Letter to Editor
Year 2013,
Volume: 60 Issue: 1, 7 - 13, 01.03.2013
Abstract
The present study was aimed at the light and electron microscopic examination of the epithelium of the porcine oviduct, determination of the histochemical structure of the mucus secreted by epithelial cells, and demonstration of the localisation and distribution of endothelial nitric oxide synthase (eNOS) activity during the different stages of the oestrous cycle. The oviducts of 5 healthy adult sows constituted the material of the study. Histochemically, it was observed that, increased glycosaminoglycan (GAG) secretion during the oestrus phase, was in particular stored in the crypts of the isthmus. Electron microscopic examination revealed that, the increased amount of secretory material, which accumulated in secretory cells during the oestrus phase, was stored in two different types of secretory granules. Immunohistochemically, it was ascertained that eNOS was not expressed in either the ampulla or the fimbria during the oestrus and luteal phases, but was expressed in the isthmus only during the oestrus phase
Keywords
References
- Abe H, Hoshi H (2007): Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig. Reprod Dom Anim, 42, 292-298.
- Abe H, Hoshi H (2008): Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and large white pigs during the oestrous cycle. Reprod Dom Anim, 43,66-73.
- Bancroft J D, Gamble M (2002): Histological Techniques. 5th edition, London, Philadelphia: Churchill & Livingstone, pp.182-189.
- Bullon F, Merchan J A, Gonzalez-Gomez F, Furio V, Poblete EG (1980): Ultrastructure of the oviductal mucosa of the rat. III. Basal and Peg cells. Int J Fertil, 25, 293- 297.
- Culling C F A, Allıson R T, Barr WT(1985) : Cellular Pathology Technique. Fourth edition, Butterworth, Wellington.
- Dziuk P(1991): Reproduction in the pig. 471-487. In: Reproduction in Domestic Animals. Academic press inc.
- Gawronska B, Stepien A, Ziecik AJ (2000): Role of luteinizing hormone in control of oviduct function. Reprod Dom Anim, 35,129-133.
- Hao YH, Yong HY, Murphy CN, Wax D, Samuel M, Rieke A, Lai L, Liu Z, Durtschi DC, Welbern VR, Price EM, McAllister RM, Turk JR, Laughlin MH, Prather RS, Rucker EB (2006): Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets. Transgenic Res, 15,739-750.
- Hunter RHF, Baker TG, Cook B (1982): Morphology, histology and steroid hormones of the gonads in intersex pigs. J Reprod Fert, 64, 217-222.
- Jiwakanon J, Persson E, Kaeoket K, Dalin A-M (2005): The sow endosalpinx at different stages of the oestrous cycle and at anoestrus: Studies on morphological changes and infiltration by cells of the immune system. Reprod Dom Anim, 40, 28-39.
- Johansson M, Tienthai P, Rodriguez-Martinez H (2000): Histochemistry and ultrastructure of the intraluminal mucus in the sperm reservoir of the pig oviduct. J Reprod Dev, 46,183-192.
- Karnovsky M J (1965): A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy, J Cell Biol, 27, 137A-138A.
- Kouba AJ, Abeydeera RL, Alvarez IM, Day BN, Buhi WC (2000): Effects of the porcine oviduct-specific glycoprotein on fertilization, polyspermy and embryologic development in vitro. Biol Reprod, 63,242-250.
- Odor DL (1974): The question of ‘basal’ cells in oviductal and endocervical epithelium. Fertil Steril, 25, 1047- 1062.
- Özen A, Ergün E, Kürüm A (2010): Histomorphology of the oviduct epithelium in the Angora rabbit. Tr J Vet Anim Sci, 34(3), 219-226.
- Paurstein C J, Woodruff J D (1967): The role of the ‘indifferent’ cell of the tubal epithelium. Am J Obstet Gynecol, 98, 121.
- Rodriguez-Martinez H (2007): Role of the oviduct in sperm capacitation. Theriogenology, 68,138-146.
- Shilling E (1974): Stages of ovarian function in the sow. Vet Med Rev,1,59-64.
- Stalheim OHV, Gallagher JE, Deyoe BL (1975): Scanning electron microscopy of the bovine, equine, porcine and caprine uterine tube (oviduct). Am J Vet Res, 36, 1069-1075.
- Steffl M, Schweiger M, Amselgruber WM (2005): Estrous cycle specific immunolocalization of different domains of the epidermal growth factor receptor in the porcine oviduct. Endocrine, 27, 289-294.
- Szulanczyk K (2009): Histological changes within ovarian cortex, oviductal and uterine mucosa in case of ovarian cysts presence in sows. Folia Histochem Cytobiol, 47, 99- 103.
- Tienthai P, Suzuki K, Pertoft H, Kjellen L, Rodriguez- Martinez H (2000): Production of glycosaminoglycans by the porcine oviduct in relation to sperm storage. Reprod Dom Anim, 35, 167-170.
- Tienthai P, Yokoo M, Kimura N, Heldin P,Sato E, Rodriguez-Martinez H (2003): Immunohistochemical localization and expression of the hyaluronan receptor CD44 in the epithelium of the pig oviduct during oestrus. Reproduction, 125,119-132.
- Töpfer-Petersen E (1999): Carbohydrate-based interactions on the route of a spermatozoon to fertilization. Human Reproduction Update, 5, 314-329.
- Veneable J H, Coggeshall R. (1965): A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407- 408.
Year 2013,
Volume: 60 Issue: 1, 7 - 13, 01.03.2013
Abstract
Bu çalışma; domuzlarda ovidukt epitelinin yapısını, ışık ve elektron mikroskobik olarak incelemek, epitel hücreleri tarafından salgılanan mukus içeriğinin histokimyasal yapısını ortaya koymak ve östrus siklusunun farklı dönemlerindeki endotelyal nitrik oksit sentaz (eNOS) aktivitesinin, lokalizasyonu ve dağılımını belirlemek amacıyla yapıldı. Çalışmada 5 adet sağlıklı, erişkin dişi domuzdan alınan ovidukt, materyal olarak kullanıldı. Histokimyasal olarak; östral dönemde artan glikozaminoglikan (GAG) salgısının, özellikle istmusdaki kriptlerde depolandığı görüldü. Elektron mikroskobik olarak; östral dönemde sekretorik hücrelerin sitoplazmalarında artan salgı materyalinin, iki tip salgı granülünde toplandığı belirlendi. İmmunohistokimyasal olarak; hem östral hem de luteal dönemde ampulla ve fimbriyada eNOS ekspresyonu görülmez iken, istmusda ise yalnızca östral dönemde eNOS ekspresyonu saptanmıştır
Keywords
References
- Abe H, Hoshi H (2007): Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig. Reprod Dom Anim, 42, 292-298.
- Abe H, Hoshi H (2008): Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and large white pigs during the oestrous cycle. Reprod Dom Anim, 43,66-73.
- Bancroft J D, Gamble M (2002): Histological Techniques. 5th edition, London, Philadelphia: Churchill & Livingstone, pp.182-189.
- Bullon F, Merchan J A, Gonzalez-Gomez F, Furio V, Poblete EG (1980): Ultrastructure of the oviductal mucosa of the rat. III. Basal and Peg cells. Int J Fertil, 25, 293- 297.
- Culling C F A, Allıson R T, Barr WT(1985) : Cellular Pathology Technique. Fourth edition, Butterworth, Wellington.
- Dziuk P(1991): Reproduction in the pig. 471-487. In: Reproduction in Domestic Animals. Academic press inc.
- Gawronska B, Stepien A, Ziecik AJ (2000): Role of luteinizing hormone in control of oviduct function. Reprod Dom Anim, 35,129-133.
- Hao YH, Yong HY, Murphy CN, Wax D, Samuel M, Rieke A, Lai L, Liu Z, Durtschi DC, Welbern VR, Price EM, McAllister RM, Turk JR, Laughlin MH, Prather RS, Rucker EB (2006): Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets. Transgenic Res, 15,739-750.
- Hunter RHF, Baker TG, Cook B (1982): Morphology, histology and steroid hormones of the gonads in intersex pigs. J Reprod Fert, 64, 217-222.
- Jiwakanon J, Persson E, Kaeoket K, Dalin A-M (2005): The sow endosalpinx at different stages of the oestrous cycle and at anoestrus: Studies on morphological changes and infiltration by cells of the immune system. Reprod Dom Anim, 40, 28-39.
- Johansson M, Tienthai P, Rodriguez-Martinez H (2000): Histochemistry and ultrastructure of the intraluminal mucus in the sperm reservoir of the pig oviduct. J Reprod Dev, 46,183-192.
- Karnovsky M J (1965): A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy, J Cell Biol, 27, 137A-138A.
- Kouba AJ, Abeydeera RL, Alvarez IM, Day BN, Buhi WC (2000): Effects of the porcine oviduct-specific glycoprotein on fertilization, polyspermy and embryologic development in vitro. Biol Reprod, 63,242-250.
- Odor DL (1974): The question of ‘basal’ cells in oviductal and endocervical epithelium. Fertil Steril, 25, 1047- 1062.
- Özen A, Ergün E, Kürüm A (2010): Histomorphology of the oviduct epithelium in the Angora rabbit. Tr J Vet Anim Sci, 34(3), 219-226.
- Paurstein C J, Woodruff J D (1967): The role of the ‘indifferent’ cell of the tubal epithelium. Am J Obstet Gynecol, 98, 121.
- Rodriguez-Martinez H (2007): Role of the oviduct in sperm capacitation. Theriogenology, 68,138-146.
- Shilling E (1974): Stages of ovarian function in the sow. Vet Med Rev,1,59-64.
- Stalheim OHV, Gallagher JE, Deyoe BL (1975): Scanning electron microscopy of the bovine, equine, porcine and caprine uterine tube (oviduct). Am J Vet Res, 36, 1069-1075.
- Steffl M, Schweiger M, Amselgruber WM (2005): Estrous cycle specific immunolocalization of different domains of the epidermal growth factor receptor in the porcine oviduct. Endocrine, 27, 289-294.
- Szulanczyk K (2009): Histological changes within ovarian cortex, oviductal and uterine mucosa in case of ovarian cysts presence in sows. Folia Histochem Cytobiol, 47, 99- 103.
- Tienthai P, Suzuki K, Pertoft H, Kjellen L, Rodriguez- Martinez H (2000): Production of glycosaminoglycans by the porcine oviduct in relation to sperm storage. Reprod Dom Anim, 35, 167-170.
- Tienthai P, Yokoo M, Kimura N, Heldin P,Sato E, Rodriguez-Martinez H (2003): Immunohistochemical localization and expression of the hyaluronan receptor CD44 in the epithelium of the pig oviduct during oestrus. Reproduction, 125,119-132.
- Töpfer-Petersen E (1999): Carbohydrate-based interactions on the route of a spermatozoon to fertilization. Human Reproduction Update, 5, 314-329.
- Veneable J H, Coggeshall R. (1965): A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407- 408.
There are 25 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA86VD23PU |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2013 |
| Published in Issue | Year 2013Volume: 60 Issue: 1 |