Köpek hepatoid bezi tümörlerinin tanısında ince iğne aspirasyon sitolojisi (İİAS): Histopatolojiyle karşılaştırmalı çalışma
Abstract
Bu çalışmada hepatoid bez tümörlerinin sitolojik ve histopatolojik bulguları karşılaştırılarak sitolojik tanının güvenirliği araştırıldı. Bu amaçla hepatoid bez tümörü şüphesi bulunan 32 farklı ırk, yaş ve cins köpekten alınan örnekler kullanıldı. Sitolojik örnekler İnce İğne Aspirasyon Sitolojisi (İİAS) tekniğine göre alındı, havada kurutularak May-Grünwald Giemsa (MGG) ile boyandı. Histopatolojik inceleme amacıyla alınan dokular ise %10’luk nötral formalin solüsyonunda tespit edilerek rutin doku takibine alınarak hematoksilen eozin (HE) ile boyandı. Çalışma sonucunda; 32 örneğin %84.37’sinde sitolojik ve histopatolojik tanının örtüştüğü, buna karşılık 5 olguda hatalı tanıya gidildiği görüldü. Sitolojik olarak yapılan incelemelerde 27 örneğin 18 (%66,7)’ ine adenom, 6 (%22,2)’ine karsinom ve 3 (%11,1)’üne benign-epitelyom tanısı konuldu. Histopatolojik olarak ise 32 olgunun 22 (%68,7)’ü hepatoid gland adenom (HGA), 7 (%21,9)’i hepatoid gland karsinom (HGK) ve 3 (%9,4) hepatoid gland epitelyom (HGE) olarak tespit edildi. Sonuç olarak köpeklerde hepatoid tümörlerde İİAS tekniği ve sitolojik tanının erken tanıda yararlı olabileceği kanısına varıldı
References
- Berrocal A, Vos JH, Van Den Ingh T, et al. (1989): Canine perianal tumors. Zentralbl Veterinarmed A, 36, 739-749.
- Berzina I, Sharkey LC, Matise I, et al. (2008): Correlation between cytologic and histopathologic diagnoses of bone lesions in dogs: a study of the diagnostic accuracy of bone cytology. Vet Clin Pathol, 37, 332-338.
- Betini G, Morini M, Campagna F, et al. (2005): True grit: the tale of a subcutaneous mass in a dog. Vet Clin Pathol, 34, 73-75.
- Casalli GD, Gobbi H, Malm C, et al. (2007): Evaluation of accuracy of fine needle aspiration cytology for diagnosis of canine mammary tumors: comparative features with human tumors. Cytopathology, 18, 191-196.
- Daskalopoulou D, Maounis N, Kokalis G, et al. (1993): The role of fine needle aspiration cytology in the diagnosis of primary skin tumors. Arch Anal Cytol Path, 41, 75-81.
- Genevois JP (1980): Pathologie ano-rectale et perineale, i circumanalomes. Revue Méd Vét, 131, 697-705.
- Ghisleni G, Roccabianca P, Ceruti R, et al. (2006): Correlation between fine-needle aspiration cytology and histopathology in the evaluation of cutaneous and subcutaneous masses from dogs and cats. Vet Clin Pathol, 35, 24-30.
- Goldschmidt MH, Hendrick MJ (2002): Tumors of the skin and soft tissues. In: Meuten DJ, ed. Tumors in domestic animals. 4th ed. Iowa State Press, Iowa, 45-117.
- Griffiths GL, Lumsden JH, Vali V (1984): Fine-needle aspiration cytology and histologic correlation in canine tumors. Vet Clin Pathol, 13, 13-17.
- Hall RL, Macwilliams PS (1988): The cytologic examination of cutaneous and subcutaneous masses. Semin Vet Med Surg, 3, 94.
- Hayes HM, Wilson G (1977): Hormone-dependent neoplasms of the canine perianal gland. Cancer Res, 37, 2068-2071.
- Haziroglu R, Yardimci B, Aslan S, et al. (2010): Cytological evaluation of canine mammary tumors with fine needle aspiration biopsy technique. Revue Méd Vét, 161, 212-218.
- Macneill AL (2011): Cytology of canine and feline cutaneous and subcutaneous lesions and lymph nodes. Top Companion Anim Med, 26, 62-76.
- Morrison WB, Denicola DB (1993): Advantages and disadvantages of cytology and histopathology for the diagnosis of cancer. Semin Vet Med Surg, 8, 222-227.
- Nielsen SW, Aftosmis J (1964): Canine perianal gland tumors. J Am Vet Med Assoc, 144, 127.
- Pisani G, Millanta F, Lorenzi D, et al. (2006): Androgen receptor expression in normal, hyperplastic and neoplastic perianal glands in the dog. Res Vet Sci, 81, 231-236.
- Sharif MAM (2006): Epidemiology of skin tumors entities according to the new WHO classification in dogs and cats. VVB, Laufersweiler Verlag, Giessen, Germany, 1-190.
- Simeonov R, Simeonova G (2008): Computer-Assisted nuclear morphometry in the cytological evaluation of canine perianal adenocarcinomas. J Comp Path, 139, 226- 230.
- Simeonov R, Stoikov D (2006): Study on the correlation between the cytological and histological tests in the diagnostics of canine spontaneous mammary neoplasms. BJVM, 9, 211-219.
- Vail DM, Withrow SJ, Schwarz PD, et al. (1990): Perianal adenocarcinomas in the canine male: a retrospective study of 41 cases. J Am Anim Hosp Assoc, 26, 329-334.
- Weiss E, Frese F (1974): Tumors of the skin. Bull Wld Hlth Org, 50, 79-100.
- Wellman ML (1990): The cytologic diagnosis of neoplasia. Vet Clin North Am Small Anim Prac, 20, 919- 937.
- Wilson GP, Hayes HM (1979): Castration for treatment of perianal gland neoplasms in the dog. J Am Vet Med Assoc, 174, 1301-1303.
- Yildirim F, Gurel A (2012): Comparison between cytological and histopathological evaluations of canine mammary tumors. Revue Méd Vét, 163, 116-122.
Fine needle aspiration cytology (FNAC) in the diagnosis of canine hepatoid gland tumors- A comparative study with histopathology
Abstract
In this study, cytological and histological findings of canine hepatoid gland tumors were evaluated for accuracy of cytologic diagnosis. Thirty two samples from dogs of different breed, sex and age were used in the study. Cytologic speciments were prepared by Fine Needle Aspiration Cytology (FNAC) and stained with May-Grünwald Giemsa (MGG). On the other hand, histopathologic tissue samples were obtained surgically. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with hematoxylin-eosin (H&E). In 84.37% of the samples taken from the 32 dogs included in the study, matching cytological and histopathological diagnoses were made, while 5 of the cases were misdiagnosed. Based on cytological examination, from 27 of the samples taken, 18 (66,7%) were diagnosed as Suspect adenoma, 6 (22,2%) as Suspect carcinoma and 3 (11,1%) as Suspect epithelioma. Based on histopathological examination, of the total 32 cases, 22 (68,7%) were diagnosed as Hepatoid gland adenoma (HGA), 7 (21,9%) as Hepatoid gland carcinoma (HGC) and 3 (9,4%) as Hepatoid gland epithelioma (HGE). These results suggest that the FNAC technique and cytological examination could be of use in the early diagnosis of canine hepatoid gland tumors
References
- Berrocal A, Vos JH, Van Den Ingh T, et al. (1989): Canine perianal tumors. Zentralbl Veterinarmed A, 36, 739-749.
- Berzina I, Sharkey LC, Matise I, et al. (2008): Correlation between cytologic and histopathologic diagnoses of bone lesions in dogs: a study of the diagnostic accuracy of bone cytology. Vet Clin Pathol, 37, 332-338.
- Betini G, Morini M, Campagna F, et al. (2005): True grit: the tale of a subcutaneous mass in a dog. Vet Clin Pathol, 34, 73-75.
- Casalli GD, Gobbi H, Malm C, et al. (2007): Evaluation of accuracy of fine needle aspiration cytology for diagnosis of canine mammary tumors: comparative features with human tumors. Cytopathology, 18, 191-196.
- Daskalopoulou D, Maounis N, Kokalis G, et al. (1993): The role of fine needle aspiration cytology in the diagnosis of primary skin tumors. Arch Anal Cytol Path, 41, 75-81.
- Genevois JP (1980): Pathologie ano-rectale et perineale, i circumanalomes. Revue Méd Vét, 131, 697-705.
- Ghisleni G, Roccabianca P, Ceruti R, et al. (2006): Correlation between fine-needle aspiration cytology and histopathology in the evaluation of cutaneous and subcutaneous masses from dogs and cats. Vet Clin Pathol, 35, 24-30.
- Goldschmidt MH, Hendrick MJ (2002): Tumors of the skin and soft tissues. In: Meuten DJ, ed. Tumors in domestic animals. 4th ed. Iowa State Press, Iowa, 45-117.
- Griffiths GL, Lumsden JH, Vali V (1984): Fine-needle aspiration cytology and histologic correlation in canine tumors. Vet Clin Pathol, 13, 13-17.
- Hall RL, Macwilliams PS (1988): The cytologic examination of cutaneous and subcutaneous masses. Semin Vet Med Surg, 3, 94.
- Hayes HM, Wilson G (1977): Hormone-dependent neoplasms of the canine perianal gland. Cancer Res, 37, 2068-2071.
- Haziroglu R, Yardimci B, Aslan S, et al. (2010): Cytological evaluation of canine mammary tumors with fine needle aspiration biopsy technique. Revue Méd Vét, 161, 212-218.
- Macneill AL (2011): Cytology of canine and feline cutaneous and subcutaneous lesions and lymph nodes. Top Companion Anim Med, 26, 62-76.
- Morrison WB, Denicola DB (1993): Advantages and disadvantages of cytology and histopathology for the diagnosis of cancer. Semin Vet Med Surg, 8, 222-227.
- Nielsen SW, Aftosmis J (1964): Canine perianal gland tumors. J Am Vet Med Assoc, 144, 127.
- Pisani G, Millanta F, Lorenzi D, et al. (2006): Androgen receptor expression in normal, hyperplastic and neoplastic perianal glands in the dog. Res Vet Sci, 81, 231-236.
- Sharif MAM (2006): Epidemiology of skin tumors entities according to the new WHO classification in dogs and cats. VVB, Laufersweiler Verlag, Giessen, Germany, 1-190.
- Simeonov R, Simeonova G (2008): Computer-Assisted nuclear morphometry in the cytological evaluation of canine perianal adenocarcinomas. J Comp Path, 139, 226- 230.
- Simeonov R, Stoikov D (2006): Study on the correlation between the cytological and histological tests in the diagnostics of canine spontaneous mammary neoplasms. BJVM, 9, 211-219.
- Vail DM, Withrow SJ, Schwarz PD, et al. (1990): Perianal adenocarcinomas in the canine male: a retrospective study of 41 cases. J Am Anim Hosp Assoc, 26, 329-334.
- Weiss E, Frese F (1974): Tumors of the skin. Bull Wld Hlth Org, 50, 79-100.
- Wellman ML (1990): The cytologic diagnosis of neoplasia. Vet Clin North Am Small Anim Prac, 20, 919- 937.
- Wilson GP, Hayes HM (1979): Castration for treatment of perianal gland neoplasms in the dog. J Am Vet Med Assoc, 174, 1301-1303.
- Yildirim F, Gurel A (2012): Comparison between cytological and histopathological evaluations of canine mammary tumors. Revue Méd Vét, 163, 116-122.
Details
| Other ID | JA59NN65ZK |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2016 |
| Published in Issue | Year 2016Volume: 63 Issue: 3 |