Abstract
Recently, it has been discovered that the doses of the MK-801 causing schizophrenia also initiate the oxidative stress in the testis. The current study investigated the protective role of the resveratrol against the MK-801 induced oxidative stress in the testis in mice. The testis weight, the total oxidant-antioxidant status, seminiferous tubules diameter, epithelial height, testicular pathology, and epididymal sperm motility were evaluated. A total of 24 male mice were equally divided into 4 groups so that each group included 6 mice. In the study, Group I (control group) was intraperitoneally received with 0.9% saline (10ml/kg). Group II was i.p. administered with MK-801 (1mg/kg), Group III was treated with i.p. MK-801 (1mg/kg) and resveratrol (40mg/kg), and the Group IV was treated i.p. with resveratrol (40mg/kg). All injections were performed for 14 days. According to the results, in the testis of mice in Group II the oxidative stress was observed. The oxidative stress affected the seminiferous tubules pathologically and decreased the weight of the testis and sperm motility. However, resveratrol protected the MK-801 induced oxidative stress in the testis. Moreover, this dose of the resveratrol increased the sperm motility compared with the controls. In conclusion, MK-801 caused oxidative stress in the testis and resveratrol had a protective effect against this damage.
Keywords
References
- 1. Araghi A, Tabari AM, Golshahi H (2017): Protective effect of carvacrol on ketamine induced testicular damage in mouse model of schizophrenia. Journal of HerbMed Pharmacology, 6(3), 100-106.
- 2. Cui X, Jing X, Wu X, et al. (2016): Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity. Molecular Medicine Reports, 14(5), 4659-4665.
- 3. Erel O (2004): A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(2), 112-119.
- 4. Erel O (2005): A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-1111.
- 5. Faid I, Al-Hussaini H, Kilarkaje N (2015): Resveratrol alleviates diabetes-induced testicular dysfunction by inhibiting oxidative stress and c-Jun N-terminal kinase signaling in rats. Toxicology and Applied Pharmacology, 289(3), 482-94.
- 6. Hu JH, Yang N, Ma YH, et al. (2004): Identification of glutamate transporters and receptors in mouse testis. Acta Pharmacologica Sinica, 25(3), 366-371.
- 7. Kovacic P, Somanathan R (2010): Clinical physiology and mechanism of dizocilpine (MK-801) Electron transfer, radicals, redox metabolites and bioactivity. Oxidative Medicine and Cellular Longevity, 3(1), 13-22.
- 8. Kruk-Slomka M, Budzynska B, Slomka T, et al. (2016): The influence of the CB1 receptor ligands on the schizophrenia-like effects in mice induced by MK-801. Neurotoxicity Research, 30(4), 658-676.
- 9. Lewis DA, Lieberman JA (2000): Catching up on Schizophrenia: Natural history and neurobiology. Neuron, 28(2), 325-34.
- 10. Mendes TB, Paccola CC, de Oliveira Neves FM, et al. (2016): Resveratrol improves reproductive parameters of adult rats varicocelized in peripuberty. Reproduction, 152(1), 23-35.
- 11. Ourique GM, Finamor IA, Saccol EM, et al. (2013): Resveratrol improves sperm motility, prevents lipid peroxidation and enhances antioxidant defences in the testes of hyperthyroid rats. Reproductive Toxicology, 37, 31-39.
- 12. Ozyurt B, Parlaktas BS, Ozyurt H, et al. (2007): A preliminary study of the levels of testis oxidative stress parameters after MK-801-induced experimental psychosis model: protective effects of CAPE. Toxicology, 230, 83-89.
- 13. Pankevich DE, Davis M, Altevogt BM (2011): Glutamate-Related biomarkers in drug development for disorders of the nervous system: Workshop summary. National Academies Press (US), Washington (DC). pp. 5.
- 14. Parlaktas BS, Ozyurt B, Ozyurt H, et al. (2008): Levels of oxidative stress parameters and the protective effects of melatonin in psychosis model rat testis. Asian Journal of Andrology, 10(2), 259-65.
- 15. Reddy KP, Madhu P, Reddy S (2016): Protective effects of resveratrol against cisplatin-induced testicular and epididymal toxicity in rats. Food and Chemical Toxicology, 91, 65-72.
- 16. Sakr HF, Abbas AM, Elsamanoudy AZ, et al. (2015): Effect of fluoxetine and resveratrol on testicular functions and oxidative stress in a rat model of chronic mild stress-induced depression. Journal of Physiology and Pharmacology, 66(4), 515-527.
- 17. Saleh YS, Sawiress FA, Tony MA, et al. (2015): Protective role of some feed additives against dizocelpine induced oxidative stress in testes of rabbit bucks. Journal of Agricultural Science, 7(10), 239-252.
- 18. Sonmez M, Turk G, Yuce A (2005): The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63(7), 2063-2072.
- 19. Storto M, Sallese M, Salvatore L, et al. (2001): Expression of metabotropic glutamate receptors in the rat and human testis. Journal of Endocrinology, 170(1), 71-78.
- 20. Takarada T, Hinoi E, Balcar VJ, et al. (2004): Possible expression of functional glutamate transporters in the rat testis. Journal of Endocrinology, 181, 233-244.
- 21. Tas M, Saruhan BG, Kurt D, et al. (2010): Protective role of lycopene on aflatoxin b1 induced changes sperm characteristics and testicular damages in rats. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(4), 597-604.
- 22. Xiu Y, Kong XR, Zhang L, et al. (2014): White matter injuries induced by MK-801 in a mouse model of schizophrenia based on NMDA antagonism. The Anatomical Record (Hoboken), 297, 1498-1507.
- 23. Xiu Y, Kong XR, Zhang L, et al. (2015): The myelinated fiber loss in the corpus callosum of mouse model of schizophrenia induced by MK-801. Journal of Psychiatric Research, 63, 132-140.
- 24. Yu J, Qi D, Xing M, et al. (2011): MK-801 induces schizophrenic behaviors through downregulating Wnt signaling pathways in male mice. Brain Research, 1385, 281-292.
Abstract
Günümüzde MK-801’in şizofreniye sebep olan dozlarının testiste oksidatif strese de neden olduğu ortaya çıkmıştır. Bu çalışma fare testislerinde MK-801 kullanılarak ortaya çıkartılan oksidatif strese karşı resveratrolün koruyucu etkisini araştırmak amacıyla yapılmıştır. Testisler ağırlık, toplam oksidan-antioksidan seviyeleri, seminifer tübüllerin çapları ve epitel dokularının yükseklikleri, patolojik ve epididimal sperm motilitesi yönünden değerlendirilmiştir. Bu amaçla 24 fare alınarak grup başına 6’şar fareden oluşan 4 grup oluşturulmuştur. Grup I, kontrolden oluşmuştur ve bu gruba intraperitoneal yolla %0,9 FTS (10ml/kg) verilmiştir. Grup II’ye i.p. yolla MK-801 (1mg/kg), Grup III’e i.p. yolla MK-801 (1mg/kg) ve resveratrol (40mg/kg), Grup IV’e de i.p. olarak resveratrol (40mg/kg) verilmiştir. Uygulamalar on dört gün boyunca sürmüştür. Bu sürenin sonunda testisler çıkartılmış ve yapılan incelemeler neticesinde testiste ikinci grupta MK-801’in oksidatif strese sebep olduğu saptanmış, testis ağırlığında azalma, sperm motilitesinde düşme ve tübüllerde patolojik değişiklikler olduğu görülmüştür. Resveratrolün ise oksidatif strese karşı testisi koruduğu ortaya konulmuştur. Ayrıca resveratrol grubunda kontrole göre sperm motilitesinin arttığı gözlenmiştir. Sonuç olarak MK-801’in testiste oksidatif strese sebep olduğu ve resveratrolün de koruyucu etkisinin bulunduğu ortaya çıkarılmıştır.
Keywords
References
- 1. Araghi A, Tabari AM, Golshahi H (2017): Protective effect of carvacrol on ketamine induced testicular damage in mouse model of schizophrenia. Journal of HerbMed Pharmacology, 6(3), 100-106.
- 2. Cui X, Jing X, Wu X, et al. (2016): Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity. Molecular Medicine Reports, 14(5), 4659-4665.
- 3. Erel O (2004): A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(2), 112-119.
- 4. Erel O (2005): A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-1111.
- 5. Faid I, Al-Hussaini H, Kilarkaje N (2015): Resveratrol alleviates diabetes-induced testicular dysfunction by inhibiting oxidative stress and c-Jun N-terminal kinase signaling in rats. Toxicology and Applied Pharmacology, 289(3), 482-94.
- 6. Hu JH, Yang N, Ma YH, et al. (2004): Identification of glutamate transporters and receptors in mouse testis. Acta Pharmacologica Sinica, 25(3), 366-371.
- 7. Kovacic P, Somanathan R (2010): Clinical physiology and mechanism of dizocilpine (MK-801) Electron transfer, radicals, redox metabolites and bioactivity. Oxidative Medicine and Cellular Longevity, 3(1), 13-22.
- 8. Kruk-Slomka M, Budzynska B, Slomka T, et al. (2016): The influence of the CB1 receptor ligands on the schizophrenia-like effects in mice induced by MK-801. Neurotoxicity Research, 30(4), 658-676.
- 9. Lewis DA, Lieberman JA (2000): Catching up on Schizophrenia: Natural history and neurobiology. Neuron, 28(2), 325-34.
- 10. Mendes TB, Paccola CC, de Oliveira Neves FM, et al. (2016): Resveratrol improves reproductive parameters of adult rats varicocelized in peripuberty. Reproduction, 152(1), 23-35.
- 11. Ourique GM, Finamor IA, Saccol EM, et al. (2013): Resveratrol improves sperm motility, prevents lipid peroxidation and enhances antioxidant defences in the testes of hyperthyroid rats. Reproductive Toxicology, 37, 31-39.
- 12. Ozyurt B, Parlaktas BS, Ozyurt H, et al. (2007): A preliminary study of the levels of testis oxidative stress parameters after MK-801-induced experimental psychosis model: protective effects of CAPE. Toxicology, 230, 83-89.
- 13. Pankevich DE, Davis M, Altevogt BM (2011): Glutamate-Related biomarkers in drug development for disorders of the nervous system: Workshop summary. National Academies Press (US), Washington (DC). pp. 5.
- 14. Parlaktas BS, Ozyurt B, Ozyurt H, et al. (2008): Levels of oxidative stress parameters and the protective effects of melatonin in psychosis model rat testis. Asian Journal of Andrology, 10(2), 259-65.
- 15. Reddy KP, Madhu P, Reddy S (2016): Protective effects of resveratrol against cisplatin-induced testicular and epididymal toxicity in rats. Food and Chemical Toxicology, 91, 65-72.
- 16. Sakr HF, Abbas AM, Elsamanoudy AZ, et al. (2015): Effect of fluoxetine and resveratrol on testicular functions and oxidative stress in a rat model of chronic mild stress-induced depression. Journal of Physiology and Pharmacology, 66(4), 515-527.
- 17. Saleh YS, Sawiress FA, Tony MA, et al. (2015): Protective role of some feed additives against dizocelpine induced oxidative stress in testes of rabbit bucks. Journal of Agricultural Science, 7(10), 239-252.
- 18. Sonmez M, Turk G, Yuce A (2005): The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63(7), 2063-2072.
- 19. Storto M, Sallese M, Salvatore L, et al. (2001): Expression of metabotropic glutamate receptors in the rat and human testis. Journal of Endocrinology, 170(1), 71-78.
- 20. Takarada T, Hinoi E, Balcar VJ, et al. (2004): Possible expression of functional glutamate transporters in the rat testis. Journal of Endocrinology, 181, 233-244.
- 21. Tas M, Saruhan BG, Kurt D, et al. (2010): Protective role of lycopene on aflatoxin b1 induced changes sperm characteristics and testicular damages in rats. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(4), 597-604.
- 22. Xiu Y, Kong XR, Zhang L, et al. (2014): White matter injuries induced by MK-801 in a mouse model of schizophrenia based on NMDA antagonism. The Anatomical Record (Hoboken), 297, 1498-1507.
- 23. Xiu Y, Kong XR, Zhang L, et al. (2015): The myelinated fiber loss in the corpus callosum of mouse model of schizophrenia induced by MK-801. Journal of Psychiatric Research, 63, 132-140.
- 24. Yu J, Qi D, Xing M, et al. (2011): MK-801 induces schizophrenic behaviors through downregulating Wnt signaling pathways in male mice. Brain Research, 1385, 281-292.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | April 5, 2019 |
| Published in Issue | Year 2019Volume: 66 Issue: 2 |