Effects of ascorbic and α-lipoic acid on secretion of HSP- 70 and apoptosis in liver and kidneys of broilers exposed to heat stress
Abstract
The objective of this experiment was to evaluate the ameliorative effect of L-ascorbic acid (AA) and α-lipoic acid (ALA) on tissue damage caused by heat stress (HS) in the liver and kidneys of broilers, and to correlate this effect with the secretion of the heat shock protein-70 (HSP-70) protein and apoptosis. The study broilers of breed Ross 308 were used. Animals included in the control were divided into four groups. Group TN; control thermoneutral broilers (24 0C) were not administered with antioxidants in the diet and not subjected to heat stress (TN). Group HS; broilers not administered with antioxidants in the diet but subjected to heat stress (34 0C) (HS). Group HS+AA; broilers subjected to heat stress (34 0C) and administered with ascorbic acide (AA). HS+ALA; broilers subjected to heat stress (34 0C) and administered with α- lipoic acid (AAA). Then, all of the groups were sacrificed and organs (liver and kidney) were collected for histopathological and immunohistochemstry evaluations. Histopathological examination demonstrated that, in the liver and kidneys in Group HS, the blood vessels were very hyperemic. Hepatocytes as well as in tubulo epithelial cells of kidney displayed degenerative alterations. Lesions similar to those observed in Group HS were also observed in Groups HS+AA and HS+ALA. However, the liver and kidney HSP-70 secration were greater in Groups HS+AA, HS+ALA and TN than in Groups HS. The greatest number of apoptotic cells was determined in Group HS, whilst the lowest numbers were determined in Groups TN and HS+ALA. Dietary supplementation with ALA was more effective than AA at preventing tissue damage
References
- Alexandrov VY (1994): Function: aspects of cell response to heat shock. Int Rev Cytol, 148,171–227.
- Bao ED, Gong YY, Hartung J, Hartung J, Fu XB, Wang XJ, Zhang HT, Wang ZL (2004): Relation between pathologic damages and HSP70 changes in acute heat stressed broilers. Scientia Agricultura Sinica, 37, 301- 305.
- Cohen JJ, Duke RC, Fadok VA, Sellins KS (1992): Apoptosis and programmed cell death in immunity. Annu Rev Immunol, 10, 267–293.
- Ensminger ME, Oldfield JE, Heinemann WW (1990): Feeding.108–110. In W.W. Heinemann (Ed). Feeds and Nutrition California: Ensminger Publishing.
- Gonzalez-Perez O, Gonzalez-Castaneda R (2006): Therapeutic perspectives on the combination of a-lipoic acid and vitamin E. Nutr Res, 26, 1– 5.
- Gupte AA, Bomhoff GL, Morris JK, Gorres BK, Geiger PC (2009): Lipoic acid increases heat shock protein expression and inhibits stress kinase activation to improve insulin signaling in skeletal muscle from high-fat- fed rats. J Appl Physiol, 106,1425–1434.
- Guler G, Ozgur E, Keleş H, Tomtuk A, Atalay Vural S, Seyhan N (2011): Apoptosis resulted from radiofrequency radiation exposure of pregnant rabbits and their infants. Bull Vet Inst Pulawy, 55, 127-134.
- Kafri I, Cherry JA (1984): Supplemental ascorbic acid and heat stress in broiler chicks. Poultry Sci, 63, 125–126.
- Kim JE, Kang JS, Jung DJ, Hahm E, Lee SK, Bae SY, Hwang YI, Shin DH, Lee WJ (2007): Vitamin C induces apoptosis in human colon cancer cells through endoplasmic reticulum stress and mitochondrial pathway. J Immunol, 178, 49-25.
- Kinnunen S, Hyyppä S, Oksala N, Laaksonen DE, Hannila ML, Sen CK, Atalay M (2009): α-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standard bred trotters. Res Vet Sci, 87, 462– 467.
- Koc M, Imik H, Odabasoglu F (2008): Gastroprotective and anti-oxidative properties of ascorbic acid on indomethacin-induced gastric injuries in rats. Biol Trace Elem Res, 126, 222–236.
- Lanneau D, Brunet M, Frisan E, Solary E, Fontenay M, Garrido C (2008): Heat shock proteins: essential proteins for apoptosis regulation. J Cell Mol Med, 12, 743-61.
- Lin H, Decuypere E, Buyse J (2006): Acute heat stress induce oxidative stress in broiler chickens. Comp Biochem Physt A, 144, 11–17.
- Mahmoud KZ, Edens FW, Eisen EJ, Havenstein GB (2003): Effect of ascorbic acid and acute heat exposure on heat shock protein 70 expression by young White Leghorn chickens. Comp Biochem Phys C, 136, 329–335.
- Mahmoud KZ, Edens FW, Eisen EJ, Havenstein GB (2004): Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers (Gallus gallus domesticus) subjected to cyclic heat stress. Comp Biochem Physiol B Biochem Mol Biol. 137, 35–42.
- Mahmoud KZ, Edens FW (2005): Influence of organic selenium on HSP70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp Biochem Physiol C Toxicol Pharmacol, 141, 69 -75.
- Moungjaroen J, Nimmannit U, Callery PS, Wang L, Azad N, Lipipun V, Chanvorachote P, Rojanasakul Y (2006): Reactive Oxygen Species Mediate Caspase Activation and Apoptosis Induced by Lipoic Acid in Human Lung Epithelial Cancer Cells through Bcl-2 Down- regulation. J Pharmacol Exp Ther, 319, 1062–1069.
- Multhoff G (2006): Heat shock proteins in immunity. Handb Exp Pharmacol, 172, 279–304.
- National Research Council (1994): Nutrient Requirements of Poultry. Edited by the National Academy Press, Washington, DC.
- Ojeda F; Skardova I; Guarda MI; Ulloa J; Folch H (1997). Proliferation and apoptosis in infection with infectious bursal disease virus: a flow cytometric study. Avian Dis, 41:312-316
- Pei-Ming S, Yu-Tian L, Qing-Hua W, Zhi-Liang W, En-Dong B (2007): HSP70 and HSP70 mRNA localization in heat-stressed tissues of broilers. Chinese J Agr Biotechol, 4, 181–186.
- Pirlich M, Kiok K, Sandig G, Lochs H, Grune T (2002): Alpha-lipoic acid prevents ethanol-induced protein oxidation in mouse hippocampal HT22 cell. Neurosci Lett, 328, 93–96.
- Sharma HS, Muresanu D, Sharma A, Zimmermann- Meinzingen S (2010): Cerebrolysin treatment attenuates heat shock protein overexpression in the brain following heat stress: an experimental a study using immunohistochemistry at light and electron microscopy in the rat. Ann NY Acad Sci, 1199, 138-48.
- Sahin N, Tuzcu M, Orhan C, Onderci M, Eroksuz Y, Sahin K (2009): The effects of vitamin C and E supplementation on heat shock protein 70 response of ovary and brain in heat-stressed quail. Brit Poultry Sci, 50, 259-265.
- See AP, Pradilla G, Yang I, Han S, Parsa, AT, Lim M (2011): Heat shock protein-peptide coplex in the treatment of glioblastoma. Expert Rev Vaccines, 10, 721-731.
- Seyrek K, Yenisey C, Serter M, Kiral FK, Ulutas PA, Bardakcioglu HE (2004): Effects of Dietary Vitamin C Supplementation on Some Serum Biochemical Parameters of Laying Japanese Quails Exposed To Heat Stress (34.8°C). Rev Méd Vét, 155, 339-342.
- Sherman M, Multhoff G (2007): Heat shock proteins in cancer. Ann NY Acad Sci, 1113, 192–201.
- Siegel HS (1995): Stress, strains and resistance. Brit Poultry Sci, 36, 3–22.
- SPSS (1996): Statistical Packages for the Social Sciences for Windows release 10.01. SPSS Inc., Chicago.
- Van Eden W, van Der Zee R, Prakken B (2005): Heat shock proteins induce T cell regulation of chronic inflammation. Nat Rev Immunol, 5, 318–330
- Wang S, Edens FW (1998): Heat conditioning induces heat shock proteins in broiler chickens and turkey poults. Poultry Sci, 77, 1636 –1645
Sıcaklık stresine maruz bırakılan broilerlerin karaciğer ve böbreklerinde α-lipoik ve askorbik asitinHSP- 70 sekresyonu ve apoptosis üzerine etkileri
Abstract
Bu çalışmada sıcaklık stresinin broilerlerin böbrek ve karaciğerlerinde meydana getireceği doku hasarı üzerine Laskorbik (AA) ve α- lipoik (ALA) asit’in koruyucu etkinin varlığı ve bu etkide HSP-70 salınımı ile apoptozis arasındaki ilişkinin saptanması amaçlanmıştır. Çalışmada toplam 278 Ross 308 ırkı broiler kullanıldı. Hayvanlar kontrol grubuda dahil 4 gruba ayrıldı. Grup TN; sıcaklık stresine maruz bırakılmayan (24 0C) ve diyetlerine antioksidan eklenmeyen termonötral kontrol broiler (TN). Grup HS; sıcaklık stresine maruz bırakılan (34 0C) ancak diyetlerine antioksidan eklenen broiler (HS). Grup HS+AA; sıcaklık stresine maruz bırakılan (34 0C) ancak diyetlerine askorbik asit (AA) eklenen broiler. Grup HS+AAA; sıcaklık stresine maruz bırakılan (34 0C) ancak diyetlerine α- lipoik asit (ALA) eklenen broiler. Sonra, grupların tamamı öldürüldü ve organlar (karaciğer ve böbrek) histopatolojik ve immunohistokimyasal değerlendirmeler için toplandı. Histopatolojik muayenelerde HS grubunun karaciğer ve böbreklerinde damarların oldukça hiperemik olduğu gözlendi. Hepatositlerde olduğu kadar böbrek epitel hücrelerinde de dejeneratif değişikler gözlendi. HS grubunda gözlenen lezyonların benzeri HS+AA, HS+ALA gruplarında da gözlendi. Ancak böbrek ve karaciğerde HSP-70 sekresyonu HS ve TN gruplarıyla kıyaslandığında HS+AA and HS+ALA gruplarında daha fazlaydı. Apoptotik hücre sayısı en fazla HS grubunda gözlenirken, en düşük hücre sayısı TN ve HS+ALA gruplarında gözlendi. Diyete eklenen ALA’nın doku hasarını önlemede AA’ya göre çok daha etkili olduğu gözlendi
References
- Alexandrov VY (1994): Function: aspects of cell response to heat shock. Int Rev Cytol, 148,171–227.
- Bao ED, Gong YY, Hartung J, Hartung J, Fu XB, Wang XJ, Zhang HT, Wang ZL (2004): Relation between pathologic damages and HSP70 changes in acute heat stressed broilers. Scientia Agricultura Sinica, 37, 301- 305.
- Cohen JJ, Duke RC, Fadok VA, Sellins KS (1992): Apoptosis and programmed cell death in immunity. Annu Rev Immunol, 10, 267–293.
- Ensminger ME, Oldfield JE, Heinemann WW (1990): Feeding.108–110. In W.W. Heinemann (Ed). Feeds and Nutrition California: Ensminger Publishing.
- Gonzalez-Perez O, Gonzalez-Castaneda R (2006): Therapeutic perspectives on the combination of a-lipoic acid and vitamin E. Nutr Res, 26, 1– 5.
- Gupte AA, Bomhoff GL, Morris JK, Gorres BK, Geiger PC (2009): Lipoic acid increases heat shock protein expression and inhibits stress kinase activation to improve insulin signaling in skeletal muscle from high-fat- fed rats. J Appl Physiol, 106,1425–1434.
- Guler G, Ozgur E, Keleş H, Tomtuk A, Atalay Vural S, Seyhan N (2011): Apoptosis resulted from radiofrequency radiation exposure of pregnant rabbits and their infants. Bull Vet Inst Pulawy, 55, 127-134.
- Kafri I, Cherry JA (1984): Supplemental ascorbic acid and heat stress in broiler chicks. Poultry Sci, 63, 125–126.
- Kim JE, Kang JS, Jung DJ, Hahm E, Lee SK, Bae SY, Hwang YI, Shin DH, Lee WJ (2007): Vitamin C induces apoptosis in human colon cancer cells through endoplasmic reticulum stress and mitochondrial pathway. J Immunol, 178, 49-25.
- Kinnunen S, Hyyppä S, Oksala N, Laaksonen DE, Hannila ML, Sen CK, Atalay M (2009): α-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standard bred trotters. Res Vet Sci, 87, 462– 467.
- Koc M, Imik H, Odabasoglu F (2008): Gastroprotective and anti-oxidative properties of ascorbic acid on indomethacin-induced gastric injuries in rats. Biol Trace Elem Res, 126, 222–236.
- Lanneau D, Brunet M, Frisan E, Solary E, Fontenay M, Garrido C (2008): Heat shock proteins: essential proteins for apoptosis regulation. J Cell Mol Med, 12, 743-61.
- Lin H, Decuypere E, Buyse J (2006): Acute heat stress induce oxidative stress in broiler chickens. Comp Biochem Physt A, 144, 11–17.
- Mahmoud KZ, Edens FW, Eisen EJ, Havenstein GB (2003): Effect of ascorbic acid and acute heat exposure on heat shock protein 70 expression by young White Leghorn chickens. Comp Biochem Phys C, 136, 329–335.
- Mahmoud KZ, Edens FW, Eisen EJ, Havenstein GB (2004): Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers (Gallus gallus domesticus) subjected to cyclic heat stress. Comp Biochem Physiol B Biochem Mol Biol. 137, 35–42.
- Mahmoud KZ, Edens FW (2005): Influence of organic selenium on HSP70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp Biochem Physiol C Toxicol Pharmacol, 141, 69 -75.
- Moungjaroen J, Nimmannit U, Callery PS, Wang L, Azad N, Lipipun V, Chanvorachote P, Rojanasakul Y (2006): Reactive Oxygen Species Mediate Caspase Activation and Apoptosis Induced by Lipoic Acid in Human Lung Epithelial Cancer Cells through Bcl-2 Down- regulation. J Pharmacol Exp Ther, 319, 1062–1069.
- Multhoff G (2006): Heat shock proteins in immunity. Handb Exp Pharmacol, 172, 279–304.
- National Research Council (1994): Nutrient Requirements of Poultry. Edited by the National Academy Press, Washington, DC.
- Ojeda F; Skardova I; Guarda MI; Ulloa J; Folch H (1997). Proliferation and apoptosis in infection with infectious bursal disease virus: a flow cytometric study. Avian Dis, 41:312-316
- Pei-Ming S, Yu-Tian L, Qing-Hua W, Zhi-Liang W, En-Dong B (2007): HSP70 and HSP70 mRNA localization in heat-stressed tissues of broilers. Chinese J Agr Biotechol, 4, 181–186.
- Pirlich M, Kiok K, Sandig G, Lochs H, Grune T (2002): Alpha-lipoic acid prevents ethanol-induced protein oxidation in mouse hippocampal HT22 cell. Neurosci Lett, 328, 93–96.
- Sharma HS, Muresanu D, Sharma A, Zimmermann- Meinzingen S (2010): Cerebrolysin treatment attenuates heat shock protein overexpression in the brain following heat stress: an experimental a study using immunohistochemistry at light and electron microscopy in the rat. Ann NY Acad Sci, 1199, 138-48.
- Sahin N, Tuzcu M, Orhan C, Onderci M, Eroksuz Y, Sahin K (2009): The effects of vitamin C and E supplementation on heat shock protein 70 response of ovary and brain in heat-stressed quail. Brit Poultry Sci, 50, 259-265.
- See AP, Pradilla G, Yang I, Han S, Parsa, AT, Lim M (2011): Heat shock protein-peptide coplex in the treatment of glioblastoma. Expert Rev Vaccines, 10, 721-731.
- Seyrek K, Yenisey C, Serter M, Kiral FK, Ulutas PA, Bardakcioglu HE (2004): Effects of Dietary Vitamin C Supplementation on Some Serum Biochemical Parameters of Laying Japanese Quails Exposed To Heat Stress (34.8°C). Rev Méd Vét, 155, 339-342.
- Sherman M, Multhoff G (2007): Heat shock proteins in cancer. Ann NY Acad Sci, 1113, 192–201.
- Siegel HS (1995): Stress, strains and resistance. Brit Poultry Sci, 36, 3–22.
- SPSS (1996): Statistical Packages for the Social Sciences for Windows release 10.01. SPSS Inc., Chicago.
- Van Eden W, van Der Zee R, Prakken B (2005): Heat shock proteins induce T cell regulation of chronic inflammation. Nat Rev Immunol, 5, 318–330
- Wang S, Edens FW (1998): Heat conditioning induces heat shock proteins in broiler chickens and turkey poults. Poultry Sci, 77, 1636 –1645
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA99TN46EH |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2012 |
| Published in Issue | Year 2012Volume: 59 Issue: 4 |