Abstract
The purpose of the study was to evaluate erythrocyte indices and platelet indices in dogs with leishmaniasis (CL, L. infantum). CL was diagnosed by polymerase chain reaction, and confirmed by western blott in 54 dogs. These dogs were divided into three groups; asymptomatic (n=16), oligosymptomatic (n=16), and symptomatic (n=22). Leishmaniasis free 25 dogs were served as controls. Microcytic or normocytic-hypochromic anemia was observed in dogs with CL. There was not a significant difference on RDW (RBC distribution width) groups. PLT count was slightly lower in dogs with CL in all clinical stages (p<0.05), compared to controls. MPV (mean platelet volume) in dogs with CL was higher (P<0.05) compared with controls, and it was significantly higher in asymptomatic dogs. RBC (red blood cell) and platelet indices may be useful parameters during course of the leismaniasis. These indices may have diagnostic properties but are not enough to differentiate of the clinical stages of CL. Further comprehensive studies are needed to clinical use of RBC and platelets indices in canine practice
Keywords
References
- Baneth G, Aroch I (2008): Canine leishmaniasis: A diagnostic and clinical challenge. Vet J, 175, 14-15.
- Bessman JD, Williams LJ, Gilmer PR (1981): Mean platelet volume. The inverse relation of platelet size and count in normal subjects, and an artifact of other particles. Am J Clin Pathol, 76, 289–293.
- Corona M, Ciaramella P, Pelagalli A, Cortese L, Pero ME, Santoro D, Lombardi P (2004): Haemostatic disorders in dogs naturally infected by Leishmania infantum. Vet Res Commun, 28, 331-334.
- da Costa-Val AP, Cavalcanti RR, de Figueiredo Gontijo N, Michalick MS, Alexander B, Williams P, Melo MN (2007): Canine visceral leishmaniasis: relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpis infectivity. Vet J, 174, 636-643.
- Domínguez M, Toraño A (2001): Leishmania immune adherence reaction in vertebrates. Parasite Immunol, 23, 259-265.
- Grindem CB, Breitschwerdt EB, Corbett WT, Jans HE (1991): Epidemiologic survey of thrombocytopenia in dogs: a report on 987 cases. Vet Clin Path, 20, 38-43
- Kager PA, Rees PH. (1986): Haematological investigations in visceral leishmaniasis. Trop Geogr Med, 38, 371-379.
- Kaito K, Otsubo H, Usui N, Yoshida M, Tanno J, Kurihara E, Matsumoto K, Hirata R, Domitsu K, Kobayashi M (2005): Platelet size deviation width, platelet large cell ratio, and mean platelet volume have sufficient sensitivity and specificity in the diagnosis of immune thrombocytopenia. Brit J Haematol, 128, 698–702.
- Lomtadze ML, Khochava MA, Shalamberidze IA, Shilakadze MA, Dzhokhtaberidze TG (2005): Functional status of haemostasis system in patients with visceral leishmaniasis. Georgian Med News, 128, 59-62.
- Mahajan V, Marwaha RK (2007): Immune mediated hemolysis in visceral leishmaniasis. J Trop Pediatr, 53, 284-286.
- Mancianti F, Meciani N (1988): Specific serodiagnosis of canine leishmaniasis by indirect immunofluorescence, indirect hemagglutination, and counter immunoelectrophoresis. Am J Vet Res, 49, 1409-1411.
- Michalsky EM, Rocha MF, da Rocha Lima AC, França-Silva JC, Pires MQ, Oliveira FS, Pacheco RS, dos Santos SL, Barata RA, Romanha AJ, Fortes-Dias CL, Dias ES (2007): Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies. Vet Parasitol, 147, 67-76.
- Moreno P (1999): Evaluation of secondary haemostasis in canine leishmaniasis. Vet Rec, 144, 169–171.
- Moreno P, Lucena R, Ginel PJ (1998): Evaluation of primary haemostasis in canine leishmaniasis. Vet Rec, 24, 142, 81-83.
- Moritz A,Walcheck BK, Weiss DJ (2005): Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease. Am J Vet Res, 66, 325–329
- Park Y, Schoene N, Harris W (2002): Mean platelet volume as an indicator of platelet activation: methodological issues. Platelets, 13, 301-306.
- Reis AB, Teixeira-Carvalho A, Giunchetti RC, Guerra LL, Carvalho MG, Mayrink W, Genaro O, Corrêa- Oliveira R, Martins-Filho OA (2006): Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clin Exp Immunol, 146, 303-311.
- Slappendel RJ, Greene CE (1990): Leishmaniasis, 769– 777. In: Greene CE (Ed), Infectious Diseases of the Dog and Cat, W.B. Saunders, Philadelphia.
- Solano-Gallego L, Riera C, Roura X, Iniesta L, Gallego M, Valladares JE, Fisa R, Castillejo S, Alberola J, Ferrer L, Arboix M, Portús M (2001): Leishmania infantum-specific IgG, IgG1 and IgG2 antibody responses in healthy and ill dogs from endemic areas. Evolution in the course of infection and after treatment. Vet Parasitol, 96, 265-276.
Abstract
Bu çalışmanın amacı, Canine Leismaniazis (CL, L. infantum) tanısı konan köpeklerde eritrosit ve platelet indekslerindeki değişimleri değerlendirmektir. Bu çalışmanın materyalini, farklı ırk, yaş ve cinsiyetteki 54 köpek oluşturdu. CL (L.infantum) tanısı, PCR (poymerase chain reaction) yöntemiyle kondu, Western blott yöntemiyle de teyit edildi. Çalışmayı oluşturan köpekler üç gruba ayrıldı: Bu gruplar, asemptomatik (n:16), oligosemptomatik (n:16) ve semptomatik (n:22) köpeklerin (en az üç karakteristik bulguyu taşıyan köpekler) oluşturduğu gruplar olarak düzenlendi. Ayrıca 25 adet sağlıklı köpek kontrol grubunu oluşturdu. CL’lı köpeklerde eritrositlerin morfolojik değerlendirmesinde mikrositik, normositik ve hipokromik oldukları saptandı. Gruplar arasında RDW değeri açısından bir fark saptanmadı. PLT değerinin bütün klinik safhalarda hafif düşük (p<0.05) olduğu belirlendi. MPV değeri CL köpeklerde sağlıklı köpeklere göre (p<0.05) önemli derecede yüksekti. Ayrıca diğer gruplara göre özellikle asemptomatik grupta daha yüksek olduğu saptandı. Bu çalışmada, eritrosit ve platelet indekslerinin CL’nın seyri esnasında kullanışlı parametreler olarak değerlendirilebileceği, klinik safhaların ayrımında kullanışlı olmamakla birlikte diagnostik uygunluğa sahip olduğu kanısına varıldı. İleride klinik pratikte, eritrosit ve platelet indekslerinin klinik kullanımına ilişkin daha ayrıntılı çalışmalara ihtiyaç duyulmaktadır
Keywords
References
- Baneth G, Aroch I (2008): Canine leishmaniasis: A diagnostic and clinical challenge. Vet J, 175, 14-15.
- Bessman JD, Williams LJ, Gilmer PR (1981): Mean platelet volume. The inverse relation of platelet size and count in normal subjects, and an artifact of other particles. Am J Clin Pathol, 76, 289–293.
- Corona M, Ciaramella P, Pelagalli A, Cortese L, Pero ME, Santoro D, Lombardi P (2004): Haemostatic disorders in dogs naturally infected by Leishmania infantum. Vet Res Commun, 28, 331-334.
- da Costa-Val AP, Cavalcanti RR, de Figueiredo Gontijo N, Michalick MS, Alexander B, Williams P, Melo MN (2007): Canine visceral leishmaniasis: relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpis infectivity. Vet J, 174, 636-643.
- Domínguez M, Toraño A (2001): Leishmania immune adherence reaction in vertebrates. Parasite Immunol, 23, 259-265.
- Grindem CB, Breitschwerdt EB, Corbett WT, Jans HE (1991): Epidemiologic survey of thrombocytopenia in dogs: a report on 987 cases. Vet Clin Path, 20, 38-43
- Kager PA, Rees PH. (1986): Haematological investigations in visceral leishmaniasis. Trop Geogr Med, 38, 371-379.
- Kaito K, Otsubo H, Usui N, Yoshida M, Tanno J, Kurihara E, Matsumoto K, Hirata R, Domitsu K, Kobayashi M (2005): Platelet size deviation width, platelet large cell ratio, and mean platelet volume have sufficient sensitivity and specificity in the diagnosis of immune thrombocytopenia. Brit J Haematol, 128, 698–702.
- Lomtadze ML, Khochava MA, Shalamberidze IA, Shilakadze MA, Dzhokhtaberidze TG (2005): Functional status of haemostasis system in patients with visceral leishmaniasis. Georgian Med News, 128, 59-62.
- Mahajan V, Marwaha RK (2007): Immune mediated hemolysis in visceral leishmaniasis. J Trop Pediatr, 53, 284-286.
- Mancianti F, Meciani N (1988): Specific serodiagnosis of canine leishmaniasis by indirect immunofluorescence, indirect hemagglutination, and counter immunoelectrophoresis. Am J Vet Res, 49, 1409-1411.
- Michalsky EM, Rocha MF, da Rocha Lima AC, França-Silva JC, Pires MQ, Oliveira FS, Pacheco RS, dos Santos SL, Barata RA, Romanha AJ, Fortes-Dias CL, Dias ES (2007): Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies. Vet Parasitol, 147, 67-76.
- Moreno P (1999): Evaluation of secondary haemostasis in canine leishmaniasis. Vet Rec, 144, 169–171.
- Moreno P, Lucena R, Ginel PJ (1998): Evaluation of primary haemostasis in canine leishmaniasis. Vet Rec, 24, 142, 81-83.
- Moritz A,Walcheck BK, Weiss DJ (2005): Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease. Am J Vet Res, 66, 325–329
- Park Y, Schoene N, Harris W (2002): Mean platelet volume as an indicator of platelet activation: methodological issues. Platelets, 13, 301-306.
- Reis AB, Teixeira-Carvalho A, Giunchetti RC, Guerra LL, Carvalho MG, Mayrink W, Genaro O, Corrêa- Oliveira R, Martins-Filho OA (2006): Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clin Exp Immunol, 146, 303-311.
- Slappendel RJ, Greene CE (1990): Leishmaniasis, 769– 777. In: Greene CE (Ed), Infectious Diseases of the Dog and Cat, W.B. Saunders, Philadelphia.
- Solano-Gallego L, Riera C, Roura X, Iniesta L, Gallego M, Valladares JE, Fisa R, Castillejo S, Alberola J, Ferrer L, Arboix M, Portús M (2001): Leishmania infantum-specific IgG, IgG1 and IgG2 antibody responses in healthy and ill dogs from endemic areas. Evolution in the course of infection and after treatment. Vet Parasitol, 96, 265-276.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA95CZ55ZJ |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2011 |
| Published in Issue | Year 2011Volume: 58 Issue: 3 |