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Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope

Year 2012, Volume: 59 Issue: 4, 235 - 239, 01.12.2012

Neslihan Taşçene Hilal Karagül

https://doi.org/10.1501/Vetfak_0000002532

Abstract

The aim of this study is to monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. MDCK cells that were treated with CaCl2, MgSO4, Sodiumcitrate, Na2HPO4 and NaOx/ CaCl2 solutions. MDCK cells that not treated with any chemicals are used as control group. Calcium islets were observed in cells that treated with CaCl2 and calcium oxalate crystals which binded to the cells were observed in cells that treated with NaOx/CaCl2 solution. The MDCK cells that treated with Na-citrate solution, there was any difference was found when compared with control cells. The MDCK cells, treated with Na2HPO4 solution, necrotic areas were found

Keywords

MDCK cell line CaCl2 Na-sitrat MgSO4 NaHPO4 Calcium oxalate

References

  • Aiello SE (1998): The Merck Veterinary Manual. 8th Ed. Merck Co. Inc. Philadelphia.
  • Coe F, Evan A, Worcester E (2005): Kidney Stone Disease. J Clin Invest. 115, 2598–2608.
  • Hazıroğlu R., Milli Ü (2001): Veteriner Patoloji Cilt I. Medipres Matbaacılık-Yayıncılık.
  • Herring Lc (1962): Observations On The Analysis Of Ten Thousand Urinary Calculi. J Urol. 88, 545–562.
  • Jonassen JA, Kohjimoto,Y, Scheid, CR, Schmidt M (2005): Oxalate toxicity in renal cells. Urol.Res. 33, 329- 339.
  • Karagül H, Altıntaş A, Fidanci UR, Sel T (2000): Klinik Biyokimya. Medisan Yayınevi. 1. Baskı. Yayın Serisi:45. Ankara.
  • Khan SR (2004): Crystal-İnduced İnflammation of The Kidneys: Results From Human Studies,Animal Models, and Tissue-Culture Studies. Clin Exp Nephrol. 8,75–88.
  • Khan SR,. Canales BK (2009): Genetic Basis Of Renal Cellular Dysfunction and The Formation of Kidney Stones. Urol Res. 37,169–180.
  • Lieske J C, Toback FG (2000): Renal Cell-Urinary Crystal İnteractions. Curr. Opin. Nephrol. Hypertens. 9, 349–355.
  • Mazzali, M., Kipari T, Ophascharoensk V, Wesson JA, Johnson R, Hughes J (2002) : Osteopontin- A Molecule For All Seasons. Q J Med., 95, 3-13.
  • Okada A, Yasui T, Fujii Y, Niimi K, Hamamoto S, Hirose M, Kojima Y, Itoh Y, Tozawa K, Hayashi Y, Kohri K. (2010): Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation. J Bone Miner Res. 25:2701-11
  • Osborne CA., Lulich JP, Polzin DJ (1999): Analysis of 77000 canine uroliths. Vet. Clin. North Am. Small Anim. Pract. 29,17-38.
  • Ouyang JM, Yao XQ, Tan J, Wang FX (2011): Renal Epithelial Formation Of Calcium Oxalate Monohydrate. J Biol Inorg Chem. 16, 405-416.
  • Prien El, Prien El Jr (1968): Composition And Structure Of Urinary Stone. Am J Med 45,654–672.
  • Tilley LP, Smith FMK (1997): The 5 Minute Veterinary Consult Canine and Feline. Williams & Wilkins Campany. Pennsylvania, U.S.A.
  • Verkoelen CF, Schepers MSJ, Ballegooijen ES, Bangma CH (2005): Effects of luminal oxalate or calcium oxalate on renal tubular cells in culture. Urol.Res. 33, 321-328.
  • Yuen JW, Gohel MD, Poon NW, Shum DK, Tam PC, Au DW.(2010): The initial and subsequent inflammatory events during calcium oxalate lithiasis. Clin Chim Acta. 411: 1018-1026.

Farklı kültür ortamlarının MDCK hücrelerine etkilerinin faz kontrast mikroskop ile gösterilmesi

Year 2012, Volume: 59 Issue: 4, 235 - 239, 01.12.2012

Neslihan Taşçene Hilal Karagül

https://doi.org/10.1501/Vetfak_0000002532

Abstract

Deneysel olarak Kalsiyumklorür (CaCl2), Magnezyumsülfat (MgSO4), Sodyumsitrat, Sodyumhidrojenfosfat (Na2HPO4) ve Kalsiyum okzalat solusyonlarına maruz bırakılan böbrek hücrelerindeki etkiler faz kontrast mikroskopla görüntülenmiştir. Hiçbir kimyasalla muamele edilmeyen Madin Darby Canine Kidney (MDCK) hücreleri kontrol grubu olarak kullanılmıştır. CaCl2 eklenmis grubun faz kontrast mikroskobik görüntüsünde yer yer kalsiyum birikintilerinin, kalsiyum okzalat eklenen grupta ise hücrelere tutunmus halde kalsiyum okzalat kristallerinin olustuğu gözlenmistir. MgSOçözeltisi eklenen MDCK hücrelerinde yer yer dejenere bölgelere rastlanmıştır. Na-sitrat çözeltisi eklenen MDCK hücreleri, kontrol hücreleriyle karşılaştırıldığı zaman büyük bir farklılığa rastlanmamıştır. Na2HPO4 çözeltisi eklenen MDCK hücrelerinde ise hücreler arasında yer yer nekrotik alanlara rastlanmıştır

Keywords

MDCK hücre hattı CaCl2 Na-sitrat MgSO4 Na2HPO4 Kalsiyum okzalat

References

  • Aiello SE (1998): The Merck Veterinary Manual. 8th Ed. Merck Co. Inc. Philadelphia.
  • Coe F, Evan A, Worcester E (2005): Kidney Stone Disease. J Clin Invest. 115, 2598–2608.
  • Hazıroğlu R., Milli Ü (2001): Veteriner Patoloji Cilt I. Medipres Matbaacılık-Yayıncılık.
  • Herring Lc (1962): Observations On The Analysis Of Ten Thousand Urinary Calculi. J Urol. 88, 545–562.
  • Jonassen JA, Kohjimoto,Y, Scheid, CR, Schmidt M (2005): Oxalate toxicity in renal cells. Urol.Res. 33, 329- 339.
  • Karagül H, Altıntaş A, Fidanci UR, Sel T (2000): Klinik Biyokimya. Medisan Yayınevi. 1. Baskı. Yayın Serisi:45. Ankara.
  • Khan SR (2004): Crystal-İnduced İnflammation of The Kidneys: Results From Human Studies,Animal Models, and Tissue-Culture Studies. Clin Exp Nephrol. 8,75–88.
  • Khan SR,. Canales BK (2009): Genetic Basis Of Renal Cellular Dysfunction and The Formation of Kidney Stones. Urol Res. 37,169–180.
  • Lieske J C, Toback FG (2000): Renal Cell-Urinary Crystal İnteractions. Curr. Opin. Nephrol. Hypertens. 9, 349–355.
  • Mazzali, M., Kipari T, Ophascharoensk V, Wesson JA, Johnson R, Hughes J (2002) : Osteopontin- A Molecule For All Seasons. Q J Med., 95, 3-13.
  • Okada A, Yasui T, Fujii Y, Niimi K, Hamamoto S, Hirose M, Kojima Y, Itoh Y, Tozawa K, Hayashi Y, Kohri K. (2010): Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation. J Bone Miner Res. 25:2701-11
  • Osborne CA., Lulich JP, Polzin DJ (1999): Analysis of 77000 canine uroliths. Vet. Clin. North Am. Small Anim. Pract. 29,17-38.
  • Ouyang JM, Yao XQ, Tan J, Wang FX (2011): Renal Epithelial Formation Of Calcium Oxalate Monohydrate. J Biol Inorg Chem. 16, 405-416.
  • Prien El, Prien El Jr (1968): Composition And Structure Of Urinary Stone. Am J Med 45,654–672.
  • Tilley LP, Smith FMK (1997): The 5 Minute Veterinary Consult Canine and Feline. Williams & Wilkins Campany. Pennsylvania, U.S.A.
  • Verkoelen CF, Schepers MSJ, Ballegooijen ES, Bangma CH (2005): Effects of luminal oxalate or calcium oxalate on renal tubular cells in culture. Urol.Res. 33, 321-328.
  • Yuen JW, Gohel MD, Poon NW, Shum DK, Tam PC, Au DW.(2010): The initial and subsequent inflammatory events during calcium oxalate lithiasis. Clin Chim Acta. 411: 1018-1026.
There are 17 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA97KR99JS
Journal Section Research Article
Authors

Neslihan Taşçene

Hilal Karagül

Publication Date December 1, 2012
Published in Issue Year 2012Volume: 59 Issue: 4

Cite

APA Taşçene, N., & Karagül, H. (2012). Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 59(4), 235-239. https://doi.org/10.1501/Vetfak_0000002532
AMA Taşçene N, Karagül H. Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. Ankara Univ Vet Fak Derg. December 2012;59(4):235-239. doi:10.1501/Vetfak_0000002532
Chicago Taşçene, Neslihan, and Hilal Karagül. “Monitorize Effects of Chemicals That Used in the Study on MDCK Cells With Phase Contrast Microscope”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59, no. 4 (December 2012): 235-39. https://doi.org/10.1501/Vetfak_0000002532.
EndNote Taşçene N, Karagül H (December 1, 2012) Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 4 235–239.
IEEE N. Taşçene and H. Karagül, “Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope”, Ankara Univ Vet Fak Derg, vol. 59, no. 4, pp. 235–239, 2012, doi: 10.1501/Vetfak_0000002532.
ISNAD Taşçene, Neslihan - Karagül, Hilal. “Monitorize Effects of Chemicals That Used in the Study on MDCK Cells With Phase Contrast Microscope”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59/4 (December 2012), 235-239. https://doi.org/10.1501/Vetfak_0000002532.
JAMA Taşçene N, Karagül H. Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. Ankara Univ Vet Fak Derg. 2012;59:235–239.
MLA Taşçene, Neslihan and Hilal Karagül. “Monitorize Effects of Chemicals That Used in the Study on MDCK Cells With Phase Contrast Microscope”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 59, no. 4, 2012, pp. 235-9, doi:10.1501/Vetfak_0000002532.
Vancouver Taşçene N, Karagül H. Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. Ankara Univ Vet Fak Derg. 2012;59(4):235-9.