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Koyun beta-laktoglobulin geni ekzon 2 bölgesine ait genetik polimorfizmin araştırılması ve bazı süt verim özellikleri ile ilişkisi

Year 2016, Volume: 63 Issue: 3, 323 - 328, 01.09.2016

Özge Özmen Selim Kul

Abstract

β-laktoglobulin (β-LGB) geni ruminantlarda en temel peynir altı suyu protenidir; gebelik ve laktasyon süresince meme bezi hücrelerinden salgılanmaktadır; biyolojik fonksiyonu ise henüz tam olarak bilinmemektedir. Dolayısıyla, bu çalışmanın amacı koyun β-LGB geni ekzon 2 bölgesine ait tek nükleotit polimorfizmlerinin (TNP) genotip frekanslarının saptanması ve bu olası TNP’lerin süt verim özellikleri ile ilişkisinin belirlenmesidir. Çalışmada üç koyun popülasyonunda belirlenen TNP’lerin genetik yapısı PCR-RFLP ve DNA dizi analizi metodu ile incelenmiştir. Araştırmada toplam 450 örnek olmak üzere Sakız, Akkaraman ve İvesi ırkı koyunlardan 150’şer adet kan ve süt örneği kullanılmıştır. İvesi koyun ırkı β-LGB geni ekzon 2 bölgesi için monomorfik bulunmuştur. Sakız koyun ırkında AB genotipi ile süt verimi arasında önemli bir ilişki bulunurken (P<0.001), AA genotipli bireylerin yüksek yağ yüzdesine (P<0.001) sahip olduğu gözlenmiştir. Ayrıca bu çalışmada, β-LGB geni ekzon 2 bölgesine ait g.121C>A SNP ilk kez tanımlanmıştır. Bu araştırmada, Sakız koyun ırkında β-LGB geninin süt verimi üzerine önemli bir etkisi olduğu belirlenmiştir, ancak elde edilen bu bulguların bu konuda yapılacak ilişkilendirme çalışmaları ile doğrulanması gerekmektedir

Keywords

β-laktoglobulin (β-LGB) koyun süt verim özellikleri

References

  • Akcapinar H (2000): Koyun Yetiştiriciliği, Ismat Press, Ankara, pp 20-25.
  • Amigo L, Recio I, Ramos M (2000): Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk—a review. Int Dairy J, 10,135-149.
  • Arora R, Bhatia S, Mishra BP, et al. (2010): Genetic polymorphism of the -lactoglobulin gene in native sheep from India. Biochem Genet, 48, 304-311.
  • Barillet F (2007): Genetic improvement for dairy production in sheep and goats. Small Rumin Res, 70, 60- 75.
  • Barillet F, Arranz JJ, Carta A (2005): Mapping quantitative trait loci for milk production and genetic polymorphisms of milk proteins in dairy sheep. Genet Sel Evol, 37,109-123.
  • Carta A, Casu S, Salaris S (2009): Invited review: Current state of genetic improvement in dairy sheep. J Dairy Sci, 92,5814-5833.
  • Celik S, Ozdemir S (2006): β-Lactoglobulin variants in awassi and Morkaraman Sheep and their association with the composition and rennet clotting time of the milk. Turk J Vet Anim Sci, 30, 539-544.
  • Chessa S, Bolla P, Dario C, et al. (2003): Polimorfismi genetici lattoproteici nella razza ovina gentile di puglia: Monitoraggio mediante focalizzazione isoelectrica. Sci Tecn Lat Cas, 54,1-8.
  • Corral JM, Padilla JA, Izquierdo M, et al. (2013): Detection and genetic characterization of ovine CSN1S2B polymorphisms and their associations with milk production traits. Livest Sci, 153,10-19.
  • Di Stasio L, Portolano B, Todaro M, et al. (1997): Effect of ovine -lactoglobulin phenotype on cheese yield and composition, in Proc. IDF Seminar Milk Protein Polymorphism. IDF Publishers, Brussels. pp. 324-327.
  • Elmaci C, Oner Y, Balcioglu SM (2006): Genetic polymorphism of β-lactoglobulin gene in native Turkish sheep breeds. Biochem Genet, 44, 7-8.
  • Erhardt G (1989): Genetic polymorphisms of - lactalbumin and Genet, 20,76-77.
  • Excoffier L, Laval G, Schneider S (2006): Arlequin Version 3.01. An Integrated Software Package for Population Genetics Data Analysis. Computational and Molecular Population Genetics Lab (CMPG), Inst. of Zoology Univ. of Berne. http://cmpg.unibe.ch/software/arlequin3/, March 2014 Accessed 17
  • FAO (Food and Agriculture Organization of The United Nations), Statistical Databases (http://faostat3.fao.org/home/E), Accessed 25 December 2014.
  • Feligini M, Parma P, Aleandri R, et al. (1998): PCR- RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim Genet, 29, 473-474.
  • Giaccone P, Di Stasio L, Macciotta NPP, et al. (2000): Effect of betalactoglobulin polymorphism on related traits of dairy ewes milk analysed by repeated measures design. J Dairy Res, 67,443-448.
  • Giambra IJ, Brandt H, Erhardt G (2014): Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep Small Rum Res, 121, 382-394.
  • Hall T (2007): BioEdit (http://www.mbio.ncsu.edu./BioEdit/bioedit.html). 12 Accessed 11 December 2014.
  • Ivancovic A, Dovc P (2004): Polymorphism of - lactoglobulin and sheep. Acta Agric Slov, 84,121-130.
  • Kawecka A, Radko A (2011): Genetic polymorphism of -lactoglobulin in sheep raised for milk production. J Appl Anim Res, 39, 68-71.
  • Leone P, Primiceri S, Fraghi A, et al. (1998): Polimorfismo dei loci s1 e -lactoglobulina in ovini di razza Sarda e Delle Langhe, in Proc. IV Congresso Nazionale Biodiversità Germoplasma Locale e Sua Valorizzazione, Alghero. pp. 8-11.
  • Moioli B, D’Andrea M, Pilla F (2007): Candidate genes affecting sheep and goat milk quality. Small Rumin Res, 68, 179-192.
  • Orford M, Hadjipavlou G, Tzamaloukas O, et al. (2012): A single nucleotide polymorphism in the acetyl- coenzyme A acyltransferase 2 (ACAA2) gene is associated with milk yield in Chios sheep. J Dairy Sci, 95, 3419-3427.
  • Orford M, Tzamaloukas O, Papachristoforou C, et al. (2010): Technical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds. J Dairy Sci, 93, 5996-5999.
  • Othman OE, El Fiky SA, Hassan NA, et al. (2012): Genetic polymorphism of whey protein genes -LG and - LA in three Egyptian sheep breeds. J Appl Biol Sci, 6, 25- 30.
  • Pietrola E, Carta A, Fraghi A, et al. (2000): Effect of - lactoglobulin locus on milk yield in Sarda ewes. Zoot Nutr Anim, 26, 129-133.
  • Ramos AM, Matos C, Bettencourt C, et al. (2002): Influence of aS1-casein, b-lactoglobulin and prolactin genotypes on milk production traits in two Portuguese sheep breeds, in 7th World Congress on Genetics Applied to Livestock Production, Montpellier. INRA, Paris.
  • Ramos AM, Matos CAP, Russo-Almeida PA, et al. (2009): Candidate genes for milk production traits in Portuguese dairy sheep. Small Rumin Res, 82, 117-121.
  • Rampilli M, Cecchi F, Giuliotti L, et al. (1997): The influence of protein distribution and coagulation properties in milk of Massese breed ewes, in Milk Protein Polymorphism. International Dairy Federation, Brussels, pp. 311-315.
  • Sambrook J, Fritsche F, Maniatis T (1989): Molecular Cloning: A Laboratory Manual. Cold Spring HarbourLab. Press, Cold Spring Harbour, New York.
  • Schlee P, Krause I, Rottmann O (1993): Genotyping of ovine -Lg alleles A and B using the PCR. Arch Tierz, 36, 519-523.
  • Schmoll F, Herget I, Hatzipanagiotou A, et al. (1999): Associations of beta-lactoglobulin variants with milk production, performance in milk sheep. Wiener Tieraerztliche Monatsschrift, 86, 57-60. and reproductive
  • Selvaggi M, Laudadio V, Dario C, et al. (2014): Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production. J Sci Food Agric, 94, 3090-3099.
  • Staiger EA, Thonney ML, Buchanan JW, et al. (2010): Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. J Dairy Sci, 93, 1736- 1742.
  • Stambekov SZ, Shapiro YO, Mandrusova EE (1997): Milk protein polymorphism in Soviet Merino and Latvian Dark headed ewes and its connection with economically valuable characteristics. J Dairy Sci, 39, 6873.
  • Unal N, Akcapinar H, Atasoy F, et al. (2008): Milk yield and milking traits measured with different methods in Bafra sheep. Rev Med Vet, 159, 494-501.
  • Wu XL, MacNeil MD, De S, et al. (2005): Evaluation of candidate gene effects for beef backfat via Bayesian model. Genetica, 125, 103-113.
  • Yousefi S, Ahani Azari M, Zerehdaran S, et al. (2013): Effect of -lactoglobulin and -casein genes polymorphism on milk composition in indigenous Zel sheep. Arch Tierz, 56, 216-224. Geliş tarihi: 10.06.2015 / Kabul tarihi: 13.07.2015 Address for correspondence: Dr. Özge Özmen Ankara University, Faculty of Veterinary Medicine, Department of Genetics, Ankara, Turkey. e-mail: ozgeozmen@ankara.edu.tr

Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits

Year 2016, Volume: 63 Issue: 3, 323 - 328, 01.09.2016

Özge Özmen Selim Kul

Abstract

β-lactoglobulin (β-LGB) gene is the major whey protein of ruminant and is synthesized by secreting cells of mammary gland during pregnancy and lactation and its biological function is still unclear. Therefore, the purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine β-LGB gene exon 2 region and its possible association with milk traits in dairy sheep breeds. The genetic structures of the SNPs were examined by PCR-RFLP and DNA sequencing in three sheep populations. A hundred fifty blood and milk samples each from Sakiz, Akkaraman and Awassi ewes, with total of 450 samples were used in the experiment. Awassi sheep breed was found as monomorphic for β-LGB gene in exon 2 region. Genotype AB showed a strong association with milk yields content (P<0.001), whereas the animals carrying AA genotype had a higher fat percentage (P<0.001) value in the Sakiz sheep breeds. Furthermore, we have reported for the first time g.121C>A SNPs of the β-LGB gene for exon 2. In this research, β-LGB showed a significant effect on milk yield in the Sakiz ewes, but further studies are necessary to confirm the association reported here

Keywords

β-lactoglobulin (β-LGB) milk production traits sheep

References

  • Akcapinar H (2000): Koyun Yetiştiriciliği, Ismat Press, Ankara, pp 20-25.
  • Amigo L, Recio I, Ramos M (2000): Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk—a review. Int Dairy J, 10,135-149.
  • Arora R, Bhatia S, Mishra BP, et al. (2010): Genetic polymorphism of the -lactoglobulin gene in native sheep from India. Biochem Genet, 48, 304-311.
  • Barillet F (2007): Genetic improvement for dairy production in sheep and goats. Small Rumin Res, 70, 60- 75.
  • Barillet F, Arranz JJ, Carta A (2005): Mapping quantitative trait loci for milk production and genetic polymorphisms of milk proteins in dairy sheep. Genet Sel Evol, 37,109-123.
  • Carta A, Casu S, Salaris S (2009): Invited review: Current state of genetic improvement in dairy sheep. J Dairy Sci, 92,5814-5833.
  • Celik S, Ozdemir S (2006): β-Lactoglobulin variants in awassi and Morkaraman Sheep and their association with the composition and rennet clotting time of the milk. Turk J Vet Anim Sci, 30, 539-544.
  • Chessa S, Bolla P, Dario C, et al. (2003): Polimorfismi genetici lattoproteici nella razza ovina gentile di puglia: Monitoraggio mediante focalizzazione isoelectrica. Sci Tecn Lat Cas, 54,1-8.
  • Corral JM, Padilla JA, Izquierdo M, et al. (2013): Detection and genetic characterization of ovine CSN1S2B polymorphisms and their associations with milk production traits. Livest Sci, 153,10-19.
  • Di Stasio L, Portolano B, Todaro M, et al. (1997): Effect of ovine -lactoglobulin phenotype on cheese yield and composition, in Proc. IDF Seminar Milk Protein Polymorphism. IDF Publishers, Brussels. pp. 324-327.
  • Elmaci C, Oner Y, Balcioglu SM (2006): Genetic polymorphism of β-lactoglobulin gene in native Turkish sheep breeds. Biochem Genet, 44, 7-8.
  • Erhardt G (1989): Genetic polymorphisms of - lactalbumin and Genet, 20,76-77.
  • Excoffier L, Laval G, Schneider S (2006): Arlequin Version 3.01. An Integrated Software Package for Population Genetics Data Analysis. Computational and Molecular Population Genetics Lab (CMPG), Inst. of Zoology Univ. of Berne. http://cmpg.unibe.ch/software/arlequin3/, March 2014 Accessed 17
  • FAO (Food and Agriculture Organization of The United Nations), Statistical Databases (http://faostat3.fao.org/home/E), Accessed 25 December 2014.
  • Feligini M, Parma P, Aleandri R, et al. (1998): PCR- RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim Genet, 29, 473-474.
  • Giaccone P, Di Stasio L, Macciotta NPP, et al. (2000): Effect of betalactoglobulin polymorphism on related traits of dairy ewes milk analysed by repeated measures design. J Dairy Res, 67,443-448.
  • Giambra IJ, Brandt H, Erhardt G (2014): Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep Small Rum Res, 121, 382-394.
  • Hall T (2007): BioEdit (http://www.mbio.ncsu.edu./BioEdit/bioedit.html). 12 Accessed 11 December 2014.
  • Ivancovic A, Dovc P (2004): Polymorphism of - lactoglobulin and sheep. Acta Agric Slov, 84,121-130.
  • Kawecka A, Radko A (2011): Genetic polymorphism of -lactoglobulin in sheep raised for milk production. J Appl Anim Res, 39, 68-71.
  • Leone P, Primiceri S, Fraghi A, et al. (1998): Polimorfismo dei loci s1 e -lactoglobulina in ovini di razza Sarda e Delle Langhe, in Proc. IV Congresso Nazionale Biodiversità Germoplasma Locale e Sua Valorizzazione, Alghero. pp. 8-11.
  • Moioli B, D’Andrea M, Pilla F (2007): Candidate genes affecting sheep and goat milk quality. Small Rumin Res, 68, 179-192.
  • Orford M, Hadjipavlou G, Tzamaloukas O, et al. (2012): A single nucleotide polymorphism in the acetyl- coenzyme A acyltransferase 2 (ACAA2) gene is associated with milk yield in Chios sheep. J Dairy Sci, 95, 3419-3427.
  • Orford M, Tzamaloukas O, Papachristoforou C, et al. (2010): Technical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds. J Dairy Sci, 93, 5996-5999.
  • Othman OE, El Fiky SA, Hassan NA, et al. (2012): Genetic polymorphism of whey protein genes -LG and - LA in three Egyptian sheep breeds. J Appl Biol Sci, 6, 25- 30.
  • Pietrola E, Carta A, Fraghi A, et al. (2000): Effect of - lactoglobulin locus on milk yield in Sarda ewes. Zoot Nutr Anim, 26, 129-133.
  • Ramos AM, Matos C, Bettencourt C, et al. (2002): Influence of aS1-casein, b-lactoglobulin and prolactin genotypes on milk production traits in two Portuguese sheep breeds, in 7th World Congress on Genetics Applied to Livestock Production, Montpellier. INRA, Paris.
  • Ramos AM, Matos CAP, Russo-Almeida PA, et al. (2009): Candidate genes for milk production traits in Portuguese dairy sheep. Small Rumin Res, 82, 117-121.
  • Rampilli M, Cecchi F, Giuliotti L, et al. (1997): The influence of protein distribution and coagulation properties in milk of Massese breed ewes, in Milk Protein Polymorphism. International Dairy Federation, Brussels, pp. 311-315.
  • Sambrook J, Fritsche F, Maniatis T (1989): Molecular Cloning: A Laboratory Manual. Cold Spring HarbourLab. Press, Cold Spring Harbour, New York.
  • Schlee P, Krause I, Rottmann O (1993): Genotyping of ovine -Lg alleles A and B using the PCR. Arch Tierz, 36, 519-523.
  • Schmoll F, Herget I, Hatzipanagiotou A, et al. (1999): Associations of beta-lactoglobulin variants with milk production, performance in milk sheep. Wiener Tieraerztliche Monatsschrift, 86, 57-60. and reproductive
  • Selvaggi M, Laudadio V, Dario C, et al. (2014): Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production. J Sci Food Agric, 94, 3090-3099.
  • Staiger EA, Thonney ML, Buchanan JW, et al. (2010): Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. J Dairy Sci, 93, 1736- 1742.
  • Stambekov SZ, Shapiro YO, Mandrusova EE (1997): Milk protein polymorphism in Soviet Merino and Latvian Dark headed ewes and its connection with economically valuable characteristics. J Dairy Sci, 39, 6873.
  • Unal N, Akcapinar H, Atasoy F, et al. (2008): Milk yield and milking traits measured with different methods in Bafra sheep. Rev Med Vet, 159, 494-501.
  • Wu XL, MacNeil MD, De S, et al. (2005): Evaluation of candidate gene effects for beef backfat via Bayesian model. Genetica, 125, 103-113.
  • Yousefi S, Ahani Azari M, Zerehdaran S, et al. (2013): Effect of -lactoglobulin and -casein genes polymorphism on milk composition in indigenous Zel sheep. Arch Tierz, 56, 216-224. Geliş tarihi: 10.06.2015 / Kabul tarihi: 13.07.2015 Address for correspondence: Dr. Özge Özmen Ankara University, Faculty of Veterinary Medicine, Department of Genetics, Ankara, Turkey. e-mail: ozgeozmen@ankara.edu.tr
There are 38 citations in total.

Details

Other ID JA68TU67PP
Journal Section Research Article
Authors

Özge Özmen

Selim Kul

Publication Date September 1, 2016
Published in Issue Year 2016Volume: 63 Issue: 3

Cite

APA Özmen, Ö., & Kul, S. (2016). Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 323-328.
AMA Özmen Ö, Kul S. Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Univ Vet Fak Derg. September 2016;63(3):323-328.
Chicago Özmen, Özge, and Selim Kul. “Investigating the Genetic Polymorphism in the Exon 2 Region of Ovine Beta-Lactoglobulin Gene and Its Association With Some Milk Traits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no. 3 (September 2016): 323-28.
EndNote Özmen Ö, Kul S (September 1, 2016) Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 3 323–328.
IEEE Ö. Özmen and S. Kul, “Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits”, Ankara Univ Vet Fak Derg, vol. 63, no. 3, pp. 323–328, 2016.
ISNAD Özmen, Özge - Kul, Selim. “Investigating the Genetic Polymorphism in the Exon 2 Region of Ovine Beta-Lactoglobulin Gene and Its Association With Some Milk Traits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/3 (September 2016), 323-328.
JAMA Özmen Ö, Kul S. Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Univ Vet Fak Derg. 2016;63:323–328.
MLA Özmen, Özge and Selim Kul. “Investigating the Genetic Polymorphism in the Exon 2 Region of Ovine Beta-Lactoglobulin Gene and Its Association With Some Milk Traits”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 63, no. 3, 2016, pp. 323-8.
Vancouver Özmen Ö, Kul S. Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Univ Vet Fak Derg. 2016;63(3):323-8.