stresinin Trioid Bezi Üze- rine Etkileri. Atatürk Üniversitesi, Sağlık Bilimleri Ensti- tüsü, Anatomi Anabilim Dalı, Erzurum.
- Zaidi SMKR, Al-Qirim TM, Banu N (2005): Effects of antioxidant vitamins on glutathione depletion and lipid peroxidation ınduced by restraint stress in the rat liver. Drugs R D, 6, 157-165. Geliş tarihi: 13.01.2015 / Kabul tarihi: 11.08.2015
Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats
Abstract
This study was conducted to investigate the effects of ACTH application on kidney function tests, electrolytes, and hematological parameters in rats. In the research two groups were formed, including control and stress. Each group consisted of 6 male Sprague Dawley rats which were about 13 weeks old and 200-250 g in weight. Serum isotonic was administered at a dose of 0.5 ml/rat to control group, and ACTH was applied at a dose of 4 mg/kg to stress group at once intraperitoneally. Blood samples were taken 3 hours after the application. Concentrations of glucose, amylase, creatine, creatine kinase (CK), creatine kinase MB (CK-MB), urea, uric acid, lactate dehydrogenase (LDH), calcium (Ca), phosphorus (P), magnesium (Mg) sodium (Na), chlorine (Cl) and potassium (K) in serum; the numbers of total leukocytes (WBC), platelet (PLT) and erythrocytes (RBC) and the values of hemoglobin (HGB) and hematocrit (HCT), on whole blood were performed. The level of glucose, WBC, monocyte %, granulocyte %, p≤0.001 values of stress group were significantly higher than those of control group (p≤0.001) while the values of CK, CK-MB, urea, BUN were significantly higher (p≤0.05) while lymphocytes % (p≤0.001) and the levels of Na, Cl, and K (p≤0.05) were significantly lower in stress group compared to control. These results indicated that stress induced by ACTH had noticeable impact on the renal function test, electrolyte balance and hematological parameters
References
- Agrawal S, Gupta D (2013): Assessment of liver damage in male Albino rats after repetitive heat stress of moderate level. Natl J Physiol Pharm Pharmacol, 3, 147-152.
- Anonim (2014): http://www.wikipedia.org/wiki/Dosya:Urea.png. Erişim Tarihi: Mayıs 2014.
- Aşcıoğlu YT (2005): Sıçanlardaki Kronik Alkolik Karaci- ğer Hasarına Likopenin Etkisi. Uzmanlık Tezi, İstanbul.
- Belge F, Çınar A, Selçuk M (2003): Effects of stress produced by adrenocorticotropin (ACTH) on lipid peroxidation and some antioxidants in vitamin C treated and nontreated chickens. S African J Anim Sci, 33, 201- 205.
- Cannon, WB (1935): Stresses and strains of homeostasis. Am J Med Sci, 189, 1-14.
- Cengiz F (2001): Hayvanlarda zorlanım (stres) oluşturan etkenler. J Fac Vet Med, 20, 147-153.
- Çınar A, Belge F, Dönmez N, et al. (2006): Effects of stress produced by adrenocorticotropin (ACTH) on ECG and some blood parameters in vitamin C treated and non-treated chickens. Veterinarski Arhiv, 76, 227- 235.
- Dönmez HH, Yörük M, Çınar A, Dönmez N (2007): Effects of vitamin C on ANAE positivity and blood cells in ACTH induced stress in chicken. Ind Vet J, 84, 1135- 1138.
- Dönmez N, Atalay B (2007): Sıcaklık stresi oluşturulan broylerlerde antibakteriyal etkili bitki ekstraktının (herbromix ®) bazı hematolojik parametreler üzerine etki- si. Ata Üniv Vet Bil Derg, 2, 82-86.
- Emre B, Sulu N, Hatipoğlu Ş, Çınar A (1994): C vitamini uygulanan ve uygulanmayan tavuklarda ACTH' nın glikoz ve insülin düzeylerine etkisi. Ankara Üniv Vet Fak Derg, 41, 10-17.
- Erdem E, Özdemir A, Kaya C, et al. (2012): Kronik böbrek yetmezliğinde serum ürik asit düzeyleri. Fırat Tıp Derg, 17, 23-27.
- Freeman BM (1975): Physiological basis of stres. Proc Roy Soc Med, 68, 427-429.
- Gölbaşı Z, Aydoğdu S, Sakallı M, et al. (1999): Kreatin kinaz-MB total kreatin kinazdan yüksek olabilir mi? Bir Olgu Sunusu. Türk Kardiol Dern Arş, 27, 515-517.
- Gray HG, Paradis TJ, Chang PW (1989): Research note: Physiological effects of adrenocorticotropic hormone and hydrocortisone in laying hens. Int J Poult Sci, 68, 1710-1713.
- Hall SJG, Broom DM, Goode JA, et al. (1999): Physiological responses of sheep during long road journeys involving ferry crossings. Anim Sci, 69, 19-27.
- Hill JA (1983): Indicators of stress in poultry. World’s Poult Sci J, 39, 24-32.
- Khan MR, Badar I, Siddiquah A (2011): Prevention of hepatorenal toxicity with Sonchusasper in gentamicin treated rats. BMC Complement Altern Med, 11,113.
- Matwichuk CL, Taylor SM, Shmon CL, et al. (1999): Changes in rectal temparature and hematologic, biochemical, blood gas, and acid-base values in healty Labrador Retrievers before and after strenuous exercise. Am J Vet Res, 1, 88-92.
- Mowbray BM, Bishop DM (1953): A.C.T.H. in diagnosis of adrenal insufficiency (Thorn Test). British Med J, 3, 17- 21.
- Nanda AS, Dobson H, Ward WR (1990): Relationship between an increase in plasma cortisol during transport- induced stress and failure of oestradiol to induce a luteinising hormone surge in dairy cows. Res Vet Sci, 49, 25-28.
- Öner AF, Arslan Ş, Özer R, et al. (1998): Steroid tedavi- si verilen hastalarda bazı biyokimyasal parametrelerin değerlendirilmesi. Van Tıp Derg, 5, 149-151.
- Pacak K, Palkovits M (2001): Stressor specificity of central neuroendocrine responses: implications for stress- related disorders. Endoc Rev, 22, 502-548.
- Polidori MC, Savino K, Alunni G, et al. (2002): Plasma lipophilic antioxidants and malondialdehyde in congestive heart failure patients: relationship to disease severity. Free Radic Biol Med, 15, 148-152.
- Puvadolpirod S, Thaxton JP (2000): Model of physiological stress in chickens 2. Dosimetry of adrenocorticotropi. Poult Sci, 79, 370-376.
- Reid IR, Grey AB (1993): Corticosteroid osteoporosis. Baillieres Clin Rheumatol 7, 573-587.
- Sapolsky RM, Romero LM, Munck, AU (2000): How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endoc Rev, 21, 55-89.
- Schneider PL, Beede DK, Wilcox CJ (1988): Nycterohemeral patterns of acid-base status, mineral concentrations and digestive function of lactating cows in natural or chamber heat stress environments. J Anim Sci, 66, 112-125.
- Siegel HS (1971): Adrenals, stress, and the environment. Worlds Poult Sci J, 27, 327-349.
- Simon J (1984): Effects of daily corticosterone injection upon plasma glicose, insulin, uric acid and electrolytes and food intake pattern in the chicken. Diabetes and Metabolism, 10, 211-217.
- Soliman KM, Abdul-Hamid M, Othman AI (2007): Effect of carnosine on gentamicin-induced nephrotoxicity. Med Sci Monit, 13, 73-83.
- Stevenson JR, Taylor R (1988): Effects of glucocrticoid and antiglucocorticoid hormones on leukocytes numbers and function. Int J Immunopharmacol, 10, 1-6.
- Topçu B (2009): Tavşanlarda ACTH ve Dekzametazon’un Oluşturduğu Kardiyovasküler Değişikliklere Karşı L- Karnitin’in Koruyucu Etkilerinin Araştırılması, Kafkas Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi, Kars.
- Ünal Ç (2010): Ratlarda Soğuk Stresinin Trioid Bezi Üze- rine Etkileri. Atatürk Üniversitesi, Sağlık Bilimleri Ensti- tüsü, Anatomi Anabilim Dalı, Erzurum.
- Zaidi SMKR, Al-Qirim TM, Banu N (2005): Effects of antioxidant vitamins on glutathione depletion and lipid peroxidation ınduced by restraint stress in the rat liver. Drugs R D, 6, 157-165. Geliş tarihi: 13.01.2015 / Kabul tarihi: 11.08.2015
Details
| Other ID | JA27KE36UH |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2016 |
| Published in Issue | Year 2016Volume: 63 Issue: 3 |