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Genç ve yaşlı köpeklerin yağ dokusundan elde edilen mezenkimal kök hücrelerin karakterizasyonu

Year 2016, Volume: 63 Issue: 3, 297 - 302, 01.09.2016

Sancak İrem Gül Asuman Özen Alev Gürol Bayraktaroğlu Ahmet Ceylan Pınar Can

Abstract

Mezenkimal kök hücrelerin veteriner hekimlik alanında kullanımı birçok hastalığın tedavisi için umut ışığı olarak görülmektedir. Yağ doku kökenli mezenkimal kök hücrelerin eldesinin kolay olması ve üretiminin ek bir maliyet getirmemesi sebebiyle yağ dokudan kök hücre üretimi en çok kullanılan yöntem olarak karşımıza çıkmaktadır. Yağ doku kökenli hücrelerin multilineage potansiyeline sahip olduğu bilinmektedir. Köpek yağ doku kaynaklı mezenkimal kök hücrelerin başarılı bir şekilde transplante edildiği de daha önceki çalışmalarda da bildirilmiştir. Bu çalışmada 3 adet yaşlı (5 yaş üzeri) ve 3 adet genç (6 ay ve altı) köpekten operasyon sırasında alınan yağ doku örneklerinden elde edilen mezenkimal kök hücrelerin karşılaştırılması, adipojenik, kondrojenik ve osteojenik farklılaşma potansiyeli, farklılaşma hızı, üreme hızı ve karakteristik özellikleri yönünden aralarındaki farkların ortaya konulması, lipoaspiratın içerdiği kök hücre potansiyelinin karşılaştırılması amaçlanmıştır

Keywords

Farklılaşma köpek mezenkimal kök hücre yağ doku

References

  • Can A (2014): Kök hücre. Akademisyen Tıp Kitabevi, Yenişehir, Ankara.
  • Csaki C, Matis U, Mobasheri A, et al. (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507- 20.
  • Diaz-Romero J, Quintin A, Schoenholzer E, et al. (2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. J Cell Physiol, 229, 1106-1117.
  • Gartner LP, Hiatt JL (1997): Colour textbook of histology. WB Sounders Company, Philadelphia, Pensylvania.
  • Gül Sancak İ, Özen A, Alparslan Pınarlı F, et al. (2014): Limbal Stem Cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Univ Vet Fak Derg, 20, 909-914.
  • Halvorsen YD, Bond A, Sen A, et al. (2001): Thiazolidinediones and glucocorticoids induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism, 50, 407-413. synergistically
  • Awad HA, Butler DL, Boivin GP, et al. (1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. Tissue Engineering, 5, 267-277.
  • Justesen J, Pedersen SB, Stenderup K, et al. (2004): Subcutaneous adipocytes can differentiate into bone- forming cells in vitro and in vivo. Tissue Eng, 10, 381-391.
  • Karaöz E, Aksoy A, Ayhan S, et al. (2009): Characterization of mesenchymal stem cells from rat bone marrow: potential and immunophenotypic markers. Histochem Cell Biol, 132, 533-546. properties, differentiation
  • Lettry V, Hosoya K, Takagi S, et al. (2010): Coculture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res, 58, 5-15.
  • Meliga E, Strem BM, Duckers HJ, et al. (2007): Adipose-derived cells. Cell Trans, 16, 963-970.
  • Miranville A, Heeschen C, Sengenes C, et al. (2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation, 110, 349- 355.
  • Mizuno H, Zuk PA, Zhu M, et al. (2002): Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg, 109, 199-209.
  • Orbay H, Tobita M, Mizuno H (2012): Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications. Stem Cells International Volume, Article ID 461718, 9. http://dx.doi.org/10.1155/2012/461718.
  • Ozen A, Gul Sancak I, Koch S, et al. (2013): Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). Mic Res, 1, 17-23.
  • Özen A, Gül Sancak İ (2014): Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı. Ankara Üniv Vet Fak Derg, 61, 79-84.
  • Özen A, Gül Sancak İ, Tiryaki M, et al. (2013): Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche, 2: 22-4. DOI: 10.5152/niche.2013.162
  • Planat-Benard V, Silvestre J S, Cousin B, et al. (2004): Plasticity of human adipose lineage cells toward endothelial perspectives. Circulation, 109, 656-663. and therapeutic
  • Rehman J, Traktuev D, Li J, et al. (2004): Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 109, 1292.
  • Safford KM, Hicok KC, Safford SD, et al. (2002): Neurogenic differentiation of murine and human adipose- derived stromal cells. Biochem Biophys Res Commun, 294, 371-379.
  • Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif. Feb, 44, 99-108.
  • Seo MJ, Suh SY, Bae YC, et al. (2005): Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun, 328, 258- 264.
  • Vieira NM, Brandalise V, Zucconi E, et al. (2010): Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells. Cell Transplantation, 19, 279-289.
  • Yamamoto N, Akamatsu H, Hasegawa S, et al. (2007): Isolation of multipotent stem cells from mouse adipose tissue. J Dermatol Sci, 48, 43-52.
  • Zuk PA, Zhu M, Mizuno H, et al. (2001): Multilineage cells from human adipose tissue: implications for cell- based therapies. Tissue Eng, 7, 211-28. Geliş tarihi: 24.04.2015 / Kabul tarihi: 27.11.2015 Address for correspondence: Prof. Dr. Asuman Özen Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 06110 Dışkapı, Ankara-Turkey. e-mail: ozen@veterinary.ankara.edu.tr

Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs

Year 2016, Volume: 63 Issue: 3, 297 - 302, 01.09.2016

Sancak İrem Gül Asuman Özen Alev Gürol Bayraktaroğlu Ahmet Ceylan Pınar Can

Abstract

The use of mesenchymal cells in veterinary medicine is considered to be promising for the treatment of several diseases. Due to adipose mesenchymal stem cells being readily available and the expansion of these cells not incurring any additional cost, the culturing of adipose mesenchymal cells has become a commonly applied method. Adipose mesenchymal cells are known to have a multilineage potential of differentiation. Previous research has demonstrated success in the transplantation of canine adipose mesenchymal cells. This study was aimed at the comparative evaluation of mesenchymal stem cells isolated from adipose tissue samples taken at surgery from 3 old dogs (above 5 years of age) and 3 young dogs (1 to 6-months-old or younger), and at the determination of any differences in the adipogenic, chondrogenic, and osteogenic differentiation potential, differentiation rate, proliferation rate, and characteristics of these cells. Furthermore, a comparison was made for the stem cell potential of the lipoaspirates

Keywords

Adipose tissue differentiation dog mesenchymal stem cell

References

  • Can A (2014): Kök hücre. Akademisyen Tıp Kitabevi, Yenişehir, Ankara.
  • Csaki C, Matis U, Mobasheri A, et al. (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507- 20.
  • Diaz-Romero J, Quintin A, Schoenholzer E, et al. (2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. J Cell Physiol, 229, 1106-1117.
  • Gartner LP, Hiatt JL (1997): Colour textbook of histology. WB Sounders Company, Philadelphia, Pensylvania.
  • Gül Sancak İ, Özen A, Alparslan Pınarlı F, et al. (2014): Limbal Stem Cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Univ Vet Fak Derg, 20, 909-914.
  • Halvorsen YD, Bond A, Sen A, et al. (2001): Thiazolidinediones and glucocorticoids induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism, 50, 407-413. synergistically
  • Awad HA, Butler DL, Boivin GP, et al. (1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. Tissue Engineering, 5, 267-277.
  • Justesen J, Pedersen SB, Stenderup K, et al. (2004): Subcutaneous adipocytes can differentiate into bone- forming cells in vitro and in vivo. Tissue Eng, 10, 381-391.
  • Karaöz E, Aksoy A, Ayhan S, et al. (2009): Characterization of mesenchymal stem cells from rat bone marrow: potential and immunophenotypic markers. Histochem Cell Biol, 132, 533-546. properties, differentiation
  • Lettry V, Hosoya K, Takagi S, et al. (2010): Coculture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res, 58, 5-15.
  • Meliga E, Strem BM, Duckers HJ, et al. (2007): Adipose-derived cells. Cell Trans, 16, 963-970.
  • Miranville A, Heeschen C, Sengenes C, et al. (2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation, 110, 349- 355.
  • Mizuno H, Zuk PA, Zhu M, et al. (2002): Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg, 109, 199-209.
  • Orbay H, Tobita M, Mizuno H (2012): Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications. Stem Cells International Volume, Article ID 461718, 9. http://dx.doi.org/10.1155/2012/461718.
  • Ozen A, Gul Sancak I, Koch S, et al. (2013): Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). Mic Res, 1, 17-23.
  • Özen A, Gül Sancak İ (2014): Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı. Ankara Üniv Vet Fak Derg, 61, 79-84.
  • Özen A, Gül Sancak İ, Tiryaki M, et al. (2013): Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche, 2: 22-4. DOI: 10.5152/niche.2013.162
  • Planat-Benard V, Silvestre J S, Cousin B, et al. (2004): Plasticity of human adipose lineage cells toward endothelial perspectives. Circulation, 109, 656-663. and therapeutic
  • Rehman J, Traktuev D, Li J, et al. (2004): Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 109, 1292.
  • Safford KM, Hicok KC, Safford SD, et al. (2002): Neurogenic differentiation of murine and human adipose- derived stromal cells. Biochem Biophys Res Commun, 294, 371-379.
  • Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif. Feb, 44, 99-108.
  • Seo MJ, Suh SY, Bae YC, et al. (2005): Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun, 328, 258- 264.
  • Vieira NM, Brandalise V, Zucconi E, et al. (2010): Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells. Cell Transplantation, 19, 279-289.
  • Yamamoto N, Akamatsu H, Hasegawa S, et al. (2007): Isolation of multipotent stem cells from mouse adipose tissue. J Dermatol Sci, 48, 43-52.
  • Zuk PA, Zhu M, Mizuno H, et al. (2001): Multilineage cells from human adipose tissue: implications for cell- based therapies. Tissue Eng, 7, 211-28. Geliş tarihi: 24.04.2015 / Kabul tarihi: 27.11.2015 Address for correspondence: Prof. Dr. Asuman Özen Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 06110 Dışkapı, Ankara-Turkey. e-mail: ozen@veterinary.ankara.edu.tr
There are 25 citations in total.

Details

Other ID JA32AR38VT
Journal Section Research Article
Authors

Sancak İrem Gül

Asuman Özen

Alev Gürol Bayraktaroğlu

Ahmet Ceylan

Pınar Can

Publication Date September 1, 2016
Published in Issue Year 2016Volume: 63 Issue: 3

Cite

APA Gül, S. İ., Özen, A., Bayraktaroğlu, A. G., Ceylan, A., et al. (2016). Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 297-302.
AMA Gül Sİ, Özen A, Bayraktaroğlu AG, Ceylan A, Can P. Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg. September 2016;63(3):297-302.
Chicago Gül, Sancak İrem, Asuman Özen, Alev Gürol Bayraktaroğlu, Ahmet Ceylan, and Pınar Can. “Characterization of Mesenchymal Stem Cells Isolated from the Adipose Tissue of Young and Old Dogs”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 3 (September 2016): 297-302.
EndNote Gül Sİ, Özen A, Bayraktaroğlu AG, Ceylan A, Can P (September 1, 2016) Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 3 297–302.
IEEE S. İ. Gül, A. Özen, A. G. Bayraktaroğlu, A. Ceylan, and P. Can, “Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs”, Ankara Univ Vet Fak Derg, vol. 63, no. 3, pp. 297–302, 2016.
ISNAD Gül, Sancak İrem et al. “Characterization of Mesenchymal Stem Cells Isolated from the Adipose Tissue of Young and Old Dogs”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/3 (September 2016), 297-302.
JAMA Gül Sİ, Özen A, Bayraktaroğlu AG, Ceylan A, Can P. Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg. 2016;63:297–302.
MLA Gül, Sancak İrem et al. “Characterization of Mesenchymal Stem Cells Isolated from the Adipose Tissue of Young and Old Dogs”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 3, 2016, pp. 297-02.
Vancouver Gül Sİ, Özen A, Bayraktaroğlu AG, Ceylan A, Can P. Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg. 2016;63(3):297-302.