Chemical composition and antibacterial activity of essential oils from different parts of endemic Bupleurum L. species
Abstract
The essential oils of Bupleurum heldreichii Boiss. & Bal., Bupleurum sulphureum Boiss. & Bal., Bupleurum turcicum Snogerup, and Bupleurum lycaonicum Snogerup flowers, fruits and roots were obtained using hydrodistillation and microdistillation techniques and their chemical compositions were analyzed by GC and GC/MS systems, simultaneously. The antibacterial activity of the oils which obtained by hydrodistillation was assessed with micro-dilution assays. The main components of B. heldreichii were germacrene D (% 47.5-48.4) in flowers and fruits, and hexadecanoic acid (% 46.2) in roots. The main components of B. sulphureum found undecane (% 14.0-20.2) in flowers and fruits, and calarene (% 26.9) in roots. The main components of B. turcicum were heptanal (% 33.2-23.5) in flowers and fruits, and pentacosane (% 9.0) in roots. The main components of B. lycaonicum were tridecane (% 14.9-37.3) in flowers and roots, spathulenol (% 14.4) in fruits. The essential oils of B. heldreichii, B. sulphureum, B. turcicum obtained from flowers and fruits, B. lycaonicum from fruits used in the study did not have any effect against bacteria. The MIC values of essential oils of the roots for the bacterial strains tested, which were sensitive to the essential oils of roots of B. heldreichii, B. sulphureum and B. turcicum were in the ratio of 2 mg/ml. This investigation showed that the antibacterial activity of B. heldreichii, B. sulphureum and B. turcicum was attributed to the essential oil of roots, thus they can be a potential medicinal resource
References
- Akın M, Demirci B, Bağcı Y, Başer KHC (2010): Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey. African J Biotechnol, 9(15), 2323.
- Bermejo Benito P, Abad Martinez MJ, Silvan Sen SM, Sanz Gomez A, Fernandez Matellano L, Sanchez Contreras S, Diaz Lanza AM (1998): In vivo and in vitro anti-inflammatory activity of saikosaponins. Life Sci, 63, 1147-1156.
- Davis PH (1982): Flora of Turkey and the East Aegean Islands. Vol. 4, University Press, Edinburgh, 393-418.
- Dorman HJD, Deans SG (2000): Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol, 88, 308.
- Griffin SG, Wyllie SG, Markham JL, Leach DN (1999): The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Fragrance J, 14, 322.
- Güner A, Özhatay N, Ekim T, Başer KHC (2000): Flora of Turkey and the East Aegean Islands. Vol. 11, University Press, Edinburgh, 143-144.
- Hammer KA, Carson JF, Riley TV (1999): Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol, 86, 985-990.
- Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC (1997): Color Atlas and Textbook of Diagnostic Microbiology. Lippincott-Raven Publ, Philadelphia, 785-856.
- Motoo Y, Sawabu N (1994): Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines. Cancer Lett, 86, 91-95.
- Nose M, Amagaya S, Ogihara Y (1989): Corticosterone secretion-inducing activity of saikosaponin metabolites formed in the alimentary tract. Chem Pharm Bull, 37, 2736-40.
- Pan SL (2006): Bupleurum Species Scientific Evaluation and Clinical Applications. Taylor & Francis Group Boca Raton, London, New York, 1.
- Van Wyk BE, Wink M (2004): Medicinal Plants of the World: an Illustrated Scientific Guide to Important Medicinal Plants and Their Uses. 1st ed. Portland, Orlando, Timber Press.
- Wu JN (2005): An Illustrated Chinese Materia Medica. New York, Oxford University Press.
- Zgoda JR, Porter JR (2001): A convenient microdilution method for screening natural products against bacteria and fungi. Pharm Microbiol, 39, 221-225.
Endemik Bupleurum L. türlerinin farklı kısımlarının uçucu yağlarının kimyasal kompozisyonu veantibakteriyel aktivitesi
Abstract
Bupleurum heldreichii Boiss. & Bal., Bupleurum sulphureum Boiss. & Bal., Bupleurum turcicum Snogerup ve Bupleurum lycaonicum Snogerup’un çiçek, meyve ve köklerinin uçucu yağları hidrodistilasyon ve mikrodistilasyon yöntemleriyle elde edildi ve kimyasal bileşimleri GC ve GC/MS sistemleri ile eşzamanlı olarak incelendi. Hidrodistilasyon ile elde edilen uçucu yağların antibakteriyel aktiviteleri mikrodilüsyon yöntemiyle belirlendi. B. heldreichii’nin çiçek ve meyvelerinde germakren D (% 47.5-48.4), köklerinde hekzadekanoik asit (% 46.2) ana bileşenler olarak belirlendi. Ana bileşenler B. sulphureum’un çiçeklerinde ve meyvelerinde undekan (% 14.0-20.2), köklerinde kalaren (% 26.9) olarak bulundu. B. turcicum’un çiçeklerinde ve meyvelerinde heptanal (% 33.2-23.5), köklerinde pentakosan (% 9.0) ana bileşenler olarak belirlendi. B. lycaonicum’un çiçeklerinde ve köklerinde tridekan (% 14.9-37.3), meyvelerinde spatulenol (% 14.4) ana bileşenler olarak bulundu. B. heldreichii, B. sulphureum, B. turcicum’un çiçek ve meyvelerinden, B. lycaonicum’un meyvelerinden elde edilen uçucu yağların çalışmada kullanılan bakterilere karşı etkisinin olmadığı görüldü. B. heldreichii, B. sulphureum ve B. turcicum’un kök uçucu yağlarının test edilen bakteri suşları için MİK değerleri 2 mg/ml’dir. Bu araştırma B. heldreichii, B. sulphureum ve B. turcicum’un kök uçucu yağlarının antibakteriyel aktivitesinin olduğunu ve potansiyel tıbbi kaynak olabileceğini gösterdi
References
- Akın M, Demirci B, Bağcı Y, Başer KHC (2010): Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey. African J Biotechnol, 9(15), 2323.
- Bermejo Benito P, Abad Martinez MJ, Silvan Sen SM, Sanz Gomez A, Fernandez Matellano L, Sanchez Contreras S, Diaz Lanza AM (1998): In vivo and in vitro anti-inflammatory activity of saikosaponins. Life Sci, 63, 1147-1156.
- Davis PH (1982): Flora of Turkey and the East Aegean Islands. Vol. 4, University Press, Edinburgh, 393-418.
- Dorman HJD, Deans SG (2000): Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol, 88, 308.
- Griffin SG, Wyllie SG, Markham JL, Leach DN (1999): The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Fragrance J, 14, 322.
- Güner A, Özhatay N, Ekim T, Başer KHC (2000): Flora of Turkey and the East Aegean Islands. Vol. 11, University Press, Edinburgh, 143-144.
- Hammer KA, Carson JF, Riley TV (1999): Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol, 86, 985-990.
- Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC (1997): Color Atlas and Textbook of Diagnostic Microbiology. Lippincott-Raven Publ, Philadelphia, 785-856.
- Motoo Y, Sawabu N (1994): Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines. Cancer Lett, 86, 91-95.
- Nose M, Amagaya S, Ogihara Y (1989): Corticosterone secretion-inducing activity of saikosaponin metabolites formed in the alimentary tract. Chem Pharm Bull, 37, 2736-40.
- Pan SL (2006): Bupleurum Species Scientific Evaluation and Clinical Applications. Taylor & Francis Group Boca Raton, London, New York, 1.
- Van Wyk BE, Wink M (2004): Medicinal Plants of the World: an Illustrated Scientific Guide to Important Medicinal Plants and Their Uses. 1st ed. Portland, Orlando, Timber Press.
- Wu JN (2005): An Illustrated Chinese Materia Medica. New York, Oxford University Press.
- Zgoda JR, Porter JR (2001): A convenient microdilution method for screening natural products against bacteria and fungi. Pharm Microbiol, 39, 221-225.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA47UF47EM |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2012 |
| Published in Issue | Year 2012Volume: 59 Issue: 4 |