Letter to Editor
Year 2015,
Volume: 62 Issue: 1, 51 - 55, 01.03.2015
Abstract
Epididymal sperm may be available as a source of spermatozoa for artificial insemination and in vitro embryo production in many species. The aim of the study was to investigate epididymal sperm freezing on spermatological parameters of Anatolian buffaloes and in vitro evaluation of frozen epididymal sperm for assisted reproductive biotechnologies. The testes and epididymides were collected from 10 Anatolian buffalo bulls (about 3-4 years of age) in Samsun Province. The testes and epididymides from each bull were removed immediately after slaughtering and transported to the laboratory on ice (+4 °C) within 40 min for further processing. After morphological measurements of testes and epididymides were determined, the spermatozoa were collected from cauda epididymides. The percentage of sperm progressive motility and abnormal spermatozoa of fresh and post-thaw sperm samples were evaluated. There was no significant difference between fresh and frozen-thawed sperm samples for progressive sperm motility and head-acrosome anomalies of spermatozoa. The middle part (P<0.001), tail (P<0.001) and total (P<0.001) abnormal spermatozoa rates of frozen-thawed sperm samples were higher than those of fresh sperm samples. In conclusion, collected spermatozoa from cauda epididymides of Anatolian buffalo bull may be used as fresh or frozen for artificial insemination, in vitro embryo production or intra cytoplasmic sperm injection
References
- Akhter S, Ansari MS, Rakha BA, Andrabi SMH, Iqbal S, Ullah N (2010): Cryopreservation of buffalo (Bubalus Bubalis) semen in Bioxcell® extender. Theriogenology, 74: 951-955.
- Andrabi SMH (2009): Factors affecting the quality of cryopreserved buffalo (Bubalus Bubalis) bull spermatozoa. Reprod Domest Anim, 44: 552-569.
- Andrabi SMH, Ansari MS, Ullah N, Anwar M, Mehmood A, Akhter S (2008): Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Anim Reprod Sci, 104: 427-433.
- Annett H (2012): Artificial Insemination in Veterinary Science. In: A Bird's-Eye View of Veterinary Medicine (Carlos C. Perez-Marin ed). Available from: http://www.intechopen.com/books/a-bird-s-eye-view-of- veterinary-medicine/artificial-insemination-in- veterinaryscience.
- Arrighi S, Bosi G, Groppetti D, Cremonesi F (2010): Morpho- and histometric evaluations on the testis and epididymis in buffalo bulls during the different reproductive seasons. The Open Anatomy Journal, 2: 29- 33.
- Barati F, Khaksary MM, Mohammadi GH (2009): Cryopreservation of in situ cool stored buffalo (Bubalus Bubalis) epididymal sperm. Iranian Journal of Veterinary Research, 10: 339-345.
- Bearden HJ, Fuquay JW (1997): Applied animal reproduction. 4th Ed, Prentice Hall, Upper Saddle, New Jersey, 133-177.
- Bruemmer JE (2006): Collection and freezing of epididymal stallion sperm. Vet Clin N Am-Equine, 22: 677-682.
- FAO: Food and Agricultural Organizaion of the United Nations http://faostat.fao.org/site/573/DesktopDefault. aspx?PageID=573#ancor Date of Access: 21.01.2012
- Feradis AH, Pawitri D, Suatha IK, Amin MR, Yusuf TL, Sajuthi D, Budiarsa IN, Hayes ES (2001): Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis). J Med Primatol, 30: 100-106.
- Goovaerts IGF, Hoflack GG, Van Soom A, Dewulf J, Nichi M, Kruif A, Bols PEJ (2006): Evaluation of epididymal semen quality using the Hamilton–Thorne Analyser indicates variation between the two caudae epididymides of the same bull. Theriogenology, 66: 323– 330.
- Graham JK (1994): Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during cryopreservation process. Theriogenelogy, 46: 1151-1162.
- Herold FC, Aurich JE, Gerber D (2004a): Epididymal sperm from the African Buffalo (Syncerus Caffer) can be frozen successfully with Andromed®and with TriladylTM but the addition of bovine seminal plasma is detrimental. Theriogenology, 61: 715-724.
- Herold FC, De Haas K, Cooper D, Colenbrander B, Nothling JO, Theunisen W, Spillings B, Gerber D (2004b): Comparison of three different media for freezing of epididymal sperm from the African buffalo (Syncerus caffer) and influence of equilibration time on the post-thaw sperm quality. Onderstepoort Journal of Veterinary Research, 71: 203-201.
- Herold FC, de Haas K, Colenbrander B, Gerber D (2006): Comparison of equilibration times when freezing epididymal sperm from African buffalo (Syncerus caffer) using TriladylTM or AndroMed®. Theriogenology, 66: 1123-1130.
- Javed MT, Khan A, Naz NA (2001): Studies on abnormal buffalo bulls with reference to scrotal circumference, semen characteristics, seminal plasma hormones and their association with testicular and epididymal histopathology. Vet. Arhiv,71: 223-236.
- Lalthazuali, Sing J, Ghuman SPS, Pandey AK, Uppal SK, Ahuja CS, Dhaliwa GS (2010): Effect of insulin treatment during the post-ai mid-luteal phase on the blood biochemical profile and conception rate in buffaloes. Buffalo Bulletin Vol.29 No.4
- Lambrechts H, Van Niekerk FE, Coetzer WA, Cloete SWP, Van Der Horst G (1999): The effect of cryopreservation on the survivabilit, viability and motility of epididymal African buffalo (Syncerus caffer) spermatozoa. Theriogenelogy, 52: 1241-1249.
- Lessard C, Danielson J, Rajapaksha K, Adams GP, McCorkell R (2009): Banking North American buffalo semen. Theriogenology, 71: 1112-1119.
- Nazir G, Ghuman SPS, Singh J, Honparkhe M, Ahuja CS, Dhaliwal GS, Sangha MK, Saijpaul S, Agarwal SK (2013): Improvement of conception rate in postpartum flaxseed supplemented buffalo with Ovsynch + CIDR protocol. Animal Reproduction Science, 137: 15–22.
- Papa FO, Melo CM, Fioratti EG, Dell’Aqua Jr JA, Zahn FS, Alvarenga MA (2008): Freezing of stallion epididymal sperm. Anim Reprod Sci, 107: 293-301.
- Perera BMAO (1999): Reproduction in water buffalo: comparative aspects and implications for management. J Reprod Fertil Suppl 54: 157–168.
- Raizada BC, Sattar A, Pandey MD 1990: A comparative study of freezing buffalo semen in two diluters.In: Acharya, R.M., Lokeshwar, R.R., Kumar, S._Eds.., Recent Advances in Buffalo Research vol. 3 pp. 66–74.
- Rasul Z, Anzar M, Jalali S, Ahmad N (2000): Effect of buffering systems on post-thaw motion characteristics, plasma membrane integrity and acrosome morphology of buffalo spermatozoa. Anim Reprod Sci, 59: 31-41.
- Rizal M (2006): The fertility of frozen-thawed ejaculate vs epididymal Garut ram sperm. Veterinary Sains J, 24: 49- 57.
- SAS (2009): SAS Statistical sofware. SAS Campus Drive, Cary, NC, 27513, USA.
- Sharma M, Singh M, Kapoor S, Jasial S (2012): Inter relationship between some routine semen evaluation parameters in Jersey X local hill cattle crossbred bulls. Open Veterinary Journal, 2: 26-31.
- Snedecor W, Cochran WG (1980): Statistical Methods. 7th ed, The Iowa state University Press, Ames, Iowa, USA.
- Soysal MI,Kok S,Gurcan EK (2005): An investigation on the distribution in erythrocytes potassium polymorphisms in buffaloes. JOTAF, 2: 189-193.
- Tekin N (1994): Spermanın muayenesi ve değerlendirilmesi. In: Evcil Hayvanlarda Reprodüksiyon, Sun’i Tohumlama, Doğum ve İnfertilite (Alaçam A, ed). Dizgievi, Konya, Turkey, pp: 67-79.
- Vale WG (1997): Sperm cryopreservation. Bubalus bubalis, 4: 129-140.
Anadolu mandalarında testiküler morfoloji ve dondurulmuş epididimal spermanın in vitrodeğerlendirilmesi
Abstract
Epididimal sperma birçok türde suni tohumlama ve in vitro embriyo üretiminde spermatozoa kaynağı olarak kullanılabilir. Yapılan bu çalışmanın amacı Anadolu Mandaları’ndan alınan epididimal spermanın dondurulmasının bazı spermatolojik parametreler üzerine etkilerinin incelenmesi ve yardımcı üreme teknolojileri için dondurulan epididimal spermanın kullanılabilirliğinin in vitro değerlendirilmesidir. Samsun İlinde kesilen (yaklaşık 3-4 yaşlı) toplam 10 adet erkek Anadolu Mandasından kesim sonrasında testisler ve epididimisler hemen alınarak buz içinde (+4 °C) 40 dakika içerisinde daha sonraki işlemler için laboratuara getirildi. Testis ve epididimislerin morfolojik ölçüleri belirlendikten sonra spermatozoa cauda epididimisten toplandı. Nativ ve çözüm sonu örneklerde progresif spermatozoa motilitesi ve anormal spermatozoa yüzde oranları değerlendirildi. Nativ ve dondurulmuş çözülmüş sperma örnekleri arasında progresif spermatozoa motilitesi ve spermatozoa baş-akrozom anomalileri yönünden herhangi bir fark tespit edilmedi. Dondurulmuş çözülmüş spermadaki orta kısım (P<0,001), kuyruk (P<0,001) ve total (P<0,001) anormal spermatozoa oranları, nativ spermaya göre daha yüksek bulunmuştur. Sonuç olarak, Anadolu Mandasının cauda epididimisinden elde edilen spermatozoa nativ veya dondurulmuş epididimal sperma olarak suni tohumlamada, in vitro embriyo üretiminde ya da intra sitoplazmik spermatozoa enjeksiyonu gibi yardımcı üreme biyoteknolojilerinde kullanılabilir
References
- Akhter S, Ansari MS, Rakha BA, Andrabi SMH, Iqbal S, Ullah N (2010): Cryopreservation of buffalo (Bubalus Bubalis) semen in Bioxcell® extender. Theriogenology, 74: 951-955.
- Andrabi SMH (2009): Factors affecting the quality of cryopreserved buffalo (Bubalus Bubalis) bull spermatozoa. Reprod Domest Anim, 44: 552-569.
- Andrabi SMH, Ansari MS, Ullah N, Anwar M, Mehmood A, Akhter S (2008): Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Anim Reprod Sci, 104: 427-433.
- Annett H (2012): Artificial Insemination in Veterinary Science. In: A Bird's-Eye View of Veterinary Medicine (Carlos C. Perez-Marin ed). Available from: http://www.intechopen.com/books/a-bird-s-eye-view-of- veterinary-medicine/artificial-insemination-in- veterinaryscience.
- Arrighi S, Bosi G, Groppetti D, Cremonesi F (2010): Morpho- and histometric evaluations on the testis and epididymis in buffalo bulls during the different reproductive seasons. The Open Anatomy Journal, 2: 29- 33.
- Barati F, Khaksary MM, Mohammadi GH (2009): Cryopreservation of in situ cool stored buffalo (Bubalus Bubalis) epididymal sperm. Iranian Journal of Veterinary Research, 10: 339-345.
- Bearden HJ, Fuquay JW (1997): Applied animal reproduction. 4th Ed, Prentice Hall, Upper Saddle, New Jersey, 133-177.
- Bruemmer JE (2006): Collection and freezing of epididymal stallion sperm. Vet Clin N Am-Equine, 22: 677-682.
- FAO: Food and Agricultural Organizaion of the United Nations http://faostat.fao.org/site/573/DesktopDefault. aspx?PageID=573#ancor Date of Access: 21.01.2012
- Feradis AH, Pawitri D, Suatha IK, Amin MR, Yusuf TL, Sajuthi D, Budiarsa IN, Hayes ES (2001): Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis). J Med Primatol, 30: 100-106.
- Goovaerts IGF, Hoflack GG, Van Soom A, Dewulf J, Nichi M, Kruif A, Bols PEJ (2006): Evaluation of epididymal semen quality using the Hamilton–Thorne Analyser indicates variation between the two caudae epididymides of the same bull. Theriogenology, 66: 323– 330.
- Graham JK (1994): Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during cryopreservation process. Theriogenelogy, 46: 1151-1162.
- Herold FC, Aurich JE, Gerber D (2004a): Epididymal sperm from the African Buffalo (Syncerus Caffer) can be frozen successfully with Andromed®and with TriladylTM but the addition of bovine seminal plasma is detrimental. Theriogenology, 61: 715-724.
- Herold FC, De Haas K, Cooper D, Colenbrander B, Nothling JO, Theunisen W, Spillings B, Gerber D (2004b): Comparison of three different media for freezing of epididymal sperm from the African buffalo (Syncerus caffer) and influence of equilibration time on the post-thaw sperm quality. Onderstepoort Journal of Veterinary Research, 71: 203-201.
- Herold FC, de Haas K, Colenbrander B, Gerber D (2006): Comparison of equilibration times when freezing epididymal sperm from African buffalo (Syncerus caffer) using TriladylTM or AndroMed®. Theriogenology, 66: 1123-1130.
- Javed MT, Khan A, Naz NA (2001): Studies on abnormal buffalo bulls with reference to scrotal circumference, semen characteristics, seminal plasma hormones and their association with testicular and epididymal histopathology. Vet. Arhiv,71: 223-236.
- Lalthazuali, Sing J, Ghuman SPS, Pandey AK, Uppal SK, Ahuja CS, Dhaliwa GS (2010): Effect of insulin treatment during the post-ai mid-luteal phase on the blood biochemical profile and conception rate in buffaloes. Buffalo Bulletin Vol.29 No.4
- Lambrechts H, Van Niekerk FE, Coetzer WA, Cloete SWP, Van Der Horst G (1999): The effect of cryopreservation on the survivabilit, viability and motility of epididymal African buffalo (Syncerus caffer) spermatozoa. Theriogenelogy, 52: 1241-1249.
- Lessard C, Danielson J, Rajapaksha K, Adams GP, McCorkell R (2009): Banking North American buffalo semen. Theriogenology, 71: 1112-1119.
- Nazir G, Ghuman SPS, Singh J, Honparkhe M, Ahuja CS, Dhaliwal GS, Sangha MK, Saijpaul S, Agarwal SK (2013): Improvement of conception rate in postpartum flaxseed supplemented buffalo with Ovsynch + CIDR protocol. Animal Reproduction Science, 137: 15–22.
- Papa FO, Melo CM, Fioratti EG, Dell’Aqua Jr JA, Zahn FS, Alvarenga MA (2008): Freezing of stallion epididymal sperm. Anim Reprod Sci, 107: 293-301.
- Perera BMAO (1999): Reproduction in water buffalo: comparative aspects and implications for management. J Reprod Fertil Suppl 54: 157–168.
- Raizada BC, Sattar A, Pandey MD 1990: A comparative study of freezing buffalo semen in two diluters.In: Acharya, R.M., Lokeshwar, R.R., Kumar, S._Eds.., Recent Advances in Buffalo Research vol. 3 pp. 66–74.
- Rasul Z, Anzar M, Jalali S, Ahmad N (2000): Effect of buffering systems on post-thaw motion characteristics, plasma membrane integrity and acrosome morphology of buffalo spermatozoa. Anim Reprod Sci, 59: 31-41.
- Rizal M (2006): The fertility of frozen-thawed ejaculate vs epididymal Garut ram sperm. Veterinary Sains J, 24: 49- 57.
- SAS (2009): SAS Statistical sofware. SAS Campus Drive, Cary, NC, 27513, USA.
- Sharma M, Singh M, Kapoor S, Jasial S (2012): Inter relationship between some routine semen evaluation parameters in Jersey X local hill cattle crossbred bulls. Open Veterinary Journal, 2: 26-31.
- Snedecor W, Cochran WG (1980): Statistical Methods. 7th ed, The Iowa state University Press, Ames, Iowa, USA.
- Soysal MI,Kok S,Gurcan EK (2005): An investigation on the distribution in erythrocytes potassium polymorphisms in buffaloes. JOTAF, 2: 189-193.
- Tekin N (1994): Spermanın muayenesi ve değerlendirilmesi. In: Evcil Hayvanlarda Reprodüksiyon, Sun’i Tohumlama, Doğum ve İnfertilite (Alaçam A, ed). Dizgievi, Konya, Turkey, pp: 67-79.
- Vale WG (1997): Sperm cryopreservation. Bubalus bubalis, 4: 129-140.
There are 31 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA32HY47AK |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2015 |
| Published in Issue | Year 2015Volume: 62 Issue: 1 |