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Türkiye’de abortla ilişkili equid herpesvirus tip 1 izolatlarının gB geninin kısmi sekansı

Year 2016, Volume: 63 Issue: 3, 277 - 281, 01.09.2016

Veysel Soydal Ataseven Tuba Çiğdem Oğuzoğlu Ender Dinçer Dağalp Seval Bilge

Abstract

Equid herpesvirus 1 (EHV-1), kısraklarda sporadik veya epidemik tarzda genellikle gebeliğin son trimesterinde görülen abort olgularından sorumlu başlıca etkendir. Bu araştırmada, yeni dizayn edilen bir primer çifti kullanılarak, ülkemizde 2011 yılındaki doğum sezonundaki abort salgınından izole edilen Türkiye (TR) EHV-1 saha suşlarının glikoprotein B (gB) gen bölgesinin kısmi karakterizasyonu araştırılmıştır. Moleküler analiz sonuçları bu araştırmada izole edilen suşların, önceki yıllarda ülkemizde izole edilen EHV-1 suşlarına genetik olarak benzer; EHV-1 Avrupa suşlarıyla ise genetik yakınlığa sahip olduğunu göstermiştir. Ayrıca, bu araştırmada izole edilen suşlar ile GenBank veri tabanında kayıtlı diğer EHV-1 suşlarının aminoasit sekansları arasında farklılıklar tespit edilmiştir. Bu moleküler araştırma, ülkemizde EHV-1 enfeksiyonunun izlenmesi ve gelecekteki salgınlardan izole edilebilecek yeni suşların gB gen bölgesindeki değişikliklerin patogenezi ve hastalığın gelişimi üzerine etkilerinin belirlenmesine ışık tutacaktır

Keywords

Abort at at herpesvirus tip 1 (EHV-1) glikoprotein B geni Türkiye

References

  • Allen GP (2002): Epidemic disease caused by equine herpesvirus-1: recommendations for prevention and control. Eq Vet Educ, 14, 136-142.
  • Arii J, Wang J, Morimoto T, et al. (2010): A single amino-acid substitution in herpes simplex virus 1 envelope glycoprotein B at a site required for binding to the paired immunoglobulin-like type 2 receptor α (PILR α) abrogates PILR α-dependent viral entry and reduces pathogenesis. J Virol, 84, 10773-10783.
  • Ataseven VS, Bilge Dağalp S, Güzel M, et al. (2009): Prevalence herpesvirus-4 infections in equidae species in Turkey as determined by ELISA and multiplex nested PCR. Res Vet Sci, 86, 339-344. herpesvirus-1 and equine
  • Avcı O, Yavru S, Tokgöz S, et al. (2014): Detection of antibodies against equine herpesvirus-1 and equine herpesvirus-4 in horses in Southeast Anatolia by indirect ELISA. Acta Sci Vet, 42, 1250.
  • Barrandeguy M, Parreno V, Chambers T, et al. (1999): Viral infections of horses in Argentina. 520-522. In: U Wernery, JF Wade, JA Mumford, OR Kaaden (Eds.), Proceedings of the 8th International Conference Equine Infectious Diseases, Dubai, UAE. R&W Publications, Newmarket, UK.
  • Başkaya H, Keskintepe H, Doğruer M, et al. (1968): An outbreak of equine virus abortion in Turkey I. Isolation and identification of rhinopneumonitis virus in cell cultures. Vet J Ankara Univ, 15, 309-317.
  • Borchers K, Böttner D, Lieckfeldt D, et al. (2006): Characterization of equid herpesvirus 1 (EHV-1) related viruses from captive grevy’s zebra and blackbuck. J Vet Med Sci, 68, 757-760.
  • Borchers K, Lieckfeldt D, Ludwig A, et al. (2008): Detection of equid herpesvirus 9 DNA in trigeminal ganglia of a Burchell’s zebra from the Serengeti ecosystem. J Vet Med Sci, 70, 1377-1381.
  • Cowdhury S, Naderi M, Chouljenko VN, et al. (2012): Amino acid differences in glycoproteins B (gB), C (gC), H (gH) and L (gL) are associated with enhanced herpes simplex virus type-1 (McKrae) entry via the paired immunglobulin-like type-2 receptor α. Virol J, 9, 112.
  • Davison AJ, Eberle R, Ehlers B, et al. (2009): The order Herpesvirales. Arch Virol, 154, 171-177.
  • Eisenberg RJ, Atanasiu D, Cairns TM, et al. (2012): Herpes virus fusion and entry: A story with many characters. Viruses, 4, 800-832.
  • Fukushi H, Tomita T, Taniguchi A, et al. (1997): Gazelle herpesvirus 1: A new neurotropic herpesvirus immunologically related to equine herpesvirus 1. Virology, 226, 34-44.
  • Gerst S, Borchers K, Gower SM, et al. (2003): Detection of EHV-1 and EHV-4 in placental sections of naturally occuring EHV-1- and EHV-4-related abortions in the UK: use of the placental in diagnosis. Eq Vet J, 35, 430-433.
  • Ghanem YM, Fukushi H, Ibrahim ESM, et al. (2008): Molecular phylogeny of equine herpesvirus 1 isolates from onager, zebra and Thomson’s gazelle. Arch Virol, 153, 2297-2302.
  • Gilkerson JR, Love DN, Whalley JM (1998): Epidemiology of equine herpesvirus abortion: searching for clues to the future. Aust Vet J, 76, 675-676.
  • Gryspeerdt AC, Vandekerckhove AP, Baghi HB, et al. (2012): Expression of late viral proteins is restricted in nasal mucosal leucocytes but not in epithelial cells during early-stage equine herpes virus-1 infection. Vet J, 193, 576-578.
  • Gür S, Yapıcı O (2008): Equine Herpesvirus type 1 and 4 in Individually Reared Horses in Central and Western Turkey. Acta Vet Brno, 77, 609-613.
  • Hall TA (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/ 98/ NT. Nucleic Acids Symp. Series No. 41, 95-98.
  • Hussey GS (2012): Equine herpesvirus-1: What are we stil missing? Vet J, 193, 309-310.
  • Kasem S, Yamada S, Kiupel M, et al. (2008): Equine herpesvirus type 9 in giraffe with encephalitis. Emerg Infect Dis, 14, 1948-1949.
  • Kirisawa R, Endo A, Iwai H, et al. (1993): Detection and identification of equine herpesvirus-1 and -4 by polymerase chain reaction. Vet Microbiol, 36, 57-67.
  • Kydd JH, Davis-Poynter NJ, Birch J, et al. (2006): A molecular approach to the identification of cytotoxic T- lymphocyte epitopes within equine herpesvirus 1. J Gen Virol, 87, 2507-2515.
  • Liu C, Guo W, Xiang W et al. (2012): Complete genomic sequence of an equine herpesvirus type 8 Wh strain isolated from China. J Virol, 30, 5407.
  • Lunn DP, Davis-Poynter N, Flaminio MJBF, et al. (2009): Equine herpesvirus consensus statement. J Vet Int Med, 23, 450-461.
  • Matsumura T, Sugiura T, Imagawa H, et al. (1992): Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations. J Vet Med Sci, 54, 207-211.
  • Neubauer A, Braun B, Brandmüller C, et al. (1997): Analysis of the contributions of the equine herpesvirus 1 glycoprotein gB homolog to virus entry and direct cell to cell spread. Virology, 227, 281-294.
  • Singh BK, Yadav MP, Uppal PK, et al. (1999): National assessment of equine herpesvirus-1 infection among equidae in India. 578-579. In: U Wernery, JF Wade, JA Mumford, OR Kaaden (Eds.), Proceedings of the 8th International Conference Equine Infectious Diseases, Dubai, UAE. R&W Publications, Newmarket, UK.
  • Slater J (2007): Equine Herpesviruses. 134-153. In: D Sellon, M Long (Eds.), Equine Infectious Diseases, Chapter 13. Elsevier, Philadelphia, USA.
  • Smith KL, Allen GP, Branscum AJ, et al. (2010): The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. Vet Microbiol, 141, 5-11.
  • Szeredi L, Aupperle H, Steiger K (2003): Detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and in-situ hybridization techniques. J Comp Pathol, 129, 147-153.
  • Tamura K (1992): Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol Biol Evol, 9, 678-687.
  • Tamura K, Peterson D, Peterson N, et al. (2011): MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 28, 2731-2739.
  • Tekelioğlu BK, Matsumura T, Tsujimura K, et al. (2006): Detection of equine herpesvirus type 1 (EHV-1) DNA organs of neonatal dead foals in Turkey. J Eq Sci, 17, 23-26.
  • Turan N, Yıldırım F, Altan E, et al. (2012): Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey. Res Vet Sci, 93, 1504-1507.
  • Yılmaz S, Girgin H, Demiryonguç A, et al. (1995): EHV- 1 infection in purebred English mares in Türkiye Jokey Culub (TJK) İzmit farm, protective vaccinations and result obtained. Etlik Vet Mikrobiyol Derg, 8, 230-237.
  • Yılmaz H, Altan E, Turan N, et al. (2012): First report on neuropathogenic EHV-1 in Turkey. J Eq Vet Sci, 32, 525- 530. molecular detection of Geliş tarihi: 16.12.2014 / Kabul tarihi: 16.10.2015 Address for correspondence: Assoc. Prof. Dr. Veysel Soydal ATASEVEN MustafaKemal University, Faculty of Veterinary Medicine, Department of Virology, Hatay, Turkey; E-mail: soydalata@hotmail.com

Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey

Year 2016, Volume: 63 Issue: 3, 277 - 281, 01.09.2016

Veysel Soydal Ataseven Tuba Çiğdem Oğuzoğlu Ender Dinçer Dağalp Seval Bilge

Abstract

Equid herpesvirus 1 (EHV-1) is a major agent of large-scale outbreaks of abortion, and these abortions have been described as sporadic or epidemic cases in mares, generally during the last trimester of pregnancy. In this study, the partial characterization based on the glycoprotein B (gB) gene of Turkish (TR) EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in Turkey was investigated using a novel designed primer set for EHV-1. The molecular analysis of TR EHV-1 strains showed that genetically identical TR EHV-1 strains were still circulating in Turkey during different years and, these strains were closely related to the European EHV-1 strains. Furthermore, EHV-1 isolated in the present study and to EHV-1 strains published previously in the GenBank database were shown some differences for the aa sequences. This molecular report would be valuable for monitoring of EHV-1 infection in Turkey and determining the gB gene sequence of newly identified EHV-1 field strains from future outbreaks on the pathogenesis and severity of disease

Keywords

Abortion equid herpesvirus type 1 (EHV-1) glycoprotein B gene horse Turkey

References

  • Allen GP (2002): Epidemic disease caused by equine herpesvirus-1: recommendations for prevention and control. Eq Vet Educ, 14, 136-142.
  • Arii J, Wang J, Morimoto T, et al. (2010): A single amino-acid substitution in herpes simplex virus 1 envelope glycoprotein B at a site required for binding to the paired immunoglobulin-like type 2 receptor α (PILR α) abrogates PILR α-dependent viral entry and reduces pathogenesis. J Virol, 84, 10773-10783.
  • Ataseven VS, Bilge Dağalp S, Güzel M, et al. (2009): Prevalence herpesvirus-4 infections in equidae species in Turkey as determined by ELISA and multiplex nested PCR. Res Vet Sci, 86, 339-344. herpesvirus-1 and equine
  • Avcı O, Yavru S, Tokgöz S, et al. (2014): Detection of antibodies against equine herpesvirus-1 and equine herpesvirus-4 in horses in Southeast Anatolia by indirect ELISA. Acta Sci Vet, 42, 1250.
  • Barrandeguy M, Parreno V, Chambers T, et al. (1999): Viral infections of horses in Argentina. 520-522. In: U Wernery, JF Wade, JA Mumford, OR Kaaden (Eds.), Proceedings of the 8th International Conference Equine Infectious Diseases, Dubai, UAE. R&W Publications, Newmarket, UK.
  • Başkaya H, Keskintepe H, Doğruer M, et al. (1968): An outbreak of equine virus abortion in Turkey I. Isolation and identification of rhinopneumonitis virus in cell cultures. Vet J Ankara Univ, 15, 309-317.
  • Borchers K, Böttner D, Lieckfeldt D, et al. (2006): Characterization of equid herpesvirus 1 (EHV-1) related viruses from captive grevy’s zebra and blackbuck. J Vet Med Sci, 68, 757-760.
  • Borchers K, Lieckfeldt D, Ludwig A, et al. (2008): Detection of equid herpesvirus 9 DNA in trigeminal ganglia of a Burchell’s zebra from the Serengeti ecosystem. J Vet Med Sci, 70, 1377-1381.
  • Cowdhury S, Naderi M, Chouljenko VN, et al. (2012): Amino acid differences in glycoproteins B (gB), C (gC), H (gH) and L (gL) are associated with enhanced herpes simplex virus type-1 (McKrae) entry via the paired immunglobulin-like type-2 receptor α. Virol J, 9, 112.
  • Davison AJ, Eberle R, Ehlers B, et al. (2009): The order Herpesvirales. Arch Virol, 154, 171-177.
  • Eisenberg RJ, Atanasiu D, Cairns TM, et al. (2012): Herpes virus fusion and entry: A story with many characters. Viruses, 4, 800-832.
  • Fukushi H, Tomita T, Taniguchi A, et al. (1997): Gazelle herpesvirus 1: A new neurotropic herpesvirus immunologically related to equine herpesvirus 1. Virology, 226, 34-44.
  • Gerst S, Borchers K, Gower SM, et al. (2003): Detection of EHV-1 and EHV-4 in placental sections of naturally occuring EHV-1- and EHV-4-related abortions in the UK: use of the placental in diagnosis. Eq Vet J, 35, 430-433.
  • Ghanem YM, Fukushi H, Ibrahim ESM, et al. (2008): Molecular phylogeny of equine herpesvirus 1 isolates from onager, zebra and Thomson’s gazelle. Arch Virol, 153, 2297-2302.
  • Gilkerson JR, Love DN, Whalley JM (1998): Epidemiology of equine herpesvirus abortion: searching for clues to the future. Aust Vet J, 76, 675-676.
  • Gryspeerdt AC, Vandekerckhove AP, Baghi HB, et al. (2012): Expression of late viral proteins is restricted in nasal mucosal leucocytes but not in epithelial cells during early-stage equine herpes virus-1 infection. Vet J, 193, 576-578.
  • Gür S, Yapıcı O (2008): Equine Herpesvirus type 1 and 4 in Individually Reared Horses in Central and Western Turkey. Acta Vet Brno, 77, 609-613.
  • Hall TA (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/ 98/ NT. Nucleic Acids Symp. Series No. 41, 95-98.
  • Hussey GS (2012): Equine herpesvirus-1: What are we stil missing? Vet J, 193, 309-310.
  • Kasem S, Yamada S, Kiupel M, et al. (2008): Equine herpesvirus type 9 in giraffe with encephalitis. Emerg Infect Dis, 14, 1948-1949.
  • Kirisawa R, Endo A, Iwai H, et al. (1993): Detection and identification of equine herpesvirus-1 and -4 by polymerase chain reaction. Vet Microbiol, 36, 57-67.
  • Kydd JH, Davis-Poynter NJ, Birch J, et al. (2006): A molecular approach to the identification of cytotoxic T- lymphocyte epitopes within equine herpesvirus 1. J Gen Virol, 87, 2507-2515.
  • Liu C, Guo W, Xiang W et al. (2012): Complete genomic sequence of an equine herpesvirus type 8 Wh strain isolated from China. J Virol, 30, 5407.
  • Lunn DP, Davis-Poynter N, Flaminio MJBF, et al. (2009): Equine herpesvirus consensus statement. J Vet Int Med, 23, 450-461.
  • Matsumura T, Sugiura T, Imagawa H, et al. (1992): Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations. J Vet Med Sci, 54, 207-211.
  • Neubauer A, Braun B, Brandmüller C, et al. (1997): Analysis of the contributions of the equine herpesvirus 1 glycoprotein gB homolog to virus entry and direct cell to cell spread. Virology, 227, 281-294.
  • Singh BK, Yadav MP, Uppal PK, et al. (1999): National assessment of equine herpesvirus-1 infection among equidae in India. 578-579. In: U Wernery, JF Wade, JA Mumford, OR Kaaden (Eds.), Proceedings of the 8th International Conference Equine Infectious Diseases, Dubai, UAE. R&W Publications, Newmarket, UK.
  • Slater J (2007): Equine Herpesviruses. 134-153. In: D Sellon, M Long (Eds.), Equine Infectious Diseases, Chapter 13. Elsevier, Philadelphia, USA.
  • Smith KL, Allen GP, Branscum AJ, et al. (2010): The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. Vet Microbiol, 141, 5-11.
  • Szeredi L, Aupperle H, Steiger K (2003): Detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and in-situ hybridization techniques. J Comp Pathol, 129, 147-153.
  • Tamura K (1992): Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol Biol Evol, 9, 678-687.
  • Tamura K, Peterson D, Peterson N, et al. (2011): MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 28, 2731-2739.
  • Tekelioğlu BK, Matsumura T, Tsujimura K, et al. (2006): Detection of equine herpesvirus type 1 (EHV-1) DNA organs of neonatal dead foals in Turkey. J Eq Sci, 17, 23-26.
  • Turan N, Yıldırım F, Altan E, et al. (2012): Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey. Res Vet Sci, 93, 1504-1507.
  • Yılmaz S, Girgin H, Demiryonguç A, et al. (1995): EHV- 1 infection in purebred English mares in Türkiye Jokey Culub (TJK) İzmit farm, protective vaccinations and result obtained. Etlik Vet Mikrobiyol Derg, 8, 230-237.
  • Yılmaz H, Altan E, Turan N, et al. (2012): First report on neuropathogenic EHV-1 in Turkey. J Eq Vet Sci, 32, 525- 530. molecular detection of Geliş tarihi: 16.12.2014 / Kabul tarihi: 16.10.2015 Address for correspondence: Assoc. Prof. Dr. Veysel Soydal ATASEVEN MustafaKemal University, Faculty of Veterinary Medicine, Department of Virology, Hatay, Turkey; E-mail: soydalata@hotmail.com
There are 36 citations in total.

Details

Other ID JA64DM34JG
Journal Section Research Article
Authors

Veysel Soydal Ataseven

Tuba Çiğdem Oğuzoğlu

Ender Dinçer

Dağalp Seval Bilge

Publication Date September 1, 2016
Published in Issue Year 2016Volume: 63 Issue: 3

Cite

APA Ataseven, V. S., Oğuzoğlu, T. Ç., Dinçer, E., Bilge, D. S. (2016). Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 277-281.
AMA Ataseven VS, Oğuzoğlu TÇ, Dinçer E, Bilge DS. Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey. Ankara Univ Vet Fak Derg. September 2016;63(3):277-281.
Chicago Ataseven, Veysel Soydal, Tuba Çiğdem Oğuzoğlu, Ender Dinçer, and Dağalp Seval Bilge. “Partial Sequence of the GB Gene of Equid Herpesvirus Type 1 Isolates Associated With Abortion in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 3 (September 2016): 277-81.
EndNote Ataseven VS, Oğuzoğlu TÇ, Dinçer E, Bilge DS (September 1, 2016) Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 3 277–281.
IEEE V. S. Ataseven, T. Ç. Oğuzoğlu, E. Dinçer, and D. S. Bilge, “Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey”, Ankara Univ Vet Fak Derg, vol. 63, no. 3, pp. 277–281, 2016.
ISNAD Ataseven, Veysel Soydal et al. “Partial Sequence of the GB Gene of Equid Herpesvirus Type 1 Isolates Associated With Abortion in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/3 (September 2016), 277-281.
JAMA Ataseven VS, Oğuzoğlu TÇ, Dinçer E, Bilge DS. Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey. Ankara Univ Vet Fak Derg. 2016;63:277–281.
MLA Ataseven, Veysel Soydal et al. “Partial Sequence of the GB Gene of Equid Herpesvirus Type 1 Isolates Associated With Abortion in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 3, 2016, pp. 277-81.
Vancouver Ataseven VS, Oğuzoğlu TÇ, Dinçer E, Bilge DS. Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey. Ankara Univ Vet Fak Derg. 2016;63(3):277-81.