The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra
Abstract
In this study, rabbits were used as a model for canine pyometra to test the effects of preoperative therapy in the reduction of postoperative stress. Pyometra were induced in 18 healthy rabbits by the operative administration of Pasteurella multocida inoculum. Beginning on the third day after inoculation Group I (n=6) received enrofloxacin (2,5 mg/kg, s.c.), Group II (n=6) recieved enrofloxacin (2,5 mg/kg, s.c.) + vitamin C (100 mg, s.c.) injections for three days. Ovariohysterectomy were performed in groups I and II following this medical treatment. Group III (n=6) as the control group, did not recieve any medical therapy and were operated on the third day after inoculation. Blood samples were collected before inoculation, on the day of operation, 24th h, 72th h and at 7th day following the operations. Ceruloplasmin (CERU), malondialdehyde (MDA), vitamin C, haptoglobin (Hp) and serum amyloid-A (SAA) levels were analyzed. In the postoperative 72th h period mean CERU level of Group III were found significantly higher (p<0,05) than Group I and II. Mean MDA level were significantly higher in Group III on the day of operation compared to the treatment groups (p<0,05). SAA levels rised in all of the groups following inoculation, on the day of operation (p<0,05) which is followed by a gradual decline until the 7th day. Overall CERU and MDA levels tend to be higher in the control group compared to the treatment groups whereas Hp and SAA levels were not significantly different. These findings may indicate that preoperative therapy may reduce the oxidative stress mildly however it did not significantly alter the acute phase protein response definitively and a significant positive effect of both preoperative treatments compared to the control group could not be pronounced
References
- Chan JP, Chang C, Hsu W, Liu W, Chen T (2010): Association of increased serum acute-phase protein concentrations with reproductive performance in dairy cows with postpartum metritis. Vet Clin Pathol, 39, 72 – 78.
- Chen YMM, Wright PJ, Lee CS (2001): A model for the study of cystic endometrial hyperplasia in bitches. J Reprod Fertil Suppl, 57, 407 – 414.
- Cray C, Zaias J, Altman NH (2009): Acute phase response in animals: a review. Comp Med, 59, 517 - 526.
- Dabrowski R, Kostro K, Lisiecka U, Szczubial M, Krakowski L (2009): Usefulness of C-reactive protein, Serum Amyloid A component, and haptoglobin determinations in bitches with pyometra for monitoring early post- ovariohysterectomy complications. Theriogenology, 72, 471 – 476.
- Dabrowski R, Wawron W, Kostro K (2007): Changes in CRP, SAA and haptoglobin produced in response to ovariohysterectomy in healthy bitches and those with pyometra. Theriogenology, 67, 321–327.
- De Bosschere H, Ducatelle R, Tshamala M (2003): Uterine oestrogen and progesterone receptor expression in experimental pyometra in the bitch. J Comp Pathol, 128, 99 – 106.
- Fabro de Bem A, Portella RL, Perottoni J, Becker E, Bohrer D, Paixao MW, Nogueira CW, Zeni G, Rocha JBT (2006): Changes in biochemical parameters in rabbits blood after oral exposure to diphenyl diselenide for long periods. Chem-Biol Interact, 162, 1 – 10.
- Giclas PC, Manthei U, Strunk RC (1985): The acute phase response of C3, C5, ceruloplasmin, and C-reactive protein induced by turpentine pleurisy in the rabbit. Am J Pathol, 120, 146 - 156.
- Huang Y, Xie K, Li J, Xu N, Gong G, Wang G, Yu Y, Dong H, Xiong L (2011): Beneficial effects of hydrogen gas against spinal cord ischemia-reperfusion injury in rabbits. Brain Res, 1378, 125 – 136.
- Johnson JH, Wolf AM (1983): Ovarian abscesses and pyometra in a domestic rabbit. JAVMA, 203, 667-669.
- Karabulut AB, Gül M, Karabulut E, Kıran TR, Ocak SG, Otlu O (2010): Oxidant and antioxidant activity in rabbit livers treated with zoledronic acid. Transplant Proc, 42, 3820 - 3822.
- Karakılçık AZ, Zerin M, Arslan O, Nazlıgül Y, Vural H (2004): Effects of vitamin C and E on liver enzymes and biochemical parameters of rabbits exposed to aflatoxin B1. Vet Hum Toxicol, 46, 190 - 192.
- Kway A (1987): A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta, 86, 153.
- Lin Y, Xue ES, Liang RX, Huang WQ, Yu L, Chen S, Yu LY, Lin LW (2010): Ultrasonographic appearance of experimental testicular ischemia and protective effect of allopurinol. Zhonghua Nan Ke Xue, 16, 1083 - 8.
- Luck MR, Jeyaseelan I, Scholes RA (1995): Ascorbic acid and fertility. Biol Reprod, 52: 262 – 266.
- Mackiewicz A, Ganapthi MK, Schultz D, Samlos D, Reese J, Kushner I (1988): Regulation of rabbit acute phase protein biosynthesis by monokines, Biochem J, 253, 851 - 857.
- McGahan MC (1985): Ascorbic acid levels in aqueous and vitreous humors of the rabbit: effects of inflammation and ceruloplasmin. Exp Eye Res, 41, 291-298.
- Mor F, Özmen O (2010): Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits. Exp Toxicol Pathol, 62, 75 - 80.
- Nomura K, Funahashi H (1999): Histological Characteristics of Canine Deciduoma Induced by Intrauterine Inoculation of E. coli Suspension. J Vet Med Sci, 61, 433–438.
- Paltrinieri S (2008): The feline acute phase reaction. Vet J, 177, 26 - 35.
- Phillips JD, Kim CS, Fonkalsrud EW, Zeng H, Dindar H (1992): Effects of chronic corticosteroids and vitamin A on the healing of intestinal anastomoses. Am J Surg, 163, 71-77.
- Sunderman FW, Nomoto S (1970): Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clinical Chemistry, 16, 903 – 910.
- Voelkel EF, Levine L, Alper CA, Tashjian AH (1978): Acute phase reactants ceruloplasmin and haptoglobin and their relationship to plasma prostaglandins in rabbits bearing the VX2 carcinoma. J Exp Med, 147, 1078 - 1088.
- Winsey K, Simon RJ, Levenson SM, Seifter E, Demetriou AA (1987): Effect of supplemental vitamin A on colon anastomotic healing in rats given preoperative irradiation. Am J Surg, 153, 153-6.
- Xu J, Yunshi Z, Li R (2009): Immunonutrition in surgical patients. Curr Drug Targets, 10, 771-777.
- Yaralıoğlu-Gurgoze S, Çetin H, Cen O, Yılmaz S, ATLI MO (2011): Changes in MDA concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis. Acta Pol Pharm, 68: 15-22.
- Yörük O, Üçüncü H, Sütbeyaz Y, Aktan B, Gür FO (2009): The effects of methylprednisolone and cefazolin sodium on antioxidant status in experimentally induced maxillary sinusitis. Acta Otolaryngol, 129, 1101 - 1105.
Tavşanlarda deneysel oluşturulan pyometra olgularında, preoperatif antibiyotik veya antibiyotik + Cvitamini enjeksiyonlarının yangısal ve oksidatif duruma olan etkileri
Abstract
Bu çalışmada pyometra operasyonu sonrası stresin azaltılmasında preoperatif sağaltımın etkisini araştırmak amacıyla tavşanlar model olarak kullanıldı. Bu amaçla onsekiz sağlıklı tavşanda Pasteurella multocida’ nın operatif yöntemle uterusa inokulasyonu gerçekleştirildi. İnokulasyondan sonra 3. günden başlanarak Grup I’ e (n=6) enrofloksasin (2,5 mg/kg, s.c.), Grup II’ ye (n=6) aynı dozda enrofloksasin ve vitamin C (100 mg, s.c.) enjeksiyonları 3 gün süreyle devam ettirilmiş ve bundan sonra ovaryohisterektomi uygulanmıştır. Kontrol grubu olan Grup III’ e (n=6) medikal sağaltım uygulanmamış ve inokulasyondan sonra üçüncü günde ovaryohisterektomi uygulanmıştır. Kan örnekleri inokulasyondan önce, ovaryohisterektomi gününde, postoperatif 24. saat, 72. saat ve 7. günde alınmıştır. Bu örneklerde serüloplazmin (CERU), malondialdehit (MDA), vitamin C, haptoglobin (Hp) ve Serum Amiloid A (SAA) düzeyleri belirlenmiştir. Serüloplazmin bütün gruplarda inokulasyonu takiben yükselirken Grup I ve II’ de postoperatif 72s’ de düşmüş ancak Grup III’ de yüksek bulunmuştur (p<0,05). Yine Grup II’ de MDA düzeyleri operasyon gününde sağaltım gruplarına göre yüksektir (p<0,05). SAA düzeyleri zamana bağlı olarak bütün gruplarda operasyon gününde yükselmiş daha sonra postoperatif 7. güne kadar düşmüştür. Genel olarak CERU ve MDA düzeylerinin kontrol grubunda diğer gruplara göre daha yüksek olduğu, Hp ve SAA düzeylerinde ise gruplar arasında önemli bir farklılık görülmediği saptanmıştır. Bu bulgular preoperatif sağaltımın oksidatif stresi önlemede sınırlı düzeyde etkili olurken akut faz yanıtı üzerinde önemli düzeyde etkili olmadığını göstermektedir
References
- Chan JP, Chang C, Hsu W, Liu W, Chen T (2010): Association of increased serum acute-phase protein concentrations with reproductive performance in dairy cows with postpartum metritis. Vet Clin Pathol, 39, 72 – 78.
- Chen YMM, Wright PJ, Lee CS (2001): A model for the study of cystic endometrial hyperplasia in bitches. J Reprod Fertil Suppl, 57, 407 – 414.
- Cray C, Zaias J, Altman NH (2009): Acute phase response in animals: a review. Comp Med, 59, 517 - 526.
- Dabrowski R, Kostro K, Lisiecka U, Szczubial M, Krakowski L (2009): Usefulness of C-reactive protein, Serum Amyloid A component, and haptoglobin determinations in bitches with pyometra for monitoring early post- ovariohysterectomy complications. Theriogenology, 72, 471 – 476.
- Dabrowski R, Wawron W, Kostro K (2007): Changes in CRP, SAA and haptoglobin produced in response to ovariohysterectomy in healthy bitches and those with pyometra. Theriogenology, 67, 321–327.
- De Bosschere H, Ducatelle R, Tshamala M (2003): Uterine oestrogen and progesterone receptor expression in experimental pyometra in the bitch. J Comp Pathol, 128, 99 – 106.
- Fabro de Bem A, Portella RL, Perottoni J, Becker E, Bohrer D, Paixao MW, Nogueira CW, Zeni G, Rocha JBT (2006): Changes in biochemical parameters in rabbits blood after oral exposure to diphenyl diselenide for long periods. Chem-Biol Interact, 162, 1 – 10.
- Giclas PC, Manthei U, Strunk RC (1985): The acute phase response of C3, C5, ceruloplasmin, and C-reactive protein induced by turpentine pleurisy in the rabbit. Am J Pathol, 120, 146 - 156.
- Huang Y, Xie K, Li J, Xu N, Gong G, Wang G, Yu Y, Dong H, Xiong L (2011): Beneficial effects of hydrogen gas against spinal cord ischemia-reperfusion injury in rabbits. Brain Res, 1378, 125 – 136.
- Johnson JH, Wolf AM (1983): Ovarian abscesses and pyometra in a domestic rabbit. JAVMA, 203, 667-669.
- Karabulut AB, Gül M, Karabulut E, Kıran TR, Ocak SG, Otlu O (2010): Oxidant and antioxidant activity in rabbit livers treated with zoledronic acid. Transplant Proc, 42, 3820 - 3822.
- Karakılçık AZ, Zerin M, Arslan O, Nazlıgül Y, Vural H (2004): Effects of vitamin C and E on liver enzymes and biochemical parameters of rabbits exposed to aflatoxin B1. Vet Hum Toxicol, 46, 190 - 192.
- Kway A (1987): A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta, 86, 153.
- Lin Y, Xue ES, Liang RX, Huang WQ, Yu L, Chen S, Yu LY, Lin LW (2010): Ultrasonographic appearance of experimental testicular ischemia and protective effect of allopurinol. Zhonghua Nan Ke Xue, 16, 1083 - 8.
- Luck MR, Jeyaseelan I, Scholes RA (1995): Ascorbic acid and fertility. Biol Reprod, 52: 262 – 266.
- Mackiewicz A, Ganapthi MK, Schultz D, Samlos D, Reese J, Kushner I (1988): Regulation of rabbit acute phase protein biosynthesis by monokines, Biochem J, 253, 851 - 857.
- McGahan MC (1985): Ascorbic acid levels in aqueous and vitreous humors of the rabbit: effects of inflammation and ceruloplasmin. Exp Eye Res, 41, 291-298.
- Mor F, Özmen O (2010): Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits. Exp Toxicol Pathol, 62, 75 - 80.
- Nomura K, Funahashi H (1999): Histological Characteristics of Canine Deciduoma Induced by Intrauterine Inoculation of E. coli Suspension. J Vet Med Sci, 61, 433–438.
- Paltrinieri S (2008): The feline acute phase reaction. Vet J, 177, 26 - 35.
- Phillips JD, Kim CS, Fonkalsrud EW, Zeng H, Dindar H (1992): Effects of chronic corticosteroids and vitamin A on the healing of intestinal anastomoses. Am J Surg, 163, 71-77.
- Sunderman FW, Nomoto S (1970): Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clinical Chemistry, 16, 903 – 910.
- Voelkel EF, Levine L, Alper CA, Tashjian AH (1978): Acute phase reactants ceruloplasmin and haptoglobin and their relationship to plasma prostaglandins in rabbits bearing the VX2 carcinoma. J Exp Med, 147, 1078 - 1088.
- Winsey K, Simon RJ, Levenson SM, Seifter E, Demetriou AA (1987): Effect of supplemental vitamin A on colon anastomotic healing in rats given preoperative irradiation. Am J Surg, 153, 153-6.
- Xu J, Yunshi Z, Li R (2009): Immunonutrition in surgical patients. Curr Drug Targets, 10, 771-777.
- Yaralıoğlu-Gurgoze S, Çetin H, Cen O, Yılmaz S, ATLI MO (2011): Changes in MDA concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis. Acta Pol Pharm, 68: 15-22.
- Yörük O, Üçüncü H, Sütbeyaz Y, Aktan B, Gür FO (2009): The effects of methylprednisolone and cefazolin sodium on antioxidant status in experimentally induced maxillary sinusitis. Acta Otolaryngol, 129, 1101 - 1105.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA23FA92EU |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2013 |
| Published in Issue | Year 2013Volume: 60 Issue: 3 |