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Deneysel olarak Arcobacter butzleri ile infekte edilmiş Balb/c farelerde bakteriyel antijenin immunohistokimyasal dağılımı ve histopatolojik bulgular

Year 2016, Volume: 63 Issue: 3, 267 - 276, 01.09.2016

Ahmet Ata Bayram Latife Çakır

Abstract

Bu çalışmada BALB/c farelerde Arcobacter butzleri’nin (A.butzleri) patojenitesini ve doku kolonizasyonunu ortaya koymak amaçlanmıştır. Toplam 60 adet fare kullanıldı. Her birinin deney grubunda 16 adet, kontrollerinde ise 4’er adet fare kullanılan 3 grup oluşturuldu. Araştırma 15 günle sınırlandırıldı. A.butzleri izolatı ml'sinde 1x109 bakteri içerecek şekilde BALB/c farelere peroral (p.o), intraperitoneal (i.p) ve meme bezi içine (m.b.i) verilerek klinik ve patolojik bulgular değerlendirildi. BALB/c fareler üç inokulasyon yoluyla da infeksiyona duyarlı bulundular. Mortalite, i.p ve m.b.i inokulasyon gruplarında %95, p.o grupta ise %25 olarak saptandı. Klinik ve patolojik bulgular i.p. ve m.b.i. inokulasyon gruplarında gözlendi. İnfekte gruplarda, ishal, halsizlik, vücut ısısının düşmesi (i.p ve m.b.i); hepatomegali, splenomegali (i.p); infekte meme bezlerinde şişkinlik, eksudasyon, apse formasyonu (m.b.i) gözlendi. Histopatolojik olarak, akciğer, karaciğer, böbrek, dalak, bölgesel lenf düğümleri, mide, kolon ve nötrofil ve plazma hücreleri ile karakterize akut/kronik yangı, lokal nekroz ve konjesyon (i.p), meme bezi ve deri altı dokusunda ise şiddetli, diffuz, kronik pyogranulomatoz-nekrotik yangı ve intersitisyel fibrosis (m.b.i) saptandı. Tüm inokulasyon gruplarında Arcobacter butzleri inokülasyon sonrası 5. güne kadar izole edildi. Sonuç olarak, BALB/c farelerin Arcobacter butzleri’nin deneysel infeksiyonu için uygun deney hayvanı olarak kullanılabileceği kanısına varıldı

Keywords

Arcobacter butzleri BALB/c fare deneysel infeksiyon

References

  • Abay S, Kayman T, Hizlisoy H, et al. (2012): In vitro antibacterial susceptibility of Arcobacter butzleri isolated from different sources. J Vet Med Sci 74, 613-616.
  • Adesiji YO, Emikpe BO, Olatain JO (2009): Histopathological changes associated with experimental infection of Arcobacter butzleri in albino rats. SLJB, 1, 4- 9.
  • Adesiji YO, Emikpe BO, Opalekunde AB (2011): Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri. Acta Med Lituanica, 18, 127-131.
  • Adesiji YO, Seibu E, Emikpe BO, et al. (2012): Serum biochemistry and heamatological changes associated with graded doses of experimental arcobacter infection in rats. West Afr J Med, 31, 186-91.
  • Al-banna NA, Junaid TA, Mathew TC, et al. (2008): Histopathological and ultrastructural studies of a mouse lung model of Campylobacter jejuni infection. J Med Microbiol, 57, 210-217.
  • Anderson, KF, Kiehlbauch JA, Anderson DC, et al. (1993): Arcobacter (Campylobacter) butzleri associated diarrheal illness in a nonhuman primate population. Infect Immun, 61, 2220-2223.
  • Atabay HI, Aydin F, Houf K, et al. (2003): The prevalance of Arcobacter spp. on chicken carcases sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE. Int J Food Microiol, 81, 21-28.
  • Bucker R, Troeger H, Kleer J, et al. (2009): Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6 cells. J Infect Dis, 200, 756-764.
  • Chandler RL (1970): Experimental bacterial mastitis in the mouse. J Med Microbiol, 3, 273-282.
  • De Oliveira SJ, Baetz AL, Wesley IV, et al. (1997): Classification of Arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in Brazil. Vet. Microbiol, 57, 347-354.
  • De Oliveria SJ, Wesley IV, Baetz AL, et al. (1999): Arcobacter cryaerophilus and Arcobacter butzleri isolated from preputial fluid of boars and fattening pigs in Brazil. J. Vet Diagn Invest, 11, 462-464.
  • De Vries JJ, Arents NI, Manson WL (2008): Campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review. Eur J Appl Microbiol, 27, 1119-1123.
  • Diker KS, Haziroglu R, Diker FS (1992): Experimental infection Campylobacter coli. Res Vet Sci, 52, 123-125. mammary gland with
  • Ellis WA, Neill SD, O’brien JJ, et al. (1977): Isolation of Spirillum/Vibrio-like organisms from bovine fetuses. Vet Rec, 100, 451-452
  • Ellis WA, Neill SD, O’brien JJ, et al. (1978): Isolation of Spirillum-like organisms from pig fetuses. Vet Rec, 102, 106.
  • Ferreira S, Queiroz JA, Oleastro M, et al. (2015): Insights in the pathogenesis and resistance of Arcobacter: A review. Crit Rev Microbiol, 25, 1-20.
  • Kayman T, Abay S, Hizlisoy H, et al. (2012): Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters. J Med Microbiol, 61, 1439-1444.
  • Kiehlbauch JA, Brenner DJ, Nicholson MA, et al. (1991): Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol, 29, 376-385.
  • Lau SK, Woo PC, Teng JL, et al. (2002): Identification by 16S ribozomal RNA gene sequencing of Arcobacter butzleri in a patient with acute gangrenous appendicitis. Mol Path, 55, 182-185.
  • Lehner A, Tasara T, Stephan R (2005): Relevant aspects of Arcobacter spp. as potential foodborn pathogen. Int J Food Microbiol, 102, 127-135.
  • Logan EF, Neill SD, Mackie DP (1992): Mastitis in dairy cows associated with an aerotolerant Campylobacter. Vet Rec, 110, 229-230.
  • Mansfiled LP, Forsythe SJ (2000): Arcobacter butzleri, A. Skirrowii and A. cryaerophilus—potential emerging human pathogens. Rev Med Microbiol, 11, 161- 170.
  • On SLW, Jensen TK, Bille-Hansen V, et al. (2002): Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark. Vet Microbiol, 85, 159-167.
  • Vandamme P, Vancanneyt M, Pot B, et al. (1992): Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol, 42, 344-356.
  • Vandamme P, Pugina P, Benzi G, et al. (1992b): Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. J Clin Microbiol, 30, 2335-2337.
  • Vandenberg O, Dediste A, Houf K, et al. (2004): Arcobacter species in humans. Emerg Infect Dis, 10, 1863- 1867.
  • Wesley IV, Baetz AL, Larson DJ (1996): Infection of cesarean derived colostrum-deprived 1-day-old piglets with Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. Infec Immun, 64, 2295-2299.
  • Wesley IV, Baetz AL (1999): Natural and experimental infections of Arcobacter in poultry. Poultry Sci, 78, 536- 545.
  • Wybo I, Breynaert J, Lauwers S et al. (2004): Isolation of Arcobacter skirrowi from a patient with chronic diarrhea. J Clin Microbiol, 42, 1851-1852.
  • Yan JJ, Ko WC, Huang AH, et al. (2000): Arcobacter butzleri bacteremia in a patient with liver cirrhosis. J Formos Med Assoc, 99, 166-169. Geliş tarihi: 21.04.2015 / Kabul tarihi: 26.10.2015 Address for correspondence: Latife Çakır Bayram, DVM, PhD Associate Professor, University of Erciyes, Faculty of Veterinary Medicine, Department of Pathology, 38039, Kayseri, Turkey e-mail: lcakir@erciyes.edu.tr

Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with

Year 2016, Volume: 63 Issue: 3, 267 - 276, 01.09.2016

Ahmet Ata Bayram Latife Çakır

Abstract

The aim of this study was to determine the tissue colonization and pathogenicity of Arcobacter butzleri in BALB/c mice. For this purpose, 60 adult mice were distributed into three experimental groups consisting of 16 animals in each and into three control groups consisting of 4 animals in each. One ml of A. butzleri suspension containing 1x109 CFU/mL was inoculated by peroral (PO), intraperitoneal (IP) and intramammary (IMG) routes to BALB/c mice in experimental groups in order to determine the clinical and pathological findings. Animals in control groups received 1 mL of sterile saline solution. Duration of the experiment was limited for 15 days. The BALB/c mice were found to be susceptible to infection by the three ways of inoculation. In infected mice, diarrhea, weakness, decrease of body temperature (IP and IMG); hepatomegaly, splenomegaly (IP); swelling of mammary glands, exudation, abscess formation (IMG) were seen. Mortality was determined as 25% in PO inoculation group and as 95% in IP IMG inoculation groups. In histopathological examination, acute/chronic inflammation characterized with neutrophil and plasma cells and local necrosis or congestion (IP) were determined in the lungs, kidneys, regional lymph nodes, liver, spleen, stomach and colon. However, severe, diffuse and chronic pyogranulomatous and necrotizing inflammation and interstitial fibrosis were observed in mammary gland and its subcutaneous tissues (IMG). In all of inoculation groups, A. butzleri was reisolated until the 5th days of post inoculation. The results of this study have suggested that BALB/c mice might be an appropriate experimental animal model for studying A.butzleri infection

Keywords

Arcobacter butzleri BALB/c mouse experimental infection

References

  • Abay S, Kayman T, Hizlisoy H, et al. (2012): In vitro antibacterial susceptibility of Arcobacter butzleri isolated from different sources. J Vet Med Sci 74, 613-616.
  • Adesiji YO, Emikpe BO, Olatain JO (2009): Histopathological changes associated with experimental infection of Arcobacter butzleri in albino rats. SLJB, 1, 4- 9.
  • Adesiji YO, Emikpe BO, Opalekunde AB (2011): Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri. Acta Med Lituanica, 18, 127-131.
  • Adesiji YO, Seibu E, Emikpe BO, et al. (2012): Serum biochemistry and heamatological changes associated with graded doses of experimental arcobacter infection in rats. West Afr J Med, 31, 186-91.
  • Al-banna NA, Junaid TA, Mathew TC, et al. (2008): Histopathological and ultrastructural studies of a mouse lung model of Campylobacter jejuni infection. J Med Microbiol, 57, 210-217.
  • Anderson, KF, Kiehlbauch JA, Anderson DC, et al. (1993): Arcobacter (Campylobacter) butzleri associated diarrheal illness in a nonhuman primate population. Infect Immun, 61, 2220-2223.
  • Atabay HI, Aydin F, Houf K, et al. (2003): The prevalance of Arcobacter spp. on chicken carcases sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE. Int J Food Microiol, 81, 21-28.
  • Bucker R, Troeger H, Kleer J, et al. (2009): Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6 cells. J Infect Dis, 200, 756-764.
  • Chandler RL (1970): Experimental bacterial mastitis in the mouse. J Med Microbiol, 3, 273-282.
  • De Oliveira SJ, Baetz AL, Wesley IV, et al. (1997): Classification of Arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in Brazil. Vet. Microbiol, 57, 347-354.
  • De Oliveria SJ, Wesley IV, Baetz AL, et al. (1999): Arcobacter cryaerophilus and Arcobacter butzleri isolated from preputial fluid of boars and fattening pigs in Brazil. J. Vet Diagn Invest, 11, 462-464.
  • De Vries JJ, Arents NI, Manson WL (2008): Campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review. Eur J Appl Microbiol, 27, 1119-1123.
  • Diker KS, Haziroglu R, Diker FS (1992): Experimental infection Campylobacter coli. Res Vet Sci, 52, 123-125. mammary gland with
  • Ellis WA, Neill SD, O’brien JJ, et al. (1977): Isolation of Spirillum/Vibrio-like organisms from bovine fetuses. Vet Rec, 100, 451-452
  • Ellis WA, Neill SD, O’brien JJ, et al. (1978): Isolation of Spirillum-like organisms from pig fetuses. Vet Rec, 102, 106.
  • Ferreira S, Queiroz JA, Oleastro M, et al. (2015): Insights in the pathogenesis and resistance of Arcobacter: A review. Crit Rev Microbiol, 25, 1-20.
  • Kayman T, Abay S, Hizlisoy H, et al. (2012): Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters. J Med Microbiol, 61, 1439-1444.
  • Kiehlbauch JA, Brenner DJ, Nicholson MA, et al. (1991): Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol, 29, 376-385.
  • Lau SK, Woo PC, Teng JL, et al. (2002): Identification by 16S ribozomal RNA gene sequencing of Arcobacter butzleri in a patient with acute gangrenous appendicitis. Mol Path, 55, 182-185.
  • Lehner A, Tasara T, Stephan R (2005): Relevant aspects of Arcobacter spp. as potential foodborn pathogen. Int J Food Microbiol, 102, 127-135.
  • Logan EF, Neill SD, Mackie DP (1992): Mastitis in dairy cows associated with an aerotolerant Campylobacter. Vet Rec, 110, 229-230.
  • Mansfiled LP, Forsythe SJ (2000): Arcobacter butzleri, A. Skirrowii and A. cryaerophilus—potential emerging human pathogens. Rev Med Microbiol, 11, 161- 170.
  • On SLW, Jensen TK, Bille-Hansen V, et al. (2002): Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark. Vet Microbiol, 85, 159-167.
  • Vandamme P, Vancanneyt M, Pot B, et al. (1992): Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol, 42, 344-356.
  • Vandamme P, Pugina P, Benzi G, et al. (1992b): Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. J Clin Microbiol, 30, 2335-2337.
  • Vandenberg O, Dediste A, Houf K, et al. (2004): Arcobacter species in humans. Emerg Infect Dis, 10, 1863- 1867.
  • Wesley IV, Baetz AL, Larson DJ (1996): Infection of cesarean derived colostrum-deprived 1-day-old piglets with Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. Infec Immun, 64, 2295-2299.
  • Wesley IV, Baetz AL (1999): Natural and experimental infections of Arcobacter in poultry. Poultry Sci, 78, 536- 545.
  • Wybo I, Breynaert J, Lauwers S et al. (2004): Isolation of Arcobacter skirrowi from a patient with chronic diarrhea. J Clin Microbiol, 42, 1851-1852.
  • Yan JJ, Ko WC, Huang AH, et al. (2000): Arcobacter butzleri bacteremia in a patient with liver cirrhosis. J Formos Med Assoc, 99, 166-169. Geliş tarihi: 21.04.2015 / Kabul tarihi: 26.10.2015 Address for correspondence: Latife Çakır Bayram, DVM, PhD Associate Professor, University of Erciyes, Faculty of Veterinary Medicine, Department of Pathology, 38039, Kayseri, Turkey e-mail: lcakir@erciyes.edu.tr
There are 30 citations in total.

Details

Other ID JA63BM53SD
Journal Section Research Article
Authors

Ahmet Ata

Bayram Latife Çakır

Publication Date September 1, 2016
Published in Issue Year 2016Volume: 63 Issue: 3

Cite

APA Ata, A., & Çakır, B. L. (2016). Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 267-276.
AMA Ata A, Çakır BL. Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with. Ankara Univ Vet Fak Derg. September 2016;63(3):267-276.
Chicago Ata, Ahmet, and Bayram Latife Çakır. “Histopathological Findings and Immunohistochemical Distribution of Bacterial Antigen in Balb/C Mice Experimentally Infected With”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 3 (September 2016): 267-76.
EndNote Ata A, Çakır BL (September 1, 2016) Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 3 267–276.
IEEE A. Ata and B. L. Çakır, “Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with”, Ankara Univ Vet Fak Derg, vol. 63, no. 3, pp. 267–276, 2016.
ISNAD Ata, Ahmet - Çakır, Bayram Latife. “Histopathological Findings and Immunohistochemical Distribution of Bacterial Antigen in Balb/C Mice Experimentally Infected With”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/3 (September 2016), 267-276.
JAMA Ata A, Çakır BL. Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with. Ankara Univ Vet Fak Derg. 2016;63:267–276.
MLA Ata, Ahmet and Bayram Latife Çakır. “Histopathological Findings and Immunohistochemical Distribution of Bacterial Antigen in Balb/C Mice Experimentally Infected With”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 3, 2016, pp. 267-76.
Vancouver Ata A, Çakır BL. Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with. Ankara Univ Vet Fak Derg. 2016;63(3):267-76.