Akdeniz’de yakalanan Dülger balığındaki Anisakis pegreffii (Nematoda: Anisakidae) enfeksiyonununmoleküler identifikasyonu ve patolojisi
Abstract
Çalışmanın materyalini Akdeniz’de Antalya körfezi avlanma sahasında yakalanan bir dülger balığı Zeus faber (Linnaeus, 1758) oluşturdu. Zeus faber dikkatli bir şekilde diseke edildi ve karın boşluğu, karaciğer ve kaslar Anisakidae ailesindeki nematod larvaları yönünden incelendi. Makroskopik olarak karın boşluğunda sekiz larva ile karaciğer yüzeyinde kapsüle olmuş 7 larva bulundu. Makroskobik incelemede, solgun görünümde olan karaciğer yüzeyinde kanamalara ve kapsül altında 0,3 cm çapında kistlere rastlandı. Histopatolojik incelemede, hepatositlerde şiddetli dejenerasyon ve nekroz tanımlandı. Karaciğer kapsülü altında fibröz kapsülle çevrili Anisakis larvaları gözlendi. Morfolojik olarak tanımlanan Anisakis Tip I larvalar tür teşhisi için PZR-RFLP ile moleküler olarak incelendi. Tüm Anisakis larvalar PZR-RFLP ile Anisakis pegreffii olarak teşhis edildi. Bu çalışma ile Türkiye sularında avlanan dülger balığında Anisakis pegreffii’nin moleküler identifikasyonu ve paratenik ya da ara konakdaki patolojik bulguları ilk kez tanımlandı
Keywords
References
- Akmırza A (2013): Gökçeada kıyı sularındaki balıkların parazitik nematodları. T Parazitol Derg, 37, 199-202.
- Berland B (1961): Nematodes from some Norwegian marine fishes. Sarsia, 2, 1-50.
- Brian L (1958): Ricerche sul ciclo biologico e l’ecologia di Ascaris capsularia Rud. Atti dell‘Accademia ligure di scienze e lettere, 14, 249-263.
- D'Amelio S, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanco M, Paggi L (2000): Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. Int J Parasitol, 30, 223–226.
- Elarifi AE (1982): The histopathology of larval anisakid nematode infections in the liver of whiting, Merlangius merlangus (L.), with some observations on blood leucocytes of the fish. J Fish Dis, 5, 411-419.
- Guiart J (1938): Étude parasitologique et épidémiologique de quelques poisons de mer. Bulletin de l’lnstitut océanographique de Monaco, No. 755, 15 pp.
- Janssen GM (1979): The occurrence of Zeus faber (Linnaeus, 1758) in the coastal waters of the Netherlands (Pisces, Zeiformaes). Bull Zool Mus Univ Amsterdam, 6, 153–158.
- Kahl W (1938): Nematoden in Seefischen. 11. Erhebungen uber den Befall von Seefischen mit Larven von Anacanthocheilus rotundatus (Rudolphi) und die durch diese Larven hervorgerufenen Reaktionen des Wirtsgewebes. Zeitschrift fur Parasitenkunde, 10, 513-534.
- Mattiucci S, Nascetti G (2008): Advances and trends in the molecular systematics of Anisakis nematodes, with implications for their evolutionary ecology and host- parasite co-evolutionary processes. Adv Parasitol, 66, 47–148.
- Mattiucci S, Paoletti M, Borrini F, Palumbo M, Palmieri RM, Gomes V, Casati A, Nascetti G (2011): First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in a paraffin- embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect Dis, 31, 11–82.
- Quiazon KMA, Yoshinaga T, Ogava K, Yukami R (2008): Morphological differences between larvae and in vitro cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae). Parasitol Int, 57, 483–489.
- Pontes T, D’Amelio S, Costa G, Paggi L (2005): Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. J Parasitol, 91, 1430–1434.
- Prusevich TO (1964): On the formation of capsules around larvae of Anisakis sp. in the tissues of the shorthorn sculpin Myoxocephalus scorpius. Trudy Murmanskogo Biologicheskogo Instituta, 5, 265-273. (In Russian)
- Remotti E (1933): Ancora sull’ Ascaris capsularia Rud. Bollettino dei Musei e laboratorii di zoologia e di anatomia comparata della R. Universit di Genova, 68, 1-15.
- Smith,JW, Wootten R (1978): Anisakis and anisakiasis. Adv Parasitol, 16, 93–163.
- Tepe Y, Oguz MC(2013): Nematode and acanthocephalan parasites of marine fish of the eastern Black Sea coasts of Turkey. Turk J Zool, 37, 753–760.
- Utuk AE, Pişkin FÇ, Balkaya İ (2012): Molecular detection of Anisakis pegreffii in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Univ Vet Fak Derg, 18, 303–307.
- Zhu XQ, Gasser, RB, Podolska M, Chilton N (1998): Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol, 28, 1911–1921.
Pathology and molecular identification of Anisakis pegreffii (Nematoda: Anisakidae) infection in the John Dory, Zeus faber (Linnaeus, 1758) caught in Mediterranean Sea
Abstract
One specimen of John Dory, Zeus faber (Linnaeus, 1758), was caught from commercial sites at the costs of Gulf of Antalya at Mediterranean Sea, Turkey. Zeus faber was dissected carefully. After examination Anisakidae larvae were recovered from in the abdominal cavity, liver and muscles. A total of eight larvae were found in the abdominal cavity and seven larvae encapsulated on surface of liver at necropsy. In the macroscopic examination, liver was seen to pale and surface of the capsula was haemorhagic with subcapsular cysts including larvae in 0.3 cm diameter. In the histopathologic examination severe degeneration and hepatic necrosis was identified. Just below the liver capsula, Anisakis larvae were seen covered with fibrous capsula. The larvae morphologically identified as Anisakis larvae Type I were subjected to further molecular characterization by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify the species. All Anisakis larvae were identified at species level by PCR-RFLP as belonging to A. pegreffii. Within the present study, for the first time, we report on the molecular identification of A. pegreffii larvae from John Dory, Z. faber, and we describe their pathological significance in a paratenic or intermediate hosts in Turkish waters
References
- Akmırza A (2013): Gökçeada kıyı sularındaki balıkların parazitik nematodları. T Parazitol Derg, 37, 199-202.
- Berland B (1961): Nematodes from some Norwegian marine fishes. Sarsia, 2, 1-50.
- Brian L (1958): Ricerche sul ciclo biologico e l’ecologia di Ascaris capsularia Rud. Atti dell‘Accademia ligure di scienze e lettere, 14, 249-263.
- D'Amelio S, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanco M, Paggi L (2000): Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. Int J Parasitol, 30, 223–226.
- Elarifi AE (1982): The histopathology of larval anisakid nematode infections in the liver of whiting, Merlangius merlangus (L.), with some observations on blood leucocytes of the fish. J Fish Dis, 5, 411-419.
- Guiart J (1938): Étude parasitologique et épidémiologique de quelques poisons de mer. Bulletin de l’lnstitut océanographique de Monaco, No. 755, 15 pp.
- Janssen GM (1979): The occurrence of Zeus faber (Linnaeus, 1758) in the coastal waters of the Netherlands (Pisces, Zeiformaes). Bull Zool Mus Univ Amsterdam, 6, 153–158.
- Kahl W (1938): Nematoden in Seefischen. 11. Erhebungen uber den Befall von Seefischen mit Larven von Anacanthocheilus rotundatus (Rudolphi) und die durch diese Larven hervorgerufenen Reaktionen des Wirtsgewebes. Zeitschrift fur Parasitenkunde, 10, 513-534.
- Mattiucci S, Nascetti G (2008): Advances and trends in the molecular systematics of Anisakis nematodes, with implications for their evolutionary ecology and host- parasite co-evolutionary processes. Adv Parasitol, 66, 47–148.
- Mattiucci S, Paoletti M, Borrini F, Palumbo M, Palmieri RM, Gomes V, Casati A, Nascetti G (2011): First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in a paraffin- embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect Dis, 31, 11–82.
- Quiazon KMA, Yoshinaga T, Ogava K, Yukami R (2008): Morphological differences between larvae and in vitro cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae). Parasitol Int, 57, 483–489.
- Pontes T, D’Amelio S, Costa G, Paggi L (2005): Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. J Parasitol, 91, 1430–1434.
- Prusevich TO (1964): On the formation of capsules around larvae of Anisakis sp. in the tissues of the shorthorn sculpin Myoxocephalus scorpius. Trudy Murmanskogo Biologicheskogo Instituta, 5, 265-273. (In Russian)
- Remotti E (1933): Ancora sull’ Ascaris capsularia Rud. Bollettino dei Musei e laboratorii di zoologia e di anatomia comparata della R. Universit di Genova, 68, 1-15.
- Smith,JW, Wootten R (1978): Anisakis and anisakiasis. Adv Parasitol, 16, 93–163.
- Tepe Y, Oguz MC(2013): Nematode and acanthocephalan parasites of marine fish of the eastern Black Sea coasts of Turkey. Turk J Zool, 37, 753–760.
- Utuk AE, Pişkin FÇ, Balkaya İ (2012): Molecular detection of Anisakis pegreffii in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Univ Vet Fak Derg, 18, 303–307.
- Zhu XQ, Gasser, RB, Podolska M, Chilton N (1998): Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol, 28, 1911–1921.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA94SV27MD |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2014 |
| Published in Issue | Year 2014Volume: 61 Issue: 3 |