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A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats

Year 2015, Volume: 62 Issue: 2, 87 - 91, 01.06.2015

Sema Uslu Mecit Yörük

https://doi.org/10.1501/Vetfak_0000002663
Cited By: 2

Abstract

This study was performed to specify the distribution and the heterogeneity of mast cells found in lungs and trachea of Van Cats. Four Van Cats were used in the study. Appropriate sized pieces were taken from the lungs and tracheas of the cats. After BLA (Basic Lead Acetate-Mota) was set, it was blocked with paraplast by doing the routine tissue follow-up. Sections taken in 6-7 μm thickness were stained with 0,5 % toluidine blue and alcian-blue safranin O combined stain. Numbers of mast cells were specified semi-quantitatively in the sections stained with toluidine blue under lens 40. Among analysed organs, the most mast cells were found in lungs. To specify the granule construction, BLA detection was determined to be suitable. In the stain of Alcianblue safranin O, SO (+), AB (+) granulose mast cells were found; mast cells that have mixed granules were not found

Keywords

Heterogeneity light microscope lung mast cell trachea Van Cats

References

  • Bachelet CM, Bernaudin JF, Feith JF (1988): Distribution and pulmonary mast cells in the rat and guinea pig. Int Arc Allergy Appl Immunol, 87, 225-229.
  • Bancroft JD, Cook HC (1984): Manual of Histological Techniques. Churchill Livingstone, London.
  • Banks WJ (1986): Applied Veterinary Histology, pp: 86- 87, Second Edition, Williams and Wilkins Co Baltimore.
  • Barett KE, Metcalfe DD (1987): Heterogeneity of mast cells in the tissues of the respiratory tract and other organ systems. Am Rev Resp Dis, 135, 1190-1195.
  • Becker AB, Chung KF (1985): Mast cells heterogeneity in dog skin. Anat Record, 213, 477-480.
  • Chen W, Alley MR, Manktelow BW, Davey P (1990a): Mast cells in the ovine lower respiratory tract: Heterogeneity, morphology and density. Int Archs Allergy Appl Immunol, 93, 99-106.
  • Chen W, Alley MR, Manktelow BW, Slack P (1990b): Mast cells in the bovine lower respiratory tract: morphology, density and distribution. Brit Vet J, 146, 425- 436.
  • Enerback L (1966b): Mast cells in the rat gastrointestinal mucosa: I. Effects of fixation. Acta Path Microb Scandinav, 66, 298-302.
  • Enerback L (1966a): Mast cells in the rat gastrointestinal mucosa II. Dye-binding and metachromatic properties. Acta Path Microb Scandinav, 66, 303-312.
  • Eren Ü, Aştı RN, Kurtdede N, Sandıkçı M, Sur E (1999): İnek uterusunda mast hücrelerinin histolojik ve histokimyasal özellikleri ve mast hücre heterojenitesi. Tr J Vet Anim Sci, 23, 193-201.
  • Fox B, Bull TB, Guz A (1981): Mast cells in the human alveolar wall: an electron microscopic study. J Clin Pathol, 34, 1333-1342.
  • Fritz FJ, Pabst R (1989): Numbers and heterogeneity of mast cells in the genital tract of the rat. Int Arch Allergy Appl Immunol, 88, 360-362.
  • Galli SJ (1990): New insights in to “The ridle of mast cells” micro environmental regulation of mast cell development and phenotypic heterogeneity. Lab Invest, 62, 5-33.
  • Guerzon GM, Pare PD, Michoud MC, Hogg JC (1979): The number and distribution of mast cells in monkey lungs. Am Rev Respir Dis, 119, 59-66.
  • Güre A (1993): Van Kedisi. In, Abdulkadiroğlu A, Yiğit M, Oğuzbaşaran B (Eds): Van Kütüğü. No: 8, Yüzüncü Yıl Üniversitesi, Van, pp. 722-726.
  • Junquiera LC, Carneiro J (2003): Temel Histoloji (Basic Histology). Çeviri Editörü: Y. Aytekin, S. Solakoğlu. pp: 101-102, Nobel Tıp Kitapevleri.
  • Karaca T (2003): Tavuk ve bıldırcınlarda sindirim sisteminde bulunan mast hücrelerinin dağılımı ve heterojenitesi araştırmalar. Doktora Tezi. YYÜ Sağlık Bilimleri Enstitüsü, Van. morfolojik ve histometrik
  • Karaca T, Yörük M, Şimşek N (2006): Age related distribution of mast cells in the trachea and lung of chicken. Indian Vet J, 83, 649-651.
  • Katz HR, Stewens RL, Austen KF (1985): Heterogeneity of mammalian mast cells differentiated in vivo and in vitro. J Allergy Clin Immunol, 76, 250-259.
  • Kitamura Y, Kanakura Y, Sonoda S, Asai H, Nakano T (1987): Mutual phenotypic changes between connective tissue type and mucosal mast cells. Int Arc Allergy Applied Immun, 82, 244-248.
  • Koçak-Harem M (2001): Tavukların alt solunum yollarındaki mast hücreleri üzerine histolojik araştırmalar. Doktora Tezi. AÜ Sağlık Bilimleri Enstitüsü, Ankara.
  • Kurtdede N, Aştı RN, Ergün L, Ergün E (2000): Ankara keçilerinin alt solunum yolları mast hücreleri üzerine histolojik çalışmalar. AÜ Vet Fak Derg, 47, 339-349.
  • Küther K, Audige L, Kube P, Welle M (1998): Bovine mast cells: distribution, density, heterogeneity and influence of fixation techniques. Cell Tissue Res, 293, 111-119.
  • Mair TS, Stokes CR, Bourne FJ (1988): Distribution and ultrastructure of mast cells in the equine respiratory tract. Equi Vet J, 20, 54-58.
  • Majeed SK (1994): Mast cell distribution in rats. Drug Res, 44, 3.
  • Overveld FJV, Houben LAMJ, Schmitz FEM, Bruijnzeel PLB, Raaijmakers JAM, Terpstra GK (1989): Mast cells heterogeneity in human lung tissue. Clin Sci, 77, 297-304.
  • Özen A, Bayraktaroğlu AG, Ertuğrul T, Özcan Z, Ceylan A, Özen D (2014): Domuz oviduktunda mast hücreleri üzerinde ışık ve electron mikroskobik çalışmalar. Ankara Üniv Vet Fak Derg, 61, 9-14.
  • Özen A, Ergün L, Ergün E, Şimşek N (2007): Morphological studies on ovarian mast cells in the cow. Turk J Vet Anim Sci, 31, 131-136.
  • Pesci A, Rossi GA, Bertorelli G, Aufiero A, Zanon P, Olivieri D (1994): Mast cells in the airway lumen and bronchial mucosa of patients with chronic bronchitis. Am J Resp Crit Care Med, 149, 1311-1316.
  • Schwartz LB, Austen KF (1984): Structure and function of the chemical mediators of mast cells. Prog Allergy (Karger Basel), 34, 271-321.
  • Shanahan F, Niven IM, Dyck N, Denburg JA, Binenstock J, Befus AD (1987): Human lung mast cells: distribution and abundance of histochemically distinct subpopulations. Int Archs Allergy Appl Immun, 83, 329- 331.
  • Tung W (1991): Mast cells in the chick digestive tract II. fixation, distribution, histochemistry and ultrastructure. Tokai J Exp Clin Med, 16, 27-32.
  • Uslu S, Yörük M (2013): Yerli ördek (Anas Platyrhynchase) ve kaz’ın (Anser anser) alt solunum yolları ve akciğerlerinde bulunan mast hücrelerinin dağılımı ve heterojenitesi üzerine morfolojik ve histometrik araştırmalar. Kafkas Univ Vet Fak Derg, 19, 475-482.
  • Warton JM, Papadimitriou RG, Goldie RG, Paterson JW (1986): An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung. Aust J Exp Biol Med Sci, 64, 435-44.
  • Wilkes LK, McMenamin C, Holt PG (1991): Postnatal maturation of mast cell subpopulations in the rat respiratory tract. Immunology, 75, 535-541.
  • Xaubet A, Moises JA, Agusti C, Martos JA, Picado C (1991): Identification of mast cells in bronchoalveolar lavage fluid. Allergy, 46, 222-227. Geliş tarihi: 30.04.2014/ Kabul tarihi: 02.07.2014 Address for correspondence: Dr. Sema Uslu Yüzüncü Yıl University, Faculty of Veterinary Sciences, Departments of Histology and Embryology, 65080 Kampus / VAN - TURKEY e-mail: semauslu43@hotmail.com

Van Kedilerinin trakeya ve akciğerlerinde bulunan mast hücrelerinin dağılımı ve heterojenitesiüzerine morfolojik ve histometrik çalışma

Year 2015, Volume: 62 Issue: 2, 87 - 91, 01.06.2015

Sema Uslu Mecit Yörük

https://doi.org/10.1501/Vetfak_0000002663
Cited By: 2

Abstract

Bu çalışma Van Kedilerinde trakeya ve akciğerlerde bulunan mast hücrelerinin dağılımı ve heterojenitesini belirlemek amacıyla yapıldı. Çalışmada 4 adet Van Kedisi kullanıldı. Van Kedilerinin trakeya ve akciğerlerinden uygun büyüklükte parçalar alındı. BLA (Basic Kurşun Asetat-Mota)’da tespit edildikten sonra rutin doku takibi yapılarak paraplast ile bloklandı. 6-7 µm kalınlığında alınan kesitler % 0,5’lik toluidine blue ve alcian blue-safranin O kombine boyalarında boyandılar. Toluidine blue ile boyanan kesitlerde 40’lık objektifte mast hücre sayıları semikantitatif olarak belirlendi. İncelenen organlardan akciğerde en fazla sayıda mast hücresine rastlandı. Granül yapısını belirlemede BLA tespitinin uygun olduğu saptandı. Alcian blue- safranin O boyamasında ise trakeya ve akciğerde SO (+), AB (+) granüllü mast hücrelerine rastlandı, miks granüle sahip mast hücrelerine ise rastlanılamadı

Keywords

Akciğer heterojenite ışık mikroskobu mast hücresi trakeya Van Kedisi

References

  • Bachelet CM, Bernaudin JF, Feith JF (1988): Distribution and pulmonary mast cells in the rat and guinea pig. Int Arc Allergy Appl Immunol, 87, 225-229.
  • Bancroft JD, Cook HC (1984): Manual of Histological Techniques. Churchill Livingstone, London.
  • Banks WJ (1986): Applied Veterinary Histology, pp: 86- 87, Second Edition, Williams and Wilkins Co Baltimore.
  • Barett KE, Metcalfe DD (1987): Heterogeneity of mast cells in the tissues of the respiratory tract and other organ systems. Am Rev Resp Dis, 135, 1190-1195.
  • Becker AB, Chung KF (1985): Mast cells heterogeneity in dog skin. Anat Record, 213, 477-480.
  • Chen W, Alley MR, Manktelow BW, Davey P (1990a): Mast cells in the ovine lower respiratory tract: Heterogeneity, morphology and density. Int Archs Allergy Appl Immunol, 93, 99-106.
  • Chen W, Alley MR, Manktelow BW, Slack P (1990b): Mast cells in the bovine lower respiratory tract: morphology, density and distribution. Brit Vet J, 146, 425- 436.
  • Enerback L (1966b): Mast cells in the rat gastrointestinal mucosa: I. Effects of fixation. Acta Path Microb Scandinav, 66, 298-302.
  • Enerback L (1966a): Mast cells in the rat gastrointestinal mucosa II. Dye-binding and metachromatic properties. Acta Path Microb Scandinav, 66, 303-312.
  • Eren Ü, Aştı RN, Kurtdede N, Sandıkçı M, Sur E (1999): İnek uterusunda mast hücrelerinin histolojik ve histokimyasal özellikleri ve mast hücre heterojenitesi. Tr J Vet Anim Sci, 23, 193-201.
  • Fox B, Bull TB, Guz A (1981): Mast cells in the human alveolar wall: an electron microscopic study. J Clin Pathol, 34, 1333-1342.
  • Fritz FJ, Pabst R (1989): Numbers and heterogeneity of mast cells in the genital tract of the rat. Int Arch Allergy Appl Immunol, 88, 360-362.
  • Galli SJ (1990): New insights in to “The ridle of mast cells” micro environmental regulation of mast cell development and phenotypic heterogeneity. Lab Invest, 62, 5-33.
  • Guerzon GM, Pare PD, Michoud MC, Hogg JC (1979): The number and distribution of mast cells in monkey lungs. Am Rev Respir Dis, 119, 59-66.
  • Güre A (1993): Van Kedisi. In, Abdulkadiroğlu A, Yiğit M, Oğuzbaşaran B (Eds): Van Kütüğü. No: 8, Yüzüncü Yıl Üniversitesi, Van, pp. 722-726.
  • Junquiera LC, Carneiro J (2003): Temel Histoloji (Basic Histology). Çeviri Editörü: Y. Aytekin, S. Solakoğlu. pp: 101-102, Nobel Tıp Kitapevleri.
  • Karaca T (2003): Tavuk ve bıldırcınlarda sindirim sisteminde bulunan mast hücrelerinin dağılımı ve heterojenitesi araştırmalar. Doktora Tezi. YYÜ Sağlık Bilimleri Enstitüsü, Van. morfolojik ve histometrik
  • Karaca T, Yörük M, Şimşek N (2006): Age related distribution of mast cells in the trachea and lung of chicken. Indian Vet J, 83, 649-651.
  • Katz HR, Stewens RL, Austen KF (1985): Heterogeneity of mammalian mast cells differentiated in vivo and in vitro. J Allergy Clin Immunol, 76, 250-259.
  • Kitamura Y, Kanakura Y, Sonoda S, Asai H, Nakano T (1987): Mutual phenotypic changes between connective tissue type and mucosal mast cells. Int Arc Allergy Applied Immun, 82, 244-248.
  • Koçak-Harem M (2001): Tavukların alt solunum yollarındaki mast hücreleri üzerine histolojik araştırmalar. Doktora Tezi. AÜ Sağlık Bilimleri Enstitüsü, Ankara.
  • Kurtdede N, Aştı RN, Ergün L, Ergün E (2000): Ankara keçilerinin alt solunum yolları mast hücreleri üzerine histolojik çalışmalar. AÜ Vet Fak Derg, 47, 339-349.
  • Küther K, Audige L, Kube P, Welle M (1998): Bovine mast cells: distribution, density, heterogeneity and influence of fixation techniques. Cell Tissue Res, 293, 111-119.
  • Mair TS, Stokes CR, Bourne FJ (1988): Distribution and ultrastructure of mast cells in the equine respiratory tract. Equi Vet J, 20, 54-58.
  • Majeed SK (1994): Mast cell distribution in rats. Drug Res, 44, 3.
  • Overveld FJV, Houben LAMJ, Schmitz FEM, Bruijnzeel PLB, Raaijmakers JAM, Terpstra GK (1989): Mast cells heterogeneity in human lung tissue. Clin Sci, 77, 297-304.
  • Özen A, Bayraktaroğlu AG, Ertuğrul T, Özcan Z, Ceylan A, Özen D (2014): Domuz oviduktunda mast hücreleri üzerinde ışık ve electron mikroskobik çalışmalar. Ankara Üniv Vet Fak Derg, 61, 9-14.
  • Özen A, Ergün L, Ergün E, Şimşek N (2007): Morphological studies on ovarian mast cells in the cow. Turk J Vet Anim Sci, 31, 131-136.
  • Pesci A, Rossi GA, Bertorelli G, Aufiero A, Zanon P, Olivieri D (1994): Mast cells in the airway lumen and bronchial mucosa of patients with chronic bronchitis. Am J Resp Crit Care Med, 149, 1311-1316.
  • Schwartz LB, Austen KF (1984): Structure and function of the chemical mediators of mast cells. Prog Allergy (Karger Basel), 34, 271-321.
  • Shanahan F, Niven IM, Dyck N, Denburg JA, Binenstock J, Befus AD (1987): Human lung mast cells: distribution and abundance of histochemically distinct subpopulations. Int Archs Allergy Appl Immun, 83, 329- 331.
  • Tung W (1991): Mast cells in the chick digestive tract II. fixation, distribution, histochemistry and ultrastructure. Tokai J Exp Clin Med, 16, 27-32.
  • Uslu S, Yörük M (2013): Yerli ördek (Anas Platyrhynchase) ve kaz’ın (Anser anser) alt solunum yolları ve akciğerlerinde bulunan mast hücrelerinin dağılımı ve heterojenitesi üzerine morfolojik ve histometrik araştırmalar. Kafkas Univ Vet Fak Derg, 19, 475-482.
  • Warton JM, Papadimitriou RG, Goldie RG, Paterson JW (1986): An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung. Aust J Exp Biol Med Sci, 64, 435-44.
  • Wilkes LK, McMenamin C, Holt PG (1991): Postnatal maturation of mast cell subpopulations in the rat respiratory tract. Immunology, 75, 535-541.
  • Xaubet A, Moises JA, Agusti C, Martos JA, Picado C (1991): Identification of mast cells in bronchoalveolar lavage fluid. Allergy, 46, 222-227. Geliş tarihi: 30.04.2014/ Kabul tarihi: 02.07.2014 Address for correspondence: Dr. Sema Uslu Yüzüncü Yıl University, Faculty of Veterinary Sciences, Departments of Histology and Embryology, 65080 Kampus / VAN - TURKEY e-mail: semauslu43@hotmail.com
There are 36 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA22KF62FC
Journal Section Research Article
Authors

Sema Uslu

Mecit Yörük

Publication Date June 1, 2015
Published in Issue Year 2015Volume: 62 Issue: 2

Cite

APA Uslu, S., & Yörük, M. (2015). A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 62(2), 87-91. https://doi.org/10.1501/Vetfak_0000002663
AMA Uslu S, Yörük M. A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats. Ankara Univ Vet Fak Derg. June 2015;62(2):87-91. doi:10.1501/Vetfak_0000002663
Chicago Uslu, Sema, and Mecit Yörük. “A Morphological and Histometric Study on the Distribution and Heterogeneity of Mast Cells Found in Lungs and Trachea of Van Cats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62, no. 2 (June 2015): 87-91. https://doi.org/10.1501/Vetfak_0000002663.
EndNote Uslu S, Yörük M (June 1, 2015) A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62 2 87–91.
IEEE S. Uslu and M. Yörük, “A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats”, Ankara Univ Vet Fak Derg, vol. 62, no. 2, pp. 87–91, 2015, doi: 10.1501/Vetfak_0000002663.
ISNAD Uslu, Sema - Yörük, Mecit. “A Morphological and Histometric Study on the Distribution and Heterogeneity of Mast Cells Found in Lungs and Trachea of Van Cats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62/2 (June 2015), 87-91. https://doi.org/10.1501/Vetfak_0000002663.
JAMA Uslu S, Yörük M. A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats. Ankara Univ Vet Fak Derg. 2015;62:87–91.
MLA Uslu, Sema and Mecit Yörük. “A Morphological and Histometric Study on the Distribution and Heterogeneity of Mast Cells Found in Lungs and Trachea of Van Cats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 62, no. 2, 2015, pp. 87-91, doi:10.1501/Vetfak_0000002663.
Vancouver Uslu S, Yörük M. A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats. Ankara Univ Vet Fak Derg. 2015;62(2):87-91.

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