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Mastitislerden izole edilen enterokokların moleküler tiplendirilmesi

Year 2011, Volume: 58 Issue: 1, 17 - 20, 01.03.2011

Seyda Cengiz Oya Tekin Mehmet Akan

https://doi.org/10.1501/Vetfak_0000002443

Abstract

İnek mastitislerinde enterokok etkenlerinin varlığını belirlemek ve izole edilen suşları moleküler tekniklerle tiplendirmek amacıyla yapılan bu çalışmada, Ankara ve Balıkesir illerindeki süt işletmelerinden sağlanan sütlerden izole edilen 75 adet enterokok suşu incelendi. Moleküler tiplendirme Restriksiyon Enzimi Uzunluk Polimorfizmleri- Polimeraz Zincir Reaksiyonu (RFLP-PCR) ile yapıldı. Bu yöntemle 61 adet etken Enterococcus faecium ve 14 adet etken ise Enterococcus avium olarak identifiye edildi. İzolatların genotiplendirilmesi amacıyla yapılan Rastgele Çoğaltılmış Polimorfik DNA- Polimeraz Zincir Reaksiyonu sonuçlarına göre Enterococcus faecium suşlarında 10 adet, Enterococcus avium suşlarında ise 7 adet farklı profil görüntülendi. Bu bulgular enterokok etkenlerinin inek mastitislerindeki önemini ve rolünü göstermektedir

Keywords

Enterokok mastitis moleküler tiplendirme

References

  • Akan M (2006): Enterococcus infeksiyonları. 27-29. In: N Aydın, J Paracıkoğlu (Ed) , Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). İlke Emek Yayınları, Ankara.
  • Aaestrup F, Butaye P, Witte W (2002): Nonhuman reservoirs of Enterococci. 55-100. In: Gilmore MS (Ed) The Enterococci (Pathogenesis, Molecular Biology and Antibiotic Resistance) ASM Press, USA
  • Bensalah F, Flores MJ, Mouats A (2006): A rapid PCR based method to distinguish between Enterococcus species by using degenerate and species-specific Sod A gene primers. Afr. J. Biotechnol. 5, 607-702
  • Charles MAPF, Albrecth BMS, Nuham KY, Marc V, Jean S, Wilhelm HH (2001): Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl. Environ. Microbiol. 67,4385- 4389
  • Cocconcelli PS, Porro D, Galandini S, Senini L (1995): Development of RAPD protocol for typing of strains of lactic acid bacteria and Enterococci Lett. App. Microbiol. 21, 376-379
  • Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F (1999): Identification of aesculin- hydrolyzing Streptococci, Lactococci, Aerococci and Enterococci from subclinical intramammary infections in dairy cows. Vet. Microbiol. 70, 87-94
  • Franzetti L, Pompei M, Scarpellini M, Galli A (2004): Phenotypic and genotypic characterization of Enterococcus spp. of different origins. Current Microbiol. 49, 255–260
  • Hardie JM, Whiley RA (1997): Classification and overview of the genera Streptococcus and Enterococcus. J. Appl. Microbiol. Symp. Supp. 83, 1-11
  • Garrity GM (2001): Bergey’s manual of systematic bacteriology Vol: 5 Springer-Verlag, New York. http://cme.msu.edu/Bergeys/
  • Gillespie BE, Jayarao BM, Oliver SP (1997): Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting. J. Dairy Sci. 80, 471-476
  • Jayarao BM, Dore JJE, Oliver SP (1992): Restriction fragment length polymorphism analysis of 16S ribosomal DNA Streptococcus and Enterococcus species of bovine origin. J. Clin. Microbiol. 30, 2235- 2240
  • Jayarao BM, Oliver SP, Matthews KR, King SH. (1991) Comparative evaluation of Vitek gram-positive identification system and API Rapid Strep system for identification of Streptococcus species of bovine origin. Vet Microbiol 26, 301-308
  • Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Dupre I, Sechi LA (2003): Comparison of incidence of virulence determinants and antibiotics resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int. J. Food. Microbiol. 88, 291-304
  • McDonald TJ, McDonald JS (1976): Streptococci isolated from bovine intramammary infections. Am. J. Vet. Res. 37, 377-81
  • Nam HM, Lim SK, Moon JS, Kang HM, Kim JM, Jang KC, Kang MI, Joo YS, Jung SC (2009) Antimicrobial resistance of Enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health. [Published online: Dec 23,2009,10.00 pm] DOI: 10.1111/J.1863-2378.2009.01307x
  • Park YK, Koo HC, Kim SH, Hwang SY, Jung WK, Kim JM, Shin S, Kim RT, Park YH (2007): The analysis of milk components and pathogenic bacteria ısolated from bovine raw milk in Korea. J. Dairy Sci. 90, 5405–5414
  • Petersson-Wolfe CS, Wolf SL, Hogan JS (2007): In vitro growth of Enterococci of bovine origin in bovine mammary secretions from various stages of lactation. J. Dairy Sci. 90, 4246-4231
  • Petersson-Wolfe CS, Adams S, Wolf SL, Hogan JS (2007): Genomic typing of Enterococci isolated from bovine mammary glands and environmental sources. J. Dairy Sci. 91,615–619
  • Petersson-Wolfe, CS (2006): A study of the occurance, phenotypic and genotypic diversity and both in vitro and in vivo growth response of Enterococcus spp. isolated from bovine origin. Ohio State University,USA.
  • Riboldi GP, Mattos EP, Frazzon APG, Azevedo PA, Frazzon J (2008): Phenotypic and genotypic heterogeneity of Enterococcus species isolated from food in Southern Brazil. J. Basic Microbiol. 48, 31-37
  • Smith KL, Hogan JS (2003): Environmental mastitis caused by species of Streptococcus and Enterococcus: Risk Factors and Control. Ohio Agricultural Research and Development Center The Ohio State University Wooster, OH USA http://cals.arizona.edu/extension/dairy/conference/proceedi ngs/environmental_mastitis.pdf accessed 08.09.2009
  • Tenhagen BA, Köster G, Wallmann J, Heuwieser W (2006): Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J. Dairy Sci. 89,2542–2551
  • Watts JL (1988): Characterization and identification of Streptococci isolated from bovine mammary glands. J. Dairy.Sci. 71,1616-1624
  • Wolfgang DR, Jayarao BM, Pillai SR, Sawant AA, Burns CM, Hutchinson LJ Farm management practices that influence the number and type of Streptococci and Streptococci-like organisms in dairy herds. The Pennslyvania State University. University Park, PA, USA http://vbs.psu.edu/ext/focus-areas/mastitis/bulk-tank milk/Resources/Farm%20Management%20Practices.pdf accessed 08.09.09

Molecular typing of Enterococcus spp. isolated from cow mastitis

Year 2011, Volume: 58 Issue: 1, 17 - 20, 01.03.2011

Seyda Cengiz Oya Tekin Mehmet Akan

https://doi.org/10.1501/Vetfak_0000002443

Abstract

The objectives of this study were to the determine the presence of Enterococcus spp. in cow mastitis and to characterize the isolated strains using molecular techniques. A total of 75 Enterococcus spp. isolated from dairy farms in Ankara and Balıkesir cities were examined. Sixty-one Enterococcus faecium and 14 Enterococcus avium strains were identified according to Restriction Fragment Length Polymorphism-Polymerase Chain Reaction results. Additionally different 10 and 7 Random Amplified Polymorphic DNA- Polymerase Chain Reaction profiles were detected in E. faecium and E. avium strains, respectively. These finding showed the importance and the role of Enterococcus spp. in cow mastitis

Keywords

Enterococcus mastitis molecular typing

References

  • Akan M (2006): Enterococcus infeksiyonları. 27-29. In: N Aydın, J Paracıkoğlu (Ed) , Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). İlke Emek Yayınları, Ankara.
  • Aaestrup F, Butaye P, Witte W (2002): Nonhuman reservoirs of Enterococci. 55-100. In: Gilmore MS (Ed) The Enterococci (Pathogenesis, Molecular Biology and Antibiotic Resistance) ASM Press, USA
  • Bensalah F, Flores MJ, Mouats A (2006): A rapid PCR based method to distinguish between Enterococcus species by using degenerate and species-specific Sod A gene primers. Afr. J. Biotechnol. 5, 607-702
  • Charles MAPF, Albrecth BMS, Nuham KY, Marc V, Jean S, Wilhelm HH (2001): Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl. Environ. Microbiol. 67,4385- 4389
  • Cocconcelli PS, Porro D, Galandini S, Senini L (1995): Development of RAPD protocol for typing of strains of lactic acid bacteria and Enterococci Lett. App. Microbiol. 21, 376-379
  • Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F (1999): Identification of aesculin- hydrolyzing Streptococci, Lactococci, Aerococci and Enterococci from subclinical intramammary infections in dairy cows. Vet. Microbiol. 70, 87-94
  • Franzetti L, Pompei M, Scarpellini M, Galli A (2004): Phenotypic and genotypic characterization of Enterococcus spp. of different origins. Current Microbiol. 49, 255–260
  • Hardie JM, Whiley RA (1997): Classification and overview of the genera Streptococcus and Enterococcus. J. Appl. Microbiol. Symp. Supp. 83, 1-11
  • Garrity GM (2001): Bergey’s manual of systematic bacteriology Vol: 5 Springer-Verlag, New York. http://cme.msu.edu/Bergeys/
  • Gillespie BE, Jayarao BM, Oliver SP (1997): Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting. J. Dairy Sci. 80, 471-476
  • Jayarao BM, Dore JJE, Oliver SP (1992): Restriction fragment length polymorphism analysis of 16S ribosomal DNA Streptococcus and Enterococcus species of bovine origin. J. Clin. Microbiol. 30, 2235- 2240
  • Jayarao BM, Oliver SP, Matthews KR, King SH. (1991) Comparative evaluation of Vitek gram-positive identification system and API Rapid Strep system for identification of Streptococcus species of bovine origin. Vet Microbiol 26, 301-308
  • Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Dupre I, Sechi LA (2003): Comparison of incidence of virulence determinants and antibiotics resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int. J. Food. Microbiol. 88, 291-304
  • McDonald TJ, McDonald JS (1976): Streptococci isolated from bovine intramammary infections. Am. J. Vet. Res. 37, 377-81
  • Nam HM, Lim SK, Moon JS, Kang HM, Kim JM, Jang KC, Kang MI, Joo YS, Jung SC (2009) Antimicrobial resistance of Enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health. [Published online: Dec 23,2009,10.00 pm] DOI: 10.1111/J.1863-2378.2009.01307x
  • Park YK, Koo HC, Kim SH, Hwang SY, Jung WK, Kim JM, Shin S, Kim RT, Park YH (2007): The analysis of milk components and pathogenic bacteria ısolated from bovine raw milk in Korea. J. Dairy Sci. 90, 5405–5414
  • Petersson-Wolfe CS, Wolf SL, Hogan JS (2007): In vitro growth of Enterococci of bovine origin in bovine mammary secretions from various stages of lactation. J. Dairy Sci. 90, 4246-4231
  • Petersson-Wolfe CS, Adams S, Wolf SL, Hogan JS (2007): Genomic typing of Enterococci isolated from bovine mammary glands and environmental sources. J. Dairy Sci. 91,615–619
  • Petersson-Wolfe, CS (2006): A study of the occurance, phenotypic and genotypic diversity and both in vitro and in vivo growth response of Enterococcus spp. isolated from bovine origin. Ohio State University,USA.
  • Riboldi GP, Mattos EP, Frazzon APG, Azevedo PA, Frazzon J (2008): Phenotypic and genotypic heterogeneity of Enterococcus species isolated from food in Southern Brazil. J. Basic Microbiol. 48, 31-37
  • Smith KL, Hogan JS (2003): Environmental mastitis caused by species of Streptococcus and Enterococcus: Risk Factors and Control. Ohio Agricultural Research and Development Center The Ohio State University Wooster, OH USA http://cals.arizona.edu/extension/dairy/conference/proceedi ngs/environmental_mastitis.pdf accessed 08.09.2009
  • Tenhagen BA, Köster G, Wallmann J, Heuwieser W (2006): Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J. Dairy Sci. 89,2542–2551
  • Watts JL (1988): Characterization and identification of Streptococci isolated from bovine mammary glands. J. Dairy.Sci. 71,1616-1624
  • Wolfgang DR, Jayarao BM, Pillai SR, Sawant AA, Burns CM, Hutchinson LJ Farm management practices that influence the number and type of Streptococci and Streptococci-like organisms in dairy herds. The Pennslyvania State University. University Park, PA, USA http://vbs.psu.edu/ext/focus-areas/mastitis/bulk-tank milk/Resources/Farm%20Management%20Practices.pdf accessed 08.09.09
There are 24 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA43JU97YD
Journal Section Research Article
Authors

Seyda Cengiz

Oya Tekin

Mehmet Akan

Publication Date March 1, 2011
Published in Issue Year 2011Volume: 58 Issue: 1

Cite

APA Cengiz, S., Tekin, O., & Akan, M. (2011). Molecular typing of Enterococcus spp. isolated from cow mastitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 58(1), 17-20. https://doi.org/10.1501/Vetfak_0000002443
AMA Cengiz S, Tekin O, Akan M. Molecular typing of Enterococcus spp. isolated from cow mastitis. Ankara Univ Vet Fak Derg. March 2011;58(1):17-20. doi:10.1501/Vetfak_0000002443
Chicago Cengiz, Seyda, Oya Tekin, and Mehmet Akan. “Molecular Typing of Enterococcus Spp. Isolated from Cow Mastitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58, no. 1 (March 2011): 17-20. https://doi.org/10.1501/Vetfak_0000002443.
EndNote Cengiz S, Tekin O, Akan M (March 1, 2011) Molecular typing of Enterococcus spp. isolated from cow mastitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58 1 17–20.
IEEE S. Cengiz, O. Tekin, and M. Akan, “Molecular typing of Enterococcus spp. isolated from cow mastitis”, Ankara Univ Vet Fak Derg, vol. 58, no. 1, pp. 17–20, 2011, doi: 10.1501/Vetfak_0000002443.
ISNAD Cengiz, Seyda et al. “Molecular Typing of Enterococcus Spp. Isolated from Cow Mastitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 58/1 (March 2011), 17-20. https://doi.org/10.1501/Vetfak_0000002443.
JAMA Cengiz S, Tekin O, Akan M. Molecular typing of Enterococcus spp. isolated from cow mastitis. Ankara Univ Vet Fak Derg. 2011;58:17–20.
MLA Cengiz, Seyda et al. “Molecular Typing of Enterococcus Spp. Isolated from Cow Mastitis”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 58, no. 1, 2011, pp. 17-20, doi:10.1501/Vetfak_0000002443.
Vancouver Cengiz S, Tekin O, Akan M. Molecular typing of Enterococcus spp. isolated from cow mastitis. Ankara Univ Vet Fak Derg. 2011;58(1):17-20.