Letter to Editor
Investigation of extended-spectrum beta-laktamase activity and antibiotic resistance profile ofEscherichia coli strains isolated from bovine mastitis
Abstract
The aim of this study was to investigate the extended-spectrum beta-laktamase activity (ESBL) and antibiotic resistance profile of 92 Escherichia coli strains isolated from bovine mastitic milk samples. Milk samples were obtained from dairy farms in Ankara, Balıkesir and Çorum. ESBL screening test, ESBL phenotypic confirmation test and in vitro susceptibility test for twelve antibiotics were performed with Kirby Bauer disc diffusion method in E. coli strains. Among the E. coli strains, respectively the highest resistance rates were detected against to erythromycin (69.6%), ampicillin (39.1%), tetracycline (34.8%), nalidixic acid (25.0%), chloramphenicol (22.8%), trimethoprim-sulfamethoxazole and amoxicillin-clavulanic acid (21.7%). Of all strains, 25.0% were found susceptible to all antibiotics and %54.3 of E. coli were resistant to two or more antibiotics.This was the first study that ESBL activity of E. coli isolated from mastitis was investigated in our country. However among the E. coli isolates, ESBL activity was not detected. This study showed that high rates of multidrug resistance developed in E. coli strains caused mastitis
References
- Aarestrup FM (1999): Association between the consumption of antimicrobial agents in animal husbandry and the occurrence of resistant bacteria among food animals. Int J Antimicrob Agents, 12, 279–285.
- Baştan, A (2002): İneklerde meme hastalıkları, 41-50, Hatipoğlu Yayınevi. Ankara.
- Bauer AU, Kirby WM, Sherris JC, Tack M (1966): Antibiotic susceptibility testing by a standardized single disc method. J Clin Pathol, 45, 493-494.
- Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Öst M, Waller KP (2009): Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol, 136, 142- 149.
- Blanc V, Mesa R, Saco M, Lavilla S, Prats G, Mirò E, Navarro F, Cortés P, Llastera M (2006): ESBL- and plasmidic C β-lactamase-producing E. coli strains isolated from poultry, pig and rabbit farms. Vet Microbiol, 118, 299–304.
- Botrel MA, Haenni M, Morignat E, Sulpice P, Madec JY, Calavas D (2010): Distribution and antimicrobial resistance of clinical and subclinical mastitis pathogens in dairy cows in Rhône-Alpes, France. Foodborne Pathog Dis, 7, 479-487.
- Briñas L, Moreno MA, Teshager T, Sáenz Y, Porrero MC, Domínguez L, Torres C (2005): Monitoring and characterization of extended-spectrum β-lactamases in Escherichia coli strains from healty and sick animals in Spain in 2003. Antimicrob Agents Chemother, 49, 1262- 1264.
- Burvenıch C, Van Merrıs V, Mehrzad J, Diez-Fraile A, Duchateau L (2003): Severity of E. coli mastitis is mainly determined by cow factors. Vet. Res, 34, 521–564.
- Bush K (2001): New β-lactamases in Gram negative bacteria:diversity and impact on selection of antimicrobial therapy. Clin İnfect Dis, 3, 1085-1089.
- CLSI (2007): Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing. 17th informational supplement. Approved Standard, MS100-S17, Wayne, PA, USA.
- Diker S (1984): Koliform mastitisler. 147-154. In: I. Mastitis Semineri. Lalahan Veteriner Zootekni Araştırma Enstitüsü Yetiştirme ve Deneme Çiftliği Müdürlüğü Ofset Tesisleri, Ankara.
- Guérin-Faublée V, Carret G, Houffschmitt P (2003): In vitro activity of 10 antimicrobial agents against bacteria isolated from cows with clinical mastitis. Vet Rec, 152, 466-471.
- Güdücüoğlu H, Baykal S, İzci H, Berktaş M (2007): Genişlemiş spektrumlu beta laktamaz (GSBL) üreten Escherichia coli ve Klebsiella pneumoniae suşlarının antibiyotiklere direnci. ANKEM Derg, 21, 155-160.
- Hammerum AM, Heuer OE (2009): Human health hazards from antimicrobial resistant Escherichia coli of animal origin. Clin İnfect Dis, 48, 916-921.
- Jones TO (1999): E. coli mastitis-The past, the present and the future. Proceedings of the British Mastitis Conference, 62-72.
- Kalmus P, Aasmäe B, Kärssin A, Orro T, Kask K (2011): Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet Scand, 53, 1-7.
- Kojima A, Ishii Y, Ishihara K, Esaki H, Asai T, Oda C, Tamura Y, Takahashi T, Yamaguchi K (2005): Extended spectrum β-lactamase producing Escherichia coli strains isolated from farm animals from 1999 to 2002: report from the japanes veterinary animal resistance monitoring program. Antimicrob Agents Chemother, 49, 3533-3537.
- Lehtolainen T, Shwimmer A, Shpigel NY, Honkanen- Buzalski T, Pyörälä S (2003): In vitro antimicrobial susceptibility of Escherichia coli isolates from clinical bovine mastitis in Finland and Israel. J Dairy Sci, 86:3927-3932.
- Livermore DM (1995): β-lactamases in laboratory and clinical resistance. Clin Microbiol Rev, 8, 557–584.
- Locatelli C, Caronte I, Scaccabarozzi L, Migliavacca R, Pagani L, Moroni P (2009): Extended-spectrum β- lactamase production in E. coli strains isolated from clinical bovine mastitis. Vet Res Commun, 33, 141-144.
- Makovec JA, Ruegg PL (2003): Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994-2001). J Am Vet Med, 222, 1582-1589.
- Muckle CA, Prescott JF, Johston R (1986): Susceptibility of Escherichia coli from bovine mastitis to new antimicrobial drugs. Can J Vet Res, 50, 543-544.
- Srinivasan V, Gillespie BE, Lewis MJ, Nguyen LT, Headrick SI, Schukken YH, Oliver SP (2007): Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli isolated from dairy cows with mastitis. Vet Microbiol, 124, 319-328.
- Sumathi BR, Amitra RG, Krishnappa G (2008): Antibiogram profile based dendogram analysis of Escherichia coli serotypes isolated from bovine mastitis. Vet World, 1, 37-39.
- Tel OY, Keskin O, Zonturlu AK, Arserim Kaya NB (2009): Şanlıurfa yöresinde subklinik mastitislerin görülme oranı, aerobik bakteri izolasyonu ve duyarlı antibiyotiklerin belirlenmesi. F Ü Sağ Bil Vet Derg, 23, 101-106.
Sığır mastitislerinden izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz aktivitesi ve antibiyotik dirençlilik profilinin incelenmesi
Abstract
Bu çalışmada Ankara, Balıkesir ve Çorum’daki süt işletmelerinden sağlanan mastitis şüpheli sütlerden izole edilen 92 adet E. coli suşunda genişlemiş spektrumlu beta-laktamaz (GSBL) aktivitesi ve antibiyotik direnç profilinin araştırılması amaçlandı. E. coli suşlarında Kirby Bauer disk diffüzyon yöntemi ile GSBL tarama testi, fenotipik GSBL doğrulama testi ve 12 adet antibiyotik için in vitro duyarlılık testleri yapıldı. İncelenen E. coli suşlarında en yüksek dirençlilik oranları sırasıyla eritromisine (%69.6), ampisiline (%39.1), tetrasikline (%34.8), nalidiksik aside (%25.0), kloramfenikole (%22.8), trimetoprim-sülfametaksazole (%21.7) ve amoksisilin klavulonik aside (%21.7) karşı bulunurken, suşların %25.0’inin kullanılan tüm antibiyotiklere duyarlı olduğu saptandı. Ayrıca E. coli suşlarının %54.3’ünün iki veya daha fazla sayıda antibiyotiğe dirençli olduğu belirlendi. Bu çalışma ile ülkemizde mastitis orijinli E. coli suşlarında ilk kez GSBL aktivitesi araştırıldı. Ancak, incelenen E. coli suşlarında GSBL aktivitesi saptanmadı. Çalışmada elde edilen bulgular mastitise neden olan E. coli suşlarında yüksek oranda çoğul ilaç direncinin geliştiğini gösterdi
Keywords
References
- Aarestrup FM (1999): Association between the consumption of antimicrobial agents in animal husbandry and the occurrence of resistant bacteria among food animals. Int J Antimicrob Agents, 12, 279–285.
- Baştan, A (2002): İneklerde meme hastalıkları, 41-50, Hatipoğlu Yayınevi. Ankara.
- Bauer AU, Kirby WM, Sherris JC, Tack M (1966): Antibiotic susceptibility testing by a standardized single disc method. J Clin Pathol, 45, 493-494.
- Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Öst M, Waller KP (2009): Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol, 136, 142- 149.
- Blanc V, Mesa R, Saco M, Lavilla S, Prats G, Mirò E, Navarro F, Cortés P, Llastera M (2006): ESBL- and plasmidic C β-lactamase-producing E. coli strains isolated from poultry, pig and rabbit farms. Vet Microbiol, 118, 299–304.
- Botrel MA, Haenni M, Morignat E, Sulpice P, Madec JY, Calavas D (2010): Distribution and antimicrobial resistance of clinical and subclinical mastitis pathogens in dairy cows in Rhône-Alpes, France. Foodborne Pathog Dis, 7, 479-487.
- Briñas L, Moreno MA, Teshager T, Sáenz Y, Porrero MC, Domínguez L, Torres C (2005): Monitoring and characterization of extended-spectrum β-lactamases in Escherichia coli strains from healty and sick animals in Spain in 2003. Antimicrob Agents Chemother, 49, 1262- 1264.
- Burvenıch C, Van Merrıs V, Mehrzad J, Diez-Fraile A, Duchateau L (2003): Severity of E. coli mastitis is mainly determined by cow factors. Vet. Res, 34, 521–564.
- Bush K (2001): New β-lactamases in Gram negative bacteria:diversity and impact on selection of antimicrobial therapy. Clin İnfect Dis, 3, 1085-1089.
- CLSI (2007): Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing. 17th informational supplement. Approved Standard, MS100-S17, Wayne, PA, USA.
- Diker S (1984): Koliform mastitisler. 147-154. In: I. Mastitis Semineri. Lalahan Veteriner Zootekni Araştırma Enstitüsü Yetiştirme ve Deneme Çiftliği Müdürlüğü Ofset Tesisleri, Ankara.
- Guérin-Faublée V, Carret G, Houffschmitt P (2003): In vitro activity of 10 antimicrobial agents against bacteria isolated from cows with clinical mastitis. Vet Rec, 152, 466-471.
- Güdücüoğlu H, Baykal S, İzci H, Berktaş M (2007): Genişlemiş spektrumlu beta laktamaz (GSBL) üreten Escherichia coli ve Klebsiella pneumoniae suşlarının antibiyotiklere direnci. ANKEM Derg, 21, 155-160.
- Hammerum AM, Heuer OE (2009): Human health hazards from antimicrobial resistant Escherichia coli of animal origin. Clin İnfect Dis, 48, 916-921.
- Jones TO (1999): E. coli mastitis-The past, the present and the future. Proceedings of the British Mastitis Conference, 62-72.
- Kalmus P, Aasmäe B, Kärssin A, Orro T, Kask K (2011): Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet Scand, 53, 1-7.
- Kojima A, Ishii Y, Ishihara K, Esaki H, Asai T, Oda C, Tamura Y, Takahashi T, Yamaguchi K (2005): Extended spectrum β-lactamase producing Escherichia coli strains isolated from farm animals from 1999 to 2002: report from the japanes veterinary animal resistance monitoring program. Antimicrob Agents Chemother, 49, 3533-3537.
- Lehtolainen T, Shwimmer A, Shpigel NY, Honkanen- Buzalski T, Pyörälä S (2003): In vitro antimicrobial susceptibility of Escherichia coli isolates from clinical bovine mastitis in Finland and Israel. J Dairy Sci, 86:3927-3932.
- Livermore DM (1995): β-lactamases in laboratory and clinical resistance. Clin Microbiol Rev, 8, 557–584.
- Locatelli C, Caronte I, Scaccabarozzi L, Migliavacca R, Pagani L, Moroni P (2009): Extended-spectrum β- lactamase production in E. coli strains isolated from clinical bovine mastitis. Vet Res Commun, 33, 141-144.
- Makovec JA, Ruegg PL (2003): Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994-2001). J Am Vet Med, 222, 1582-1589.
- Muckle CA, Prescott JF, Johston R (1986): Susceptibility of Escherichia coli from bovine mastitis to new antimicrobial drugs. Can J Vet Res, 50, 543-544.
- Srinivasan V, Gillespie BE, Lewis MJ, Nguyen LT, Headrick SI, Schukken YH, Oliver SP (2007): Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli isolated from dairy cows with mastitis. Vet Microbiol, 124, 319-328.
- Sumathi BR, Amitra RG, Krishnappa G (2008): Antibiogram profile based dendogram analysis of Escherichia coli serotypes isolated from bovine mastitis. Vet World, 1, 37-39.
- Tel OY, Keskin O, Zonturlu AK, Arserim Kaya NB (2009): Şanlıurfa yöresinde subklinik mastitislerin görülme oranı, aerobik bakteri izolasyonu ve duyarlı antibiyotiklerin belirlenmesi. F Ü Sağ Bil Vet Derg, 23, 101-106.
There are 25 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA97YG82FK |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2012 |
| Published in Issue | Year 2012Volume: 59 Issue: 2 |